pycom08g19780

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr8
OG0007434
Physical Location & Seq
Reverse (-)
19735892 .. 19736824
933 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom08g19780.1

Sequence Viewer

Length: 933 bp
ATGGAGTATCCTCACAATGCTGTTCTTCTCCTTTGTTCTTCTCATGACAAAGGTTGCCGTCCCTACATGTGTGGAACTAGTTTCCGACATTCCAATTGTCTTGGCCAGTACAAGAAAGCTTATACTAAAATTGTGTCATCTGATCTTGGACAACCATTGCTTGGCTCCAACAACGATCCAATTATGGTACCGGATGCTGGATGGCCAGTACAAAAGTGTGAAGTCACAGAACTTGCATGCCCCCTCTGTAGGGGCGAGGTGAAGGGCTGGACTGTTCTTGAACCAGCACGAGATTATCTAAATACAAAAAGGAGAAGCTGCATGCAAGAAAATTGTTCATTTGTCGGAAATTACAAGGAGCTTAAAAGGCATGTGAAGGAAGAGCACCCCTCTGCGCGCCCACGAGAAGTGGATCCAGACCTCGAAGAGAAATGGAGAAGTCTTGAACATGAGCGTGAGACGAACGATCTGATGAGCACAATCCAGTCTTCCATGCCAGGTGCAATGGTTTTTGGAGATTATGTAATTGAAAGAAATAATTATGGTTTGGATATGGACGAAGAGGATGGTGGCTTTAATGCAGATGCTCCAGAAAGAAATGGGGGGTTCGGGCTGGGATTCGATGGCAATCTGGTGAACTTCTTTTTCCTATTACATGCATTTGGGCCATCAAGGACTGACCTGACAAGACGTCTAAGACCGCCTGAGAGGGCCTTCCACCACTCATTGGACAACAGTGCTGCTGGCATCCGGCACACAGCTCCTGTTGGCGGTTTGGATTCCTCTGATCAAGATGAAGGGAATGATAGTGATGGTGATGATGACGGCGGTGGTATGTCTTTCGCGAGCCGCCTTCGGCGTCATGGCAGGGTGTTGTTGGGACGTTCTGGGAGGAGACGTATGCGTAGAGAAGGAAACAGTGATGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

311

Amino Acids

34.8

Weight (kDa)

5.91

Isoelectric Point (pI)

58.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 1 - 159 4.1e-65 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 694
Acc65I GGTACC 1 cut(s) 187
AccB1I GGYRCC 1 cut(s) 187
AccB7I CCANNNNNTGG 2 cut(s) 161, 727
AccII CGCG 2 cut(s) 397, 845
AciI CCGC 4 cut(s) 701, 771, 828, 850
AclWI GGATC 3 cut(s) 170, 407, 420
AcoI YGGCCR 2 cut(s) 103, 203
AcyI GRCGYC 2 cut(s) 691, 859
AfaI GTAC 3 cut(s) 110, 189, 210
AfiI CCNNNNNNNGG 6 cut(s) 161, 197, 249, 250, 727, 770
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 3 cut(s) 281, 446, 530
AhdI GACNNNNNGTC 1 cut(s) 690
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 1 cut(s) 496
AluBI AGCT 4 cut(s) 119, 318, 361, 761
AluI AGCT 4 cut(s) 119, 318, 361, 761
Alw21I GWGCWC 2 cut(s) 387, 479
Alw26I GTCTC 2 cut(s) 452, 889
AlwI GGATC 3 cut(s) 170, 407, 420
AlwNI CAGNNNCTG 1 cut(s) 764
AoxI GGCC 4 cut(s) 103, 203, 665, 711
ApeKI GCWGC 2 cut(s) 318, 740
Asp718I GGTACC 1 cut(s) 187
AspLEI GCGC 2 cut(s) 397, 399
AspS9I GGNCC 2 cut(s) 665, 711
AsuHPI GGTGA 3 cut(s) 271, 646, 827
BalI TGGCCA 2 cut(s) 105, 205
BamHI GGATCC 1 cut(s) 412
BanI GGYRCC 1 cut(s) 187
BauI CACGAG 2 cut(s) 288, 402
BbsI GAAGAC 1 cut(s) 480
Bbv12I GWGCWC 2 cut(s) 387, 479
BbvI GCAGC 2 cut(s) 305, 727
BccI CCATC 5 cut(s) 195, 560, 617, 676, 806
BceAI ACGGC 2 cut(s) 42, 841
BciT130I CCWGG 1 cut(s) 498
BciVI GTATCC 1 cut(s) 18
BclI TGATCA 1 cut(s) 787
BcoDI GTCTC 2 cut(s) 452, 889
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 247
BfuI GTATCC 1 cut(s) 18
BisI GCNGC 3 cut(s) 319, 741, 850
BlsI GCNGC 3 cut(s) 320, 742, 851
Bme1390I CCNGG 1 cut(s) 498
BmeRI GACNNNNNGTC 1 cut(s) 690
BmgT120I GGNCC 2 cut(s) 665, 711
BmiI GGNNCC 3 cut(s) 166, 189, 414
BmrFI CCNGG 1 cut(s) 498
BmsI GCATC 3 cut(s) 184, 574, 756
BpiI GAAGAC 1 cut(s) 480
BplI GAGNNNNNCTC 2 cut(s) 374, 406
BpmI CTGGAG 1 cut(s) 573
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 2 cut(s) 691, 859
BsaWI WCCGGW 1 cut(s) 190
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 883, 913
Bsc4I CCNNNNNNNGG 6 cut(s) 161, 197, 249, 250, 727, 770
Bse1I ACTGG 3 cut(s) 106, 206, 484
Bse3DI GCAATG 2 cut(s) 155, 510
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 1 cut(s) 498
BseGI GGATG 4 cut(s) 199, 206, 571, 747
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 6 cut(s) 161, 197, 249, 250, 727, 770
BseMI GCAATG 2 cut(s) 155, 510
BseMII CTCAG 1 cut(s) 696
BseNI ACTGG 3 cut(s) 106, 206, 484
BsePI GCGCGC 1 cut(s) 395
BseRI GAGGAG 1 cut(s) 907
BseXI GCAGC 2 cut(s) 305, 727
BseYI CCCAGC 1 cut(s) 613
Bsh1236I CGCG 2 cut(s) 397, 845
BshFI GGCC 4 cut(s) 105, 205, 667, 713
BshNI GGYRCC 1 cut(s) 187
BsiHKAI GWGCWC 2 cut(s) 387, 479
BsiSI CCGG 2 cut(s) 191, 751
BslFI GGGAC 2 cut(s) 45, 894
BslI CCNNNNNNNGG 6 cut(s) 161, 197, 249, 250, 727, 770
BsmAI GTCTC 2 cut(s) 452, 889
BsmBI CGTCTC 2 cut(s) 452, 889
BsmFI GGGAC 2 cut(s) 45, 894
BsnI GGCC 4 cut(s) 105, 205, 667, 713
Bsp1286I GDGCHC 2 cut(s) 387, 479
Bsp143I GATC 5 cut(s) 142, 175, 412, 466, 787
Bsp68I TCGCGA 1 cut(s) 845
BspACI CCGC 4 cut(s) 701, 771, 828, 850
BspANI GGCC 4 cut(s) 105, 205, 667, 713
BspCNI CTCAG 1 cut(s) 697
BspFNI CGCG 2 cut(s) 397, 845
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 166, 189, 414
BspPI GGATC 3 cut(s) 170, 407, 420
BspQI GCTCTTC 1 cut(s) 375
BspT107I GGYRCC 1 cut(s) 187
BsrDI GCAATG 2 cut(s) 155, 510
BsrI ACTGG 3 cut(s) 106, 206, 484
BssHII GCGCGC 1 cut(s) 395
BssMI GATC 5 cut(s) 142, 175, 412, 466, 787
BssNI GRCGYC 2 cut(s) 691, 859
BssSI CACGAG 2 cut(s) 288, 402
Bst2BI CACGAG 2 cut(s) 288, 402
Bst2UI CCWGG 1 cut(s) 498
Bst4CI ACNGT 3 cut(s) 274, 737, 920
Bst6I CTCTTC 3 cut(s) 375, 420, 555
BstACI GRCGYC 2 cut(s) 691, 859
BstC8I GCNNGC 5 cut(s) 238, 323, 397, 745, 847
BstDEI CTNAG 2 cut(s) 695, 705
BstF5I GGATG 4 cut(s) 199, 206, 571, 747
BstFNI CGCG 2 cut(s) 397, 845
BstHHI GCGC 2 cut(s) 397, 399
BstKTI GATC 5 cut(s) 145, 178, 415, 469, 790
BstMAI GTCTC 2 cut(s) 452, 889
BstMBI GATC 5 cut(s) 142, 175, 412, 466, 787
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 1 cut(s) 498
BstNSI RCATGY 5 cut(s) 70, 240, 325, 374, 659
BstSCI CCNGG 1 cut(s) 496
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 2 cut(s) 397, 845
BstV1I GCAGC 2 cut(s) 305, 727
BstV2I GAAGAC 1 cut(s) 480
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuI GTATCC 1 cut(s) 18
BsuRI GGCC 4 cut(s) 105, 205, 667, 713
BtsCI GGATG 4 cut(s) 199, 206, 571, 747
BtsIMutI CAGTG 2 cut(s) 742, 925
BtuMI TCGCGA 1 cut(s) 845
Cac8I GCNNGC 5 cut(s) 238, 323, 397, 745, 847
CaiI CAGNNNCTG 1 cut(s) 764
CciI TCATGA 1 cut(s) 43
CfoI GCGC 2 cut(s) 397, 399
Cfr13I GGNCC 2 cut(s) 665, 711
CseI GACGC 1 cut(s) 848
Csp6I GTAC 3 cut(s) 109, 188, 209
CviAII CATG 9 cut(s) 44, 67, 237, 322, 371, 449, 493, 656, 863
CviQI GTAC 3 cut(s) 109, 188, 209
DdeI CTNAG 2 cut(s) 695, 705
DpnI GATC 5 cut(s) 144, 177, 414, 468, 789
DpnII GATC 5 cut(s) 142, 175, 412, 466, 787
DriI GACNNNNNGTC 1 cut(s) 690
EaeI YGGCCR 2 cut(s) 103, 203
Eam1104I CTCTTC 3 cut(s) 375, 420, 555
Eam1105I GACNNNNNGTC 1 cut(s) 690
EarI CTCTTC 3 cut(s) 375, 420, 555
EcoO109I RGGNCCY 1 cut(s) 711
EcoRII CCWGG 1 cut(s) 496
EcoT22I ATGCAT 1 cut(s) 661
Esp3I CGTCTC 2 cut(s) 452, 889
FaeI CATG 9 cut(s) 47, 70, 240, 325, 374, 452, 496, 659, 866
FaqI GGGAC 2 cut(s) 45, 894
FatI CATG 9 cut(s) 43, 66, 236, 321, 370, 448, 492, 655, 862
FbaI TGATCA 1 cut(s) 787
Fnu4HI GCNGC 3 cut(s) 319, 741, 850
FokI GGATG 4 cut(s) 206, 213, 578, 734
Fsp4HI GCNGC 3 cut(s) 319, 741, 850
FspBI CTAG 1 cut(s) 78
GlaI GCGC 2 cut(s) 396, 398
GluI GCNGC 3 cut(s) 319, 741, 850
GsaI CCCAGC 1 cut(s) 617
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 4 cut(s) 105, 205, 667, 713
HapII CCGG 2 cut(s) 191, 751
HgaI GACGC 1 cut(s) 848
HhaI GCGC 2 cut(s) 397, 399
Hin1I GRCGYC 2 cut(s) 691, 859
Hin1II CATG 9 cut(s) 47, 70, 240, 325, 374, 452, 496, 659, 866
Hin6I GCGC 2 cut(s) 395, 397
HinP1I GCGC 2 cut(s) 395, 397
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 2 cut(s) 618, 779
HpaII CCGG 2 cut(s) 191, 751
HphI GGTGA 3 cut(s) 271, 646, 827
Hpy166II GTNNAC 1 cut(s) 637
Hpy188I TCNGA 5 cut(s) 86, 142, 347, 471, 787
Hpy188III TCNNGA 7 cut(s) 44, 278, 416, 443, 590, 791, 844
Hpy8I GTNNAC 1 cut(s) 637
HpyAV CCTTC 6 cut(s) 256, 370, 724, 791, 863, 905
HpyCH4III ACNGT 3 cut(s) 274, 737, 920
HpyCH4IV ACGT 3 cut(s) 691, 883, 898
HpyCH4V TGCA 6 cut(s) 236, 321, 325, 503, 581, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 2 cut(s) 695, 705
HpySE526I ACGT 3 cut(s) 691, 883, 898
Hsp92I GRCGYC 2 cut(s) 691, 859
Hsp92II CATG 9 cut(s) 47, 70, 240, 325, 374, 452, 496, 659, 866
HspAI GCGC 2 cut(s) 395, 397
KpnI GGTACC 1 cut(s) 191
Ksp22I TGATCA 1 cut(s) 787
Kzo9I GATC 5 cut(s) 142, 175, 412, 466, 787
LguI GCTCTTC 1 cut(s) 375
LmnI GCTCC 4 cut(s) 170, 358, 592, 766
Lsp1109I GCAGC 2 cut(s) 305, 727
LweI GCATC 3 cut(s) 184, 574, 756
MaeI CTAG 1 cut(s) 78
MaeII ACGT 3 cut(s) 691, 883, 898
MaeIII GTNAC 1 cut(s) 223
MalI GATC 5 cut(s) 144, 177, 414, 468, 789
MboI GATC 5 cut(s) 142, 175, 412, 466, 787
MboII GAAGA 6 cut(s) 17, 30, 392, 437, 480, 572
MfeI CAATTG 1 cut(s) 94
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 2 cut(s) 387, 479
MlsI TGGCCA 2 cut(s) 105, 205
MluCI AATT 7 cut(s) 94, 129, 180, 331, 349, 525, 538
MluNI TGGCCA 2 cut(s) 105, 205
MmeI TCCRAC 3 cut(s) 109, 192, 325
MnlI CCTC 9 cut(s) 21, 250, 254, 400, 431, 556, 702, 793, 885
Mox20I TGGCCA 2 cut(s) 105, 205
Mph1103I ATGCAT 1 cut(s) 661
MscI TGGCCA 2 cut(s) 105, 205
MseI TTAA 2 cut(s) 363, 576
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 2 cut(s) 105, 205
MspI CCGG 2 cut(s) 191, 751
MspR9I CCNGG 1 cut(s) 498
MunI CAATTG 1 cut(s) 94
MvaI CCWGG 1 cut(s) 498
MvnI CGCG 2 cut(s) 397, 845
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 5 cut(s) 142, 175, 412, 466, 787
NlaIII CATG 9 cut(s) 47, 70, 240, 325, 374, 452, 496, 659, 866
NlaIV GGNNCC 3 cut(s) 166, 189, 414
NmuCI GTSAC 1 cut(s) 223
NruI TCGCGA 1 cut(s) 845
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 5 cut(s) 70, 240, 325, 374, 659
PaeI GCATGC 2 cut(s) 240, 325
PagI TCATGA 1 cut(s) 43
PauI GCGCGC 1 cut(s) 395
PciI ACATGT 1 cut(s) 66
PciSI GCTCTTC 1 cut(s) 375
PfeI GAWTC 2 cut(s) 618, 779
PflMI CCANNNNNTGG 2 cut(s) 161, 727
PkrI GCNGC 3 cut(s) 320, 742, 851
PscI ACATGT 1 cut(s) 66
Psp6I CCWGG 1 cut(s) 496
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 1 cut(s) 496
PspN4I GGNNCC 3 cut(s) 166, 189, 414
PspPI GGNCC 2 cut(s) 665, 711
PstNI CAGNNNCTG 1 cut(s) 764
PsuI RGATCY 1 cut(s) 412
PteI GCGCGC 1 cut(s) 395
RruI TCGCGA 1 cut(s) 845
RsaI GTAC 3 cut(s) 110, 189, 210
RsaNI GTAC 3 cut(s) 109, 188, 209
RseI CAYNNNNRTG 1 cut(s) 453
SapI GCTCTTC 1 cut(s) 375
SaqAI TTAA 2 cut(s) 363, 576
SatI GCNGC 3 cut(s) 319, 741, 850
Sau3AI GATC 5 cut(s) 142, 175, 412, 466, 787
Sau96I GGNCC 2 cut(s) 665, 711
ScrFI CCNGG 1 cut(s) 498
SduI GDGCHC 2 cut(s) 387, 479
SfaNI GCATC 3 cut(s) 184, 574, 756
SfcI CTRYAG 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 453
SpeI ACTAGT 1 cut(s) 77
SphI GCATGC 2 cut(s) 240, 325
Sse9I AATT 7 cut(s) 94, 129, 180, 331, 349, 525, 538
SsiI CCGC 4 cut(s) 701, 771, 828, 850
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 1 cut(s) 496
TaaI ACNGT 3 cut(s) 274, 737, 920
TaiI ACGT 3 cut(s) 694, 886, 901
TaqI TCGA 2 cut(s) 423, 621
TasI AATT 7 cut(s) 94, 129, 180, 331, 349, 525, 538
TatI WGTACW 2 cut(s) 108, 208
TauI GCSGC 1 cut(s) 852
TfiI GAWTC 2 cut(s) 618, 779
Tru1I TTAA 2 cut(s) 363, 576
Tru9I TTAA 2 cut(s) 363, 576
TscAI CASTG 2 cut(s) 742, 925
TseFI GTSAC 1 cut(s) 223
TseI GCWGC 2 cut(s) 318, 740
Tsp45I GTSAC 1 cut(s) 223
TspDTI ATGAA 2 cut(s) 327, 810
TspRI CASTG 2 cut(s) 742, 925
Van91I CCANNNNNTGG 2 cut(s) 161, 727
XceI RCATGY 5 cut(s) 70, 240, 325, 374, 659
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 692
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.