Rw3G006430

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0007319
Physical Location & Seq
Forward (+)
6076720 .. 6086918
10199 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G006430.1

Sequence Viewer

Length: 3828 bp
ATGGAAAGGGTTCAGGTTCAGATCCCAGATACTCCTGCAAAGAAAACTCAGAGACAACGTTCTAGCGGGATTTCTATGCTTACTGCCCCTACAAATTCTTTAGAGGAAAACAAAGAGAACCCGATTGCAGAGGACTTGTCTGCAATGGAGGACGAGGTAGTGGATTGCAGTGGACTTATGGACACGATTTTGGATTCCATTCGTTCCTTGGATTTGAACAAGCCTGTGTGGGAGGAGTGGGAGACTGAGGAATTTGCTTATGGTTATAGGGCCGCATTATTGCAAACAGAAGATCTTGCAAGGGAGGATGAGGAAGTGTTGACATATAATCTTCCATGTTCTCAGTCATCTTCCAATACCGAAAATCCTGAAAATCACAGTTGCGGTTTTGGCAGCATAGAGGGTTCTTTCACGCCACTTGTGCAACCTGGTTATGCTGCTGAATCCATGTCACAATACAATTTGAAGAATCAGGAATTAGTTGATGGCAAGTACAATATTGCTGATACAAATTATATTGGTAGCTTAAAAAGAAAACTACATGCTGCTACACTTCAAATGCCACGGAAGAGACTCAAGAGGCCGAAGAAAAAAGTGTACCGGCCAAAAGTGGTACGTAATTTAGGAAGGCCTATACCTAGAATGGAACCCAAGACTCCCACCAAAAAGAATGAAAGGATGAAGCTCAAAACACCAAAAATGAAGAAAAATGTGTCATTGAAGAAGTCTTCGAGCACAATCAACCTCAACCATGTCCGAGTTCCTTTAGATTTGGAGCCTATTGTATCAGAGGGAGAATTTTCAAGCATTGACATAGAAAAGCACTCGAACGATGGAACCCGCATCATATCAGAGAAGACAGTGGAGCATTGCATCACAAAATGCATTGATTTAGGATTAAAATTTTTGACGAAGAAAAAGAGGACGACGATGGGAAAGAAATGGGAAGTGAAAGACTATAGTAGAATAATGGAACCGAATCTTCTGAAAATAAAAAAGAAAAGAAGGATGGGCAGGAGGAGATGGGAAGTAGACGTGTGGGAAAAAATAGCTAGCAAATTTCAGACAAACTATCTTAAACGGAGACGCAATAAAAAGAGAAGAGCTGCCCCCGAAATCAGTCAACAGGAAGTGCGCAACATCTTTAACACCGAAAAGCTGTTGTCTCCATGCTTCATCACCGAATCAGGAGTATTGAACATTGAAGAAAGCTTAACAGGAATGGTGAGCTTACTGGAAAGTGATAGTTTTGAGTTGTTCTTCAAATCAACAGATATACCTGAAGATAATATCAATGTTGTTGTGCAAAAACATCAACTTAACCATGGGATACTTGTTTGTTACCAAGAAAATGAGAAACATGGTCCTCACGTTGAGCTGGGTATCGTAGCCAGTGCAAATGGTGAGCTTGGTATGGTAGCCACGACAAAGGGAAAGGGAAGGAGGCAAGAAAAGCTTGAGAAATTAGAACATGGTGAACTAGCTCACCCATGGAAGCTTCAATTGCAAAATGAAGTTAAGGAGGGGGACAAACATGACCCGAACTCTGAATTTTGGGATAAGGAGAGACAATTCTTTCAGGGTCGGATCAACTTATTTTTAACTCGTATGCGCATAGTTCAAGGAAATAGAAAATTTTCACAATGGGATGGATCAGTTATGGATTCGATAGTCGGAGTTTTTTTAACACAGAACGTATCAGATCATCTATCCAGTTCTGCCTTTATATCACTCGCTGCAGCTTTCCCATATCAGCCCAGTTGTAACCAAGAATTAACTTATGCAAACTCAGAGCCAAGTTGTGAGCAATCAAGAATTGTTAAAATTGAGGAATTAGTAGAGGGTGGAATCTCAAAGTCCAGCTGTGAACAATCAAATGACCAAACATATTGTGAAGATATAAATAGTGGCAGCCATAAATTGGTTGGAAGTAACACGGTATTTGATAAAGAGTCGTTCACACAAGAAGCCACCACTACAATGAAGCACGAAAATCAGAAGTCAAGTGATTGCGAAATGGTATTAGCCAAACAAGTTGAGTTGCAGCAAAATTTTGAGGGTCAAGATTATTACGTGGACATGCCATCTGATAAGCCTGACTGCTCGATTGATATTATTACTGAAAATTCGCTTTCTTACTTCAATGAGGAAAAGCAAAGAGGAAAGAACTTGAAGGTGGATGCTCGAGAAATAAATGATGAGGTTGCTATGGTGAATGGAAAACCTGATTGGGATTATTATAGGAGAGAAATTGAAAAGGAAAGAAAGATCAGGCACATGGCGGATTCGGTAGACTGGCAGGCCGTTAGACAAGCAGATGTTGGTGATGTTGCTGAAGCCATCAAAGGACGTGGACAACATCGTGTCATCGCACATCGAATAAAGAAATTTCTTAATGACGTGAATCAAGACCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCAGACAAACTAGCAAGGAAATATTTGACTAATATCGAGGGAATTGGCCTGAAAAGTGTGGAGTGCGTGCGCCTTTTAGCACTGAAGCAGATAGCCTTCCCGGTGGACGTTAATGTTGGCAGGATAGCCATTCGTTTAGGGTGGGTGCCTATGCAACCATTACCTGAAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAAAAGTATCTTTGGCCTCGCCTAATGAAATTGACGTTCGAAGAATTGTATGAACTACACTATCAAATGATAACCTTTGGAAAGGTTTATTGTACCAAACGTAAACCTAATTGTCATTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTTAGTGCATATGCAAGTTCAAAATATGCTGCTTTACCGAAGTCAATGAAGAAGCAAGGACAAAAGAAAACTGATGTGGTTATTTGCAATAATCATCTTTCTTCTGGGTATGAGTCAGTAAATGCAGCATCACAATCATATCTTGAGGACACTACTAAATTAGAATATCAAATAAAAGATTGTGAACCGATAATTGAAGAACCAGCATCACCTCGACCGTCATTAGCGGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGATGATCCTTTTAAAGAGTTCGTAACAAGAAGGCCATATATGAATACATTTCGAGGAAGTGATATCTCAACACTAGTTCCCTTTATGACCCCAGAAGAAGCTACTTTCATCCCCGCTCGTAGACTAAAAGAAGTGGACCGTTTGAGGACAAAACACTCAGTCTACGTGATTAAAGATCAAGGCCCTTTATTTAAAGAGCTACAACTAGAGAGACGTCACCCTCATGATAATAGTCCTTACCTTTTTGCCTTGTGCATATCAGTATCTCAATCACCTGATCAAAACAGATGCAGTTGCAAGGGATCTAATTATTCATCATGTGATGGTGAGAAGACATGTTTTTCTTGTAACAGTCCTTCAACACAAGCTAATACTGTTCAAGGAACAATTTTGATACCTTGTAGAACAGCAATGAGGGGAAGTTTTCCATTAAACGGTACATATTTTCAAATTAATGAGGTTTTTGCGGATCATGAATCCAGTATACGCCCCATCAACGTTCATAGAGATTTGTTATGGGATCAGACGAGAAGAGATGTATACTTTGGAACATCAACAAGTGCAATTCTTAAAGGCTTATCAACATATGACGTTGCGATGTGTTTTAAGGAAGGTTCTATTTGCGTTAGAGCATTTAATCGGGAAACACGCAGGCCTGAGCCTCTATTAGAAAGGTTTCACATTTCAACCGTCAAAACCGCAAAGAAAGGAAAGAAAAAAGCGGAACAGAAAGAAGACGACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1275

Amino Acids

146.4

Weight (kDa)

8.03

Isoelectric Point (pI)

49.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1156 - 1256 2.5e-46 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 3301
Acc16I TGCGCA 2 cut(s) 1136, 1613
AccB1I GGYRCC 1 cut(s) 2593
AccB7I CCANNNNNTGG 1 cut(s) 1921
AccBSI CCGCTC 1 cut(s) 3200
AccI GTMKAC 6 cut(s) 1032, 2292, 3205, 3246, 3568, 3624
AclI AACGTT 2 cut(s) 58, 3582
AclWI GGATC 7 cut(s) 16, 1595, 1660, 3083, 3394, 3561, 3612
AcoI YGGCCR 1 cut(s) 602
AcuI CTGAAG 4 cut(s) 1302, 2355, 2552, 2634
AcyI GRCGYC 1 cut(s) 3298
AfaI GTAC 5 cut(s) 494, 599, 615, 2758, 3523
AfiI CCNNNNNNNGG 2 cut(s) 1921, 3518
AflIII ACRYGT 3 cut(s) 1035, 2629, 3419
AhlI ACTAGT 1 cut(s) 3157
AjiI CACGTC 3 cut(s) 1036, 2351, 2401
AjnI CCWGG 1 cut(s) 427
AjuI GAANNNNNNNTTGG 2 cut(s) 2212, 2244
Alw21I GWGCWC 1 cut(s) 737
Alw26I GTCTC 8 cut(s) 46, 236, 565, 1078, 1170, 1561, 3057, 3289
AlwI GGATC 7 cut(s) 16, 1595, 1660, 3083, 3394, 3561, 3612
Ama87I CYCGRG 1 cut(s) 2184
AseI ATTAAT 1 cut(s) 3537
Asp700I GAANNNNTTC 4 cut(s) 9, 1636, 2619, 3759
AspLEI GCGC 3 cut(s) 1137, 1614, 2520
AspS9I GGNCC 4 cut(s) 270, 1364, 3220, 3266
AsuC2I CCSGG 1 cut(s) 2549
AsuII TTCGAA 1 cut(s) 2703
AsuNHI GCTAGC 1 cut(s) 1052
AvaI CYCGRG 1 cut(s) 2184
AvaII GGWCC 2 cut(s) 1364, 3220
BanI GGYRCC 1 cut(s) 2593
BanII GRGCYC 1 cut(s) 2449
BarI GAAGNNNNNNTAC 2 cut(s) 3424, 3456
BbsI GAAGAC 3 cut(s) 720, 863, 3422
Bbv12I GWGCWC 1 cut(s) 737
BceAI ACGGC 1 cut(s) 2288
BciT130I CCWGG 1 cut(s) 429
BciVI GTATCC 1 cut(s) 1323
BclI TGATCA 1 cut(s) 3361
BcnI CCSGG 1 cut(s) 2549
BcoDI GTCTC 8 cut(s) 46, 236, 565, 1078, 1170, 1561, 3057, 3289
BcuI ACTAGT 1 cut(s) 3157
BfaI CTAG 7 cut(s) 63, 639, 1053, 1481, 2459, 3158, 3290
BfmI CTRYAG 2 cut(s) 958, 1737
BfuI GTATCC 1 cut(s) 1323
BglII AGATCT 1 cut(s) 292
Bme1390I CCNGG 2 cut(s) 429, 2549
Bme18I GGWCC 2 cut(s) 1364, 3220
BmeT110I CYCGRG 1 cut(s) 2184
BmgBI CACGTC 3 cut(s) 1036, 2351, 2401
BmgT120I GGNCC 4 cut(s) 270, 1364, 3220, 3266
BmiI GGNNCC 7 cut(s) 648, 777, 838, 975, 2448, 2595, 3045
BmrFI CCNGG 2 cut(s) 429, 2549
BmrI ACTGGG 1 cut(s) 1752
BmsI GCATC 7 cut(s) 852, 882, 2170, 2634, 2951, 3029, 3362
BmtI GCTAGC 1 cut(s) 1056
BmuI ACTGGG 1 cut(s) 1752
BpiI GAAGAC 3 cut(s) 720, 863, 3422
BpmI CTGGAG 1 cut(s) 2433
Bpu10I CCTNAGC 1 cut(s) 3741
Bpu14I TTCGAA 1 cut(s) 2703
BpuEI CTTGAG 4 cut(s) 560, 1478, 2450, 2978
BpuMI CCSGG 1 cut(s) 2549
BsaAI YACGTR 4 cut(s) 617, 2074, 2632, 3250
BsaHI GRCGYC 1 cut(s) 3298
BsaJI CCNNGG 4 cut(s) 207, 563, 1324, 1490
Bsc4I CCNNNNNNNGG 2 cut(s) 1921, 3518
Bse118I RCCGGY 2 cut(s) 600, 2647
Bse1I ACTGG 6 cut(s) 1239, 1392, 1713, 1758, 2300, 3564
Bse3DI GCAATG 3 cut(s) 150, 868, 3501
BseBI CCWGG 1 cut(s) 429
BseDI CCNNGG 4 cut(s) 207, 563, 1324, 1490
BseGI GGATG 6 cut(s) 313, 684, 1014, 1654, 2185, 3192
BseLI CCNNNNNNNGG 2 cut(s) 1921, 3518
BseMI GCAATG 3 cut(s) 150, 868, 3501
BseMII CTCAG 6 cut(s) 62, 237, 356, 1803, 3255, 3732
BseNI ACTGG 6 cut(s) 1239, 1392, 1713, 1758, 2300, 3564
BseRI GAGGAG 2 cut(s) 248, 1033
BseYI CCCAGC 1 cut(s) 1378
Bsh1285I CGRYCG 1 cut(s) 3032
BshNI GGYRCC 1 cut(s) 2593
BsiEI CGRYCG 1 cut(s) 3032
BsiHKAI GWGCWC 1 cut(s) 737
BsiHKCI CYCGRG 1 cut(s) 2184
BsiSI CCGG 3 cut(s) 601, 2549, 2648
BslFI GGGAC 1 cut(s) 1541
BslI CCNNNNNNNGG 2 cut(s) 1921, 3518
BsmAI GTCTC 8 cut(s) 46, 236, 565, 1078, 1170, 1561, 3057, 3289
BsmBI CGTCTC 2 cut(s) 1078, 3289
BsmFI GGGAC 1 cut(s) 1541
BsoBI CYCGRG 1 cut(s) 2184
Bsp119I TTCGAA 1 cut(s) 2703
Bsp1286I GDGCHC 2 cut(s) 737, 2449
Bsp19I CCATGG 2 cut(s) 1324, 1490
BspCNI CTCAG 6 cut(s) 61, 238, 355, 1802, 3254, 3733
BspHI TCATGA 2 cut(s) 3307, 3556
BspLI GGNNCC 7 cut(s) 648, 777, 838, 975, 2448, 2595, 3045
BspMAI CTGCAG 1 cut(s) 1741
BspOI GCTAGC 1 cut(s) 1056
BspPI GGATC 7 cut(s) 16, 1595, 1660, 3083, 3394, 3561, 3612
BspQI GCTCTTC 1 cut(s) 1096
BspT104I TTCGAA 1 cut(s) 2703
BspT107I GGYRCC 1 cut(s) 2593
BsrBI CCGCTC 1 cut(s) 3200
BsrDI GCAATG 3 cut(s) 150, 868, 3501
BsrFI RCCGGY 2 cut(s) 600, 2647
BsrI ACTGG 6 cut(s) 1239, 1392, 1713, 1758, 2300, 3564
BssAI RCCGGY 2 cut(s) 600, 2647
BssECI CCNNGG 4 cut(s) 207, 563, 1324, 1490
BssNAI GTATAC 2 cut(s) 3569, 3625
BssNI GRCGYC 1 cut(s) 3298
BssT1I CCWWGG 3 cut(s) 207, 1324, 1490
Bst1107I GTATAC 2 cut(s) 3569, 3625
Bst2UI CCWGG 1 cut(s) 429
Bst4CI ACNGT 9 cut(s) 380, 862, 1939, 3033, 3224, 3437, 3460, 3521, 3775
Bst6I CTCTTC 4 cut(s) 563, 1096, 2634, 3610
BstACI GRCGYC 1 cut(s) 3298
BstBAI YACGTR 4 cut(s) 617, 2074, 2632, 3250
BstBI TTCGAA 1 cut(s) 2703
BstC8I GCNNGC 4 cut(s) 1054, 2301, 2516, 3737
BstDEI CTNAG 8 cut(s) 48, 246, 342, 1789, 2438, 2816, 3241, 3741
BstDSI CCRYGG 3 cut(s) 563, 1324, 1490
BstF5I GGATG 6 cut(s) 313, 684, 1014, 1654, 2185, 3192
BstHHI GCGC 3 cut(s) 1137, 1614, 2520
BstMAI GTCTC 8 cut(s) 46, 236, 565, 1078, 1170, 1561, 3057, 3289
BstMCI CGRYCG 1 cut(s) 3032
BstMWI GCNNNNNNNGC 4 cut(s) 390, 421, 1453, 1504
BstNI CCWGG 1 cut(s) 429
BstNSI RCATGY 3 cut(s) 545, 2083, 3423
BstSCI CCNGG 2 cut(s) 427, 2547
BstSFI CTRYAG 2 cut(s) 958, 1737
BstSNI TACGTA 1 cut(s) 617
BstV2I GAAGAC 3 cut(s) 720, 863, 3422
BstX2I RGATCY 3 cut(s) 21, 292, 3386
BstYI RGATCY 3 cut(s) 21, 292, 3386
BstZ17I GTATAC 2 cut(s) 3569, 3625
BsuI GTATCC 1 cut(s) 1323
BtgI CCRYGG 3 cut(s) 563, 1324, 1490
BtgZI GCGATG 2 cut(s) 2353, 3695
BtrI CACGTC 3 cut(s) 1036, 2351, 2401
BtsCI GGATG 6 cut(s) 313, 684, 1014, 1654, 2185, 3192
BtsI GCAGTG 1 cut(s) 175
BtsIMutI CAGTG 4 cut(s) 175, 867, 1399, 2528
Cac8I GCNNGC 4 cut(s) 1054, 2301, 2516, 3737
CciI TCATGA 2 cut(s) 3307, 3556
CfoI GCGC 3 cut(s) 1137, 1614, 2520
Cfr10I RCCGGY 2 cut(s) 600, 2647
Cfr13I GGNCC 4 cut(s) 270, 1364, 3220, 3266
CseI GACGC 1 cut(s) 1095
CsiI ACCWGGT 1 cut(s) 427
Csp6I GTAC 5 cut(s) 493, 598, 614, 2757, 3522
CspCI CAANNNNNGTGG 2 cut(s) 2332, 2367
CviQI GTAC 5 cut(s) 493, 598, 614, 2757, 3522
DdeI CTNAG 8 cut(s) 48, 246, 342, 1789, 2438, 2816, 3241, 3741
DraI TTTAAA 2 cut(s) 3097, 3277
EaeI YGGCCR 1 cut(s) 602
Eam1104I CTCTTC 4 cut(s) 563, 1096, 2634, 3610
EarI CTCTTC 4 cut(s) 563, 1096, 2634, 3610
EciI GGCGGA 1 cut(s) 2297
Eco105I TACGTA 1 cut(s) 617
Eco130I CCWWGG 3 cut(s) 207, 1324, 1490
Eco147I AGGCCT 2 cut(s) 631, 3739
Eco24I GRGCYC 1 cut(s) 2449
Eco32I GATATC 1 cut(s) 3148
Eco47I GGWCC 2 cut(s) 1364, 3220
Eco57I CTGAAG 4 cut(s) 1302, 2355, 2552, 2634
Eco88I CYCGRG 1 cut(s) 2184
EcoO109I RGGNCCY 1 cut(s) 3266
EcoRII CCWGG 1 cut(s) 427
EcoRV GATATC 1 cut(s) 3148
EcoT14I CCWWGG 3 cut(s) 207, 1324, 1490
EcoT22I ATGCAT 1 cut(s) 887
EcoT38I GRGCYC 1 cut(s) 2449
ErhI CCWWGG 3 cut(s) 207, 1324, 1490
Esp3I CGTCTC 2 cut(s) 1078, 3289
FalI AAGNNNNNCTT 2 cut(s) 1440, 1472
FaqI GGGAC 1 cut(s) 1541
FauI CCCGC 3 cut(s) 59, 848, 3205
FauNDI CATATG 2 cut(s) 2824, 3670
FbaI TGATCA 1 cut(s) 3361
FblI GTMKAC 6 cut(s) 1032, 2292, 3205, 3246, 3568, 3624
FokI GGATG 6 cut(s) 320, 691, 1021, 1661, 2192, 3179
FriOI GRGCYC 1 cut(s) 2449
FspAI RTGCGCAY 1 cut(s) 1613
FspBI CTAG 7 cut(s) 63, 639, 1053, 1481, 2459, 3158, 3290
FspI TGCGCA 2 cut(s) 1136, 1613
GlaI GCGC 3 cut(s) 1136, 1613, 2519
GsaI CCCAGC 1 cut(s) 1382
GsuI CTGGAG 1 cut(s) 2433
HapII CCGG 3 cut(s) 601, 2549, 2648
HgaI GACGC 1 cut(s) 1095
HhaI GCGC 3 cut(s) 1137, 1614, 2520
Hin1I GRCGYC 1 cut(s) 3298
Hin6I GCGC 3 cut(s) 1135, 1612, 2518
HinP1I GCGC 3 cut(s) 1135, 1612, 2518
HincII GTYRAC 2 cut(s) 321, 1124
HindII GTYRAC 2 cut(s) 321, 1124
HindIII AAGCTT 3 cut(s) 1210, 1454, 1496
HpaII CCGG 3 cut(s) 601, 2549, 2648
Hpy99I CGWCG 1 cut(s) 931
HpyAV CCTTC 8 cut(s) 621, 999, 1434, 2167, 2554, 3108, 3450, 3689
HpyCH4III ACNGT 9 cut(s) 380, 862, 1939, 3033, 3224, 3437, 3460, 3521, 3775
HpyF10VI GCNNNNNNNGC 4 cut(s) 390, 421, 1453, 1504
HpyF3I CTNAG 8 cut(s) 48, 246, 342, 1789, 2438, 2816, 3241, 3741
Hsp92I GRCGYC 1 cut(s) 3298
HspAI GCGC 3 cut(s) 1135, 1612, 2518
Ksp22I TGATCA 1 cut(s) 3361
LguI GCTCTTC 1 cut(s) 1096
LmnI GCTCC 3 cut(s) 775, 865, 2452
LweI GCATC 7 cut(s) 852, 882, 2170, 2634, 2951, 3029, 3362
MabI ACCWGGT 1 cut(s) 427
MaeI CTAG 7 cut(s) 63, 639, 1053, 1481, 2459, 3158, 3290
MaeIII GTNAC 7 cut(s) 450, 1340, 1763, 1931, 3106, 3299, 3431
MbiI CCGCTC 1 cut(s) 3200
MfeI CAATTG 1 cut(s) 1502
MflI RGATCY 3 cut(s) 21, 292, 3386
MhlI GDGCHC 2 cut(s) 737, 2449
MlyI GAGTC 4 cut(s) 567, 649, 1961, 2936
MmeI TCCRAC 3 cut(s) 1565, 1654, 1906
Mph1103I ATGCAT 1 cut(s) 887
MroXI GAANNNNTTC 4 cut(s) 9, 1636, 2619, 3759
MslI CAYNNNNRTG 2 cut(s) 1979, 3306
MspA1I CMGCKG 1 cut(s) 1863
MspI CCGG 3 cut(s) 601, 2549, 2648
MspR9I CCNGG 2 cut(s) 429, 2549
MunI CAATTG 1 cut(s) 1502
MvaI CCWGG 1 cut(s) 429
MwoI GCNNNNNNNGC 4 cut(s) 390, 421, 1453, 1504
NciI CCSGG 1 cut(s) 2549
NcoI CCATGG 2 cut(s) 1324, 1490
NdeI CATATG 2 cut(s) 2824, 3670
NheI GCTAGC 1 cut(s) 1052
NlaIV GGNNCC 7 cut(s) 648, 777, 838, 975, 2448, 2595, 3045
NmuCI GTSAC 2 cut(s) 450, 3299
NsbI TGCGCA 2 cut(s) 1136, 1613
NsiI ATGCAT 1 cut(s) 887
NspI RCATGY 3 cut(s) 545, 2083, 3423
NspV TTCGAA 1 cut(s) 2703
PaeR7I CTCGAG 1 cut(s) 2184
PagI TCATGA 2 cut(s) 3307, 3556
PceI AGGCCT 2 cut(s) 631, 3739
PciI ACATGT 1 cut(s) 3419
PciSI GCTCTTC 1 cut(s) 1096
PdmI GAANNNNTTC 4 cut(s) 9, 1636, 2619, 3759
PflMI CCANNNNNTGG 1 cut(s) 1921
PleI GAGTC 4 cut(s) 567, 649, 1960, 2935
PpsI GAGTC 4 cut(s) 567, 649, 1960, 2935
Ppu21I YACGTR 4 cut(s) 617, 2074, 2632, 3250
PscI ACATGT 1 cut(s) 3419
PshBI ATTAAT 1 cut(s) 3537
Psp1406I AACGTT 2 cut(s) 58, 3582
Psp6I CCWGG 1 cut(s) 427
PspFI CCCAGC 1 cut(s) 1378
PspGI CCWGG 1 cut(s) 427
PspN4I GGNNCC 7 cut(s) 648, 777, 838, 975, 2448, 2595, 3045
PspPI GGNCC 4 cut(s) 270, 1364, 3220, 3266
PstI CTGCAG 1 cut(s) 1741
PsuI RGATCY 3 cut(s) 21, 292, 3386
PvuII CAGCTG 1 cut(s) 1863
RsaI GTAC 5 cut(s) 494, 599, 615, 2758, 3523
RsaNI GTAC 5 cut(s) 493, 598, 614, 2757, 3522
RseI CAYNNNNRTG 2 cut(s) 1979, 3306
SapI GCTCTTC 1 cut(s) 1096
Sau96I GGNCC 4 cut(s) 270, 1364, 3220, 3266
SchI GAGTC 4 cut(s) 567, 649, 1961, 2936
ScrFI CCNGG 2 cut(s) 429, 2549
SduI GDGCHC 2 cut(s) 737, 2449
SexAI ACCWGGT 1 cut(s) 427
SfaNI GCATC 7 cut(s) 852, 882, 2170, 2634, 2951, 3029, 3362
SfcI CTRYAG 2 cut(s) 958, 1737
Sfr274I CTCGAG 1 cut(s) 2184
SfuI TTCGAA 1 cut(s) 2703
SinI GGWCC 2 cut(s) 1364, 3220
SlaI CTCGAG 1 cut(s) 2184
SmiMI CAYNNNNRTG 2 cut(s) 1979, 3306
SmlI CTYRAG 5 cut(s) 575, 1457, 2184, 2429, 2957
SmoI CTYRAG 5 cut(s) 575, 1457, 2184, 2429, 2957
SnaBI TACGTA 1 cut(s) 617
SpeI ACTAGT 1 cut(s) 3157
SseBI AGGCCT 2 cut(s) 631, 3739
SspI AATATT 3 cut(s) 499, 2471, 2662
SspMI CTAG 7 cut(s) 63, 639, 1053, 1481, 2459, 3158, 3290
StuI AGGCCT 2 cut(s) 631, 3739
StyD4I CCNGG 2 cut(s) 427, 2547
StyI CCWWGG 3 cut(s) 207, 1324, 1490
TaaI ACNGT 9 cut(s) 380, 862, 1939, 3033, 3224, 3437, 3460, 3521, 3775
TatI WGTACW 1 cut(s) 492
TauI GCSGC 1 cut(s) 275
TscAI CASTG 4 cut(s) 175, 867, 1399, 2535
TseFI GTSAC 2 cut(s) 450, 3299
Tsp45I GTSAC 2 cut(s) 450, 3299
TspGWI ACGGA 2 cut(s) 580, 1096
TspRI CASTG 4 cut(s) 175, 867, 1399, 2535
Van91I CCANNNNNTGG 1 cut(s) 1921
VpaK11BI GGWCC 2 cut(s) 1364, 3220
VspI ATTAAT 1 cut(s) 3537
XceI RCATGY 3 cut(s) 545, 2083, 3423
XcmI CCANNNNNNNNNTGG 2 cut(s) 205, 1922
XhoI CTCGAG 1 cut(s) 2184
XmiI GTMKAC 6 cut(s) 1032, 2292, 3205, 3246, 3568, 3624
XmnI GAANNNNTTC 4 cut(s) 9, 1636, 2619, 3759
XspI CTAG 7 cut(s) 63, 639, 1053, 1481, 2459, 3158, 3290
ZraI GACGTC 1 cut(s) 3299
Zsp2I ATGCAT 1 cut(s) 887
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.