RLG00000025288

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0007319
Physical Location & Seq
Reverse (-)
43620185 .. 43630432
10248 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000025288

Sequence Viewer

Length: 3723 bp
ATGGAACGGGTTCAGGTTCAGATCCCAGATACTCCTGCAAAGAAAACTCAGAGACAACGTTCTAGCGGGATTTCTATGCTTACTGCCCCTACAAATTCTTTAGAGGAAAACAAAGAGAACCCGATTGCAGAGGACTTGTCTGCAATGGACGACGAGGTAGTCGATTACAGTGGACTTATGGACACGATTTTGGATTCCATTGGTTCCTTGGATTTGAACAAGCCTGTGAGGGAGGAGTGGGAGACTGAGGAATTTGCTTATGGTTATAGGGCCGCATTATTGCAAACAGAAGATCTTGCAAGGGAGGATGAGGAAGTGTTGACATATAATCTTCCAAGTTCTCAGTCATCTTCCAATACCGAAAATCCTGAAAATCACAGTTGCGGGTTTGGCAGCATAGAGGGTTCCTTCATGCCAGTTGTGCAACCTGGTTATGCTGCTGAATCCATATCACAATATAATTTGAAGAATCAGGAATTAGTTGATGGCAAGTACAATATTGCTGATACAAATTATATTGGTAGCTTCAAAAGAAAACTACATGGTGCTACACTTCAAATGCCACGGAAGAGACTCAAGAGGCCGAAGAAAAAAGTTTACCGGCCAAAAGTGGTACGTAATTTAGGAAGGCCTATACCTAGAATGGAACCCAAGACTCCCACCAAAAAGAATGAAAGGATGAAGCTCAAAACACCAAAAATGAAGAAAAATGTGTCATTGAAGAAGTCTTCGAGCACAATCAACCTCAACCATGTCCGAGTTCCTTTAGATTTGGAGCCTAGTGTATCAGAGGGAGAATTTTCAAGCATTGACATAGAAAAGCACTCTAACGATGGAACCCGCATCATATCAGAGAAGACAGTGGAGCATTGCATCACAAAATGCATTGATTTTGATATAGGATTAAATTTTTTGACGAAGAAAAAGAGGACGACGATGGGAAAGAAATGGGAAGTGAAAGACTATAGTAGAATAATGGAACCGAATCTTCTGAAAATAAAAAAGAAAAAAAGGATGGGCAGGAGGAGATGGGAAGTAGACGTGTGGAAAAAAATAGCTAGCAAGTTTCAGACAAACTATCTTAAACGGAGACGCAATAAAAAGAGAAGAGCTGCCCCCGAAATCAGTCAACAGGAAGTGCGCAACATCTTTAACACCGAAAAGCAGTTGTCTCCATGCTTCATCAGCGAATCAGGAGATATACCTGAAGATAATATCAATGTTGTTGTGCAAAAACATCAACTTAACCATGGAATACTTGTTTGTTACCAAGAAAATGAGAAACATGGTCCTCATGTTGAGCTGGGTATCGTAGCCAGTGCAAATGGTGAGCTTGGTATGGTAGCCACGACAAAGGGAAAGGGAAGGAGGCAAGAAAAGCTTGAGAAATTAGAACATGGTGAACTAGCTCACCCATGGAAGCTTCAATTGCAAAATGAAGTTAAGGAGGGGGACAAACATGACCCGAATTCTGAATTTTGGGATAAGGAGAGACAATTCTTTCAGGGTCGGATTAACTTATTTTTAACTCGTATGCGCATAGTTCAAGGAAATAGAAAATTTTCAGAATGGGATGGATCAGTTATGGATTCGATAGTCGGAGTTTTTTTAACACAGAACGTATCAGATCATCTATCCAGTTCTGCCTTTATATCACTCGCTGCAGCTTTCCCATATCAGCCCAGTTGTAACCAAGAATTAACTTATGCAAACTCAGAGCGAAGTTGTGACCAATCAAGAATTGTTAAAATTGAGGAATTAGTAGAGGGTGGAATCTCAAAGTCCAGCTGTGAACAATCAAATGACCAAACATATTGTGAAGATATAAATAGTGGCAGCCATAAATTGGTTGGAAGTAACATGGTATTTGATAAAGAGTCGTTCACACAAGAAGCCACAACTACAATGAAGTACGAAAATCAGAAGTCAAGTGACTGCGAAATGGTATTAGCCAAACAAGTTGAGTTGCAGCAAAATTTTGAGGGTCAAGATTATTACGTGGACATGCCATCTGATAAGCCTGACTGCTCGATTAATATTATAACTGAAAATTCGCTTTCTTACTTCAATGAGGAAAAGCAAAGAGGAAAGAAGTTGAAGGTGGATGCTCGAGAAATAAATAATGAGGGTGCTATGCTGAATGGAAAACCTGATTGGGATTATTATAGGAGAGACATTGGAAAGGAAAGAAAGATCAGGCACATGGCGGATTCGGTAGACTGGCAGGCCGTTAGACAAGCAGATGTTGGTGATGTTGCTGAAGCCATCAAAGGACGTGGACAACATCGTGTTATCGCAGATCGAATAAAGAAATTTCTTAATAACGTGAATCAAGACCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCGGACAAACTAGTAAGGAAATATTTGACTAATATCGAGGGAATTGGCCTGAAAAGTGTAGAGTGCGTGCGCCTTTTAGCACTCAAGCAGATAGCCTTCCCGGTGGACGTTAATGTTGGCAGGATAGCCATTCGTTTAGGGTGGGTGCCTATGCAACCATTACCTGTAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAAAAGTATCTTTGGCCTCGGCTAATGAAATTGAAGTTCGAAGAATTGTATGAACTACACTATCAAATGATAACCTTTGGAAAGGTTTATTGTACCAAACGTAAACCTAACTGTCATTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTTAGTGCATATGCAAGTTCAAAATATGCTGCTTTACCGAAGTCAATGAAGAAGCAAGGACAAAAGAAAACTGATGTGGTTATTTGCAATAATCATCTTTCTTCTGGGTATGAGCCAGTAAATGCAGCATCACAATCATATCTTGAGGAAACTACTAAATTAGAATATCAAATAAAAGATTGTGAACCGATAATTGAAGAACCAGCATCACCTCGACCGTCATTACCGGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGATGATCCTTTTAAAGATTTCGTAAAAAGTCCATATATGAATACATTTCGAGGAAGTGATATCTCAACACTGGTTCCTTTTATGACCCCAGAAGAAGCTGCTTTCATCCCCGCTCGTAGACTAAAAGAAGTGGACCGTTTGAGGACAAAACACTCAGTCTACATGATTAAAGATCAAGGCCCTTTATTTAAAGAGCTACAACTAGAGAGACGTCACCCTCATGATAACAGTCCTTACCTTTTTGCCTTGTGCACATCAGATCAAATGGCAAAAGTATCTCAATCACCTCAAAACAGATGCAGTTGCAAGGGATCTAATGATTCATCATGTGATGGTGAGAAGACATGTTTTTCTTGTAACAGTCTTCCAACACAAGCTAATACTGTTCAAGGAACAATTTTGATACCTTGTAGAACAGCAATGAGGGGAAGTTTTCCATTAAACGGTACATATTTTCAAATTAATGAGGTTTTTGCGGATCATGAATCCAGTATACGCCCCATCAACGTTCATAGAGATTTGTTATGGGATCAGACGAGAAGAGATGTATACTTTGGAACATCAACAAGTGCAATTCTTAAAGGTTTTATTTGCGTTAGAGCATTTAATCGGGAAACACGCAGGCCAGAGCCTCTATTAGAAAGATTTCACATTTCAACCGTCAAAGCCGCAAAGAAAGGAAAGAAAAAAGCGGAACAGAAAGAAGACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1241

Amino Acids

142.35

Weight (kDa)

8.63

Isoelectric Point (pI)

48.96

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1134 - 1221 6.1e-36 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2042
AasI GACNNNNNNGTC 1 cut(s) 158
AatII GACGTC 1 cut(s) 3223
Acc16I TGCGCA 2 cut(s) 1142, 1538
AccB1I GGYRCC 1 cut(s) 2518
AccB7I CCANNNNNTGG 1 cut(s) 1846
AccBSI CCGCTC 1 cut(s) 3122
AccI GTMKAC 6 cut(s) 1038, 2217, 3127, 3168, 3502, 3558
AccIII TCCGGA 1 cut(s) 2374
AciI CCGC 9 cut(s) 66, 273, 384, 841, 2207, 3120, 3485, 3678, 3701
AclI AACGTT 2 cut(s) 58, 3516
AclWI GGATC 6 cut(s) 16, 1585, 3008, 3328, 3495, 3546
AcoI YGGCCR 1 cut(s) 602
AcuI CTGAAG 2 cut(s) 1227, 2280
AcyI GRCGYC 1 cut(s) 3220
AfaI GTAC 5 cut(s) 494, 615, 1913, 2683, 3457
AfiI CCNNNNNNNGG 2 cut(s) 1846, 3452
AflIII ACRYGT 3 cut(s) 1041, 2554, 3353
AhlI ACTAGT 1 cut(s) 2383
AjiI CACGTC 2 cut(s) 1042, 2276
AjnI CCWGG 1 cut(s) 427
AjuI GAANNNNNNNTTGG 2 cut(s) 2137, 2169
Alw21I GWGCWC 2 cut(s) 737, 3263
Alw26I GTCTC 9 cut(s) 46, 236, 565, 1084, 1176, 1486, 2166, 2982, 3211
Alw44I GTGCAC 1 cut(s) 3259
AlwI GGATC 6 cut(s) 16, 1585, 3008, 3328, 3495, 3546
Ama87I CYCGRG 1 cut(s) 2109
Aor13HI TCCGGA 1 cut(s) 2374
AoxI GGCC 9 cut(s) 270, 581, 602, 629, 2226, 2419, 2603, 3187, 3632
ApaLI GTGCAC 1 cut(s) 3259
AseI ATTAAT 2 cut(s) 2034, 3471
Asp700I GAANNNNTTC 3 cut(s) 9, 1561, 3654
AspLEI GCGC 3 cut(s) 1143, 1539, 2445
AspS9I GGNCC 4 cut(s) 270, 1289, 3142, 3188
AsuC2I CCSGG 1 cut(s) 2474
AsuHPI GGTGA 8 cut(s) 1340, 1403, 1412, 2261, 2940, 3215, 3285, 3356
AsuII TTCGAA 1 cut(s) 2628
AsuNHI GCTAGC 1 cut(s) 1058
AvaI CYCGRG 1 cut(s) 2109
AvaII GGWCC 2 cut(s) 1289, 3142
BaeGI GKGCMC 1 cut(s) 3263
BanI GGYRCC 1 cut(s) 2518
BanII GRGCYC 1 cut(s) 2374
BbsI GAAGAC 4 cut(s) 720, 863, 3356, 3365
Bbv12I GWGCWC 2 cut(s) 737, 3263
BceAI ACGGC 1 cut(s) 2213
BciT130I CCWGG 1 cut(s) 429
BcnI CCSGG 1 cut(s) 2474
BcoDI GTCTC 9 cut(s) 46, 236, 565, 1084, 1176, 1486, 2166, 2982, 3211
BcuI ACTAGT 1 cut(s) 2383
BfaI CTAG 7 cut(s) 63, 639, 780, 1059, 1406, 2384, 3212
BfmI CTRYAG 3 cut(s) 964, 1662, 2538
BglII AGATCT 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 429, 2474
Bme18I GGWCC 2 cut(s) 1289, 3142
BmeT110I CYCGRG 1 cut(s) 2109
BmgBI CACGTC 2 cut(s) 1042, 2276
BmgT120I GGNCC 4 cut(s) 270, 1289, 3142, 3188
BmrFI CCNGG 2 cut(s) 429, 2474
BmrI ACTGGG 1 cut(s) 1677
BmsI GCATC 7 cut(s) 852, 882, 2095, 2559, 2876, 2954, 3296
BmtI GCTAGC 1 cut(s) 1062
BmuI ACTGGG 1 cut(s) 1677
BpiI GAAGAC 4 cut(s) 720, 863, 3356, 3365
Bpu14I TTCGAA 1 cut(s) 2628
BpuEI CTTGAG 5 cut(s) 560, 1403, 2375, 2441, 2903
BpuMI CCSGG 1 cut(s) 2474
BsaAI YACGTR 3 cut(s) 617, 1999, 2557
BsaHI GRCGYC 1 cut(s) 3220
BsaJI CCNNGG 5 cut(s) 207, 563, 1249, 1415, 2606
BsaWI WCCGGW 2 cut(s) 2374, 2965
Bsc4I CCNNNNNNNGG 2 cut(s) 1846, 3452
Bse118I RCCGGY 2 cut(s) 600, 2572
Bse1I ACTGG 8 cut(s) 416, 1317, 1638, 1683, 2225, 2855, 3084, 3498
Bse3DI GCAATG 3 cut(s) 150, 868, 3435
BseAI TCCGGA 1 cut(s) 2374
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 5 cut(s) 207, 563, 1249, 1415, 2606
BseGI GGATG 6 cut(s) 313, 684, 1020, 1579, 2110, 3114
BseLI CCNNNNNNNGG 2 cut(s) 1846, 3452
BseMI GCAATG 3 cut(s) 150, 868, 3435
BseMII CTCAG 5 cut(s) 62, 237, 356, 1728, 3177
BseNI ACTGG 8 cut(s) 416, 1317, 1638, 1683, 2225, 2855, 3084, 3498
BseRI GAGGAG 2 cut(s) 248, 1039
BseSI GKGCMC 1 cut(s) 3263
BseYI CCCAGC 1 cut(s) 1303
Bsh1285I CGRYCG 1 cut(s) 2957
BshFI GGCC 9 cut(s) 272, 583, 604, 631, 2228, 2421, 2605, 3189, 3634
BshNI GGYRCC 1 cut(s) 2518
BsiEI CGRYCG 1 cut(s) 2957
BsiHKAI GWGCWC 2 cut(s) 737, 3263
BsiHKCI CYCGRG 1 cut(s) 2109
BsiSI CCGG 5 cut(s) 601, 2375, 2474, 2573, 2966
BslFI GGGAC 1 cut(s) 1466
BslI CCNNNNNNNGG 2 cut(s) 1846, 3452
BsmAI GTCTC 9 cut(s) 46, 236, 565, 1084, 1176, 1486, 2166, 2982, 3211
BsmBI CGTCTC 2 cut(s) 1084, 3211
BsmFI GGGAC 1 cut(s) 1466
BsnI GGCC 9 cut(s) 272, 583, 604, 631, 2228, 2421, 2605, 3189, 3634
BsoBI CYCGRG 1 cut(s) 2109
Bsp119I TTCGAA 1 cut(s) 2628
Bsp1286I GDGCHC 3 cut(s) 737, 2374, 3263
Bsp13I TCCGGA 1 cut(s) 2374
Bsp19I CCATGG 2 cut(s) 1249, 1415
BspACI CCGC 9 cut(s) 66, 273, 384, 841, 2207, 3120, 3485, 3678, 3701
BspANI GGCC 9 cut(s) 272, 583, 604, 631, 2228, 2421, 2605, 3189, 3634
BspCNI CTCAG 5 cut(s) 61, 238, 355, 1727, 3176
BspEI TCCGGA 1 cut(s) 2374
BspHI TCATGA 2 cut(s) 3229, 3490
BspMAI CTGCAG 1 cut(s) 1666
BspOI GCTAGC 1 cut(s) 1062
BspPI GGATC 6 cut(s) 16, 1585, 3008, 3328, 3495, 3546
BspQI GCTCTTC 1 cut(s) 1102
BspT104I TTCGAA 1 cut(s) 2628
BspT107I GGYRCC 1 cut(s) 2518
BsrBI CCGCTC 1 cut(s) 3122
BsrDI GCAATG 3 cut(s) 150, 868, 3435
BsrFI RCCGGY 2 cut(s) 600, 2572
BsrI ACTGG 8 cut(s) 416, 1317, 1638, 1683, 2225, 2855, 3084, 3498
BssAI RCCGGY 2 cut(s) 600, 2572
BssECI CCNNGG 5 cut(s) 207, 563, 1249, 1415, 2606
BssNAI GTATAC 2 cut(s) 3503, 3559
BssNI GRCGYC 1 cut(s) 3220
BssT1I CCWWGG 3 cut(s) 207, 1249, 1415
Bst1107I GTATAC 2 cut(s) 3503, 3559
Bst2UI CCWGG 1 cut(s) 429
Bst6I CTCTTC 4 cut(s) 563, 1102, 2559, 3544
BstACI GRCGYC 1 cut(s) 3220
BstBAI YACGTR 3 cut(s) 617, 1999, 2557
BstBI TTCGAA 1 cut(s) 2628
BstC8I GCNNGC 4 cut(s) 1060, 2226, 2441, 3632
BstDEI CTNAG 7 cut(s) 48, 246, 342, 1714, 2363, 2741, 3163
BstDSI CCRYGG 3 cut(s) 563, 1249, 1415
BstF5I GGATG 6 cut(s) 313, 684, 1020, 1579, 2110, 3114
BstHHI GCGC 3 cut(s) 1143, 1539, 2445
BstMAI GTCTC 9 cut(s) 46, 236, 565, 1084, 1176, 1486, 2166, 2982, 3211
BstMCI CGRYCG 1 cut(s) 2957
BstMWI GCNNNNNNNGC 5 cut(s) 390, 421, 1185, 1378, 1429
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 2 cut(s) 2008, 3357
BstSCI CCNGG 2 cut(s) 427, 2472
BstSFI CTRYAG 3 cut(s) 964, 1662, 2538
BstSLI GKGCMC 1 cut(s) 3263
BstSNI TACGTA 1 cut(s) 617
BstV2I GAAGAC 4 cut(s) 720, 863, 3356, 3365
BstX2I RGATCY 3 cut(s) 21, 292, 3320
BstYI RGATCY 3 cut(s) 21, 292, 3320
BstZ17I GTATAC 2 cut(s) 3503, 3559
BsuRI GGCC 9 cut(s) 272, 583, 604, 631, 2228, 2421, 2605, 3189, 3634
BtgI CCRYGG 3 cut(s) 563, 1249, 1415
BtrI CACGTC 2 cut(s) 1042, 2276
BtsCI GGATG 6 cut(s) 313, 684, 1020, 1579, 2110, 3114
BtsIMutI CAGTG 4 cut(s) 175, 867, 1324, 3077
Cac8I GCNNGC 4 cut(s) 1060, 2226, 2441, 3632
CciI TCATGA 2 cut(s) 3229, 3490
CfoI GCGC 3 cut(s) 1143, 1539, 2445
Cfr10I RCCGGY 2 cut(s) 600, 2572
Cfr13I GGNCC 4 cut(s) 270, 1289, 3142, 3188
CseI GACGC 1 cut(s) 1101
CsiI ACCWGGT 1 cut(s) 427
Csp6I GTAC 5 cut(s) 493, 614, 1912, 2682, 3456
CspCI CAANNNNNGTGG 2 cut(s) 2257, 2292
CviQI GTAC 5 cut(s) 493, 614, 1912, 2682, 3456
DdeI CTNAG 7 cut(s) 48, 246, 342, 1714, 2363, 2741, 3163
DraI TTTAAA 2 cut(s) 3022, 3199
DrdI GACNNNNNNGTC 1 cut(s) 158
DseDI GACNNNNNNGTC 1 cut(s) 158
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 4 cut(s) 563, 1102, 2559, 3544
EarI CTCTTC 4 cut(s) 563, 1102, 2559, 3544
EciI GGCGGA 1 cut(s) 2222
Eco105I TACGTA 1 cut(s) 617
Eco130I CCWWGG 3 cut(s) 207, 1249, 1415
Eco147I AGGCCT 1 cut(s) 631
Eco24I GRGCYC 1 cut(s) 2374
Eco32I GATATC 1 cut(s) 3070
Eco47I GGWCC 2 cut(s) 1289, 3142
Eco57I CTGAAG 2 cut(s) 1227, 2280
Eco88I CYCGRG 1 cut(s) 2109
EcoO109I RGGNCCY 1 cut(s) 3188
EcoRI GAATTC 1 cut(s) 1468
EcoRII CCWGG 1 cut(s) 427
EcoRV GATATC 1 cut(s) 3070
EcoT14I CCWWGG 3 cut(s) 207, 1249, 1415
EcoT22I ATGCAT 1 cut(s) 887
EcoT38I GRGCYC 1 cut(s) 2374
ErhI CCWWGG 3 cut(s) 207, 1249, 1415
Esp3I CGTCTC 2 cut(s) 1084, 3211
FalI AAGNNNNNCTT 2 cut(s) 1365, 1397
FaqI GGGAC 1 cut(s) 1466
FauI CCCGC 4 cut(s) 59, 377, 848, 3127
FauNDI CATATG 1 cut(s) 2749
FblI GTMKAC 6 cut(s) 1038, 2217, 3127, 3168, 3502, 3558
FokI GGATG 6 cut(s) 320, 691, 1027, 1586, 2117, 3101
FriOI GRGCYC 1 cut(s) 2374
FspAI RTGCGCAY 1 cut(s) 1538
FspBI CTAG 7 cut(s) 63, 639, 780, 1059, 1406, 2384, 3212
FspI TGCGCA 2 cut(s) 1142, 1538
GlaI GCGC 3 cut(s) 1142, 1538, 2444
GsaI CCCAGC 1 cut(s) 1307
HaeIII GGCC 9 cut(s) 272, 583, 604, 631, 2228, 2421, 2605, 3189, 3634
HapII CCGG 5 cut(s) 601, 2375, 2474, 2573, 2966
HgaI GACGC 1 cut(s) 1101
HhaI GCGC 3 cut(s) 1143, 1539, 2445
Hin1I GRCGYC 1 cut(s) 3220
Hin6I GCGC 3 cut(s) 1141, 1537, 2443
HinP1I GCGC 3 cut(s) 1141, 1537, 2443
HincII GTYRAC 2 cut(s) 321, 1130
HindII GTYRAC 2 cut(s) 321, 1130
HindIII AAGCTT 2 cut(s) 1379, 1421
HpaII CCGG 5 cut(s) 601, 2375, 2474, 2573, 2966
HphI GGTGA 8 cut(s) 1340, 1403, 1412, 2261, 2940, 3215, 3285, 3356
Hpy99I CGWCG 2 cut(s) 155, 937
HpyAV CCTTC 5 cut(s) 418, 621, 1359, 2092, 2479
HpyF10VI GCNNNNNNNGC 5 cut(s) 390, 421, 1185, 1378, 1429
HpyF3I CTNAG 7 cut(s) 48, 246, 342, 1714, 2363, 2741, 3163
Hsp92I GRCGYC 1 cut(s) 3220
HspAI GCGC 3 cut(s) 1141, 1537, 2443
Kpn2I TCCGGA 1 cut(s) 2374
LguI GCTCTTC 1 cut(s) 1102
LmnI GCTCC 3 cut(s) 775, 865, 2377
LweI GCATC 7 cut(s) 852, 882, 2095, 2559, 2876, 2954, 3296
MabI ACCWGGT 1 cut(s) 427
MaeI CTAG 7 cut(s) 63, 639, 780, 1059, 1406, 2384, 3212
MaeIII GTNAC 7 cut(s) 1265, 1688, 1727, 1856, 1931, 3221, 3365
MbiI CCGCTC 1 cut(s) 3122
MfeI CAATTG 1 cut(s) 1427
MflI RGATCY 3 cut(s) 21, 292, 3320
MhlI GDGCHC 3 cut(s) 737, 2374, 3263
MlyI GAGTC 3 cut(s) 567, 649, 1886
MmeI TCCRAC 4 cut(s) 1490, 1579, 1831, 3401
Mph1103I ATGCAT 1 cut(s) 887
MroI TCCGGA 1 cut(s) 2374
MroXI GAANNNNTTC 3 cut(s) 9, 1561, 3654
MslI CAYNNNNRTG 1 cut(s) 3228
MspA1I CMGCKG 1 cut(s) 1788
MspI CCGG 5 cut(s) 601, 2375, 2474, 2573, 2966
MspR9I CCNGG 2 cut(s) 429, 2474
MunI CAATTG 1 cut(s) 1427
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 5 cut(s) 390, 421, 1185, 1378, 1429
NciI CCSGG 1 cut(s) 2474
NcoI CCATGG 2 cut(s) 1249, 1415
NdeI CATATG 1 cut(s) 2749
NheI GCTAGC 1 cut(s) 1058
NmeAIII GCCGAG 1 cut(s) 2587
NmuCI GTSAC 3 cut(s) 1727, 1931, 3221
NsbI TGCGCA 2 cut(s) 1142, 1538
NsiI ATGCAT 1 cut(s) 887
NspI RCATGY 2 cut(s) 2008, 3357
NspV TTCGAA 1 cut(s) 2628
PaeR7I CTCGAG 1 cut(s) 2109
PagI TCATGA 2 cut(s) 3229, 3490
PceI AGGCCT 1 cut(s) 631
PciI ACATGT 1 cut(s) 3353
PciSI GCTCTTC 1 cut(s) 1102
PcsI WCGNNNNNNNCGW 1 cut(s) 159
PdmI GAANNNNTTC 3 cut(s) 9, 1561, 3654
PflMI CCANNNNNTGG 1 cut(s) 1846
PleI GAGTC 3 cut(s) 567, 649, 1885
PpsI GAGTC 3 cut(s) 567, 649, 1885
Ppu21I YACGTR 3 cut(s) 617, 1999, 2557
PscI ACATGT 1 cut(s) 3353
PshBI ATTAAT 2 cut(s) 2034, 3471
PsiI TTATAA 1 cut(s) 2042
Psp1406I AACGTT 2 cut(s) 58, 3516
Psp6I CCWGG 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 1303
PspGI CCWGG 1 cut(s) 427
PspPI GGNCC 4 cut(s) 270, 1289, 3142, 3188
PstI CTGCAG 1 cut(s) 1666
PsuI RGATCY 3 cut(s) 21, 292, 3320
PvuII CAGCTG 1 cut(s) 1788
RsaI GTAC 5 cut(s) 494, 615, 1913, 2683, 3457
RsaNI GTAC 5 cut(s) 493, 614, 1912, 2682, 3456
RseI CAYNNNNRTG 1 cut(s) 3228
SapI GCTCTTC 1 cut(s) 1102
Sau96I GGNCC 4 cut(s) 270, 1289, 3142, 3188
SchI GAGTC 3 cut(s) 567, 649, 1886
ScrFI CCNGG 2 cut(s) 429, 2474
SduI GDGCHC 3 cut(s) 737, 2374, 3263
SexAI ACCWGGT 1 cut(s) 427
SfaNI GCATC 7 cut(s) 852, 882, 2095, 2559, 2876, 2954, 3296
SfcI CTRYAG 3 cut(s) 964, 1662, 2538
Sfr274I CTCGAG 1 cut(s) 2109
SfuI TTCGAA 1 cut(s) 2628
SinI GGWCC 2 cut(s) 1289, 3142
SlaI CTCGAG 1 cut(s) 2109
SmiMI CAYNNNNRTG 1 cut(s) 3228
SmlI CTYRAG 6 cut(s) 575, 1382, 2109, 2354, 2456, 2882
SmoI CTYRAG 6 cut(s) 575, 1382, 2109, 2354, 2456, 2882
SnaBI TACGTA 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 2383
SseBI AGGCCT 1 cut(s) 631
SsiI CCGC 9 cut(s) 66, 273, 384, 841, 2207, 3120, 3485, 3678, 3701
SspI AATATT 4 cut(s) 499, 2038, 2396, 2587
SspMI CTAG 7 cut(s) 63, 639, 780, 1059, 1406, 2384, 3212
StuI AGGCCT 1 cut(s) 631
StyD4I CCNGG 2 cut(s) 427, 2472
StyI CCWWGG 3 cut(s) 207, 1249, 1415
TatI WGTACW 1 cut(s) 492
TauI GCSGC 2 cut(s) 275, 3680
TscAI CASTG 4 cut(s) 175, 867, 1324, 3084
TseFI GTSAC 3 cut(s) 1727, 1931, 3221
Tsp45I GTSAC 3 cut(s) 1727, 1931, 3221
TspGWI ACGGA 2 cut(s) 580, 1102
TspRI CASTG 4 cut(s) 175, 867, 1324, 3084
Van91I CCANNNNNTGG 1 cut(s) 1846
VneI GTGCAC 1 cut(s) 3259
VpaK11BI GGWCC 2 cut(s) 1289, 3142
VspI ATTAAT 2 cut(s) 2034, 3471
XceI RCATGY 2 cut(s) 2008, 3357
XcmI CCANNNNNNNNNTGG 2 cut(s) 205, 1847
XhoI CTCGAG 1 cut(s) 2109
XmiI GTMKAC 6 cut(s) 1038, 2217, 3127, 3168, 3502, 3558
XmnI GAANNNNTTC 3 cut(s) 9, 1561, 3654
XspI CTAG 7 cut(s) 63, 639, 780, 1059, 1406, 2384, 3212
ZraI GACGTC 1 cut(s) 3221
Zsp2I ATGCAT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.