MD15G1419100.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0007434
Physical Location & Seq
Reverse (-)
52041360 .. 52045093
3734 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1419100.v1.1.491

Sequence Viewer

Length: 933 bp
ATGGAGTATCCTCACAATGCTGTCCTTCTCCTTTGTTCTTCTCATGACAAAGGTTGCCGTCCCTACATGTGTGGAACTAGTTTCCGGCATTCCAACTGTCTTGGCCAGTACAAGAAAGCTTATACTAAAATGGGGTCATCTGATCATGGACAACCATTGCTTGGCTCCAACAACAATCCAACTGCTGTACCAGATGCTGGATGGCCAGTACAGAGGTGTGAAGTTACAGAGCTTGCATGCCCCCTCTGTAGGGGCCAGGTGAAGGGCTGGACTGTTCTTGAACCAGCACGAGATTATCTAAATACAAAAAGGAGAAGCTGCATGCAAGAAAATTGCGCATTTGTCGGAAATTACAAGGAGCTTAGGAGGCATGTGAAGGAAGAACACCCCTCCGCGCGGCCACGAGAAGTGGATCCAGACCTCGAACAGAAATGGAGAAGTCTTGAACATGAGCGTGAGACGAACGATCTGATGAGCACAATCCAGTCTACCATGCCAGGGGCAATGGTTTTTGGAGATTATGTAATAGAAAGAAATAATTATGGTTTGGATACGGACGAAGAGGATGGTGGCTTTGATGCAGATGCTCCAGAAAGAAATGGGGGGTTCGGGCTGGGATTCGATGGCAATCTGGTGAACGTCTTTTTCCTATTACATGCATTTGGGCCATCAGGCACTGACCTGACAAGACGTCTACGGCAGCCAGAGAGGGCCTTCCACCACTCAGCGGACGGCAGTGCTGCTGGCATCAGGCACACAACTCCTATTGGTGGTTTAGATTCCTCTGATCAAGATGAAGAGAATGAAAGTGATGGTGATGACGACGGTGGTGGTATGTCGTTGGTGAGCCGCCTTCGGCGTCATGGGAGGGTGTTGTTGGGACGTTCTGGGAGGAGAGGTGCGCGTAGAGAAGGAAATAGTGATGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

311

Amino Acids

34.5

Weight (kDa)

5.86

Isoelectric Point (pI)

57.82

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 1 - 159 8.6e-66 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 694
Acc16I TGCGCA 1 cut(s) 337
AccB7I CCANNNNNTGG 2 cut(s) 161, 197
AccI GTMKAC 2 cut(s) 488, 694
AccII CGCG 3 cut(s) 395, 397, 904
AciI CCGC 4 cut(s) 393, 397, 728, 850
AclWI GGATC 2 cut(s) 407, 420
AcoI YGGCCR 3 cut(s) 103, 203, 398
AcyI GRCGYC 2 cut(s) 691, 859
AfaI GTAC 3 cut(s) 110, 189, 210
AfiI CCNNNNNNNGG 9 cut(s) 161, 197, 249, 250, 262, 396, 498, 727, 770
AflIII ACRYGT 1 cut(s) 66
AgsI TTSAA 2 cut(s) 281, 446
AhdI GACNNNNNGTC 1 cut(s) 690
AhlI ACTAGT 1 cut(s) 77
AjnI CCWGG 2 cut(s) 255, 496
AluBI AGCT 4 cut(s) 119, 232, 318, 361
AluI AGCT 4 cut(s) 119, 232, 318, 361
Alw21I GWGCWC 1 cut(s) 479
Alw26I GTCTC 1 cut(s) 452
AlwI GGATC 2 cut(s) 407, 420
AlwNI CAGNNNCTG 2 cut(s) 197, 677
AoxI GGCC 6 cut(s) 103, 203, 253, 398, 665, 711
ApeKI GCWGC 3 cut(s) 318, 700, 740
AspLEI GCGC 3 cut(s) 338, 397, 904
AspS9I GGNCC 3 cut(s) 253, 665, 711
AsuHPI GGTGA 4 cut(s) 271, 646, 827, 856
BaeI ACNNNNGTAYC 2 cut(s) 171, 204
BalI TGGCCA 2 cut(s) 105, 205
BamHI GGATCC 1 cut(s) 412
BauI CACGAG 2 cut(s) 288, 402
Bbv12I GWGCWC 1 cut(s) 479
BbvI GCAGC 3 cut(s) 305, 712, 727
BccI CCATC 5 cut(s) 195, 560, 617, 676, 806
BceAI ACGGC 3 cut(s) 42, 713, 748
BciT130I CCWGG 2 cut(s) 257, 498
BciVI GTATCC 2 cut(s) 18, 544
BclI TGATCA 2 cut(s) 142, 787
BcoDI GTCTC 1 cut(s) 452
BcuI ACTAGT 1 cut(s) 77
BfaI CTAG 1 cut(s) 78
BfmI CTRYAG 1 cut(s) 247
BfuI GTATCC 2 cut(s) 18, 544
BisI GCNGC 5 cut(s) 319, 398, 701, 741, 850
BlsI GCNGC 5 cut(s) 320, 399, 702, 742, 851
Bme1390I CCNGG 2 cut(s) 257, 498
BmeRI GACNNNNNGTC 1 cut(s) 690
BmgT120I GGNCC 3 cut(s) 253, 665, 711
BmiI GGNNCC 3 cut(s) 166, 254, 414
BmrFI CCNGG 2 cut(s) 257, 498
BmsI GCATC 4 cut(s) 184, 568, 574, 756
BpmI CTGGAG 1 cut(s) 573
Bpu10I CCTNAGC 1 cut(s) 362
BsaBI GATNNNNATC 1 cut(s) 627
BsaHI GRCGYC 2 cut(s) 691, 859
BsaJI CCNNGG 1 cut(s) 497
BsaXI ACNNNNNCTCC 4 cut(s) 507, 537, 883, 913
Bsc4I CCNNNNNNNGG 9 cut(s) 161, 197, 249, 250, 262, 396, 498, 727, 770
Bse1I ACTGG 3 cut(s) 106, 206, 484
Bse3DI GCAATG 2 cut(s) 155, 510
Bse8I GATNNNNATC 1 cut(s) 627
BseBI CCWGG 2 cut(s) 257, 498
BseDI CCNNGG 1 cut(s) 497
BseGI GGATG 2 cut(s) 206, 571
BseJI GATNNNNATC 1 cut(s) 627
BseLI CCNNNNNNNGG 9 cut(s) 161, 197, 249, 250, 262, 396, 498, 727, 770
BseMI GCAATG 2 cut(s) 155, 510
BseMII CTCAG 1 cut(s) 738
BseNI ACTGG 3 cut(s) 106, 206, 484
BseRI GAGGAG 1 cut(s) 907
BseXI GCAGC 3 cut(s) 305, 712, 727
BseYI CCCAGC 1 cut(s) 613
Bsh1236I CGCG 3 cut(s) 395, 397, 904
BshFI GGCC 6 cut(s) 105, 205, 255, 400, 667, 713
BsiHKAI GWGCWC 1 cut(s) 479
BsiSI CCGG 1 cut(s) 85
BslFI GGGAC 2 cut(s) 45, 894
BslI CCNNNNNNNGG 9 cut(s) 161, 197, 249, 250, 262, 396, 498, 727, 770
BsmAI GTCTC 1 cut(s) 452
BsmBI CGTCTC 1 cut(s) 452
BsmFI GGGAC 2 cut(s) 45, 894
BsmI GAATGC 1 cut(s) 88
BsnI GGCC 6 cut(s) 105, 205, 255, 400, 667, 713
Bsp1286I GDGCHC 1 cut(s) 479
Bsp143I GATC 4 cut(s) 142, 412, 466, 787
BspACI CCGC 4 cut(s) 393, 397, 728, 850
BspANI GGCC 6 cut(s) 105, 205, 255, 400, 667, 713
BspCNI CTCAG 1 cut(s) 737
BspFNI CGCG 3 cut(s) 395, 397, 904
BspHI TCATGA 1 cut(s) 43
BspLI GGNNCC 3 cut(s) 166, 254, 414
BspPI GGATC 2 cut(s) 407, 420
BsrDI GCAATG 2 cut(s) 155, 510
BsrI ACTGG 3 cut(s) 106, 206, 484
BssECI CCNNGG 1 cut(s) 497
BssMI GATC 4 cut(s) 142, 412, 466, 787
BssNI GRCGYC 2 cut(s) 691, 859
BssSI CACGAG 2 cut(s) 288, 402
Bst2BI CACGAG 2 cut(s) 288, 402
Bst2UI CCWGG 2 cut(s) 257, 498
Bst4CI ACNGT 3 cut(s) 98, 274, 827
Bst6I CTCTTC 2 cut(s) 555, 792
BstACI GRCGYC 2 cut(s) 691, 859
BstC8I GCNNGC 4 cut(s) 234, 238, 323, 745
BstDEI CTNAG 2 cut(s) 362, 724
BstF5I GGATG 2 cut(s) 206, 571
BstFNI CGCG 3 cut(s) 395, 397, 904
BstHHI GCGC 3 cut(s) 338, 397, 904
BstKTI GATC 4 cut(s) 145, 415, 469, 790
BstMAI GTCTC 1 cut(s) 452
BstMBI GATC 4 cut(s) 142, 412, 466, 787
BstMWI GCNNNNNNNGC 1 cut(s) 367
BstNI CCWGG 2 cut(s) 257, 498
BstNSI RCATGY 5 cut(s) 70, 240, 325, 374, 659
BstSCI CCNGG 2 cut(s) 255, 496
BstSFI CTRYAG 1 cut(s) 247
BstUI CGCG 3 cut(s) 395, 397, 904
BstV1I GCAGC 3 cut(s) 305, 712, 727
BstX2I RGATCY 1 cut(s) 412
BstYI RGATCY 1 cut(s) 412
BsuI GTATCC 2 cut(s) 18, 544
BsuRI GGCC 6 cut(s) 105, 205, 255, 400, 667, 713
BtsCI GGATG 2 cut(s) 206, 571
BtsI GCAGTG 1 cut(s) 742
BtsIMutI CAGTG 2 cut(s) 675, 742
Cac8I GCNNGC 4 cut(s) 234, 238, 323, 745
CaiI CAGNNNCTG 2 cut(s) 197, 677
CciI TCATGA 1 cut(s) 43
CfoI GCGC 3 cut(s) 338, 397, 904
Cfr13I GGNCC 3 cut(s) 253, 665, 711
CseI GACGC 1 cut(s) 848
Csp6I GTAC 3 cut(s) 109, 188, 209
CviQI GTAC 3 cut(s) 109, 188, 209
DdeI CTNAG 2 cut(s) 362, 724
DpnI GATC 4 cut(s) 144, 414, 468, 789
DpnII GATC 4 cut(s) 142, 412, 466, 787
DriI GACNNNNNGTC 1 cut(s) 690
EaeI YGGCCR 3 cut(s) 103, 203, 398
Eam1104I CTCTTC 2 cut(s) 555, 792
Eam1105I GACNNNNNGTC 1 cut(s) 690
EarI CTCTTC 2 cut(s) 555, 792
EcoO109I RGGNCCY 1 cut(s) 711
EcoRII CCWGG 2 cut(s) 255, 496
EcoT22I ATGCAT 1 cut(s) 661
Esp3I CGTCTC 1 cut(s) 452
FaqI GGGAC 2 cut(s) 45, 894
FbaI TGATCA 2 cut(s) 142, 787
FblI GTMKAC 2 cut(s) 488, 694
Fnu4HI GCNGC 5 cut(s) 319, 398, 701, 741, 850
FokI GGATG 2 cut(s) 213, 578
Fsp4HI GCNGC 5 cut(s) 319, 398, 701, 741, 850
FspBI CTAG 1 cut(s) 78
FspI TGCGCA 1 cut(s) 337
GlaI GCGC 3 cut(s) 337, 396, 903
GluI GCNGC 5 cut(s) 319, 398, 701, 741, 850
GsaI CCCAGC 1 cut(s) 617
GsuI CTGGAG 1 cut(s) 573
HaeIII GGCC 6 cut(s) 105, 205, 255, 400, 667, 713
HapII CCGG 1 cut(s) 85
HgaI GACGC 1 cut(s) 848
HhaI GCGC 3 cut(s) 338, 397, 904
Hin1I GRCGYC 2 cut(s) 691, 859
Hin6I GCGC 3 cut(s) 336, 395, 902
HinP1I GCGC 3 cut(s) 336, 395, 902
HindIII AAGCTT 1 cut(s) 117
HinfI GANTC 2 cut(s) 618, 779
HpaII CCGG 1 cut(s) 85
HphI GGTGA 4 cut(s) 271, 646, 827, 856
Hpy166II GTNNAC 3 cut(s) 489, 637, 695
Hpy188I TCNGA 4 cut(s) 142, 347, 471, 787
Hpy188III TCNNGA 6 cut(s) 44, 278, 416, 443, 590, 791
Hpy8I GTNNAC 3 cut(s) 489, 637, 695
Hpy99I CGWCG 1 cut(s) 827
HpyAV CCTTC 6 cut(s) 35, 256, 370, 724, 863, 905
HpyCH4III ACNGT 3 cut(s) 98, 274, 827
HpyCH4IV ACGT 3 cut(s) 639, 691, 883
HpyCH4V TGCA 5 cut(s) 236, 321, 325, 581, 659
HpyF10VI GCNNNNNNNGC 1 cut(s) 367
HpyF3I CTNAG 2 cut(s) 362, 724
HpySE526I ACGT 3 cut(s) 639, 691, 883
Hsp92I GRCGYC 2 cut(s) 691, 859
HspAI GCGC 3 cut(s) 336, 395, 902
Ksp22I TGATCA 2 cut(s) 142, 787
Kzo9I GATC 4 cut(s) 142, 412, 466, 787
LmnI GCTCC 3 cut(s) 170, 358, 592
Lsp1109I GCAGC 3 cut(s) 305, 712, 727
LweI GCATC 4 cut(s) 184, 568, 574, 756
MaeI CTAG 1 cut(s) 78
MaeII ACGT 3 cut(s) 639, 691, 883
MaeIII GTNAC 1 cut(s) 223
MalI GATC 4 cut(s) 144, 414, 468, 789
MboI GATC 4 cut(s) 142, 412, 466, 787
MboII GAAGA 4 cut(s) 30, 392, 572, 809
MflI RGATCY 1 cut(s) 412
MhlI GDGCHC 1 cut(s) 479
MlsI TGGCCA 2 cut(s) 105, 205
MluCI AATT 3 cut(s) 331, 349, 538
MluNI TGGCCA 2 cut(s) 105, 205
MmeI TCCRAC 4 cut(s) 117, 192, 203, 325
Mox20I TGGCCA 2 cut(s) 105, 205
Mph1103I ATGCAT 1 cut(s) 661
MscI TGGCCA 2 cut(s) 105, 205
MslI CAYNNNNRTG 1 cut(s) 453
Msp20I TGGCCA 2 cut(s) 105, 205
MspA1I CMGCKG 1 cut(s) 728
MspI CCGG 1 cut(s) 85
MspR9I CCNGG 2 cut(s) 257, 498
Mva1269I GAATGC 1 cut(s) 88
MvaI CCWGG 2 cut(s) 257, 498
MvnI CGCG 3 cut(s) 395, 397, 904
MwoI GCNNNNNNNGC 1 cut(s) 367
NdeII GATC 4 cut(s) 142, 412, 466, 787
NlaIV GGNNCC 3 cut(s) 166, 254, 414
NsbI TGCGCA 1 cut(s) 337
NsiI ATGCAT 1 cut(s) 661
NspI RCATGY 5 cut(s) 70, 240, 325, 374, 659
PaeI GCATGC 2 cut(s) 240, 325
PagI TCATGA 1 cut(s) 43
PciI ACATGT 1 cut(s) 66
PctI GAATGC 1 cut(s) 88
PfeI GAWTC 2 cut(s) 618, 779
PflMI CCANNNNNTGG 2 cut(s) 161, 197
PkrI GCNGC 5 cut(s) 320, 399, 702, 742, 851
PscI ACATGT 1 cut(s) 66
Psp6I CCWGG 2 cut(s) 255, 496
PspFI CCCAGC 1 cut(s) 613
PspGI CCWGG 2 cut(s) 255, 496
PspN4I GGNNCC 3 cut(s) 166, 254, 414
PspPI GGNCC 3 cut(s) 253, 665, 711
PstNI CAGNNNCTG 2 cut(s) 197, 677
PsuI RGATCY 1 cut(s) 412
RsaI GTAC 3 cut(s) 110, 189, 210
RsaNI GTAC 3 cut(s) 109, 188, 209
RseI CAYNNNNRTG 1 cut(s) 453
SatI GCNGC 5 cut(s) 319, 398, 701, 741, 850
Sau3AI GATC 4 cut(s) 142, 412, 466, 787
Sau96I GGNCC 3 cut(s) 253, 665, 711
ScrFI CCNGG 2 cut(s) 257, 498
SduI GDGCHC 1 cut(s) 479
SfaNI GCATC 4 cut(s) 184, 568, 574, 756
SfcI CTRYAG 1 cut(s) 247
SmiMI CAYNNNNRTG 1 cut(s) 453
SpeI ACTAGT 1 cut(s) 77
SphI GCATGC 2 cut(s) 240, 325
Sse9I AATT 3 cut(s) 331, 349, 538
SsiI CCGC 4 cut(s) 393, 397, 728, 850
SspMI CTAG 1 cut(s) 78
StyD4I CCNGG 2 cut(s) 255, 496
TaaI ACNGT 3 cut(s) 98, 274, 827
TaiI ACGT 3 cut(s) 642, 694, 886
TaqI TCGA 2 cut(s) 423, 621
TasI AATT 3 cut(s) 331, 349, 538
TatI WGTACW 2 cut(s) 108, 208
TauI GCSGC 2 cut(s) 400, 852
TfiI GAWTC 2 cut(s) 618, 779
TscAI CASTG 2 cut(s) 682, 742
TseI GCWGC 3 cut(s) 318, 700, 740
TspDTI ATGAA 2 cut(s) 810, 819
TspGWI ACGGA 1 cut(s) 569
TspRI CASTG 2 cut(s) 682, 742
Van91I CCANNNNNTGG 2 cut(s) 161, 197
XceI RCATGY 5 cut(s) 70, 240, 325, 374, 659
XcmI CCANNNNNNNNNTGG 1 cut(s) 198
XmiI GTMKAC 2 cut(s) 488, 694
XspI CTAG 1 cut(s) 78
ZraI GACGTC 1 cut(s) 692
Zsp2I ATGCAT 1 cut(s) 661
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.