pycom15g37200

Protein of unknown function (DUF1644)

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr15
OG0007434
Physical Location & Seq
Reverse (-)
37093159 .. 37094112
954 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom15g37200.1

Sequence Viewer

Length: 954 bp
ATGCAACATGTTCTGTGTGCATGGAGTATCCTCACAATGCTGTCCTTCTCCTTTGTTCTTCTCATGACAAAGGTTGCCGTCCCTACATGTGTGGAACTAGTTTTCGGCATTCCAACTGTCTTGGCCAGTACAAGAAAGCTTATACTAAAATGGGGTCAGCTCGATCATGGACAACCATTGCTTGGCTCCAACAACAATCCAACTGCGGTACCAGATGCTGGATGGCCAGTACACAAGTGTGAAGTCACAGAGCTTGCATGCCCCCTCTGTAGGGGCCAGGTGAAGGGCTGGACTGTTCTTGAACCAGCACGAGATTATCTAAATACAAAAAGGAGAAGCTGCATGCAAGAAAATTGCTCATTTGTCGGAAATTACAAGGAGCTTAGGAGGCATGTGAAGGAAGAACACCCCTCCGCGCGGCCACGAGAAGTGGATCCAGACCTCGAACAGAAATGGAGAAGTCTTGAGCATGAGCGTGAGACGAACGATCTGATGAGCACAATCCAGTCTACCATGCCAGGGGCAATGGTTTTTGGAGATTATGTAATAGAAAGAAATAATTATGGTTTGGATACAGACGAAGAGGATGGTGGCTTTGATGCAGATGCTCCCGAAAGAAATGGGGGGTTCGGGCTGGGATTCGATGGCAATCTGGTGAACGTCTTTTTCCTATTACATGCATTTGGGCCACCAGGCACTGACCTGACAAGACGTCTACGGCAGACAGAGAGGGCCTTCCACCACTCAGCGGATGGCAGTGCTGCTGGCATCAGGCACACAACTCCTATTGGAGGTTTAGATTCCTCTGATCAAGATGAAGAGAATGAAAGTGATGGTGACGATGACGGTGGTGGTATGTCGTTGGTGAGCCGCCTTCGGCGTCATGGGAGGGTGTTGTTGGGACGTTCTGGGAGGAGAGGTGCGCGTAGAGAAGGAAATAGTGATGAAAGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF1644

Protein Analysis

318

Amino Acids

35.24

Weight (kDa)

5.69

Isoelectric Point (pI)

51.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF1644 PF07800 79 - 166 3.3e-39 Protein of unknown function (DUF1644)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 715
Acc65I GGTACC 1 cut(s) 208
AccB1I GGYRCC 1 cut(s) 208
AccB7I CCANNNNNTGG 2 cut(s) 182, 218
AccI GTMKAC 2 cut(s) 509, 715
AccII CGCG 3 cut(s) 416, 418, 925
AciI CCGC 5 cut(s) 206, 414, 418, 749, 871
AclWI GGATC 2 cut(s) 428, 441
AcoI YGGCCR 3 cut(s) 123, 224, 419
AcyI GRCGYC 2 cut(s) 712, 880
AfaI GTAC 3 cut(s) 130, 210, 231
AfiI CCNNNNNNNGG 9 cut(s) 182, 218, 270, 271, 283, 417, 519, 748, 791
AflIII ACRYGT 2 cut(s) 7, 86
AgsI TTSAA 1 cut(s) 302
AhdI GACNNNNNGTC 1 cut(s) 711
AhlI ACTAGT 1 cut(s) 97
AjnI CCWGG 3 cut(s) 276, 517, 691
AleI CACNNNNGTG 1 cut(s) 237
AluBI AGCT 5 cut(s) 139, 160, 253, 339, 382
AluI AGCT 5 cut(s) 139, 160, 253, 339, 382
Alw21I GWGCWC 1 cut(s) 500
Alw26I GTCTC 1 cut(s) 473
AlwI GGATC 2 cut(s) 428, 441
AlwNI CAGNNNCTG 2 cut(s) 218, 698
AoxI GGCC 6 cut(s) 123, 224, 274, 419, 686, 732
ApeKI GCWGC 2 cut(s) 339, 761
Asp718I GGTACC 1 cut(s) 208
AspLEI GCGC 2 cut(s) 418, 925
AspS9I GGNCC 3 cut(s) 274, 686, 732
AsuHPI GGTGA 4 cut(s) 292, 667, 848, 877
BaeI ACNNNNGTAYC 2 cut(s) 192, 225
BalI TGGCCA 2 cut(s) 125, 226
BamHI GGATCC 1 cut(s) 433
BanI GGYRCC 1 cut(s) 208
BauI CACGAG 2 cut(s) 309, 423
Bbv12I GWGCWC 1 cut(s) 500
BbvI GCAGC 2 cut(s) 326, 748
BccI CCATC 5 cut(s) 216, 581, 638, 746, 827
BceAI ACGGC 2 cut(s) 62, 734
BciT130I CCWGG 3 cut(s) 278, 519, 693
BciVI GTATCC 2 cut(s) 38, 565
BclI TGATCA 1 cut(s) 808
BcoDI GTCTC 1 cut(s) 473
BcuI ACTAGT 1 cut(s) 97
BfaI CTAG 1 cut(s) 98
BfmI CTRYAG 1 cut(s) 268
BfuI GTATCC 2 cut(s) 38, 565
BisI GCNGC 4 cut(s) 340, 419, 762, 871
BlsI GCNGC 4 cut(s) 341, 420, 763, 872
Bme1390I CCNGG 3 cut(s) 278, 519, 693
BmeRI GACNNNNNGTC 1 cut(s) 711
BmgT120I GGNCC 3 cut(s) 274, 686, 732
BmiI GGNNCC 4 cut(s) 187, 210, 275, 435
BmrFI CCNGG 3 cut(s) 278, 519, 693
BmsI GCATC 4 cut(s) 205, 589, 595, 777
Bpu10I CCTNAGC 1 cut(s) 383
BpuEI CTTGAG 1 cut(s) 485
BsaBI GATNNNNATC 1 cut(s) 648
BsaHI GRCGYC 2 cut(s) 712, 880
BsaJI CCNNGG 1 cut(s) 518
BsaXI ACNNNNNCTCC 4 cut(s) 528, 558, 904, 934
Bsc4I CCNNNNNNNGG 9 cut(s) 182, 218, 270, 271, 283, 417, 519, 748, 791
Bse1I ACTGG 3 cut(s) 126, 227, 505
Bse3DI GCAATG 2 cut(s) 176, 531
Bse8I GATNNNNATC 1 cut(s) 648
BseBI CCWGG 3 cut(s) 278, 519, 693
BseDI CCNNGG 1 cut(s) 518
BseGI GGATG 3 cut(s) 227, 592, 757
BseJI GATNNNNATC 1 cut(s) 648
BseLI CCNNNNNNNGG 9 cut(s) 182, 218, 270, 271, 283, 417, 519, 748, 791
BseMI GCAATG 2 cut(s) 176, 531
BseMII CTCAG 1 cut(s) 759
BseNI ACTGG 3 cut(s) 126, 227, 505
BseRI GAGGAG 1 cut(s) 928
BseXI GCAGC 2 cut(s) 326, 748
BseYI CCCAGC 1 cut(s) 634
Bsh1236I CGCG 3 cut(s) 416, 418, 925
BshFI GGCC 6 cut(s) 125, 226, 276, 421, 688, 734
BshNI GGYRCC 1 cut(s) 208
BsiHKAI GWGCWC 1 cut(s) 500
BslFI GGGAC 2 cut(s) 65, 915
BslI CCNNNNNNNGG 9 cut(s) 182, 218, 270, 271, 283, 417, 519, 748, 791
BsmAI GTCTC 1 cut(s) 473
BsmBI CGTCTC 1 cut(s) 473
BsmFI GGGAC 2 cut(s) 65, 915
BsmI GAATGC 1 cut(s) 108
BsnI GGCC 6 cut(s) 125, 226, 276, 421, 688, 734
Bsp1286I GDGCHC 1 cut(s) 500
Bsp143I GATC 4 cut(s) 163, 433, 487, 808
BspACI CCGC 5 cut(s) 206, 414, 418, 749, 871
BspANI GGCC 6 cut(s) 125, 226, 276, 421, 688, 734
BspCNI CTCAG 1 cut(s) 758
BspFNI CGCG 3 cut(s) 416, 418, 925
BspHI TCATGA 1 cut(s) 63
BspLI GGNNCC 4 cut(s) 187, 210, 275, 435
BspPI GGATC 2 cut(s) 428, 441
BspT107I GGYRCC 1 cut(s) 208
BsrDI GCAATG 2 cut(s) 176, 531
BsrI ACTGG 3 cut(s) 126, 227, 505
BssECI CCNNGG 1 cut(s) 518
BssMI GATC 4 cut(s) 163, 433, 487, 808
BssNI GRCGYC 2 cut(s) 712, 880
BssSI CACGAG 2 cut(s) 309, 423
Bst2BI CACGAG 2 cut(s) 309, 423
Bst2UI CCWGG 3 cut(s) 278, 519, 693
Bst4CI ACNGT 3 cut(s) 118, 295, 848
Bst6I CTCTTC 2 cut(s) 576, 813
BstACI GRCGYC 2 cut(s) 712, 880
BstC8I GCNNGC 4 cut(s) 255, 259, 344, 766
BstDEI CTNAG 2 cut(s) 383, 745
BstENI CCTNNNNNAGG 1 cut(s) 789
BstF5I GGATG 3 cut(s) 227, 592, 757
BstFNI CGCG 3 cut(s) 416, 418, 925
BstHHI GCGC 2 cut(s) 418, 925
BstKTI GATC 4 cut(s) 166, 436, 490, 811
BstMAI GTCTC 1 cut(s) 473
BstMBI GATC 4 cut(s) 163, 433, 487, 808
BstMWI GCNNNNNNNGC 1 cut(s) 388
BstNI CCWGG 3 cut(s) 278, 519, 693
BstNSI RCATGY 6 cut(s) 11, 90, 261, 346, 395, 680
BstSCI CCNGG 3 cut(s) 276, 517, 691
BstSFI CTRYAG 1 cut(s) 268
BstUI CGCG 3 cut(s) 416, 418, 925
BstV1I GCAGC 2 cut(s) 326, 748
BstX2I RGATCY 1 cut(s) 433
BstYI RGATCY 1 cut(s) 433
BsuI GTATCC 2 cut(s) 38, 565
BsuRI GGCC 6 cut(s) 125, 226, 276, 421, 688, 734
BtsCI GGATG 3 cut(s) 227, 592, 757
BtsI GCAGTG 1 cut(s) 763
BtsIMutI CAGTG 2 cut(s) 696, 763
Cac8I GCNNGC 4 cut(s) 255, 259, 344, 766
CaiI CAGNNNCTG 2 cut(s) 218, 698
CciI TCATGA 1 cut(s) 63
CfoI GCGC 2 cut(s) 418, 925
Cfr13I GGNCC 3 cut(s) 274, 686, 732
CseI GACGC 1 cut(s) 869
Csp6I GTAC 3 cut(s) 129, 209, 230
CviQI GTAC 3 cut(s) 129, 209, 230
DdeI CTNAG 2 cut(s) 383, 745
DpnI GATC 4 cut(s) 165, 435, 489, 810
DpnII GATC 4 cut(s) 163, 433, 487, 808
DriI GACNNNNNGTC 1 cut(s) 711
EaeI YGGCCR 3 cut(s) 123, 224, 419
Eam1104I CTCTTC 2 cut(s) 576, 813
Eam1105I GACNNNNNGTC 1 cut(s) 711
EarI CTCTTC 2 cut(s) 576, 813
EcoNI CCTNNNNNAGG 1 cut(s) 789
EcoO109I RGGNCCY 1 cut(s) 732
EcoRII CCWGG 3 cut(s) 276, 517, 691
EcoT22I ATGCAT 1 cut(s) 682
Esp3I CGTCTC 1 cut(s) 473
FaqI GGGAC 2 cut(s) 65, 915
FbaI TGATCA 1 cut(s) 808
FblI GTMKAC 2 cut(s) 509, 715
Fnu4HI GCNGC 4 cut(s) 340, 419, 762, 871
FokI GGATG 3 cut(s) 234, 599, 764
Fsp4HI GCNGC 4 cut(s) 340, 419, 762, 871
FspBI CTAG 1 cut(s) 98
GlaI GCGC 2 cut(s) 417, 924
GluI GCNGC 4 cut(s) 340, 419, 762, 871
GsaI CCCAGC 1 cut(s) 638
HaeIII GGCC 6 cut(s) 125, 226, 276, 421, 688, 734
HgaI GACGC 1 cut(s) 869
HhaI GCGC 2 cut(s) 418, 925
Hin1I GRCGYC 2 cut(s) 712, 880
Hin6I GCGC 2 cut(s) 416, 923
HinP1I GCGC 2 cut(s) 416, 923
HindIII AAGCTT 1 cut(s) 137
HinfI GANTC 2 cut(s) 639, 800
HphI GGTGA 4 cut(s) 292, 667, 848, 877
Hpy166II GTNNAC 4 cut(s) 232, 510, 658, 716
Hpy188I TCNGA 3 cut(s) 368, 492, 808
Hpy188III TCNNGA 6 cut(s) 64, 299, 437, 464, 611, 812
Hpy8I GTNNAC 4 cut(s) 232, 510, 658, 716
HpyAV CCTTC 6 cut(s) 55, 277, 391, 745, 884, 926
HpyCH4III ACNGT 3 cut(s) 118, 295, 848
HpyCH4IV ACGT 3 cut(s) 660, 712, 904
HpyCH4V TGCA 7 cut(s) 4, 20, 257, 342, 346, 602, 680
HpyF10VI GCNNNNNNNGC 1 cut(s) 388
HpyF3I CTNAG 2 cut(s) 383, 745
HpySE526I ACGT 3 cut(s) 660, 712, 904
Hsp92I GRCGYC 2 cut(s) 712, 880
HspAI GCGC 2 cut(s) 416, 923
KpnI GGTACC 1 cut(s) 212
Ksp22I TGATCA 1 cut(s) 808
Kzo9I GATC 4 cut(s) 163, 433, 487, 808
LmnI GCTCC 3 cut(s) 191, 379, 613
Lsp1109I GCAGC 2 cut(s) 326, 748
LweI GCATC 4 cut(s) 205, 589, 595, 777
MaeI CTAG 1 cut(s) 98
MaeII ACGT 3 cut(s) 660, 712, 904
MaeIII GTNAC 2 cut(s) 244, 836
MalI GATC 4 cut(s) 165, 435, 489, 810
MboI GATC 4 cut(s) 163, 433, 487, 808
MboII GAAGA 4 cut(s) 50, 413, 593, 830
MflI RGATCY 1 cut(s) 433
MhlI GDGCHC 1 cut(s) 500
MlsI TGGCCA 2 cut(s) 125, 226
MluCI AATT 3 cut(s) 352, 370, 559
MluNI TGGCCA 2 cut(s) 125, 226
MmeI TCCRAC 4 cut(s) 137, 213, 224, 346
Mox20I TGGCCA 2 cut(s) 125, 226
Mph1103I ATGCAT 1 cut(s) 682
MscI TGGCCA 2 cut(s) 125, 226
MslI CAYNNNNRTG 2 cut(s) 237, 474
Msp20I TGGCCA 2 cut(s) 125, 226
MspA1I CMGCKG 1 cut(s) 749
MspR9I CCNGG 3 cut(s) 278, 519, 693
Mva1269I GAATGC 1 cut(s) 108
MvaI CCWGG 3 cut(s) 278, 519, 693
MvnI CGCG 3 cut(s) 416, 418, 925
MwoI GCNNNNNNNGC 1 cut(s) 388
NdeII GATC 4 cut(s) 163, 433, 487, 808
NlaIV GGNNCC 4 cut(s) 187, 210, 275, 435
NmuCI GTSAC 2 cut(s) 244, 836
NsiI ATGCAT 1 cut(s) 682
NspI RCATGY 6 cut(s) 11, 90, 261, 346, 395, 680
OliI CACNNNNGTG 1 cut(s) 237
PaeI GCATGC 2 cut(s) 261, 346
PagI TCATGA 1 cut(s) 63
PciI ACATGT 2 cut(s) 7, 86
PctI GAATGC 1 cut(s) 108
PfeI GAWTC 2 cut(s) 639, 800
PflMI CCANNNNNTGG 2 cut(s) 182, 218
PkrI GCNGC 4 cut(s) 341, 420, 763, 872
PscI ACATGT 2 cut(s) 7, 86
Psp6I CCWGG 3 cut(s) 276, 517, 691
PspFI CCCAGC 1 cut(s) 634
PspGI CCWGG 3 cut(s) 276, 517, 691
PspN4I GGNNCC 4 cut(s) 187, 210, 275, 435
PspPI GGNCC 3 cut(s) 274, 686, 732
PstNI CAGNNNCTG 2 cut(s) 218, 698
PsuI RGATCY 1 cut(s) 433
RsaI GTAC 3 cut(s) 130, 210, 231
RsaNI GTAC 3 cut(s) 129, 209, 230
RseI CAYNNNNRTG 2 cut(s) 237, 474
SatI GCNGC 4 cut(s) 340, 419, 762, 871
Sau3AI GATC 4 cut(s) 163, 433, 487, 808
Sau96I GGNCC 3 cut(s) 274, 686, 732
ScrFI CCNGG 3 cut(s) 278, 519, 693
SduI GDGCHC 1 cut(s) 500
SfaNI GCATC 4 cut(s) 205, 589, 595, 777
SfcI CTRYAG 1 cut(s) 268
SmiMI CAYNNNNRTG 2 cut(s) 237, 474
SmlI CTYRAG 1 cut(s) 464
SmoI CTYRAG 1 cut(s) 464
SpeI ACTAGT 1 cut(s) 97
SphI GCATGC 2 cut(s) 261, 346
Sse9I AATT 3 cut(s) 352, 370, 559
SsiI CCGC 5 cut(s) 206, 414, 418, 749, 871
SspMI CTAG 1 cut(s) 98
StyD4I CCNGG 3 cut(s) 276, 517, 691
TaaI ACNGT 3 cut(s) 118, 295, 848
TaiI ACGT 3 cut(s) 663, 715, 907
TaqI TCGA 3 cut(s) 162, 444, 642
TasI AATT 3 cut(s) 352, 370, 559
TatI WGTACW 2 cut(s) 128, 229
TauI GCSGC 2 cut(s) 421, 873
TfiI GAWTC 2 cut(s) 639, 800
TscAI CASTG 2 cut(s) 703, 763
TseFI GTSAC 2 cut(s) 244, 836
TseI GCWGC 2 cut(s) 339, 761
Tsp45I GTSAC 2 cut(s) 244, 836
TspDTI ATGAA 2 cut(s) 831, 840
TspRI CASTG 2 cut(s) 703, 763
Van91I CCANNNNNTGG 2 cut(s) 182, 218
XagI CCTNNNNNAGG 1 cut(s) 789
XceI RCATGY 6 cut(s) 11, 90, 261, 346, 395, 680
XcmI CCANNNNNNNNNTGG 2 cut(s) 219, 749
XmiI GTMKAC 2 cut(s) 509, 715
XspI CTAG 1 cut(s) 98
ZraI GACGTC 1 cut(s) 713
Zsp2I ATGCAT 1 cut(s) 682
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.