FvH4_5g30870

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005100
Physical Location & Seq
Reverse (-)
21731767 .. 21735235
3469 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g30870.t2

Sequence Viewer

Length: 1326 bp
ATGGACCCTGTTGAAGTTGGTCTGGGTTGTGGGAGTGACACAGCTCACCTTTCTCTAATGGGTTACAATCCCAGGGTATATTACCGGGGCCGAGGTGCATTTGAGTACATGGGAGCAGGATTGGCAATGTCACCAGGTGATATTGCATTTAAGTCTAATTTTGCAACATTGGATGAAAAAACTGGAATTGTCATCAGTAGGAGGGCTGACAGACACTTTGAAGAAGAAGGGCCCATACTGTGTGCAGCACTCGATGGATTGAAGTTGCCATCTTTTCCCGAATATGAAGTCAGAGTCAGGTATGCAACAGAACATAGATGTGGAGTGGTTGTAAAAGGACCTAATTTAAGTGGAAACATATCGGGAATAGACCCATTGAAGGATAACCGTTTACTTTTGCAAGCTGAAGCTCTAGATGGTAGTGATGAGGCAAGGCATACAGCTAAAGTTGTTAACGAGTTATCCAAGGAAATATCAAAGATTCTCATTGCTCATCCGCTGAATGCAAAACGGGCCTCTGAAGGGAAGAACATAGCTAATGTGGTCCTATTACGAGGTTGTGGTATCCGGATTGAGGTTTCTCCATTTGAAAAGAAACATGGGTTATGGCCTTGTATGGTAGCTCCAACAAAAATCATTGCTGGATTGGGTTTATCCCTTGGCATTGATATCCTGGAAGCTCCTGGAGCAACTGGAGATTACAGAACACTTCTAACTTCCAAAGCAACTGCAATAGCCAAGGCACTTTCAGCTCCACTGCAGTCTTGCCCCAATGTGTTTGTGCCAGGGGAAGATGAACACAAACCAGGCCGATCAGATGGCTATGATTTTGGGTTCCTCCACATAAAGGCTATAGATGATGCAGGTCATGATAAGGCAACCATTTTCAAAGTCAAAGGATTAGAAGCTGTAGATCGAGCTATAGGGCAGCTGGCCAGGCTCCTTTGGGAGACTGAATCAACAGGAAAATTTCAGTACTTCCTTTGTGTTACAGGAGACCACTCTACCCCAGTTGAATATGGAGACCACAGCTTTGAGCCAGTTCCATTCACAATATGTTCATTGAAAGATTTTGTTGGTGCTGTAGGTGCGGAAACGGTTATGGGTGCTTCTCTTGATCCATTTCCTCTTCCAACCGTAAAAGAGGGTGAATATCCAACAGAGCATGTGGAGATGGCACAGGAGCCTACCAAACAGCCTCAAGCTTTCGACAGTGATTCAGTTTGTGAGTTTAATGAGATAGCGGCAGCAAGGGGATGTCTTGGACGTTTTCCTGGCGGTGAGATGATGGGAGTAATAAAGAATTTCCTGGAGCTAGAAGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metalloenzyme PhosphMutase

Protein Analysis

442

Amino Acids

47.58

Weight (kDa)

5.34

Isoelectric Point (pI)

33.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PhosphMutase PF10143 1 - 168 2.6e-47 2,3-bisphosphoglycerate-independent phosphoglycerate mutase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 854
AccIII TCCGGA 1 cut(s) 567
AciI CCGC 4 cut(s) 497, 1091, 1244, 1278
AclWI GGATC 1 cut(s) 1112
AcoI YGGCCR 1 cut(s) 933
AcsI RAATTY 2 cut(s) 968, 1303
AcuI CTGAAG 2 cut(s) 426, 540
AdeI CACNNNGTG 1 cut(s) 137
AfaI GTAC 2 cut(s) 107, 977
AfiI CCNNNNNNNGG 4 cut(s) 379, 522, 574, 847
AgsI TTSAA 8 cut(s) 14, 221, 262, 379, 590, 889, 1016, 1066
AjnI CCWGG 9 cut(s) 71, 133, 672, 682, 784, 805, 935, 1273, 1308
Alw26I GTCTC 3 cut(s) 944, 990, 1017
AlwI GGATC 1 cut(s) 1112
Aor13HI TCCGGA 1 cut(s) 567
AoxI GGCC 6 cut(s) 88, 230, 513, 608, 808, 933
ApaI GGGCCC 1 cut(s) 234
ApeKI GCWGC 3 cut(s) 245, 928, 1247
ApoI RAATTY 2 cut(s) 968, 1303
ArsI GACNNNNNNTTYG 6 cut(s) 200, 232, 273, 305, 1016, 1048
AspS9I GGNCC 7 cut(s) 4, 88, 230, 231, 338, 513, 544
AsuC2I CCSGG 1 cut(s) 86
AsuHPI GGTGA 5 cut(s) 38, 123, 149, 1160, 1292
AvaII GGWCC 3 cut(s) 4, 338, 544
BaeGI GKGCMC 1 cut(s) 234
BalI TGGCCA 1 cut(s) 935
BanII GRGCYC 1 cut(s) 234
BarI GAAGNNNNNNTAC 2 cut(s) 219, 251
BbvI GCAGC 3 cut(s) 257, 940, 1259
BccI CCATC 6 cut(s) 248, 277, 410, 812, 1168, 1282
BciT130I CCWGG 9 cut(s) 73, 135, 674, 684, 786, 807, 937, 1275, 1310
BciVI GTATCC 1 cut(s) 575
BcnI CCSGG 1 cut(s) 86
BcoDI GTCTC 3 cut(s) 944, 990, 1017
BfaI CTAG 2 cut(s) 413, 1316
BfmI CTRYAG 5 cut(s) 758, 852, 909, 921, 1083
BfuAI ACCTGC 1 cut(s) 854
BfuI GTATCC 1 cut(s) 575
BisI GCNGC 4 cut(s) 246, 929, 1245, 1248
BlsI GCNGC 4 cut(s) 247, 930, 1246, 1249
BmcAI AGTACT 1 cut(s) 977
Bme18I GGWCC 3 cut(s) 4, 338, 544
BmgT120I GGNCC 7 cut(s) 4, 88, 230, 231, 338, 513, 544
BmiI GGNNCC 6 cut(s) 6, 89, 232, 836, 941, 1185
BmrI ACTGGG 1 cut(s) 1004
BmsI GCATC 1 cut(s) 850
BmuI ACTGGG 1 cut(s) 1004
BpmI CTGGAG 2 cut(s) 705, 714
BpuEI CTTGAG 1 cut(s) 1185
BpuMI CCSGG 1 cut(s) 86
BsaI GGTCTC 2 cut(s) 990, 1017
BsaJI CCNNGG 8 cut(s) 71, 72, 85, 91, 465, 658, 738, 785
BsaWI WCCGGW 1 cut(s) 567
BsaXI ACNNNNNCTCC 2 cut(s) 315, 345
Bsc4I CCNNNNNNNGG 4 cut(s) 379, 522, 574, 847
Bse1I ACTGG 4 cut(s) 187, 697, 1010, 1040
Bse3DI GCAATG 3 cut(s) 132, 486, 636
BseAI TCCGGA 1 cut(s) 567
BseBI CCWGG 9 cut(s) 73, 135, 674, 684, 786, 807, 937, 1275, 1310
BseDI CCNNGG 8 cut(s) 71, 72, 85, 91, 465, 658, 738, 785
BseGI GGATG 3 cut(s) 178, 493, 1262
BseLI CCNNNNNNNGG 4 cut(s) 379, 522, 574, 847
BseMI GCAATG 3 cut(s) 132, 486, 636
BseNI ACTGG 4 cut(s) 187, 697, 1010, 1040
BseSI GKGCMC 1 cut(s) 234
BseXI GCAGC 3 cut(s) 257, 940, 1259
BsgI GTGCAG 1 cut(s) 264
BshFI GGCC 6 cut(s) 90, 232, 515, 610, 810, 935
BsiSI CCGG 2 cut(s) 85, 568
BslI CCNNNNNNNGG 4 cut(s) 379, 522, 574, 847
BsmAI GTCTC 3 cut(s) 944, 990, 1017
BsmI GAATGC 1 cut(s) 508
BsnI GGCC 6 cut(s) 90, 232, 515, 610, 810, 935
Bso31I GGTCTC 2 cut(s) 990, 1017
Bsp120I GGGCCC 1 cut(s) 230
Bsp1286I GDGCHC 1 cut(s) 234
Bsp13I TCCGGA 1 cut(s) 567
Bsp143I GATC 3 cut(s) 812, 913, 1117
BspACI CCGC 4 cut(s) 497, 1091, 1244, 1278
BspANI GGCC 6 cut(s) 90, 232, 515, 610, 810, 935
BspEI TCCGGA 1 cut(s) 567
BspHI TCATGA 1 cut(s) 868
BspLI GGNNCC 6 cut(s) 6, 89, 232, 836, 941, 1185
BspMAI CTGCAG 1 cut(s) 762
BspMI ACCTGC 1 cut(s) 854
BspPI GGATC 1 cut(s) 1112
BspTNI GGTCTC 2 cut(s) 990, 1017
BsrDI GCAATG 3 cut(s) 132, 486, 636
BsrI ACTGG 4 cut(s) 187, 697, 1010, 1040
BssECI CCNNGG 8 cut(s) 71, 72, 85, 91, 465, 658, 738, 785
BssMI GATC 3 cut(s) 812, 913, 1117
BssT1I CCWWGG 3 cut(s) 465, 658, 738
Bst2UI CCWGG 9 cut(s) 73, 135, 674, 684, 786, 807, 937, 1275, 1310
Bst4CI ACNGT 5 cut(s) 240, 389, 1099, 1138, 1214
Bst6I CTCTTC 1 cut(s) 1134
BstC8I GCNNGC 2 cut(s) 402, 933
BstF5I GGATG 3 cut(s) 178, 493, 1262
BstKTI GATC 3 cut(s) 815, 916, 1120
BstMAI GTCTC 3 cut(s) 944, 990, 1017
BstMBI GATC 3 cut(s) 812, 913, 1117
BstMWI GCNNNNNNNGC 6 cut(s) 122, 512, 686, 749, 937, 1088
BstNI CCWGG 9 cut(s) 73, 135, 674, 684, 786, 807, 937, 1275, 1310
BstNSI RCATGY 1 cut(s) 1169
BstSFI CTRYAG 5 cut(s) 758, 852, 909, 921, 1083
BstSLI GKGCMC 1 cut(s) 234
BstV1I GCAGC 3 cut(s) 257, 940, 1259
BsuI GTATCC 1 cut(s) 575
BsuRI GGCC 6 cut(s) 90, 232, 515, 610, 810, 935
BtsCI GGATG 3 cut(s) 178, 493, 1262
BtsI GCAGTG 1 cut(s) 755
BtsIMutI CAGTG 2 cut(s) 755, 1219
BveI ACCTGC 1 cut(s) 854
Cac8I GCNNGC 2 cut(s) 402, 933
CciI TCATGA 1 cut(s) 868
Cfr13I GGNCC 7 cut(s) 4, 88, 230, 231, 338, 513, 544
CsiI ACCWGGT 1 cut(s) 133
Csp6I GTAC 2 cut(s) 106, 976
CviAII CATG 4 cut(s) 109, 599, 869, 1166
CviQI GTAC 2 cut(s) 106, 976
DpnI GATC 3 cut(s) 814, 915, 1119
DpnII GATC 3 cut(s) 812, 913, 1117
DraIII CACNNNGTG 1 cut(s) 137
EaeI YGGCCR 1 cut(s) 933
Eam1104I CTCTTC 1 cut(s) 1134
EarI CTCTTC 1 cut(s) 1134
Eco130I CCWWGG 3 cut(s) 465, 658, 738
Eco24I GRGCYC 1 cut(s) 234
Eco31I GGTCTC 2 cut(s) 990, 1017
Eco32I GATATC 1 cut(s) 670
Eco47I GGWCC 3 cut(s) 4, 338, 544
Eco57I CTGAAG 2 cut(s) 426, 540
EcoO109I RGGNCCY 2 cut(s) 230, 338
EcoRII CCWGG 9 cut(s) 71, 133, 672, 682, 784, 805, 935, 1273, 1308
EcoRV GATATC 1 cut(s) 670
EcoT14I CCWWGG 3 cut(s) 465, 658, 738
EcoT38I GRGCYC 1 cut(s) 234
ErhI CCWWGG 3 cut(s) 465, 658, 738
FaeI CATG 4 cut(s) 112, 602, 872, 1169
FatI CATG 4 cut(s) 108, 598, 868, 1165
Fnu4HI GCNGC 4 cut(s) 246, 929, 1245, 1248
FokI GGATG 3 cut(s) 185, 480, 1269
FriOI GRGCYC 1 cut(s) 234
Fsp4HI GCNGC 4 cut(s) 246, 929, 1245, 1248
FspBI CTAG 2 cut(s) 413, 1316
GluI GCNGC 4 cut(s) 246, 929, 1245, 1248
GsuI CTGGAG 2 cut(s) 705, 714
HaeIII GGCC 6 cut(s) 90, 232, 515, 610, 810, 935
HapII CCGG 2 cut(s) 85, 568
Hin1II CATG 4 cut(s) 112, 602, 872, 1169
HincII GTYRAC 1 cut(s) 454
HindII GTYRAC 1 cut(s) 454
HindIII AAGCTT 2 cut(s) 1203, 1320
HinfI GANTC 4 cut(s) 294, 481, 956, 1217
HpaI GTTAAC 1 cut(s) 454
HpaII CCGG 2 cut(s) 85, 568
HphI GGTGA 5 cut(s) 38, 123, 149, 1160, 1292
Hpy166II GTNNAC 2 cut(s) 392, 454
Hpy188I TCNGA 3 cut(s) 293, 520, 817
Hpy188III TCNNGA 6 cut(s) 278, 363, 413, 568, 869, 1115
Hpy8I GTNNAC 2 cut(s) 392, 454
HpyAV CCTTC 3 cut(s) 221, 373, 515
HpyCH4III ACNGT 5 cut(s) 240, 389, 1099, 1138, 1214
HpyCH4IV ACGT 1 cut(s) 1267
HpyF10VI GCNNNNNNNGC 6 cut(s) 122, 512, 686, 749, 937, 1088
HpySE526I ACGT 1 cut(s) 1267
Hsp92II CATG 4 cut(s) 112, 602, 872, 1169
Kpn2I TCCGGA 1 cut(s) 567
KspAI GTTAAC 1 cut(s) 454
Kzo9I GATC 3 cut(s) 812, 913, 1117
LmnI GCTCC 8 cut(s) 113, 628, 685, 686, 757, 945, 1183, 1312
Lsp1109I GCAGC 3 cut(s) 257, 940, 1259
LweI GCATC 1 cut(s) 850
MabI ACCWGGT 1 cut(s) 133
MaeI CTAG 2 cut(s) 413, 1316
MaeII ACGT 1 cut(s) 1267
MaeIII GTNAC 4 cut(s) 35, 62, 129, 988
MalI GATC 3 cut(s) 814, 915, 1119
MboI GATC 3 cut(s) 812, 913, 1117
MboII GAAGA 5 cut(s) 233, 236, 538, 803, 1121
MhlI GDGCHC 1 cut(s) 234
MlsI TGGCCA 1 cut(s) 935
MluCI AATT 5 cut(s) 157, 186, 343, 968, 1303
MluNI TGGCCA 1 cut(s) 935
MlyI GAGTC 1 cut(s) 303
MmeI TCCRAC 3 cut(s) 650, 1157, 1181
Mox20I TGGCCA 1 cut(s) 935
MroI TCCGGA 1 cut(s) 567
MscI TGGCCA 1 cut(s) 935
MseI TTAA 4 cut(s) 150, 347, 453, 1233
MslI CAYNNNNRTG 1 cut(s) 318
Msp20I TGGCCA 1 cut(s) 935
MspA1I CMGCKG 2 cut(s) 499, 931
MspI CCGG 2 cut(s) 85, 568
Mva1269I GAATGC 1 cut(s) 508
MvaI CCWGG 9 cut(s) 73, 135, 674, 684, 786, 807, 937, 1275, 1310
MwoI GCNNNNNNNGC 6 cut(s) 122, 512, 686, 749, 937, 1088
NciI CCSGG 1 cut(s) 86
NdeII GATC 3 cut(s) 812, 913, 1117
NlaIII CATG 4 cut(s) 112, 602, 872, 1169
NlaIV GGNNCC 6 cut(s) 6, 89, 232, 836, 941, 1185
NmeAIII GCCGAG 1 cut(s) 116
NmuCI GTSAC 2 cut(s) 35, 129
NspI RCATGY 1 cut(s) 1169
PagI TCATGA 1 cut(s) 868
PasI CCCWGGG 1 cut(s) 72
PctI GAATGC 1 cut(s) 508
PfeI GAWTC 3 cut(s) 481, 956, 1217
PfoI TCCNGGA 3 cut(s) 672, 682, 1308
PkrI GCNGC 4 cut(s) 247, 930, 1246, 1249
PleI GAGTC 1 cut(s) 302
PpsI GAGTC 1 cut(s) 302
PpuMI RGGWCCY 1 cut(s) 338
Psp5II RGGWCCY 1 cut(s) 338
Psp6I CCWGG 9 cut(s) 71, 133, 672, 682, 784, 805, 935, 1273, 1308
PspGI CCWGG 9 cut(s) 71, 133, 672, 682, 784, 805, 935, 1273, 1308
PspN4I GGNNCC 6 cut(s) 6, 89, 232, 836, 941, 1185
PspOMI GGGCCC 1 cut(s) 230
PspPI GGNCC 7 cut(s) 4, 88, 230, 231, 338, 513, 544
PspPPI RGGWCCY 1 cut(s) 338
PstI CTGCAG 1 cut(s) 762
PvuII CAGCTG 1 cut(s) 931
RsaI GTAC 2 cut(s) 107, 977
RsaNI GTAC 2 cut(s) 106, 976
RseI CAYNNNNRTG 1 cut(s) 318
SaqAI TTAA 4 cut(s) 150, 347, 453, 1233
SatI GCNGC 4 cut(s) 246, 929, 1245, 1248
Sau3AI GATC 3 cut(s) 812, 913, 1117
Sau96I GGNCC 7 cut(s) 4, 88, 230, 231, 338, 513, 544
ScaI AGTACT 1 cut(s) 977
SchI GAGTC 1 cut(s) 303
SduI GDGCHC 1 cut(s) 234
SexAI ACCWGGT 1 cut(s) 133
SfaNI GCATC 1 cut(s) 850
SfcI CTRYAG 5 cut(s) 758, 852, 909, 921, 1083
SinI GGWCC 3 cut(s) 4, 338, 544
SmiMI CAYNNNNRTG 1 cut(s) 318
SmlI CTYRAG 1 cut(s) 1200
SmoI CTYRAG 1 cut(s) 1200
Sse9I AATT 5 cut(s) 157, 186, 343, 968, 1303
SsiI CCGC 4 cut(s) 497, 1091, 1244, 1278
SspMI CTAG 2 cut(s) 413, 1316
StyI CCWWGG 3 cut(s) 465, 658, 738
TaaI ACNGT 5 cut(s) 240, 389, 1099, 1138, 1214
TaiI ACGT 1 cut(s) 1270
TaqI TCGA 3 cut(s) 252, 916, 1209
TasI AATT 5 cut(s) 157, 186, 343, 968, 1303
TatI WGTACW 2 cut(s) 105, 975
TauI GCSGC 1 cut(s) 1247
TfiI GAWTC 3 cut(s) 481, 956, 1217
Tru1I TTAA 4 cut(s) 150, 347, 453, 1233
Tru9I TTAA 4 cut(s) 150, 347, 453, 1233
TscAI CASTG 2 cut(s) 762, 1219
TseFI GTSAC 2 cut(s) 35, 129
TseI GCWGC 3 cut(s) 245, 928, 1247
Tsp45I GTSAC 2 cut(s) 35, 129
TspDTI ATGAA 4 cut(s) 189, 300, 810, 1050
TspRI CASTG 2 cut(s) 762, 1219
VpaK11BI GGWCC 3 cut(s) 4, 338, 544
XapI RAATTY 2 cut(s) 968, 1303
XbaI TCTAGA 1 cut(s) 412
XceI RCATGY 1 cut(s) 1169
XspI CTAG 2 cut(s) 413, 1316
ZrmI AGTACT 1 cut(s) 977
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.