Rh3AG075100

FES

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3A
OG0007319
Physical Location & Seq
Forward (+)
6027922 .. 6038335
10414 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3AG075100.1

Sequence Viewer

Length: 3888 bp
ATGGCTTTCCAACTCTTCGAAACAAACCACAAATCAACAGTAGTACAGAACATGATGGCACCGATGTCAAAAGTTTACGAGAGGCAGAAGAGACAGCACGTTGGGTCTGAAGACAACAGTGTCATGGAACGGGTTCAGGTTCAGATCCCAGATACTCCTGCAAAGAAAACTCAGAGACAACGTTCTAGCGGGATTTCTATGCTTACTGCCCCTACAAATTCTTTGGAAAACAAAGAGAACCCGATTGCAGAGGACTTGTCTGCAATGGACGACGAGGTAGTGGATTGCAGTGGACTTATGGACACGATTTTGGATTCCATTCGTTCCTTGGATTTGAACAAGCCTGTGTGGGAGGAGTGGGAGACTGAGGAATTTGCTTATGGTTATAGGGCCGCATTATTGCAAACAGAAGATCTTGCAAGGGATGATGAGGAAGTGTTGACATATAATCTTCCATGTTCTCAGTCATCTTCCAATACCGAAAATCCTGAAAATCACAGTTGCGGTTTTGGCAGCATAGAGGATTCTTTCATGCCAGTTGTGCAACCTGGTTATGCTGCTGAATCCATGTCACAATATAATTTGAAGAATCAGGAATTAGTTGATGGCAAGTACAATATTGCTGATACAAATTATATTGGTAGCTTAAAAAGAAAACTACATGCTGCTACACTTCAAATGCCACGGAAGAGACTCAAGAGGCCGAAGAAAAAAGTTTACCGGCCAAAAGTGGTACGTAATTTAGGAAGGCCTATACCTAGAATGGAACCCAAGACTCCCACCAAAAAGAATGAAAGGATGAAGCTCAAAACACCAAAAATGAAGAAAAATGTGTCATTGAAGAAGTCTTCGAGCACAATCAACCTCAACCATGTCCGAGTTCCTTTAGATTTGGAGCCTATTGTATCAGAGGGAGAATTTTCAAGCATTGACATAGAAAAGCACTCTAACGATGGAACCCGCATCATATCAGAGAAGACAGTTGAGCATTGCATCACAAAATGCATTGATTTAGGATTAAATTTTTTGACGAAGAAAAAGAGGACGACGATGGGAAAGAAATGGGAAGTGAAAGACTATAGTAGAATAATGGAACCGAATCTTCTGAAAATAAAAAAGAAAAAAAGGATGGGCAGGAGGAGATGGGAAGTAGACGTGTGGAAAAAAATAGCTAGCAAGTTTCAGACAAACTATCTTAAACGGAGACGCAATAAAAAGAGAAGAGCTGCCCCCGAAATCAGTCAACAGGAAGTGCGCAACATCTTTAACACCGAAAAGCTGTTGTCTCCATGCTTCATCACCGAATCAGGAGTATTGAACATTGAAGAAAGCTTAACAGGAATGGTGAGCTTACTGGAAAGTGATAGTTTTGAGTTGTTCTTCAAATCAACAGATATACCTGAAGATAATATCAATGTTGTTGTGCAAAAACATCAACTTAACCATGATATACTTGTTTGTTACCAAGAAAATGAGAAACATGGTCCTCACGTTGAGCTGGGTATCGTAGCCAGTGCAAATGGTGAGCTTGGTATGGTAGCCACGACAAAGGGAAAGGGAAGGAGGCAAGAAAAGCTTGAGAAATTAGAACATGGTGAACTAGCTCACCCATGGAAGCTTCAATTGCAAAATGAAGTTAAGGAGGGGGACAAACATGACCCGAACTCTGAATTTTGGGATAAGGAGAGACAATTCTTTCAGGGTCGGATCAACTTATTTTTAACTCGTATGCGCATAGTTCAAGGAAATAGAAAATTTTCAGAATGGGATGGATCAGTTATGGATTCGATAGTCGGAGTTTTTTTAACACAGAACGTATCAGATCATCTATCCAGTTCTGCCTTTATATCACTCGCTGCAGCTTTCCCATATCAGCCCAGTTGTAACCAAGAATTGACTTATGCAAACTCAGAGCCAAGTTGTGAGCAATCAAGAATTGTTAAAATTGAGGAATTAGTAGAGGGTGGAATCTCAAAGTCCAGCTGTGAACAATCAAATGACCAAACATATTGTGAAGATATAAATAGTGGCAGCCATAAATTGGTTGGAAGATATAAAGAGTCGTTCACACAAGAAGCCACAACTACAATGAAGTACGAAAATCAGAAGTCAAGTGATTGTGAAATGGTATTAGCCAAACACGTTGAGTTGCAGCAAAATTTTGAGGGTCAAGATTATTACGTGGACATGCCATCTGATAAGCCTGACTGCTCGATTGATATTATTACTGAAAATTCGCTTTCTTACTTCAATGAGGAAAAGCAAAGAGGAAAGAAGTTGAAGGTGGATGCTCGAGAAATAAATAATGAGGTTGCTATGGTGAATGGAAAACCTGATTGGGATTATTATAGGAGAGAAATTGAAAAGGAAAGAAAGATCAGGCACATGGCGGATTCGGTAGACTGGCAGGCCGTTAGACAAGCAGATGTTGGTGATGTTGCTGAAGCCATCAAAGGACGTGGACAACATCGTGTCATCGCAGATCGAATAAAGAAATTTCTTAATGACGTGAATCAAGACCATAAAGAAATAAACCTTGAGTGGCTAAGATGGGCTCCAGACAAACTAGCAAGGAAATATTTGACTAATATCGAGGGAATTGGCCTGAAAAGTGTGGAGTGCGTGCGCCTTTTAGCACTCAAGCAGATAGCCTTCCCGGTGGACGTTAATGTTGGCAGGATAGCCATTCGTTTAGGGTGGGTGCCTATGCAACCATTACCTGAAGATGTTCAGATACACGTATTAGAAGAGATGCCGGTTATGGACAATATTCAAAAGTATCTTTGGCCTCGGCTAATGAAATTGAAGTTCGAAGAATTGTATGAACTACACTATCAAATGATAACCTTTGGAAAGGTTTATTGTACCAAACGTAAACCTAATTGTCATTCATGTCCAATGAAGAACGAGTGCAAGTATTATCTTAGTGCATATGCAAGTTCAAAATATGCTGCTTTACCGAAGTCAATGAAAAAGCAAGGACAAAAGAAAACTGATGTGGTTATTTGCAATAATCATCTTTCTTCTGGGTATGAGTCAGTAAATGCAGCATCACAATCATATCTTGAGGACACTACTAAATTAGAATATCAAATAAAAGATTGTGAACCGATAATTGAAGAACCAGCATCACCTCGACCGTCATTAGCGGAACCAGAATATGTGGAAAGAGACATTGAAGATTTATTCCTTGATGATCCTTTTAAAGAGTTCGTAACAAGAAGTCCATATATGAATACATTTCGAGGAAGTGATATCTCAACACTAGTTCCCTTTATGACCCCAGAAGAAGCTGCTTTCATCCCCGCTCGTAGACTAAAAGAAGTGGACCGTTTGAGGACAAAACACTCGGTCTACGTGATTAAAGATCAAGGCCCTTTATTTAAAGAGCTACAACTAGAGAGACGTCACCCTCATGATAATAGTCCTTACCTTTTTGCCTTGTGCACATCAGATCAAATGGAAAAGGTATCTCAATCACCTGATCAAAACAGATGCAGTTGCAAGGGATCTAATGATTCATCATGTGATGGTGAGAAGACATGTTTTTCTTGTAACAGTCTTTCAACACAAGCTAATACTGTTCAAGGAACAATTTTGATACCTTGTAGAACAGCAATGAGGGGAAGATTTCCATTAAACGGTACATATTTTCAAACTAATGAGGTTTTTGCGGATCATGAATCCAGTATACGCCCCATCAACGTTCACAGAGATTTGTTATGGGATCAGACGAGAAGAGATGTATACTTTGGAACATCAACAAGTGCAATTCTTAAAGGCTTATCAACATATGACGTTGCGATGTGTTTTAAGGAAGGTTCTATTTGCGTTAGAGCATTTAATCGGGAAACACGCAGGCCTGAGCCTCTATTAGAAAGTTTCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Perm-CXXC RRM_DME

Protein Analysis

1295

Amino Acids

148.94

Weight (kDa)

7.8

Isoelectric Point (pI)

48.63

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
RRM_DME PF15628 1197 - 1293 5.6e-44 RRM in Demeter
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 119
AatII GACGTC 1 cut(s) 3409
Acc16I TGCGCA 2 cut(s) 1256, 1733
AccB1I GGYRCC 2 cut(s) 58, 2701
AccB7I CCANNNNNTGG 1 cut(s) 2041
AccBSI CCGCTC 1 cut(s) 3308
AccI GTMKAC 6 cut(s) 1152, 2400, 3313, 3354, 3691, 3747
AciI CCGC 8 cut(s) 189, 393, 504, 961, 2390, 3149, 3306, 3674
AclI AACGTT 2 cut(s) 181, 3705
AclWI GGATC 7 cut(s) 139, 1715, 1780, 3191, 3517, 3684, 3735
AcoI YGGCCR 1 cut(s) 722
AcsI RAATTY 9 cut(s) 217, 371, 917, 1021, 1670, 1754, 2158, 2233, 2495
AcuI CTGAAG 4 cut(s) 129, 1422, 2463, 2742
AcyI GRCGYC 1 cut(s) 3406
AfaI GTAC 6 cut(s) 45, 614, 735, 2096, 2866, 3646
AfiI CCNNNNNNNGG 2 cut(s) 2041, 3641
AflIII ACRYGT 4 cut(s) 1155, 2140, 2737, 3542
AhlI ACTAGT 1 cut(s) 3265
AjiI CACGTC 3 cut(s) 1156, 2459, 2509
AjnI CCWGG 1 cut(s) 547
AjuI GAANNNNNNNTTGG 2 cut(s) 2320, 2352
Alw21I GWGCWC 2 cut(s) 857, 3449
Alw26I GTCTC 9 cut(s) 85, 169, 356, 685, 1198, 1290, 1681, 3165, 3397
Alw44I GTGCAC 1 cut(s) 3445
AlwI GGATC 7 cut(s) 139, 1715, 1780, 3191, 3517, 3684, 3735
Ama87I CYCGRG 1 cut(s) 2292
AoxI GGCC 9 cut(s) 390, 701, 722, 749, 2409, 2602, 2786, 3373, 3860
ApaLI GTGCAC 1 cut(s) 3445
ApoI RAATTY 9 cut(s) 217, 371, 917, 1021, 1670, 1754, 2158, 2233, 2495
Asp700I GAANNNNTTC 3 cut(s) 132, 1756, 2727
AspLEI GCGC 3 cut(s) 1257, 1734, 2628
AspS9I GGNCC 4 cut(s) 390, 1484, 3328, 3374
AsuC2I CCSGG 1 cut(s) 2657
AsuII TTCGAA 2 cut(s) 18, 2811
AsuNHI GCTAGC 1 cut(s) 1172
AvaI CYCGRG 1 cut(s) 2292
AvaII GGWCC 2 cut(s) 1484, 3328
BaeGI GKGCMC 1 cut(s) 3449
BanI GGYRCC 2 cut(s) 58, 2701
BanII GRGCYC 1 cut(s) 2557
BbsI GAAGAC 4 cut(s) 117, 840, 983, 3545
Bbv12I GWGCWC 2 cut(s) 857, 3449
BceAI ACGGC 1 cut(s) 2396
BciT130I CCWGG 1 cut(s) 549
BclI TGATCA 1 cut(s) 3484
BcnI CCSGG 1 cut(s) 2657
BcoDI GTCTC 9 cut(s) 85, 169, 356, 685, 1198, 1290, 1681, 3165, 3397
BcuI ACTAGT 1 cut(s) 3265
BfaI CTAG 7 cut(s) 186, 759, 1173, 1601, 2567, 3266, 3398
BfmI CTRYAG 2 cut(s) 1078, 1857
BglII AGATCT 1 cut(s) 412
Bme1390I CCNGG 2 cut(s) 549, 2657
Bme18I GGWCC 2 cut(s) 1484, 3328
BmeT110I CYCGRG 1 cut(s) 2292
BmgBI CACGTC 3 cut(s) 1156, 2459, 2509
BmgT120I GGNCC 4 cut(s) 390, 1484, 3328, 3374
BmiI GGNNCC 8 cut(s) 60, 768, 897, 958, 1095, 2556, 2703, 3153
BmrFI CCNGG 2 cut(s) 549, 2657
BmrI ACTGGG 1 cut(s) 1872
BmsI GCATC 7 cut(s) 972, 1002, 2278, 2742, 3059, 3137, 3485
BmtI GCTAGC 1 cut(s) 1176
BmuI ACTGGG 1 cut(s) 1872
BpiI GAAGAC 4 cut(s) 117, 840, 983, 3545
BpmI CTGGAG 1 cut(s) 2541
Bpu10I CCTNAGC 1 cut(s) 3864
Bpu14I TTCGAA 2 cut(s) 18, 2811
BpuEI CTTGAG 5 cut(s) 680, 1598, 2558, 2624, 3086
BpuMI CCSGG 1 cut(s) 2657
BsaAI YACGTR 4 cut(s) 737, 2182, 2740, 3358
BsaHI GRCGYC 1 cut(s) 3406
BsaJI CCNNGG 4 cut(s) 327, 683, 1610, 2789
Bsc4I CCNNNNNNNGG 2 cut(s) 2041, 3641
Bse118I RCCGGY 2 cut(s) 720, 2755
Bse1I ACTGG 7 cut(s) 536, 1359, 1512, 1833, 1878, 2408, 3687
Bse3DI GCAATG 3 cut(s) 270, 988, 3624
BseBI CCWGG 1 cut(s) 549
BseDI CCNNGG 4 cut(s) 327, 683, 1610, 2789
BseGI GGATG 6 cut(s) 430, 804, 1134, 1774, 2293, 3300
BseLI CCNNNNNNNGG 2 cut(s) 2041, 3641
BseMI GCAATG 3 cut(s) 270, 988, 3624
BseMII CTCAG 5 cut(s) 185, 357, 476, 1923, 3855
BseNI ACTGG 7 cut(s) 536, 1359, 1512, 1833, 1878, 2408, 3687
BseRI GAGGAG 2 cut(s) 368, 1153
BseSI GKGCMC 1 cut(s) 3449
BseYI CCCAGC 1 cut(s) 1498
Bsh1285I CGRYCG 1 cut(s) 3140
BshFI GGCC 9 cut(s) 392, 703, 724, 751, 2411, 2604, 2788, 3375, 3862
BshNI GGYRCC 2 cut(s) 58, 2701
BsiEI CGRYCG 1 cut(s) 3140
BsiHKAI GWGCWC 2 cut(s) 857, 3449
BsiHKCI CYCGRG 1 cut(s) 2292
BsiSI CCGG 3 cut(s) 721, 2657, 2756
BslFI GGGAC 1 cut(s) 1661
BslI CCNNNNNNNGG 2 cut(s) 2041, 3641
BsmAI GTCTC 9 cut(s) 85, 169, 356, 685, 1198, 1290, 1681, 3165, 3397
BsmBI CGTCTC 2 cut(s) 1198, 3397
BsmFI GGGAC 1 cut(s) 1661
BsnI GGCC 9 cut(s) 392, 703, 724, 751, 2411, 2604, 2788, 3375, 3862
BsoBI CYCGRG 1 cut(s) 2292
Bsp119I TTCGAA 2 cut(s) 18, 2811
Bsp1286I GDGCHC 3 cut(s) 857, 2557, 3449
Bsp19I CCATGG 1 cut(s) 1610
BspACI CCGC 8 cut(s) 189, 393, 504, 961, 2390, 3149, 3306, 3674
BspANI GGCC 9 cut(s) 392, 703, 724, 751, 2411, 2604, 2788, 3375, 3862
BspCNI CTCAG 5 cut(s) 184, 358, 475, 1922, 3856
BspHI TCATGA 2 cut(s) 3415, 3679
BspLI GGNNCC 8 cut(s) 60, 768, 897, 958, 1095, 2556, 2703, 3153
BspMAI CTGCAG 1 cut(s) 1861
BspOI GCTAGC 1 cut(s) 1176
BspPI GGATC 7 cut(s) 139, 1715, 1780, 3191, 3517, 3684, 3735
BspQI GCTCTTC 1 cut(s) 1216
BspT104I TTCGAA 2 cut(s) 18, 2811
BspT107I GGYRCC 2 cut(s) 58, 2701
BsrBI CCGCTC 1 cut(s) 3308
BsrDI GCAATG 3 cut(s) 270, 988, 3624
BsrFI RCCGGY 2 cut(s) 720, 2755
BsrI ACTGG 7 cut(s) 536, 1359, 1512, 1833, 1878, 2408, 3687
BssAI RCCGGY 2 cut(s) 720, 2755
BssECI CCNNGG 4 cut(s) 327, 683, 1610, 2789
BssNAI GTATAC 2 cut(s) 3692, 3748
BssNI GRCGYC 1 cut(s) 3406
BssT1I CCWWGG 2 cut(s) 327, 1610
Bst1107I GTATAC 2 cut(s) 3692, 3748
Bst2UI CCWGG 1 cut(s) 549
Bst4CI ACNGT 9 cut(s) 40, 119, 500, 982, 3141, 3332, 3560, 3583, 3644
Bst6I CTCTTC 6 cut(s) 20, 83, 683, 1216, 2742, 3733
BstACI GRCGYC 1 cut(s) 3406
BstBAI YACGTR 4 cut(s) 737, 2182, 2740, 3358
BstBI TTCGAA 2 cut(s) 18, 2811
BstC8I GCNNGC 4 cut(s) 1174, 2409, 2624, 3860
BstDEI CTNAG 7 cut(s) 171, 366, 462, 1909, 2546, 2924, 3864
BstDSI CCRYGG 2 cut(s) 683, 1610
BstF5I GGATG 6 cut(s) 430, 804, 1134, 1774, 2293, 3300
BstHHI GCGC 3 cut(s) 1257, 1734, 2628
BstMAI GTCTC 9 cut(s) 85, 169, 356, 685, 1198, 1290, 1681, 3165, 3397
BstMCI CGRYCG 1 cut(s) 3140
BstMWI GCNNNNNNNGC 4 cut(s) 510, 541, 1573, 1624
BstNI CCWGG 1 cut(s) 549
BstNSI RCATGY 3 cut(s) 665, 2191, 3546
BstSCI CCNGG 2 cut(s) 547, 2655
BstSFI CTRYAG 2 cut(s) 1078, 1857
BstSLI GKGCMC 1 cut(s) 3449
BstSNI TACGTA 1 cut(s) 737
BstV2I GAAGAC 4 cut(s) 117, 840, 983, 3545
BstX2I RGATCY 3 cut(s) 144, 412, 3509
BstYI RGATCY 3 cut(s) 144, 412, 3509
BstZ17I GTATAC 2 cut(s) 3692, 3748
BsuRI GGCC 9 cut(s) 392, 703, 724, 751, 2411, 2604, 2788, 3375, 3862
BtgI CCRYGG 2 cut(s) 683, 1610
BtgZI GCGATG 2 cut(s) 2461, 3818
BtrI CACGTC 3 cut(s) 1156, 2459, 2509
BtsCI GGATG 6 cut(s) 430, 804, 1134, 1774, 2293, 3300
BtsI GCAGTG 1 cut(s) 295
BtsIMutI CAGTG 3 cut(s) 124, 295, 1519
Cac8I GCNNGC 4 cut(s) 1174, 2409, 2624, 3860
CciI TCATGA 2 cut(s) 3415, 3679
CfoI GCGC 3 cut(s) 1257, 1734, 2628
Cfr10I RCCGGY 2 cut(s) 720, 2755
Cfr13I GGNCC 4 cut(s) 390, 1484, 3328, 3374
CseI GACGC 1 cut(s) 1215
CsiI ACCWGGT 1 cut(s) 547
Csp6I GTAC 6 cut(s) 44, 613, 734, 2095, 2865, 3645
CspCI CAANNNNNGTGG 2 cut(s) 2440, 2475
CviQI GTAC 6 cut(s) 44, 613, 734, 2095, 2865, 3645
DdeI CTNAG 7 cut(s) 171, 366, 462, 1909, 2546, 2924, 3864
DraI TTTAAA 2 cut(s) 3205, 3385
DrdI GACNNNNNNGTC 1 cut(s) 119
DseDI GACNNNNNNGTC 1 cut(s) 119
EaeI YGGCCR 1 cut(s) 722
Eam1104I CTCTTC 6 cut(s) 20, 83, 683, 1216, 2742, 3733
EarI CTCTTC 6 cut(s) 20, 83, 683, 1216, 2742, 3733
EciI GGCGGA 1 cut(s) 2405
Eco105I TACGTA 1 cut(s) 737
Eco130I CCWWGG 2 cut(s) 327, 1610
Eco147I AGGCCT 2 cut(s) 751, 3862
Eco24I GRGCYC 1 cut(s) 2557
Eco32I GATATC 1 cut(s) 3256
Eco47I GGWCC 2 cut(s) 1484, 3328
Eco57I CTGAAG 4 cut(s) 129, 1422, 2463, 2742
Eco88I CYCGRG 1 cut(s) 2292
EcoO109I RGGNCCY 1 cut(s) 3374
EcoRII CCWGG 1 cut(s) 547
EcoRV GATATC 1 cut(s) 3256
EcoT14I CCWWGG 2 cut(s) 327, 1610
EcoT22I ATGCAT 1 cut(s) 1007
EcoT38I GRGCYC 1 cut(s) 2557
ErhI CCWWGG 2 cut(s) 327, 1610
Esp3I CGTCTC 2 cut(s) 1198, 3397
FalI AAGNNNNNCTT 2 cut(s) 1560, 1592
FaqI GGGAC 1 cut(s) 1661
FauI CCCGC 3 cut(s) 182, 968, 3313
FauNDI CATATG 2 cut(s) 2932, 3793
FbaI TGATCA 1 cut(s) 3484
FblI GTMKAC 6 cut(s) 1152, 2400, 3313, 3354, 3691, 3747
FokI GGATG 6 cut(s) 437, 811, 1141, 1781, 2300, 3287
FriOI GRGCYC 1 cut(s) 2557
FspAI RTGCGCAY 1 cut(s) 1733
FspBI CTAG 7 cut(s) 186, 759, 1173, 1601, 2567, 3266, 3398
FspI TGCGCA 2 cut(s) 1256, 1733
GlaI GCGC 3 cut(s) 1256, 1733, 2627
GsaI CCCAGC 1 cut(s) 1502
GsuI CTGGAG 1 cut(s) 2541
HaeIII GGCC 9 cut(s) 392, 703, 724, 751, 2411, 2604, 2788, 3375, 3862
HapII CCGG 3 cut(s) 721, 2657, 2756
HgaI GACGC 1 cut(s) 1215
HhaI GCGC 3 cut(s) 1257, 1734, 2628
Hin1I GRCGYC 1 cut(s) 3406
Hin6I GCGC 3 cut(s) 1255, 1732, 2626
HinP1I GCGC 3 cut(s) 1255, 1732, 2626
HincII GTYRAC 2 cut(s) 441, 1244
HindII GTYRAC 2 cut(s) 441, 1244
HindIII AAGCTT 3 cut(s) 1330, 1574, 1616
HpaII CCGG 3 cut(s) 721, 2657, 2756
Hpy99I CGWCG 2 cut(s) 275, 1051
HpyAV CCTTC 5 cut(s) 741, 1554, 2275, 2662, 3812
HpyCH4III ACNGT 9 cut(s) 40, 119, 500, 982, 3141, 3332, 3560, 3583, 3644
HpyF10VI GCNNNNNNNGC 4 cut(s) 510, 541, 1573, 1624
HpyF3I CTNAG 7 cut(s) 171, 366, 462, 1909, 2546, 2924, 3864
Hsp92I GRCGYC 1 cut(s) 3406
HspAI GCGC 3 cut(s) 1255, 1732, 2626
Ksp22I TGATCA 1 cut(s) 3484
LguI GCTCTTC 1 cut(s) 1216
LmnI GCTCC 2 cut(s) 895, 2560
LweI GCATC 7 cut(s) 972, 1002, 2278, 2742, 3059, 3137, 3485
MabI ACCWGGT 1 cut(s) 547
MaeI CTAG 7 cut(s) 186, 759, 1173, 1601, 2567, 3266, 3398
MaeIII GTNAC 6 cut(s) 570, 1460, 1883, 3214, 3407, 3554
MbiI CCGCTC 1 cut(s) 3308
MfeI CAATTG 1 cut(s) 1622
MflI RGATCY 3 cut(s) 144, 412, 3509
MhlI GDGCHC 3 cut(s) 857, 2557, 3449
MlyI GAGTC 4 cut(s) 687, 769, 2069, 3044
MmeI TCCRAC 4 cut(s) 34, 1685, 1774, 2026
Mph1103I ATGCAT 1 cut(s) 1007
MroXI GAANNNNTTC 3 cut(s) 132, 1756, 2727
MslI CAYNNNNRTG 1 cut(s) 3414
MspA1I CMGCKG 1 cut(s) 1983
MspI CCGG 3 cut(s) 721, 2657, 2756
MspR9I CCNGG 2 cut(s) 549, 2657
MunI CAATTG 1 cut(s) 1622
MvaI CCWGG 1 cut(s) 549
MwoI GCNNNNNNNGC 4 cut(s) 510, 541, 1573, 1624
NciI CCSGG 1 cut(s) 2657
NcoI CCATGG 1 cut(s) 1610
NdeI CATATG 2 cut(s) 2932, 3793
NheI GCTAGC 1 cut(s) 1172
NlaIV GGNNCC 8 cut(s) 60, 768, 897, 958, 1095, 2556, 2703, 3153
NmeAIII GCCGAG 1 cut(s) 2770
NmuCI GTSAC 2 cut(s) 570, 3407
NsbI TGCGCA 2 cut(s) 1256, 1733
NsiI ATGCAT 1 cut(s) 1007
NspI RCATGY 3 cut(s) 665, 2191, 3546
NspV TTCGAA 2 cut(s) 18, 2811
PaeR7I CTCGAG 1 cut(s) 2292
PagI TCATGA 2 cut(s) 3415, 3679
PceI AGGCCT 2 cut(s) 751, 3862
PciI ACATGT 1 cut(s) 3542
PciSI GCTCTTC 1 cut(s) 1216
PdmI GAANNNNTTC 3 cut(s) 132, 1756, 2727
PflMI CCANNNNNTGG 1 cut(s) 2041
PleI GAGTC 4 cut(s) 687, 769, 2068, 3043
PpsI GAGTC 4 cut(s) 687, 769, 2068, 3043
Ppu21I YACGTR 4 cut(s) 737, 2182, 2740, 3358
PscI ACATGT 1 cut(s) 3542
Psp1406I AACGTT 2 cut(s) 181, 3705
Psp6I CCWGG 1 cut(s) 547
PspFI CCCAGC 1 cut(s) 1498
PspGI CCWGG 1 cut(s) 547
PspN4I GGNNCC 8 cut(s) 60, 768, 897, 958, 1095, 2556, 2703, 3153
PspPI GGNCC 4 cut(s) 390, 1484, 3328, 3374
PstI CTGCAG 1 cut(s) 1861
PsuI RGATCY 3 cut(s) 144, 412, 3509
PvuII CAGCTG 1 cut(s) 1983
RsaI GTAC 6 cut(s) 45, 614, 735, 2096, 2866, 3646
RsaNI GTAC 6 cut(s) 44, 613, 734, 2095, 2865, 3645
RseI CAYNNNNRTG 1 cut(s) 3414
SapI GCTCTTC 1 cut(s) 1216
Sau96I GGNCC 4 cut(s) 390, 1484, 3328, 3374
SchI GAGTC 4 cut(s) 687, 769, 2069, 3044
ScrFI CCNGG 2 cut(s) 549, 2657
SduI GDGCHC 3 cut(s) 857, 2557, 3449
SexAI ACCWGGT 1 cut(s) 547
SfaNI GCATC 7 cut(s) 972, 1002, 2278, 2742, 3059, 3137, 3485
SfcI CTRYAG 2 cut(s) 1078, 1857
Sfr274I CTCGAG 1 cut(s) 2292
SfuI TTCGAA 2 cut(s) 18, 2811
SinI GGWCC 2 cut(s) 1484, 3328
SlaI CTCGAG 1 cut(s) 2292
SmiMI CAYNNNNRTG 1 cut(s) 3414
SmlI CTYRAG 6 cut(s) 695, 1577, 2292, 2537, 2639, 3065
SmoI CTYRAG 6 cut(s) 695, 1577, 2292, 2537, 2639, 3065
SnaBI TACGTA 1 cut(s) 737
SpeI ACTAGT 1 cut(s) 3265
SseBI AGGCCT 2 cut(s) 751, 3862
SsiI CCGC 8 cut(s) 189, 393, 504, 961, 2390, 3149, 3306, 3674
SspI AATATT 3 cut(s) 619, 2579, 2770
SspMI CTAG 7 cut(s) 186, 759, 1173, 1601, 2567, 3266, 3398
StuI AGGCCT 2 cut(s) 751, 3862
StyD4I CCNGG 2 cut(s) 547, 2655
StyI CCWWGG 2 cut(s) 327, 1610
TaaI ACNGT 9 cut(s) 40, 119, 500, 982, 3141, 3332, 3560, 3583, 3644
TaqII GACCGA 1 cut(s) 3340
TatI WGTACW 2 cut(s) 43, 612
TauI GCSGC 1 cut(s) 395
TscAI CASTG 3 cut(s) 124, 295, 1519
TseFI GTSAC 2 cut(s) 570, 3407
Tsp45I GTSAC 2 cut(s) 570, 3407
TspGWI ACGGA 2 cut(s) 700, 1216
TspRI CASTG 3 cut(s) 124, 295, 1519
Van91I CCANNNNNTGG 1 cut(s) 2041
VneI GTGCAC 1 cut(s) 3445
VpaK11BI GGWCC 2 cut(s) 1484, 3328
XapI RAATTY 9 cut(s) 217, 371, 917, 1021, 1670, 1754, 2158, 2233, 2495
XceI RCATGY 3 cut(s) 665, 2191, 3546
XcmI CCANNNNNNNNNTGG 2 cut(s) 325, 2042
XhoI CTCGAG 1 cut(s) 2292
XmiI GTMKAC 6 cut(s) 1152, 2400, 3313, 3354, 3691, 3747
XmnI GAANNNNTTC 3 cut(s) 132, 1756, 2727
XspI CTAG 7 cut(s) 186, 759, 1173, 1601, 2567, 3266, 3398
ZraI GACGTC 1 cut(s) 3407
Zsp2I ATGCAT 1 cut(s) 1007
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.