FvH4_5g29920

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0007434
Physical Location & Seq
Forward (+)
20794992 .. 20799235
4244 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g29920.t1

Sequence Viewer

Length: 1065 bp
ATGGCAAAAGGTAGCAGGGGACGACGCAGGTTTGCTTCCCGGCAATACAGGTCAGCTCCATACCCATTGCGGTCTAATCAAGATATCTTTGAGGATCTGTGCCCTAAGAAATGCTCCAAAAATTTGGAGAAAAAAGACTGGGAGGATGCAACATGTTCAGTGTGCATGGAGTACCCTCACAATGCCGTTCTTCTCCTATGTTCCTCCCATGACAAAGGTTGTCGTCCTTACATGTGTGGAACTAGTTTTCGACATTCCAACTGTCTTGACCAGTACAAGAAAGCTTACACAAAAATAGTGTCATCTGATCATGGACAGTCATTGCATGGCTCTGCCACTAATCCAATTGTGGTACCAGATTCTGGATTGCCAGTTGATAAGTGTGAAGCTACAGAGCTTGCATGTCCCCTCTGTAGGGGCCAGGTGAAAGGCTGGACTGTTCTTGAACCAGCACGAGAATATCTAGATGCTAAACAGAGAAGCTGCATGCAAGAAAACTGCTCATTTGTTGGAAACTACAAGGAGCTTAGGAAGCATGTTAAGGCAGAGCACCCTTCAGCTCGGCCACGTGAAGTGGATCCTATAGTAGAACAGAAATGGAGAAGCCTTGAACGTGAGCGAGAACAGGATGATGTGATCAGCACAATACAGTCGTCCATGCCAGGGGCAATGTTTTTCGGAGACTATGTAATAGAAGGAAATAGTTATGGATTTGATTCGGACGAAGAGGATGGTGGGTTTGACGCAGGAGATGCTGCAGGAAGGAATGCAGCGTTCGGGCTGGGTTTGGATGTAGTTCTTCTATGGCATGCATTTGGATCCTCCAGACGGATGAGGCAGCATGAGAGGGCATTCCACCAGGCATCAGATGGTAGTGTTGGCATCCGGCACACGACTCCTATTGGTGGTTTGGATTCCTCTGATCAAGATGACGACAATGAGAGTAACGATGATGATGATGGCGGCATGTCATTGGTGAGCCGGCTCCGGCGTCGTGGCAGGGTCTTGTTGGGACGCTCTGGGAGGAGACGTAGACATAGAGAAGCAAGTAGTGAGGAACAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

355

Amino Acids

39.76

Weight (kDa)

6.64

Isoelectric Point (pI)

63.19

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 48 - 214 7.4e-72 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 18
Acc65I GGTACC 1 cut(s) 352
AccB1I GGYRCC 1 cut(s) 352
AccB7I CCANNNNNTGG 1 cut(s) 362
AccI GTMKAC 1 cut(s) 1033
AciI CCGC 2 cut(s) 70, 963
AclWI GGATC 5 cut(s) 102, 572, 585, 813, 826
AcoI YGGCCR 1 cut(s) 563
AcsI RAATTY 1 cut(s) 121
AcuI CTGAAG 1 cut(s) 540
AcvI CACGTG 1 cut(s) 569
AcyI GRCGYC 1 cut(s) 991
AfaI GTAC 3 cut(s) 173, 275, 354
AfiI CCNNNNNNNGG 6 cut(s) 362, 414, 415, 663, 828, 905
AflIII ACRYGT 2 cut(s) 152, 231
AgsI TTSAA 2 cut(s) 446, 611
AhlI ACTAGT 1 cut(s) 242
AjnI CCWGG 3 cut(s) 420, 661, 858
AluBI AGCT 7 cut(s) 56, 284, 389, 397, 483, 526, 560
AluI AGCT 7 cut(s) 56, 284, 389, 397, 483, 526, 560
Alw21I GWGCWC 1 cut(s) 552
Alw26I GTCTC 2 cut(s) 675, 1021
AlwI GGATC 5 cut(s) 102, 572, 585, 813, 826
AlwNI CAGNNNCTG 1 cut(s) 362
AoxI GGCC 2 cut(s) 418, 563
ApeKI GCWGC 4 cut(s) 483, 755, 770, 838
ApoI RAATTY 1 cut(s) 121
ArsI GACNNNNNNTTYG 2 cut(s) 284, 316
Asp718I GGTACC 1 cut(s) 352
AspS9I GGNCC 1 cut(s) 418
AsuC2I CCSGG 1 cut(s) 40
AsuHPI GGTGA 2 cut(s) 436, 988
BaeGI GKGCMC 1 cut(s) 104
BamHI GGATCC 2 cut(s) 577, 818
BanI GGYRCC 1 cut(s) 352
BauI CACGAG 1 cut(s) 453
BbrPI CACGTG 1 cut(s) 569
Bbv12I GWGCWC 1 cut(s) 552
BbvI GCAGC 4 cut(s) 470, 742, 782, 850
BccI CCATC 3 cut(s) 725, 863, 953
BceAI ACGGC 1 cut(s) 170
BciT130I CCWGG 3 cut(s) 422, 663, 860
BclI TGATCA 3 cut(s) 307, 636, 922
BcnI CCSGG 1 cut(s) 40
BcoDI GTCTC 2 cut(s) 675, 1021
BcuI ACTAGT 1 cut(s) 242
BfaI CTAG 2 cut(s) 243, 464
BfmI CTRYAG 4 cut(s) 390, 412, 582, 756
BfuAI ACCTGC 1 cut(s) 18
BisI GCNGC 5 cut(s) 484, 756, 771, 839, 964
BlsI GCNGC 5 cut(s) 485, 757, 772, 840, 965
Bme1390I CCNGG 4 cut(s) 40, 422, 663, 860
BmgT120I GGNCC 1 cut(s) 418
BmiI GGNNCC 5 cut(s) 354, 419, 579, 820, 986
BmrFI CCNGG 4 cut(s) 40, 422, 663, 860
BmrI ACTGGG 1 cut(s) 148
BmsI GCATC 5 cut(s) 136, 457, 742, 872, 891
BmuI ACTGGG 1 cut(s) 148
BpmI CTGGAG 1 cut(s) 808
Bpu10I CCTNAGC 1 cut(s) 527
BpuMI CCSGG 1 cut(s) 40
BsaAI YACGTR 1 cut(s) 569
BsaHI GRCGYC 1 cut(s) 991
BsaJI CCNNGG 1 cut(s) 662
BsaXI ACNNNNNCTCC 4 cut(s) 672, 702, 1015, 1045
Bsc4I CCNNNNNNNGG 6 cut(s) 362, 414, 415, 663, 828, 905
Bse118I RCCGGY 1 cut(s) 981
Bse1I ACTGG 3 cut(s) 143, 271, 371
Bse3DI GCAATG 3 cut(s) 65, 320, 675
BseBI CCWGG 3 cut(s) 422, 663, 860
BseDI CCNNGG 1 cut(s) 662
BseGI GGATG 6 cut(s) 151, 634, 736, 796, 837, 882
BseLI CCNNNNNNNGG 6 cut(s) 362, 414, 415, 663, 828, 905
BseMI GCAATG 3 cut(s) 65, 320, 675
BseNI ACTGG 3 cut(s) 143, 271, 371
BseRI GAGGAG 1 cut(s) 1039
BseSI GKGCMC 1 cut(s) 104
BseXI GCAGC 4 cut(s) 470, 742, 782, 850
BseYI CCCAGC 1 cut(s) 781
BshFI GGCC 2 cut(s) 420, 565
BshNI GGYRCC 1 cut(s) 352
BsiHKAI GWGCWC 1 cut(s) 552
BsiSI CCGG 4 cut(s) 40, 886, 982, 988
BslFI GGGAC 3 cut(s) 33, 390, 1026
BslI CCNNNNNNNGG 6 cut(s) 362, 414, 415, 663, 828, 905
BsmAI GTCTC 2 cut(s) 675, 1021
BsmBI CGTCTC 1 cut(s) 1021
BsmFI GGGAC 3 cut(s) 33, 390, 1026
BsmI GAATGC 2 cut(s) 772, 851
BsnI GGCC 2 cut(s) 420, 565
Bsp1286I GDGCHC 2 cut(s) 104, 552
Bsp143I GATC 6 cut(s) 94, 307, 577, 636, 818, 922
BspACI CCGC 2 cut(s) 70, 963
BspANI GGCC 2 cut(s) 420, 565
BspLI GGNNCC 5 cut(s) 354, 419, 579, 820, 986
BspMAI CTGCAG 1 cut(s) 760
BspMI ACCTGC 1 cut(s) 18
BspPI GGATC 5 cut(s) 102, 572, 585, 813, 826
BspT107I GGYRCC 1 cut(s) 352
BsrDI GCAATG 3 cut(s) 65, 320, 675
BsrFI RCCGGY 1 cut(s) 981
BsrI ACTGG 3 cut(s) 143, 271, 371
BssAI RCCGGY 1 cut(s) 981
BssECI CCNNGG 1 cut(s) 662
BssMI GATC 6 cut(s) 94, 307, 577, 636, 818, 922
BssNI GRCGYC 1 cut(s) 991
BssSI CACGAG 1 cut(s) 453
Bst2BI CACGAG 1 cut(s) 453
Bst2UI CCWGG 3 cut(s) 422, 663, 860
Bst4CI ACNGT 4 cut(s) 263, 318, 439, 651
Bst6I CTCTTC 1 cut(s) 720
BstACI GRCGYC 1 cut(s) 991
BstAPI GCANNNNNTGC 1 cut(s) 752
BstBAI YACGTR 1 cut(s) 569
BstC8I GCNNGC 4 cut(s) 399, 488, 810, 983
BstDEI CTNAG 2 cut(s) 105, 527
BstF5I GGATG 6 cut(s) 151, 634, 736, 796, 837, 882
BstKTI GATC 6 cut(s) 97, 310, 580, 639, 821, 925
BstMAI GTCTC 2 cut(s) 675, 1021
BstMBI GATC 6 cut(s) 94, 307, 577, 636, 818, 922
BstMWI GCNNNNNNNGC 2 cut(s) 532, 752
BstNI CCWGG 3 cut(s) 422, 663, 860
BstNSI RCATGY 7 cut(s) 156, 235, 405, 490, 539, 812, 970
BstSCI CCNGG 4 cut(s) 38, 420, 661, 858
BstSFI CTRYAG 4 cut(s) 390, 412, 582, 756
BstSLI GKGCMC 1 cut(s) 104
BstV1I GCAGC 4 cut(s) 470, 742, 782, 850
BstX2I RGATCY 3 cut(s) 94, 577, 818
BstXI CCANNNNNNTGG 1 cut(s) 124
BstYI RGATCY 3 cut(s) 94, 577, 818
BsuRI GGCC 2 cut(s) 420, 565
BtsCI GGATG 6 cut(s) 151, 634, 736, 796, 837, 882
BtsIMutI CAGTG 1 cut(s) 165
BveI ACCTGC 1 cut(s) 18
Cac8I GCNNGC 4 cut(s) 399, 488, 810, 983
CaiI CAGNNNCTG 1 cut(s) 362
Cfr10I RCCGGY 1 cut(s) 981
Cfr13I GGNCC 1 cut(s) 418
CseI GACGC 4 cut(s) 33, 752, 980, 1023
Csp6I GTAC 3 cut(s) 172, 274, 353
CviQI GTAC 3 cut(s) 172, 274, 353
DdeI CTNAG 2 cut(s) 105, 527
DpnI GATC 6 cut(s) 96, 309, 579, 638, 820, 924
DpnII GATC 6 cut(s) 94, 307, 577, 636, 818, 922
EaeI YGGCCR 1 cut(s) 563
Eam1104I CTCTTC 1 cut(s) 720
EarI CTCTTC 1 cut(s) 720
Eco32I GATATC 1 cut(s) 85
Eco57I CTGAAG 1 cut(s) 540
Eco72I CACGTG 1 cut(s) 569
EcoRII CCWGG 3 cut(s) 420, 661, 858
EcoRV GATATC 1 cut(s) 85
EcoT22I ATGCAT 1 cut(s) 814
Esp3I CGTCTC 1 cut(s) 1021
FaqI GGGAC 3 cut(s) 33, 390, 1026
FbaI TGATCA 3 cut(s) 307, 636, 922
FblI GTMKAC 1 cut(s) 1033
Fnu4HI GCNGC 5 cut(s) 484, 756, 771, 839, 964
FokI GGATG 6 cut(s) 158, 641, 743, 803, 844, 869
Fsp4HI GCNGC 5 cut(s) 484, 756, 771, 839, 964
FspBI CTAG 2 cut(s) 243, 464
GluI GCNGC 5 cut(s) 484, 756, 771, 839, 964
GsaI CCCAGC 1 cut(s) 785
GsuI CTGGAG 1 cut(s) 808
HaeIII GGCC 2 cut(s) 420, 565
HapII CCGG 4 cut(s) 40, 886, 982, 988
HgaI GACGC 4 cut(s) 33, 752, 980, 1023
Hin1I GRCGYC 1 cut(s) 991
HindIII AAGCTT 1 cut(s) 282
HinfI GANTC 4 cut(s) 359, 716, 895, 914
HpaII CCGG 4 cut(s) 40, 886, 982, 988
HphI GGTGA 2 cut(s) 436, 988
Hpy166II GTNNAC 1 cut(s) 1034
Hpy188I TCNGA 5 cut(s) 307, 680, 721, 868, 922
Hpy188III TCNNGA 7 cut(s) 80, 266, 363, 443, 464, 825, 926
Hpy8I GTNNAC 1 cut(s) 1034
Hpy99I CGWCG 2 cut(s) 27, 996
HpyAV CCTTC 3 cut(s) 564, 689, 756
HpyCH4III ACNGT 4 cut(s) 263, 318, 439, 651
HpyCH4IV ACGT 3 cut(s) 568, 613, 1030
HpyCH4V TGCA 9 cut(s) 149, 165, 325, 401, 486, 490, 758, 770, 812
HpyF10VI GCNNNNNNNGC 2 cut(s) 532, 752
HpyF3I CTNAG 2 cut(s) 105, 527
HpySE526I ACGT 3 cut(s) 568, 613, 1030
Hsp92I GRCGYC 1 cut(s) 991
KpnI GGTACC 1 cut(s) 356
KroI GCCGGC 1 cut(s) 981
KroNI GCCGGC 1 cut(s) 983
Ksp22I TGATCA 3 cut(s) 307, 636, 922
Kzo9I GATC 6 cut(s) 94, 307, 577, 636, 818, 922
LmnI GCTCC 4 cut(s) 61, 119, 523, 990
Lsp1109I GCAGC 4 cut(s) 470, 742, 782, 850
LweI GCATC 5 cut(s) 136, 457, 742, 872, 891
MaeI CTAG 2 cut(s) 243, 464
MaeII ACGT 3 cut(s) 568, 613, 1030
MaeIII GTNAC 1 cut(s) 944
MalI GATC 6 cut(s) 96, 309, 579, 638, 820, 924
MboI GATC 6 cut(s) 94, 307, 577, 636, 818, 922
MboII GAAGA 3 cut(s) 182, 737, 791
MfeI CAATTG 1 cut(s) 345
MflI RGATCY 3 cut(s) 94, 577, 818
MhlI GDGCHC 2 cut(s) 104, 552
MluCI AATT 2 cut(s) 121, 345
MlyI GAGTC 1 cut(s) 889
MmeI TCCRAC 2 cut(s) 282, 490
Mph1103I ATGCAT 1 cut(s) 814
MroNI GCCGGC 1 cut(s) 981
MseI TTAA 1 cut(s) 540
MspI CCGG 4 cut(s) 40, 886, 982, 988
MspR9I CCNGG 4 cut(s) 40, 422, 663, 860
MunI CAATTG 1 cut(s) 345
Mva1269I GAATGC 2 cut(s) 772, 851
MvaI CCWGG 3 cut(s) 422, 663, 860
MwoI GCNNNNNNNGC 2 cut(s) 532, 752
NaeI GCCGGC 1 cut(s) 983
NciI CCSGG 1 cut(s) 40
NdeII GATC 6 cut(s) 94, 307, 577, 636, 818, 922
NgoMIV GCCGGC 1 cut(s) 981
NlaIV GGNNCC 5 cut(s) 354, 419, 579, 820, 986
NmeAIII GCCGAG 1 cut(s) 541
NsiI ATGCAT 1 cut(s) 814
NspI RCATGY 7 cut(s) 156, 235, 405, 490, 539, 812, 970
PaeI GCATGC 2 cut(s) 490, 812
PciI ACATGT 2 cut(s) 152, 231
PctI GAATGC 2 cut(s) 772, 851
PdiI GCCGGC 1 cut(s) 983
PfeI GAWTC 3 cut(s) 359, 716, 914
PflMI CCANNNNNTGG 1 cut(s) 362
PkrI GCNGC 5 cut(s) 485, 757, 772, 840, 965
PleI GAGTC 1 cut(s) 889
PmaCI CACGTG 1 cut(s) 569
PmlI CACGTG 1 cut(s) 569
PpsI GAGTC 1 cut(s) 889
Ppu21I YACGTR 1 cut(s) 569
PscI ACATGT 2 cut(s) 152, 231
Psp6I CCWGG 3 cut(s) 420, 661, 858
PspCI CACGTG 1 cut(s) 569
PspFI CCCAGC 1 cut(s) 781
PspGI CCWGG 3 cut(s) 420, 661, 858
PspN4I GGNNCC 5 cut(s) 354, 419, 579, 820, 986
PspPI GGNCC 1 cut(s) 418
PstI CTGCAG 1 cut(s) 760
PstNI CAGNNNCTG 1 cut(s) 362
PsuI RGATCY 3 cut(s) 94, 577, 818
RsaI GTAC 3 cut(s) 173, 275, 354
RsaNI GTAC 3 cut(s) 172, 274, 353
SaqAI TTAA 1 cut(s) 540
SatI GCNGC 5 cut(s) 484, 756, 771, 839, 964
Sau3AI GATC 6 cut(s) 94, 307, 577, 636, 818, 922
Sau96I GGNCC 1 cut(s) 418
SchI GAGTC 1 cut(s) 889
ScrFI CCNGG 4 cut(s) 40, 422, 663, 860
SduI GDGCHC 2 cut(s) 104, 552
SfaNI GCATC 5 cut(s) 136, 457, 742, 872, 891
SfcI CTRYAG 4 cut(s) 390, 412, 582, 756
SpeI ACTAGT 1 cut(s) 242
SphI GCATGC 2 cut(s) 490, 812
Sse9I AATT 2 cut(s) 121, 345
SsiI CCGC 2 cut(s) 70, 963
SspMI CTAG 2 cut(s) 243, 464
StyD4I CCNGG 4 cut(s) 38, 420, 661, 858
TaaI ACNGT 4 cut(s) 263, 318, 439, 651
TaiI ACGT 3 cut(s) 571, 616, 1033
TaqI TCGA 1 cut(s) 250
TasI AATT 2 cut(s) 121, 345
TatI WGTACW 1 cut(s) 273
TauI GCSGC 1 cut(s) 966
TfiI GAWTC 3 cut(s) 359, 716, 914
Tru1I TTAA 1 cut(s) 540
Tru9I TTAA 1 cut(s) 540
TscAI CASTG 1 cut(s) 165
TseI GCWGC 4 cut(s) 483, 755, 770, 838
TspGWI ACGGA 1 cut(s) 844
TspRI CASTG 1 cut(s) 165
Van91I CCANNNNNTGG 1 cut(s) 362
XapI RAATTY 1 cut(s) 121
XbaI TCTAGA 1 cut(s) 463
XceI RCATGY 7 cut(s) 156, 235, 405, 490, 539, 812, 970
XcmI CCANNNNNNNNNTGG 1 cut(s) 866
XmiI GTMKAC 1 cut(s) 1033
XspI CTAG 2 cut(s) 243, 464
Zsp2I ATGCAT 1 cut(s) 814
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.