MD08G1226800.v1.1

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0007434
Physical Location & Seq
Reverse (-)
29311344 .. 29314276
2933 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1226800.v1.1.491

Sequence Viewer

Length: 1107 bp
ATGATATTAGAAATGGCAAAAGGTAGCCGGGGACGACGCAGGATTGCTTCCCGACGATACAGACTGACTCCATACCCATTGCGGAACAGTCAAGATATATTGGAGGATCTGTGCTCGAAGAAATGCAGAGATTTGGAAAAAAAAGACTGGGAGGATGCAACATGTTCCGTGTGCATGGAGTATCCTCACAATGCTGTTCTTCTCCTTTGTTCTTCTCACGACAAAGGTTGCCGTCCCTACATGTGTGGAACTAGTTTCCGACATTCCAATTGTCTTGGCCAGTACAAGAAAGCTTATACTAAAATTGTGCCATCTGATCTTGGACAACCATTACTTGGCTCCAACAACGATCCAATTGTGGTACCAGATGCTGGATGGCCAGTACAAAAGTGTGAAGTCACAGAACTTGCATGCCCCCTCTGTAGGGGCCAGGTGAAGGGCTGGACTGTTCTTGAACCAGCACGAGATTATCTAAATACAAAAAGGAGAAGCTGCATGCAAGAAAATTGTTCATTTGTCGGAAATTACAAGGAGCTTAAAAGGCATGTGAAGGAAGAGCACCCCTCTGCACGCCCACGAGAAGTGGATCCAGACCTTGAACAGAAATGGAGAAGTCTTGAACATGAGCGTGAGACGAACGATCTGATGAGCACAATCCAGTCTTCTATGCCAGGTGCAATGGTTTTTGGAGATTATGTAATAGAAAGAAATAATTATGGTTTGGATACGGACGAAGAGGATGGTGGCTTTAATGCAGATGCTCCAGAAAGAAATGGGGGGTTCGGGCTGGGATTCGATGGCAATCTGGTGAACTTCTTTTTCCTATTACATGCATTTGGGCCATCAAGGACTGACCTGACAAGACGTCTAAGACCGCCTGAGGGGGCCTTCCACCACTCATTGGACAATAGTGCTGCTGGCATCCGGCACACAACTCCTGTTGGCGGTTTGGATTCCTCTGATCAAGATCAAGAGAATGATAGTGATGGTGATGATGACGGCGGTGGTATGTCATTGGCGAGCCGCCTTCGTCGTCATGGCAGGGTGTTGTTGGGACGTTCTGGGAGGAGACGTATGCGTAGAGAAGGAAACAGCGATGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

369

Amino Acids

41.56

Weight (kDa)

6.83

Isoelectric Point (pI)

60.58

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 51 - 217 8.7e-70 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 868
Acc65I GGTACC 1 cut(s) 361
AccB1I GGYRCC 1 cut(s) 361
AccB7I CCANNNNNTGG 3 cut(s) 335, 371, 901
AciI CCGC 5 cut(s) 82, 875, 945, 1002, 1024
AclWI GGATC 4 cut(s) 114, 344, 581, 594
AcoI YGGCCR 2 cut(s) 277, 377
AcyI GRCGYC 1 cut(s) 865
AfaI GTAC 3 cut(s) 284, 363, 384
AfiI CCNNNNNNNGG 8 cut(s) 335, 371, 423, 424, 436, 881, 901, 944
AflIII ACRYGT 2 cut(s) 161, 240
AgsI TTSAA 3 cut(s) 455, 599, 620
AhdI GACNNNNNGTC 1 cut(s) 864
AhlI ACTAGT 1 cut(s) 251
AjnI CCWGG 2 cut(s) 429, 670
AluBI AGCT 3 cut(s) 293, 492, 535
AluI AGCT 3 cut(s) 293, 492, 535
Alw21I GWGCWC 3 cut(s) 116, 561, 653
Alw26I GTCTC 2 cut(s) 626, 1063
AlwI GGATC 4 cut(s) 114, 344, 581, 594
AlwNI CAGNNNCTG 1 cut(s) 371
AoxI GGCC 5 cut(s) 277, 377, 427, 839, 885
ApeKI GCWGC 2 cut(s) 492, 914
Asp718I GGTACC 1 cut(s) 361
AspS9I GGNCC 3 cut(s) 427, 839, 885
AsuC2I CCSGG 1 cut(s) 29
AsuHPI GGTGA 3 cut(s) 445, 820, 1001
AxyI CCTNAGG 1 cut(s) 879
BalI TGGCCA 2 cut(s) 279, 379
BamHI GGATCC 1 cut(s) 586
BanI GGYRCC 1 cut(s) 361
BauI CACGAG 2 cut(s) 462, 576
BbsI GAAGAC 1 cut(s) 654
Bbv12I GWGCWC 3 cut(s) 116, 561, 653
BbvI GCAGC 2 cut(s) 479, 901
BccI CCATC 6 cut(s) 319, 369, 734, 791, 850, 980
BceAI ACGGC 2 cut(s) 216, 1015
BciT130I CCWGG 2 cut(s) 431, 672
BciVI GTATCC 2 cut(s) 192, 718
BclI TGATCA 1 cut(s) 961
BcnI CCSGG 1 cut(s) 29
BcoDI GTCTC 2 cut(s) 626, 1063
BcuI ACTAGT 1 cut(s) 251
BfaI CTAG 1 cut(s) 252
BfmI CTRYAG 1 cut(s) 421
BfuI GTATCC 2 cut(s) 192, 718
BisI GCNGC 3 cut(s) 493, 915, 1024
BlsI GCNGC 3 cut(s) 494, 916, 1025
Bme1390I CCNGG 3 cut(s) 29, 431, 672
BmeRI GACNNNNNGTC 1 cut(s) 864
BmgT120I GGNCC 3 cut(s) 427, 839, 885
BmiI GGNNCC 5 cut(s) 340, 363, 428, 588, 886
BmrFI CCNGG 3 cut(s) 29, 431, 672
BmrI ACTGGG 1 cut(s) 157
BmsI GCATC 4 cut(s) 145, 358, 748, 930
BmuI ACTGGG 1 cut(s) 157
BpiI GAAGAC 1 cut(s) 654
BplI GAGNNNNNCTC 2 cut(s) 548, 580
BpmI CTGGAG 1 cut(s) 747
BpuMI CCSGG 1 cut(s) 29
BsaBI GATNNNNATC 2 cut(s) 801, 966
BsaHI GRCGYC 1 cut(s) 865
BsaJI CCNNGG 1 cut(s) 28
BsaXI ACNNNNNCTCC 6 cut(s) 95, 125, 681, 711, 1057, 1087
Bsc4I CCNNNNNNNGG 8 cut(s) 335, 371, 423, 424, 436, 881, 901, 944
Bse1I ACTGG 4 cut(s) 152, 280, 380, 658
Bse21I CCTNAGG 1 cut(s) 879
Bse3DI GCAATG 2 cut(s) 77, 684
Bse8I GATNNNNATC 2 cut(s) 801, 966
BseBI CCWGG 2 cut(s) 431, 672
BseDI CCNNGG 1 cut(s) 28
BseGI GGATG 4 cut(s) 160, 380, 745, 921
BseJI GATNNNNATC 2 cut(s) 801, 966
BseLI CCNNNNNNNGG 8 cut(s) 335, 371, 423, 424, 436, 881, 901, 944
BseMI GCAATG 2 cut(s) 77, 684
BseMII CTCAG 1 cut(s) 870
BseNI ACTGG 4 cut(s) 152, 280, 380, 658
BseRI GAGGAG 1 cut(s) 1081
BseXI GCAGC 2 cut(s) 479, 901
BseYI CCCAGC 1 cut(s) 787
BsgI GTGCAG 1 cut(s) 552
BshFI GGCC 5 cut(s) 279, 379, 429, 841, 887
BshNI GGYRCC 1 cut(s) 361
BsiHKAI GWGCWC 3 cut(s) 116, 561, 653
BsiSI CCGG 2 cut(s) 28, 925
BslFI GGGAC 3 cut(s) 45, 219, 1068
BslI CCNNNNNNNGG 8 cut(s) 335, 371, 423, 424, 436, 881, 901, 944
BsmAI GTCTC 2 cut(s) 626, 1063
BsmBI CGTCTC 2 cut(s) 626, 1063
BsmFI GGGAC 3 cut(s) 45, 219, 1068
BsnI GGCC 5 cut(s) 279, 379, 429, 841, 887
Bsp1286I GDGCHC 3 cut(s) 116, 561, 653
Bsp143I GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
BspACI CCGC 5 cut(s) 82, 875, 945, 1002, 1024
BspANI GGCC 5 cut(s) 279, 379, 429, 841, 887
BspCNI CTCAG 1 cut(s) 871
BspLI GGNNCC 5 cut(s) 340, 363, 428, 588, 886
BspPI GGATC 4 cut(s) 114, 344, 581, 594
BspQI GCTCTTC 1 cut(s) 549
BspT107I GGYRCC 1 cut(s) 361
BsrDI GCAATG 2 cut(s) 77, 684
BsrI ACTGG 4 cut(s) 152, 280, 380, 658
BssECI CCNNGG 1 cut(s) 28
BssMI GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
BssNI GRCGYC 1 cut(s) 865
BssSI CACGAG 2 cut(s) 462, 576
Bst2BI CACGAG 2 cut(s) 462, 576
Bst2UI CCWGG 2 cut(s) 431, 672
Bst4CI ACNGT 2 cut(s) 89, 448
Bst6I CTCTTC 2 cut(s) 549, 729
BstACI GRCGYC 1 cut(s) 865
BstC8I GCNNGC 5 cut(s) 412, 497, 571, 919, 1021
BstDEI CTNAG 2 cut(s) 869, 879
BstF5I GGATG 4 cut(s) 160, 380, 745, 921
BstKTI GATC 7 cut(s) 109, 319, 352, 589, 643, 964, 970
BstMAI GTCTC 2 cut(s) 626, 1063
BstMBI GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
BstMWI GCNNNNNNNGC 1 cut(s) 541
BstNI CCWGG 2 cut(s) 431, 672
BstNSI RCATGY 6 cut(s) 165, 244, 414, 499, 548, 833
BstSCI CCNGG 3 cut(s) 27, 429, 670
BstSFI CTRYAG 1 cut(s) 421
BstV1I GCAGC 2 cut(s) 479, 901
BstV2I GAAGAC 1 cut(s) 654
BstX2I RGATCY 2 cut(s) 106, 586
BstYI RGATCY 2 cut(s) 106, 586
Bsu36I CCTNAGG 1 cut(s) 879
BsuI GTATCC 2 cut(s) 192, 718
BsuRI GGCC 5 cut(s) 279, 379, 429, 841, 887
BtsCI GGATG 4 cut(s) 160, 380, 745, 921
Cac8I GCNNGC 5 cut(s) 412, 497, 571, 919, 1021
CaiI CAGNNNCTG 1 cut(s) 371
Cfr13I GGNCC 3 cut(s) 427, 839, 885
CseI GACGC 1 cut(s) 45
Csp6I GTAC 3 cut(s) 283, 362, 383
CviAII CATG 9 cut(s) 162, 175, 241, 411, 496, 545, 623, 830, 1037
CviQI GTAC 3 cut(s) 283, 362, 383
DdeI CTNAG 2 cut(s) 869, 879
DpnI GATC 7 cut(s) 108, 318, 351, 588, 642, 963, 969
DpnII GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
DriI GACNNNNNGTC 1 cut(s) 864
EaeI YGGCCR 2 cut(s) 277, 377
Eam1104I CTCTTC 2 cut(s) 549, 729
Eam1105I GACNNNNNGTC 1 cut(s) 864
EarI CTCTTC 2 cut(s) 549, 729
Eco81I CCTNAGG 1 cut(s) 879
EcoO109I RGGNCCY 1 cut(s) 885
EcoRII CCWGG 2 cut(s) 429, 670
EcoT22I ATGCAT 1 cut(s) 835
Esp3I CGTCTC 2 cut(s) 626, 1063
FaeI CATG 9 cut(s) 165, 178, 244, 414, 499, 548, 626, 833, 1040
FaqI GGGAC 3 cut(s) 45, 219, 1068
FatI CATG 9 cut(s) 161, 174, 240, 410, 495, 544, 622, 829, 1036
FbaI TGATCA 1 cut(s) 961
Fnu4HI GCNGC 3 cut(s) 493, 915, 1024
FokI GGATG 4 cut(s) 167, 387, 752, 908
Fsp4HI GCNGC 3 cut(s) 493, 915, 1024
FspBI CTAG 1 cut(s) 252
GluI GCNGC 3 cut(s) 493, 915, 1024
GsaI CCCAGC 1 cut(s) 791
GsuI CTGGAG 1 cut(s) 747
HaeIII GGCC 5 cut(s) 279, 379, 429, 841, 887
HapII CCGG 2 cut(s) 28, 925
HgaI GACGC 1 cut(s) 45
Hin1I GRCGYC 1 cut(s) 865
Hin1II CATG 9 cut(s) 165, 178, 244, 414, 499, 548, 626, 833, 1040
HindIII AAGCTT 1 cut(s) 291
HinfI GANTC 3 cut(s) 67, 792, 953
HpaII CCGG 2 cut(s) 28, 925
HphI GGTGA 3 cut(s) 445, 820, 1001
Hpy166II GTNNAC 1 cut(s) 811
Hpy188I TCNGA 5 cut(s) 260, 316, 521, 645, 961
Hpy188III TCNNGA 9 cut(s) 51, 92, 218, 452, 590, 617, 764, 965, 971
Hpy8I GTNNAC 1 cut(s) 811
Hpy99I CGWCG 3 cut(s) 39, 57, 1035
HpyAV CCTTC 5 cut(s) 430, 544, 898, 1037, 1079
HpyCH4III ACNGT 2 cut(s) 89, 448
HpyCH4IV ACGT 3 cut(s) 865, 1057, 1072
HpyF10VI GCNNNNNNNGC 1 cut(s) 541
HpyF3I CTNAG 2 cut(s) 869, 879
HpySE526I ACGT 3 cut(s) 865, 1057, 1072
Hsp92I GRCGYC 1 cut(s) 865
Hsp92II CATG 9 cut(s) 165, 178, 244, 414, 499, 548, 626, 833, 1040
KpnI GGTACC 1 cut(s) 365
Ksp22I TGATCA 1 cut(s) 961
Kzo9I GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
LguI GCTCTTC 1 cut(s) 549
LmnI GCTCC 3 cut(s) 344, 532, 766
Lsp1109I GCAGC 2 cut(s) 479, 901
LweI GCATC 4 cut(s) 145, 358, 748, 930
MaeI CTAG 1 cut(s) 252
MaeII ACGT 3 cut(s) 865, 1057, 1072
MaeIII GTNAC 1 cut(s) 397
MalI GATC 7 cut(s) 108, 318, 351, 588, 642, 963, 969
MboI GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
MboII GAAGA 6 cut(s) 130, 191, 204, 566, 654, 746
MfeI CAATTG 2 cut(s) 268, 354
MflI RGATCY 2 cut(s) 106, 586
MhlI GDGCHC 3 cut(s) 116, 561, 653
MlsI TGGCCA 2 cut(s) 279, 379
MluCI AATT 6 cut(s) 268, 303, 354, 505, 523, 712
MluNI TGGCCA 2 cut(s) 279, 379
MlyI GAGTC 1 cut(s) 61
MmeI TCCRAC 3 cut(s) 283, 366, 499
MnlI CCTC 9 cut(s) 97, 145, 195, 428, 574, 730, 874, 967, 1059
Mox20I TGGCCA 2 cut(s) 279, 379
Mph1103I ATGCAT 1 cut(s) 835
MscI TGGCCA 2 cut(s) 279, 379
MseI TTAA 2 cut(s) 537, 750
MslI CAYNNNNRTG 1 cut(s) 627
Msp20I TGGCCA 2 cut(s) 279, 379
MspI CCGG 2 cut(s) 28, 925
MspR9I CCNGG 3 cut(s) 29, 431, 672
MunI CAATTG 2 cut(s) 268, 354
MvaI CCWGG 2 cut(s) 431, 672
MwoI GCNNNNNNNGC 1 cut(s) 541
NciI CCSGG 1 cut(s) 29
NdeII GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
NlaIII CATG 9 cut(s) 165, 178, 244, 414, 499, 548, 626, 833, 1040
NlaIV GGNNCC 5 cut(s) 340, 363, 428, 588, 886
NmuCI GTSAC 1 cut(s) 397
NsiI ATGCAT 1 cut(s) 835
NspI RCATGY 6 cut(s) 165, 244, 414, 499, 548, 833
PaeI GCATGC 2 cut(s) 414, 499
PciI ACATGT 2 cut(s) 161, 240
PciSI GCTCTTC 1 cut(s) 549
PfeI GAWTC 2 cut(s) 792, 953
PflMI CCANNNNNTGG 3 cut(s) 335, 371, 901
PkrI GCNGC 3 cut(s) 494, 916, 1025
PleI GAGTC 1 cut(s) 61
PpsI GAGTC 1 cut(s) 61
PscI ACATGT 2 cut(s) 161, 240
Psp6I CCWGG 2 cut(s) 429, 670
PspFI CCCAGC 1 cut(s) 787
PspGI CCWGG 2 cut(s) 429, 670
PspN4I GGNNCC 5 cut(s) 340, 363, 428, 588, 886
PspPI GGNCC 3 cut(s) 427, 839, 885
PstNI CAGNNNCTG 1 cut(s) 371
PsuI RGATCY 2 cut(s) 106, 586
RsaI GTAC 3 cut(s) 284, 363, 384
RsaNI GTAC 3 cut(s) 283, 362, 383
RseI CAYNNNNRTG 1 cut(s) 627
SapI GCTCTTC 1 cut(s) 549
SaqAI TTAA 2 cut(s) 537, 750
SatI GCNGC 3 cut(s) 493, 915, 1024
Sau3AI GATC 7 cut(s) 106, 316, 349, 586, 640, 961, 967
Sau96I GGNCC 3 cut(s) 427, 839, 885
SchI GAGTC 1 cut(s) 61
ScrFI CCNGG 3 cut(s) 29, 431, 672
SduI GDGCHC 3 cut(s) 116, 561, 653
SfaNI GCATC 4 cut(s) 145, 358, 748, 930
SfcI CTRYAG 1 cut(s) 421
SmiMI CAYNNNNRTG 1 cut(s) 627
SpeI ACTAGT 1 cut(s) 251
SphI GCATGC 2 cut(s) 414, 499
Sse9I AATT 6 cut(s) 268, 303, 354, 505, 523, 712
SsiI CCGC 5 cut(s) 82, 875, 945, 1002, 1024
SspMI CTAG 1 cut(s) 252
StyD4I CCNGG 3 cut(s) 27, 429, 670
TaaI ACNGT 2 cut(s) 89, 448
TaiI ACGT 3 cut(s) 868, 1060, 1075
TaqI TCGA 2 cut(s) 116, 795
TasI AATT 6 cut(s) 268, 303, 354, 505, 523, 712
TatI WGTACW 2 cut(s) 282, 382
TauI GCSGC 1 cut(s) 1026
TfiI GAWTC 2 cut(s) 792, 953
Tru1I TTAA 2 cut(s) 537, 750
Tru9I TTAA 2 cut(s) 537, 750
TseFI GTSAC 1 cut(s) 397
TseI GCWGC 2 cut(s) 492, 914
Tsp45I GTSAC 1 cut(s) 397
TspDTI ATGAA 1 cut(s) 501
TspGWI ACGGA 2 cut(s) 157, 743
Van91I CCANNNNNTGG 3 cut(s) 335, 371, 901
XceI RCATGY 6 cut(s) 165, 244, 414, 499, 548, 833
XcmI CCANNNNNNNNNTGG 1 cut(s) 372
XspI CTAG 1 cut(s) 252
ZraI GACGTC 1 cut(s) 866
Zsp2I ATGCAT 1 cut(s) 835
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.