Rorug05G0268300

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001972
Physical Location & Seq
Forward (+)
30310009 .. 30313625
3617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0268300.1

Sequence Viewer

Length: 1002 bp
ATGGTTCATGCTCTTTTACAAACAGCACCACTTCTCCTCCAGTGTTCTCCTCATGTCTCTTCCTGTAGGGGAAAGCCTGGGAAAATAGTGATGAAGTGCTGTGCAGAGCAATCTATGAGCAACAAGCCAGTGACCAAACATGTATTATCTGGGCTTGCGGCATCTTTAGTACTTCTCTCTCAAATAAACCAGGCTGTTGCATCAGAAGTTTACTTTCAAAGAAATACGTATGAGCTTGCAAATGCTGCTGAGAAGACAGTGACTCTTCCTCTTGATAAGGGTTCTGATGAAGGAGCTGAGAGGCTAATGATGATGAGAGGCATGACAGCAAACAATTTTGACCCCATTAGATATTCTGGTAGGTGGTATGAAGTTGCTTCACTTAAACGTGGATTTGCTGGGCAAGGTCAGGAAGACTGTCATTGCACCCAGGGTGTATATACCTTTGATAGTGAGAAGAGGGCCATCCAGGTCGATACCTTCTGTGTTCATGGGTCTCCTGACGGTTATATAACTGGCATAAGGGGAAACGTTCAATGCCTTTCAGAGAAAGATTTGGAAAAGAATGAGTCAGATCTAGAAATGCAGGAGATGATCAAAGAGAAGTGTTTCCTCCGTTTTCCAACATTGCCATTTATCCCTAAGTTGCCGTATGATGTGATCGCAACTGATTATGACAATTTTGCTCTTGTTTCAGGAGCAAAAGATACAGGTTTCATACAGATCTACTCCAGGACACCAACTCCTGGTCCTGAATTCATAGAGAAGTACAAATCATACTTGGCCAACTTTGGATATGACCCAACCAAAATCAAGGACACCCCACAAGACTGTGAAGTGATGTCGGACAGTCGGCTATCTGCAATGATGTCAATGTCCGGAATGCAAAAAATTTTAACAAATGAGTTTCCTGATCTAGAATTGAAGCGTCCTGTTCAATTCGACCCCTTCACTAGTGTATTTGACACTCTGAAGAAGCTTGTTCAGCTTTATTTCAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metalloenzyme PhosphMutase

Protein Analysis

333

Amino Acids

37.37

Weight (kDa)

6.02

Isoelectric Point (pI)

35.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Lipocalin_2 PF08212 116 - 276 5.3e-10 Lipocalin-like domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 746
AccIII TCCGGA 1 cut(s) 878
AciI CCGC 1 cut(s) 158
AclI AACGTT 1 cut(s) 531
AcoI YGGCCR 1 cut(s) 783
AcsI RAATTY 2 cut(s) 755, 891
AcuI CTGAAG 1 cut(s) 992
AfaI GTAC 2 cut(s) 171, 770
AfiI CCNNNNNNNGG 1 cut(s) 746
AflIII ACRYGT 1 cut(s) 139
AgsI TTSAA 5 cut(s) 218, 536, 925, 938, 997
AhlI ACTAGT 1 cut(s) 953
AjnI CCWGG 6 cut(s) 76, 189, 429, 468, 731, 745
AluBI AGCT 4 cut(s) 235, 296, 979, 988
AluI AGCT 4 cut(s) 235, 296, 979, 988
Alw26I GTCTC 2 cut(s) 61, 501
Aor13HI TCCGGA 1 cut(s) 878
AoxI GGCC 2 cut(s) 462, 783
ApeKI GCWGC 1 cut(s) 245
ApoI RAATTY 2 cut(s) 755, 891
Asp700I GAANNNNTTC 1 cut(s) 608
AspS9I GGNCC 2 cut(s) 462, 749
AvaII GGWCC 1 cut(s) 749
BalI TGGCCA 1 cut(s) 785
BbsI GAAGAC 2 cut(s) 260, 420
BbvI GCAGC 1 cut(s) 232
BccI CCATC 1 cut(s) 473
BceAI ACGGC 1 cut(s) 634
BciT130I CCWGG 6 cut(s) 78, 191, 431, 470, 733, 747
BclI TGATCA 1 cut(s) 594
BcoDI GTCTC 2 cut(s) 61, 501
BcuI ACTAGT 1 cut(s) 953
BfaI CTAG 3 cut(s) 578, 917, 954
BfmI CTRYAG 1 cut(s) 64
BglII AGATCT 2 cut(s) 574, 723
BisI GCNGC 2 cut(s) 159, 246
BlsI GCNGC 2 cut(s) 160, 247
BmcAI AGTACT 1 cut(s) 171
Bme1390I CCNGG 6 cut(s) 78, 191, 431, 470, 733, 747
Bme18I GGWCC 1 cut(s) 749
BmgT120I GGNCC 2 cut(s) 462, 749
BmrFI CCNGG 6 cut(s) 78, 191, 431, 470, 733, 747
BmsI GCATC 2 cut(s) 170, 209
BpiI GAAGAC 2 cut(s) 260, 420
BpmI CTGGAG 2 cut(s) 23, 715
BsaAI YACGTR 1 cut(s) 228
BsaI GGTCTC 1 cut(s) 501
BsaJI CCNNGG 3 cut(s) 77, 429, 430
BsaWI WCCGGW 1 cut(s) 878
BsaXI ACNNNNNCTCC 6 cut(s) 18, 21, 48, 51, 727, 757
Bsc4I CCNNNNNNNGG 1 cut(s) 746
Bse1I ACTGG 3 cut(s) 40, 128, 520
Bse3DI GCAATG 3 cut(s) 421, 626, 870
BseAI TCCGGA 1 cut(s) 878
BseBI CCWGG 6 cut(s) 78, 191, 431, 470, 733, 747
BseDI CCNNGG 3 cut(s) 77, 429, 430
BseGI GGATG 1 cut(s) 465
BseLI CCNNNNNNNGG 1 cut(s) 746
BseMI GCAATG 3 cut(s) 421, 626, 870
BseMII CTCAG 2 cut(s) 240, 288
BseNI ACTGG 3 cut(s) 40, 128, 520
BseRI GAGGAG 2 cut(s) 26, 39
BseXI GCAGC 1 cut(s) 232
BseYI CCCAGC 1 cut(s) 398
BsgI GTGCAG 1 cut(s) 123
BshFI GGCC 2 cut(s) 464, 785
BsiSI CCGG 1 cut(s) 879
BslI CCNNNNNNNGG 1 cut(s) 746
BsmAI GTCTC 2 cut(s) 61, 501
BsmI GAATGC 1 cut(s) 888
BsnI GGCC 2 cut(s) 464, 785
Bso31I GGTCTC 1 cut(s) 501
Bsp13I TCCGGA 1 cut(s) 878
Bsp143I GATC 5 cut(s) 574, 594, 660, 723, 913
BspACI CCGC 1 cut(s) 158
BspANI GGCC 2 cut(s) 464, 785
BspCNI CTCAG 2 cut(s) 241, 289
BspEI TCCGGA 1 cut(s) 878
BspTNI GGTCTC 1 cut(s) 501
BsrDI GCAATG 3 cut(s) 421, 626, 870
BsrI ACTGG 3 cut(s) 40, 128, 520
BssECI CCNNGG 3 cut(s) 77, 429, 430
BssMI GATC 5 cut(s) 574, 594, 660, 723, 913
Bst2UI CCWGG 6 cut(s) 78, 191, 431, 470, 733, 747
Bst4CI ACNGT 5 cut(s) 259, 419, 506, 833, 851
Bst6I CTCTTC 3 cut(s) 64, 270, 452
BstAPI GCANNNNNTGC 1 cut(s) 245
BstBAI YACGTR 1 cut(s) 228
BstC8I GCNNGC 2 cut(s) 156, 237
BstDEI CTNAG 3 cut(s) 249, 297, 642
BstF5I GGATG 1 cut(s) 465
BstKTI GATC 5 cut(s) 577, 597, 663, 726, 916
BstMAI GTCTC 2 cut(s) 61, 501
BstMBI GATC 5 cut(s) 574, 594, 660, 723, 913
BstMWI GCNNNNNNNGC 2 cut(s) 245, 985
BstNI CCWGG 6 cut(s) 78, 191, 431, 470, 733, 747
BstNSI RCATGY 1 cut(s) 143
BstSCI CCNGG 6 cut(s) 76, 189, 429, 468, 731, 745
BstSFI CTRYAG 1 cut(s) 64
BstSNI TACGTA 1 cut(s) 228
BstV1I GCAGC 1 cut(s) 232
BstV2I GAAGAC 2 cut(s) 260, 420
BstX2I RGATCY 2 cut(s) 574, 723
BstYI RGATCY 2 cut(s) 574, 723
BsuRI GGCC 2 cut(s) 464, 785
BtsCI GGATG 1 cut(s) 465
BtsIMutI CAGTG 3 cut(s) 47, 135, 264
Cac8I GCNNGC 2 cut(s) 156, 237
Cfr13I GGNCC 2 cut(s) 462, 749
CseI GACGC 1 cut(s) 917
Csp6I GTAC 2 cut(s) 170, 769
CviAII CATG 5 cut(s) 8, 53, 140, 322, 491
CviQI GTAC 2 cut(s) 170, 769
DdeI CTNAG 3 cut(s) 249, 297, 642
DpnI GATC 5 cut(s) 576, 596, 662, 725, 915
DpnII GATC 5 cut(s) 574, 594, 660, 723, 913
EaeI YGGCCR 1 cut(s) 783
Eam1104I CTCTTC 3 cut(s) 64, 270, 452
EarI CTCTTC 3 cut(s) 64, 270, 452
Eco105I TACGTA 1 cut(s) 228
Eco31I GGTCTC 1 cut(s) 501
Eco47I GGWCC 1 cut(s) 749
Eco57I CTGAAG 1 cut(s) 992
EcoRI GAATTC 1 cut(s) 755
EcoRII CCWGG 6 cut(s) 76, 189, 429, 468, 731, 745
FaeI CATG 5 cut(s) 11, 56, 143, 325, 494
FatI CATG 5 cut(s) 7, 52, 139, 321, 490
FbaI TGATCA 1 cut(s) 594
Fnu4HI GCNGC 2 cut(s) 159, 246
FokI GGATG 1 cut(s) 452
Fsp4HI GCNGC 2 cut(s) 159, 246
FspBI CTAG 3 cut(s) 578, 917, 954
GluI GCNGC 2 cut(s) 159, 246
GsaI CCCAGC 1 cut(s) 402
GsuI CTGGAG 2 cut(s) 23, 715
HaeIII GGCC 2 cut(s) 464, 785
HapII CCGG 1 cut(s) 879
HgaI GACGC 1 cut(s) 917
Hin1II CATG 5 cut(s) 11, 56, 143, 325, 494
HindIII AAGCTT 1 cut(s) 977
HinfI GANTC 2 cut(s) 262, 569
HpaII CCGG 1 cut(s) 879
Hpy166II GTNNAC 1 cut(s) 211
Hpy188I TCNGA 6 cut(s) 205, 286, 547, 574, 847, 972
Hpy188III TCNNGA 9 cut(s) 272, 410, 500, 578, 696, 752, 879, 911, 917
Hpy8I GTNNAC 1 cut(s) 211
HpyAV CCTTC 3 cut(s) 284, 490, 958
HpyCH4III ACNGT 5 cut(s) 259, 419, 506, 833, 851
HpyCH4IV ACGT 3 cut(s) 227, 388, 531
HpyCH4V TGCA 7 cut(s) 104, 200, 239, 426, 586, 863, 886
HpyF10VI GCNNNNNNNGC 2 cut(s) 245, 985
HpyF3I CTNAG 3 cut(s) 249, 297, 642
HpySE526I ACGT 3 cut(s) 227, 388, 531
Hsp92II CATG 5 cut(s) 11, 56, 143, 325, 494
Kpn2I TCCGGA 1 cut(s) 878
Ksp22I TGATCA 1 cut(s) 594
Kzo9I GATC 5 cut(s) 574, 594, 660, 723, 913
LmnI GCTCC 2 cut(s) 293, 698
Lsp1109I GCAGC 1 cut(s) 232
LweI GCATC 2 cut(s) 170, 209
MaeI CTAG 3 cut(s) 578, 917, 954
MaeII ACGT 3 cut(s) 227, 388, 531
MaeIII GTNAC 2 cut(s) 130, 259
MalI GATC 5 cut(s) 576, 596, 662, 725, 915
MboI GATC 5 cut(s) 574, 594, 660, 723, 913
MboII GAAGA 6 cut(s) 51, 257, 265, 425, 469, 985
MflI RGATCY 2 cut(s) 574, 723
MlsI TGGCCA 1 cut(s) 785
MluCI AATT 6 cut(s) 334, 679, 755, 891, 920, 938
MluNI TGGCCA 1 cut(s) 785
MlyI GAGTC 2 cut(s) 256, 578
MmeI TCCRAC 2 cut(s) 647, 825
MnlI CCTC 7 cut(s) 47, 60, 279, 294, 311, 453, 623
Mox20I TGGCCA 1 cut(s) 785
MroI TCCGGA 1 cut(s) 878
MroXI GAANNNNTTC 1 cut(s) 608
MscI TGGCCA 1 cut(s) 785
MseI TTAA 2 cut(s) 384, 896
Msp20I TGGCCA 1 cut(s) 785
MspI CCGG 1 cut(s) 879
MspR9I CCNGG 6 cut(s) 78, 191, 431, 470, 733, 747
Mva1269I GAATGC 1 cut(s) 888
MvaI CCWGG 6 cut(s) 78, 191, 431, 470, 733, 747
MwoI GCNNNNNNNGC 2 cut(s) 245, 985
NdeII GATC 5 cut(s) 574, 594, 660, 723, 913
NlaIII CATG 5 cut(s) 11, 56, 143, 325, 494
NmuCI GTSAC 2 cut(s) 130, 259
NspI RCATGY 1 cut(s) 143
PasI CCCWGGG 1 cut(s) 430
PciI ACATGT 1 cut(s) 139
PctI GAATGC 1 cut(s) 888
PdmI GAANNNNTTC 1 cut(s) 608
PflMI CCANNNNNTGG 1 cut(s) 746
PfoI TCCNGGA 1 cut(s) 731
PkrI GCNGC 2 cut(s) 160, 247
PleI GAGTC 2 cut(s) 256, 577
PpsI GAGTC 2 cut(s) 256, 577
Ppu21I YACGTR 1 cut(s) 228
PscI ACATGT 1 cut(s) 139
Psp1406I AACGTT 1 cut(s) 531
Psp6I CCWGG 6 cut(s) 76, 189, 429, 468, 731, 745
PspFI CCCAGC 1 cut(s) 398
PspGI CCWGG 6 cut(s) 76, 189, 429, 468, 731, 745
PspPI GGNCC 2 cut(s) 462, 749
PsuI RGATCY 2 cut(s) 574, 723
RsaI GTAC 2 cut(s) 171, 770
RsaNI GTAC 2 cut(s) 170, 769
SaqAI TTAA 2 cut(s) 384, 896
SatI GCNGC 2 cut(s) 159, 246
Sau3AI GATC 5 cut(s) 574, 594, 660, 723, 913
Sau96I GGNCC 2 cut(s) 462, 749
ScaI AGTACT 1 cut(s) 171
SchI GAGTC 2 cut(s) 256, 578
ScrFI CCNGG 6 cut(s) 78, 191, 431, 470, 733, 747
SfaNI GCATC 2 cut(s) 170, 209
SfcI CTRYAG 1 cut(s) 64
SinI GGWCC 1 cut(s) 749
SnaBI TACGTA 1 cut(s) 228
SpeI ACTAGT 1 cut(s) 953
Sse9I AATT 6 cut(s) 334, 679, 755, 891, 920, 938
SsiI CCGC 1 cut(s) 158
SspMI CTAG 3 cut(s) 578, 917, 954
StyD4I CCNGG 6 cut(s) 76, 189, 429, 468, 731, 745
TaaI ACNGT 5 cut(s) 259, 419, 506, 833, 851
TaiI ACGT 3 cut(s) 230, 391, 534
TaqI TCGA 2 cut(s) 474, 942
TasI AATT 6 cut(s) 334, 679, 755, 891, 920, 938
TatI WGTACW 2 cut(s) 169, 768
TauI GCSGC 1 cut(s) 161
Tru1I TTAA 2 cut(s) 384, 896
Tru9I TTAA 2 cut(s) 384, 896
TscAI CASTG 3 cut(s) 47, 135, 264
TseFI GTSAC 2 cut(s) 130, 259
TseI GCWGC 1 cut(s) 245
Tsp45I GTSAC 2 cut(s) 130, 259
TspDTI ATGAA 6 cut(s) 107, 303, 384, 479, 706, 748
TspGWI ACGGA 1 cut(s) 605
TspRI CASTG 3 cut(s) 47, 135, 264
Van91I CCANNNNNTGG 1 cut(s) 746
VpaK11BI GGWCC 1 cut(s) 749
XapI RAATTY 2 cut(s) 755, 891
XbaI TCTAGA 2 cut(s) 577, 916
XceI RCATGY 1 cut(s) 143
XmnI GAANNNNTTC 1 cut(s) 608
XspI CTAG 3 cut(s) 578, 917, 954
ZrmI AGTACT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.