MD11G1157400.v1.1

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0007319
Physical Location & Seq
Forward (+)
15187872 .. 15197193
9322 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1157400.v1.1.491

Sequence Viewer

Length: 4326 bp
ATGGCCGAACTGAAGGTCTACCAGAGGAGAGAACCTGATTCCAGAGACAATGCCATGGAAGAAGAAGAGAAAGAGGAGGTAGTTCAGAATCGGAAGGAGGGTTTGTGGGTGCCACAAACCCCTGCAAAGCCTGCTACAGCGAGGACACAAAAATCATATTTCAGAAGACGATTCAAGAGCAATTCGGGACAAAATAATAGCATAGTGATGAGTGATTCCCGAGTGGAATTGGAGCGTTCTGATGGGGTTTCTGTGTTTTCTGCTGCGGCAACTTCTTCAGAGGAAAAGACTTGCCTGATCGACCCAATTGAAGAATGTCCTAGAGAGGAGGAAAAGGTGCAGAATTTGGGTGGAATTGGGAGCACAAATTTGAGTTGTGTTGGGTTGTTCGATTTGAATAAGATTGTGTTGGAATGTGATGAGGGTTGTGTGAGTTTTTCTTATGGGGGTTCCGAGAAAGGTTGTTTAGAGGGATTAGTGGCAGCAGAAGAGGTTGCGGCGAATGATGATGAAATTTTGACAGTTGGGGAGGAGCTGGTGTTGCAGGGGATAATTGTAGAACCTCCAATTTCTCAGTCATCAACAAATGCAACTAAAGGGGCTTTATTTGAAAATATAAACTGTTGTTTCATTCCAGTTGCGCAAACTGGTATCAATGAAGAAAAGCTGCAGCAGGGGTCTGCCATTCCAGATTGTATGTCGCAATGCAAGTCCGGTGATCAACAAAATCAAGAAAGCGTTAAGCAGGAGGATGTCATCCATCGGGCAGAAAACAAGAATCGGACATCCAATGCAGCAAATTTTATGGGAGGCTCCAAATTTCCTAGCACTTCGAGCCCATCACAGAAAAAGAAAAACTTGAAAAGAAGCCCAGATGGTGGTATAGACTTGAATGAAGGGCCACACAAGAAACCGAAGAAGAAAGTATATAGGCCAAAAGTGGTATGTATAGGAAGACCAAGGCCTATAGAGCCTAAGACCCCAAAGAAGGCAACACCAAAGCGTCCTGCTTCAAAACCAAAGACACCAAAGCCTACAGCTAAAATGCATGTTTCTTCCGAGCAAAATATGTCAGCCGAGAAGTCTTCAAGCAGCCCCAAACACTCGGTCCCCATAGAAGTCACCTTGGATAAAGTTGCATTTTCTGTATCAGACAGAGCAACTCTAGATGGTGACCTGAAATCTAAATCAAACACGGAGAATGTCATCATTCTGGCGAATCCTCCGCAAAAAGTTAGAGCTAGTCATGGAACACTTATGAATCTCAGTTGTAAAAAAAATTTAGGACTGAACATTCCAGCTACATGCAGGAAACAAAGGTTGGCGAGGAGGAGGCGGGAAGAAGATAGATGGTCGGCCATAGCTAGATATTTTCTTAAGAGTGAAAGAAACCTTCACAAAGGGCTTGGTGACAAAAATCAGGGACGGGTTTCATTCCAAAAAACAGAAACCACAGATCTAATTGGTGCACTGCAGCCACTAATTACAACAAGGAAGAAAAGATCAAAGGGACATACTCGGGGACGAAATGGAGGGTTGGTGACAGAGAAATGGACTAACATGGGACATTCCCTTAATTTAGAGCTTCACAATGGTGAATGTGAAGGACATCCATCTAAGCATGAGGGCCCTTGCTCCCCCAACATAAGAGATTCACAGGAAGATAATATCGGTCCGTTTGTACAGAAATGTCCAGATATCAGCCATGGAGTTAACCATGACAAAGATGCACATGTTCCCAGCAATGACCATGGCATTCTAGTTCCATACAAGGAGAAGCACAGTAAAAGGCGTCCAGAGCTTTCTCCCACAGACCTTGAACGAGTTAAGTCATGGAAACTTCAATTACAAGCGAAGGTTAATGAGGGTCAAGAGAAAGAAAGTGAAGAGGAATGGTGGACGAATGAAAGAGAAGTCTTACATGGACGGATTAATCTACTTATATCCCGGATGAACACAATCCAAGGAGATAGGAAATTTTCACCATGGAAAGGTTCTGTGGTGGACTCAATAGTTGGAGTTTTCCTAACTCAAAATGTGTCGGATCATCTTTCAAGCAACGCCTTCATGTCACTTGCTGCCAGATTCCCTTGTCAGTCCCATTCCAACGAAACAGATTGTGAATACATAAACATGGTTGGCACCCAAGAATCAGTTGGAAGTAATATTCTTGCTATCATGCCAAAATCTGAGGAGGAGTTACACACTGGTGAAGTGGCACTCTCAATGGACTATAGACACAAAGAGTCAAGCTCTGACAAACCCAATGACCTTGAAGGGGCGTCAACGCCTCCTTGTGATTTCCACAACCCTCCTGAGGTTTCCCAAATTGGTTTCAGTGAAAAGGTAAATCATCTCAGAGTCTTGGAAGATAATGTTGTGTCAGATAACTGTGGAGTTCAGGTAATCTCCGAAAAAGTTGAGTTTCATACATCTGTAGAAATGTCTAGTCTGTCACCACCGTGTTCCCAAACAAAAGATGCAATTGTAGAACCAAACGATGAGAGTCATCTTTGTTCTCAGAATATGCTGGTGGATAAGCCTGAGAGCGCAGTTGATACTATAATCCAAAATCTTGTGGAACCACCTAATGAGGCCAACAACTTCAACCAGGTAAATCAGAAAGGACAGAAACCGGATTTAAATGAGCAACAATCAGGCATGAAATTTGATAAGACGATGAAAGCTCCTCGAAGAAGAAAAAACGAGAAGGCTATAGTGAAGACTAAACTTAGTTGGGGGTGGTGTAAAAGTATATTTTCAACCAGTAGAGAAAGAAATAGGAATCACATGGATTCAGTAGACTGGGAGGCAGTTAGAATTGCAGAAGTTGGTCAGATTGCTGCGGCTATTAAAGTGCGTGGCCAACACAATATGATTGCAGGACGAGTCAAGAAATTTCTTAACCAAGTACATGAGGATCATAAACAAATAGACCTCGAGTGGTTAAGAAATGCCCCGCCTAAGTTAGTAACGAAGTACCTAAGAGAAATTGACGGCATTGGATTGAAAAGCGTGGAGTGTGTGAGACTTTTGGCACTCCAACATGTAGCTTTCCCGGTGGATGTAAATGTTGGCCGGATAGCAATTCGTCTAGGGTGGGCTCCCCTTGAACCATTGCCTGAACAAGTTCAACTACATGTATTAAAACAGATGCCACTGTTGGATACCATTCAGAAGTATCTTTGGCCACGGCTAAAAACCTTGGATCCAAAAACATTGTACGAACTACATTACCAAATGATAACCTTTGGAAAGGTCTTTTGTACAAAGAAAAAGCCAAATTGCAATTCTTGTCCAATGAAGGGAGAGTGCAGACATTACGCTAGTGCATTTGCAAGCGCCAGGCTTGCCTTACCAGGGCCAGAAAAGAAGCCAGGTTCAACAAAGAACGGTAGTTCTACAGATCCCTTGATGATGGCTCTTCAGGCACTCAATCCACCAACTGTCTCCTCTCTTGAGGTCAGCCTTGACTCAAAATACCAAGGCAAAAGTTGTGAACCTATCATTGAGGAACCATCATCACCGCAGCCTGAATATACAGAAGCATCATTAAGAGACATTGAAGATCTTTTTCTTGATGACCCTAATGACATGCCAACAATTAAAATGAGAGATGAAAGATTTTCAACAACATTAGAGCCATACACGGGTGTATTTCAAGAAAACGATATGGCAACAGGATTAGTTCCTTCCCGATATGCAAATTTTCGAGCTCCCAAAGCGAAGGATGCTTTCCGCTTATGCACAAAGCACCTAGTCTATGTTCTTCCAGATTATCATCCTCTATTGCAGACTCTAGAAAACAGAGATCCCGATGATCCAAGCCAATACCTTCTTGCTATATGGCCATCAGATGAAAAACGATGCGATCCTCGGGAGTCCAGAGAATTGAGCAATATTGTGGAGCCATGTTTTTCACTTAATAATCATTCACAAGCAGCTTCACAAATGGTTCGTGGAACACTTTTGATACCGTGTCGAACTGCAACAAGGGGGAGCTTTCCACTCAACGGAACATACTTTCAAGTGAATGAGATATTTGCTGACCATGAAACCAGTATACGCCCGATTGAAGTTCCTAGATCAAGCTTATGGAGTCTAGCTAGGTTAACTGTATACTGTGGGACCTCAACATCTTCAATCCTTAAAGGGTTACCATTGAACGAAATCAAGGACTGCTTTGTGAAAGGTTTTATCTGTGTCCGAGCCTTTAACCGGAAAACACGCACTCCTGAAGAACTTTCAGAGAGATTCCACGTTTCAACAACTAAGAAGGGAGGCAAGGGAGGCAAGGGAGGCAAGAAGAACAAAAAAAAAGGATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1442

Amino Acids

161.07

Weight (kDa)

8.51

Isoelectric Point (pI)

50.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1321 - 1421 1.7e-47 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 642
AccB1I GGYRCC 2 cut(s) 109, 2141
AccB7I CCANNNNNTGG 1 cut(s) 878
AccI GTMKAC 4 cut(s) 18, 2802, 4063, 4119
AciI CCGC 8 cut(s) 266, 497, 1226, 1336, 2846, 2960, 3527, 3739
AclWI GGATC 8 cut(s) 2052, 2928, 3203, 3216, 3401, 3806, 3815, 3866
AcoI YGGCCR 6 cut(s) 3, 1356, 2863, 3076, 3188, 3848
AcuI CTGAAG 4 cut(s) 32, 261, 3410, 4257
AcyI GRCGYC 2 cut(s) 1792, 2282
AfaI GTAC 5 cut(s) 1683, 2913, 2981, 3224, 3268
AflII CTTAAG 1 cut(s) 1376
AflIII ACRYGT 3 cut(s) 1732, 3046, 3139
AjnI CCWGG 4 cut(s) 2610, 3344, 3358, 3376
AjuI GAANNNNNNNTTGG 4 cut(s) 1304, 1336, 3274, 3306
AleI CACNNNNGTG 3 cut(s) 1593, 2208, 2461
Alw21I GWGCWC 3 cut(s) 365, 1471, 3718
Alw26I GTCTC 4 cut(s) 39, 3022, 3454, 3552
Alw44I GTGCAC 1 cut(s) 1467
AlwI GGATC 8 cut(s) 2052, 2928, 3203, 3216, 3401, 3806, 3815, 3866
Ama87I CYCGRG 4 cut(s) 219, 1518, 2939, 3876
ApaI GGGCCC 1 cut(s) 1631
ApaLI GTGCAC 1 cut(s) 1467
ArsI GACNNNNNNTTYG 3 cut(s) 32, 1092, 1124
AseI ATTAAT 1 cut(s) 1932
Asp700I GAANNNNTTC 1 cut(s) 3129
AspLEI GCGC 3 cut(s) 643, 2552, 3344
AspS9I GGNCC 7 cut(s) 899, 1108, 1627, 1628, 1673, 3362, 4128
AsuC2I CCSGG 2 cut(s) 1948, 3059
AvaI CYCGRG 4 cut(s) 219, 1518, 2939, 3876
AvaII GGWCC 3 cut(s) 1108, 1673, 4128
AxyI CCTNAGG 1 cut(s) 2316
BaeGI GKGCMC 2 cut(s) 1471, 1631
BalI TGGCCA 3 cut(s) 2865, 3190, 3850
BamHI GGATCC 1 cut(s) 3208
BanI GGYRCC 2 cut(s) 109, 2141
BanII GRGCYC 4 cut(s) 839, 1631, 3106, 3718
BbsI GAAGAC 4 cut(s) 172, 961, 1077, 2729
Bbv12I GWGCWC 3 cut(s) 365, 1471, 3718
BceAI ACGGC 2 cut(s) 3013, 3209
BciT130I CCWGG 4 cut(s) 2612, 3346, 3360, 3378
BciVI GTATCC 1 cut(s) 3160
BclI TGATCA 1 cut(s) 718
BcnI CCSGG 2 cut(s) 1948, 3059
BcoDI GTCTC 4 cut(s) 39, 3022, 3454, 3552
BfmI CTRYAG 9 cut(s) 135, 668, 966, 1035, 1472, 2233, 2436, 2715, 3402
BfoI RGCGCY 1 cut(s) 3345
BfrI CTTAAG 1 cut(s) 1376
BfuI GTATCC 1 cut(s) 3160
BglII AGATCT 2 cut(s) 1456, 3568
Bme1390I CCNGG 6 cut(s) 1948, 2612, 3059, 3346, 3360, 3378
Bme18I GGWCC 3 cut(s) 1108, 1673, 4128
BmeT110I CYCGRG 4 cut(s) 219, 1518, 2939, 3876
BmgT120I GGNCC 7 cut(s) 899, 1108, 1627, 1628, 1673, 3362, 4128
BmrFI CCNGG 6 cut(s) 1948, 2612, 3059, 3346, 3360, 3378
BmrI ACTGGG 1 cut(s) 2815
BmsI GCATC 6 cut(s) 1717, 2470, 3144, 3557, 3721, 3857
BmuI ACTGGG 1 cut(s) 2815
BpiI GAAGAC 4 cut(s) 172, 961, 1077, 2729
BplI GAGNNNNNCTC 2 cut(s) 2237, 2269
BpuEI CTTGAG 1 cut(s) 3479
BpuMI CCSGG 2 cut(s) 1948, 3059
BsaBI GATNNNNATC 1 cut(s) 3780
BsaHI GRCGYC 2 cut(s) 1792, 2282
BsaWI WCCGGW 3 cut(s) 713, 2635, 4218
Bse1I ACTGG 6 cut(s) 635, 652, 2212, 2766, 2810, 4059
Bse21I CCTNAGG 1 cut(s) 2316
Bse3DI GCAATG 3 cut(s) 710, 1750, 3116
Bse8I GATNNNNATC 1 cut(s) 3780
BseBI CCWGG 4 cut(s) 2612, 3346, 3360, 3378
BseGI GGATG 8 cut(s) 756, 757, 785, 1609, 1956, 3070, 3736, 3781
BseJI GATNNNNATC 1 cut(s) 3780
BseMI GCAATG 3 cut(s) 710, 1750, 3116
BseMII CTCAG 7 cut(s) 587, 1279, 2181, 2307, 2371, 2534, 2535
BseNI ACTGG 6 cut(s) 635, 652, 2212, 2766, 2810, 4059
BseSI GKGCMC 2 cut(s) 1471, 1631
BseYI CCCAGC 1 cut(s) 1739
BsgI GTGCAG 2 cut(s) 359, 3334
BshNI GGYRCC 2 cut(s) 109, 2141
BsiHKAI GWGCWC 3 cut(s) 365, 1471, 3718
BsiHKCI CYCGRG 4 cut(s) 219, 1518, 2939, 3876
BsiSI CCGG 6 cut(s) 714, 1948, 2636, 3059, 3079, 4219
BslFI GGGAC 8 cut(s) 201, 1094, 1437, 1524, 1536, 1578, 2083, 4141
BsmAI GTCTC 4 cut(s) 39, 3022, 3454, 3552
BsmFI GGGAC 8 cut(s) 201, 1094, 1437, 1524, 1536, 1578, 2083, 4141
BsmI GAATGC 1 cut(s) 1755
BsoBI CYCGRG 4 cut(s) 219, 1518, 2939, 3876
Bsp120I GGGCCC 1 cut(s) 1627
Bsp1286I GDGCHC 6 cut(s) 365, 839, 1471, 1631, 3106, 3718
Bsp1407I TGTACA 2 cut(s) 1681, 3266
Bsp19I CCATGG 4 cut(s) 54, 1705, 1750, 1985
BspACI CCGC 8 cut(s) 266, 497, 1226, 1336, 2846, 2960, 3527, 3739
BspCNI CTCAG 7 cut(s) 586, 1278, 2182, 2308, 2370, 2533, 2536
BspMAI CTGCAG 2 cut(s) 672, 1476
BspPI GGATC 8 cut(s) 2052, 2928, 3203, 3216, 3401, 3806, 3815, 3866
BspQI GCTCTTC 1 cut(s) 3429
BspT107I GGYRCC 2 cut(s) 109, 2141
BspTI CTTAAG 1 cut(s) 1376
BsrDI GCAATG 3 cut(s) 710, 1750, 3116
BsrGI TGTACA 2 cut(s) 1681, 3266
BsrI ACTGG 6 cut(s) 635, 652, 2212, 2766, 2810, 4059
BssNAI GTATAC 2 cut(s) 4064, 4120
BssNI GRCGYC 2 cut(s) 1792, 2282
BssT1I CCWWGG 9 cut(s) 54, 959, 1125, 1705, 1750, 1963, 1985, 3204, 3484
Bst1107I GTATAC 2 cut(s) 4064, 4120
Bst2UI CCWGG 4 cut(s) 2612, 3346, 3360, 3378
Bst6I CTCTTC 4 cut(s) 60, 483, 1881, 3429
BstACI GRCGYC 2 cut(s) 1792, 2282
BstAFI CTTAAG 1 cut(s) 1376
BstAPI GCANNNNNTGC 1 cut(s) 131
BstAUI TGTACA 2 cut(s) 1681, 3266
BstC8I GCNNGC 3 cut(s) 132, 3340, 3351
BstDSI CCRYGG 5 cut(s) 54, 1705, 1750, 1985, 3191
BstEII GGTNACC 2 cut(s) 1172, 4155
BstF5I GGATG 8 cut(s) 756, 757, 785, 1609, 1956, 3070, 3736, 3781
BstH2I RGCGCY 1 cut(s) 3345
BstHHI GCGC 3 cut(s) 643, 2552, 3344
BstMAI GTCTC 4 cut(s) 39, 3022, 3454, 3552
BstNI CCWGG 4 cut(s) 2612, 3346, 3360, 3378
BstNSI RCATGY 6 cut(s) 1052, 1308, 1736, 3050, 3143, 3598
BstPI GGTNACC 2 cut(s) 1172, 4155
BstSCI CCNGG 6 cut(s) 1946, 2610, 3057, 3344, 3358, 3376
BstSFI CTRYAG 9 cut(s) 135, 668, 966, 1035, 1472, 2233, 2436, 2715, 3402
BstSLI GKGCMC 2 cut(s) 1471, 1631
BstV2I GAAGAC 4 cut(s) 172, 961, 1077, 2729
BstX2I RGATCY 5 cut(s) 1456, 3208, 3406, 3568, 3811
BstYI RGATCY 5 cut(s) 1456, 3208, 3406, 3568, 3811
BstZ17I GTATAC 2 cut(s) 4064, 4120
Bsu36I CCTNAGG 1 cut(s) 2316
BsuI GTATCC 1 cut(s) 3160
BtgI CCRYGG 5 cut(s) 54, 1705, 1750, 1985, 3191
BtsCI GGATG 8 cut(s) 756, 757, 785, 1609, 1956, 3070, 3736, 3781
BtsI GCAGTG 1 cut(s) 1469
BtsIMutI CAGTG 4 cut(s) 1469, 2205, 2344, 3158
Cac8I GCNNGC 3 cut(s) 132, 3340, 3351
CfoI GCGC 3 cut(s) 643, 2552, 3344
Cfr13I GGNCC 7 cut(s) 899, 1108, 1627, 1628, 1673, 3362, 4128
CpoI CGGWCCG 1 cut(s) 1673
CseI GACGC 3 cut(s) 992, 1781, 2271
CsiI ACCWGGT 1 cut(s) 2610
Csp6I GTAC 5 cut(s) 1682, 2912, 2980, 3223, 3267
CspCI CAANNNNNGTGG 2 cut(s) 1798, 1833
CspI CGGWCCG 1 cut(s) 1673
CviQI GTAC 5 cut(s) 1682, 2912, 2980, 3223, 3267
DraI TTTAAA 1 cut(s) 2643
EaeI YGGCCR 6 cut(s) 3, 1356, 2863, 3076, 3188, 3848
Eam1104I CTCTTC 4 cut(s) 60, 483, 1881, 3429
EarI CTCTTC 4 cut(s) 60, 483, 1881, 3429
Ecl136II GAGCTC 1 cut(s) 3716
Eco130I CCWWGG 9 cut(s) 54, 959, 1125, 1705, 1750, 1963, 1985, 3204, 3484
Eco147I AGGCCT 1 cut(s) 964
Eco24I GRGCYC 4 cut(s) 839, 1631, 3106, 3718
Eco32I GATATC 1 cut(s) 1699
Eco47I GGWCC 3 cut(s) 1108, 1673, 4128
Eco53kI GAGCTC 1 cut(s) 3716
Eco57I CTGAAG 4 cut(s) 32, 261, 3410, 4257
Eco81I CCTNAGG 1 cut(s) 2316
Eco88I CYCGRG 4 cut(s) 219, 1518, 2939, 3876
Eco91I GGTNACC 2 cut(s) 1172, 4155
EcoICRI GAGCTC 1 cut(s) 3716
EcoO109I RGGNCCY 3 cut(s) 1627, 1628, 4128
EcoO65I GGTNACC 2 cut(s) 1172, 4155
EcoRII CCWGG 4 cut(s) 2610, 3344, 3358, 3376
EcoRV GATATC 1 cut(s) 1699
EcoT14I CCWWGG 9 cut(s) 54, 959, 1125, 1705, 1750, 1963, 1985, 3204, 3484
EcoT22I ATGCAT 1 cut(s) 1050
EcoT38I GRGCYC 4 cut(s) 839, 1631, 3106, 3718
ErhI CCWWGG 9 cut(s) 54, 959, 1125, 1705, 1750, 1963, 1985, 3204, 3484
FalI AAGNNNNNCTT 4 cut(s) 842, 874, 4166, 4198
FaqI GGGAC 8 cut(s) 201, 1094, 1437, 1524, 1536, 1578, 2083, 4141
FauI CCCGC 2 cut(s) 1329, 2967
FbaI TGATCA 1 cut(s) 718
FblI GTMKAC 4 cut(s) 18, 2802, 4063, 4119
FokI GGATG 8 cut(s) 743, 764, 772, 1596, 1963, 3077, 3743, 3768
FriOI GRGCYC 4 cut(s) 839, 1631, 3106, 3718
FspI TGCGCA 1 cut(s) 642
GlaI GCGC 3 cut(s) 642, 2551, 3343
GsaI CCCAGC 1 cut(s) 1743
HaeII RGCGCY 1 cut(s) 3345
HapII CCGG 6 cut(s) 714, 1948, 2636, 3059, 3079, 4219
HgaI GACGC 3 cut(s) 992, 1781, 2271
HhaI GCGC 3 cut(s) 643, 2552, 3344
Hin1I GRCGYC 2 cut(s) 1792, 2282
Hin6I GCGC 3 cut(s) 641, 2550, 3342
HinP1I GCGC 3 cut(s) 641, 2550, 3342
HincII GTYRAC 3 cut(s) 1714, 2286, 4113
HindII GTYRAC 3 cut(s) 1714, 2286, 4113
HindIII AAGCTT 1 cut(s) 4090
HpaI GTTAAC 2 cut(s) 1714, 4113
HpaII CCGG 6 cut(s) 714, 1948, 2636, 3059, 3079, 4219
HpyCH4IV ACGT 1 cut(s) 4260
HpySE526I ACGT 1 cut(s) 4260
Hsp92I GRCGYC 2 cut(s) 1792, 2282
HspAI GCGC 3 cut(s) 641, 2550, 3342
Ksp22I TGATCA 1 cut(s) 718
KspAI GTTAAC 2 cut(s) 1714, 4113
LguI GCTCTTC 1 cut(s) 3429
LweI GCATC 6 cut(s) 1717, 2470, 3144, 3557, 3721, 3857
MabI ACCWGGT 1 cut(s) 2610
MaeII ACGT 1 cut(s) 4260
MaeIII GTNAC 9 cut(s) 1120, 1172, 1409, 1540, 2070, 2199, 2454, 2971, 4155
MfeI CAATTG 2 cut(s) 306, 2484
MflI RGATCY 5 cut(s) 1456, 3208, 3406, 3568, 3811
MhlI GDGCHC 6 cut(s) 365, 839, 1471, 1631, 3106, 3718
MlsI TGGCCA 3 cut(s) 2865, 3190, 3850
MluNI TGGCCA 3 cut(s) 2865, 3190, 3850
MlyI GAGTC 9 cut(s) 2000, 2255, 2370, 2515, 2898, 3467, 3790, 3890, 4108
MmeI TCCRAC 7 cut(s) 390, 1996, 2022, 2130, 2137, 3067, 3144
Mox20I TGGCCA 3 cut(s) 2865, 3190, 3850
Mph1103I ATGCAT 1 cut(s) 1050
MroXI GAANNNNTTC 1 cut(s) 3129
MscI TGGCCA 3 cut(s) 2865, 3190, 3850
MslI CAYNNNNRTG 5 cut(s) 206, 1593, 2132, 2208, 2461
Msp20I TGGCCA 3 cut(s) 2865, 3190, 3850
MspCI CTTAAG 1 cut(s) 1376
MspI CCGG 6 cut(s) 714, 1948, 2636, 3059, 3079, 4219
MspR9I CCNGG 6 cut(s) 1948, 2612, 3059, 3346, 3360, 3378
MunI CAATTG 2 cut(s) 306, 2484
Mva1269I GAATGC 1 cut(s) 1755
MvaI CCWGG 4 cut(s) 2612, 3346, 3360, 3378
NciI CCSGG 2 cut(s) 1948, 3059
NcoI CCATGG 4 cut(s) 54, 1705, 1750, 1985
NmeAIII GCCGAG 1 cut(s) 1102
NmuCI GTSAC 6 cut(s) 1120, 1172, 1409, 1540, 2070, 2454
NsbI TGCGCA 1 cut(s) 642
NsiI ATGCAT 1 cut(s) 1050
NspI RCATGY 6 cut(s) 1052, 1308, 1736, 3050, 3143, 3598
OliI CACNNNNGTG 3 cut(s) 1593, 2208, 2461
PaeR7I CTCGAG 1 cut(s) 2939
PceI AGGCCT 1 cut(s) 964
PciI ACATGT 3 cut(s) 1732, 3046, 3139
PciSI GCTCTTC 1 cut(s) 3429
PctI GAATGC 1 cut(s) 1755
PdmI GAANNNNTTC 1 cut(s) 3129
PflMI CCANNNNNTGG 1 cut(s) 878
PfoI TCCNGGA 1 cut(s) 1946
PleI GAGTC 9 cut(s) 2000, 2254, 2369, 2514, 2897, 3467, 3790, 3889, 4107
PpsI GAGTC 9 cut(s) 2000, 2254, 2369, 2514, 2897, 3467, 3790, 3889, 4107
PpuMI RGGWCCY 1 cut(s) 4128
PscI ACATGT 3 cut(s) 1732, 3046, 3139
PshBI ATTAAT 1 cut(s) 1932
Psp124BI GAGCTC 1 cut(s) 3718
Psp5II RGGWCCY 1 cut(s) 4128
Psp6I CCWGG 4 cut(s) 2610, 3344, 3358, 3376
PspEI GGTNACC 2 cut(s) 1172, 4155
PspFI CCCAGC 1 cut(s) 1739
PspGI CCWGG 4 cut(s) 2610, 3344, 3358, 3376
PspOMI GGGCCC 1 cut(s) 1627
PspPI GGNCC 7 cut(s) 899, 1108, 1627, 1628, 1673, 3362, 4128
PspPPI RGGWCCY 1 cut(s) 4128
PspXI VCTCGAGB 1 cut(s) 2939
PstI CTGCAG 2 cut(s) 672, 1476
PsuI RGATCY 5 cut(s) 1456, 3208, 3406, 3568, 3811
RsaI GTAC 5 cut(s) 1683, 2913, 2981, 3224, 3268
RsaNI GTAC 5 cut(s) 1682, 2912, 2980, 3223, 3267
RseI CAYNNNNRTG 5 cut(s) 206, 1593, 2132, 2208, 2461
Rsr2I CGGWCCG 1 cut(s) 1673
RsrII CGGWCCG 1 cut(s) 1673
SacI GAGCTC 1 cut(s) 3718
SapI GCTCTTC 1 cut(s) 3429
Sau96I GGNCC 7 cut(s) 899, 1108, 1627, 1628, 1673, 3362, 4128
SchI GAGTC 9 cut(s) 2000, 2255, 2370, 2515, 2898, 3467, 3790, 3890, 4108
ScrFI CCNGG 6 cut(s) 1948, 2612, 3059, 3346, 3360, 3378
SduI GDGCHC 6 cut(s) 365, 839, 1471, 1631, 3106, 3718
SexAI ACCWGGT 1 cut(s) 2610
SfaNI GCATC 6 cut(s) 1717, 2470, 3144, 3557, 3721, 3857
SfcI CTRYAG 9 cut(s) 135, 668, 966, 1035, 1472, 2233, 2436, 2715, 3402
Sfr274I CTCGAG 1 cut(s) 2939
SinI GGWCC 3 cut(s) 1108, 1673, 4128
SlaI CTCGAG 1 cut(s) 2939
SmiI ATTTAAAT 1 cut(s) 2643
SmiMI CAYNNNNRTG 5 cut(s) 206, 1593, 2132, 2208, 2461
SmlI CTYRAG 3 cut(s) 1376, 2939, 3458
SmoI CTYRAG 3 cut(s) 1376, 2939, 3458
SseBI AGGCCT 1 cut(s) 964
SsiI CCGC 8 cut(s) 266, 497, 1226, 1336, 2846, 2960, 3527, 3739
SspI AATATT 2 cut(s) 2167, 3901
SstI GAGCTC 1 cut(s) 3718
StuI AGGCCT 1 cut(s) 964
StyD4I CCNGG 6 cut(s) 1946, 2610, 3057, 3344, 3358, 3376
StyI CCWWGG 9 cut(s) 54, 959, 1125, 1705, 1750, 1963, 1985, 3204, 3484
SwaI ATTTAAAT 1 cut(s) 2643
TaiI ACGT 1 cut(s) 4263
TaqI TCGA 7 cut(s) 300, 390, 833, 2692, 2940, 3712, 3982
TaqII GACCGA 2 cut(s) 1096, 1661
TatI WGTACW 3 cut(s) 1681, 2911, 3266
TauI GCSGC 3 cut(s) 269, 500, 2849
TscAI CASTG 4 cut(s) 1476, 2212, 2344, 3165
TseFI GTSAC 6 cut(s) 1120, 1172, 1409, 1540, 2070, 2454
Tsp45I GTSAC 6 cut(s) 1120, 1172, 1409, 1540, 2070, 2454
TspGWI ACGGA 4 cut(s) 1211, 1665, 1942, 4029
TspRI CASTG 4 cut(s) 1476, 2212, 2344, 3165
Van91I CCANNNNNTGG 1 cut(s) 878
Vha464I CTTAAG 1 cut(s) 1376
VneI GTGCAC 1 cut(s) 1467
VpaK11BI GGWCC 3 cut(s) 1108, 1673, 4128
VspI ATTAAT 1 cut(s) 1932
XbaI TCTAGA 2 cut(s) 1165, 3799
XceI RCATGY 6 cut(s) 1052, 1308, 1736, 3050, 3143, 3598
XcmI CCANNNNNNNNNTGG 1 cut(s) 2153
XhoI CTCGAG 1 cut(s) 2939
XmiI GTMKAC 4 cut(s) 18, 2802, 4063, 4119
XmnI GAANNNNTTC 1 cut(s) 3129
Zsp2I ATGCAT 1 cut(s) 1050
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.