Rh5DG231500

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0001972
Physical Location & Seq
Forward (+)
26410958 .. 26413463
2506 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG231500.1

Sequence Viewer

Length: 414 bp
ATGGCTCTTCATTGCCTATCTTCTTCCTGCTTAGCCCTTCCTTCCAAATTCGGCAACCTTTCCCTCAATGGCCCTTCTACTTCTTCAGCTTCCACCCATTTCCCTTCCCTCTCTTTCTCCGCTAACCTCTCTCACGATTTCTTCTCCCAAGGGTATTTGTGTGTGAGCCCAAGTCAGAGGTCAGTTCGTCGCTCTGTGGTTTGTGAGGCAGCTCCAACCAAGAAGCCTGATTCTGCTGAAAAGAGAGCTCGCCAGGCTGAGAAAAGGCGCATTTACAACAAGTCCAGAAAGTCTGAAATCAAAACCAGGATGAAGAAGGTCATTTCTTATCATATTTACATTCTCTGTGAAGTAAATTTATTCGGATTTTTTTTTTTTTTTTTTTTTTTTTGTGAATATGTGAAGATGCTATGA

Protein Analysis

137

Amino Acids

15.62

Weight (kDa)

9.76

Isoelectric Point (pI)

65.54

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 77 - 108 1.2e-07 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 120
AcsI RAATTY 2 cut(s) 47, 355
AcuI CTGAAG 1 cut(s) 69
AjnI CCWGG 2 cut(s) 252, 305
AluBI AGCT 3 cut(s) 89, 212, 248
AluI AGCT 3 cut(s) 89, 212, 248
Alw21I GWGCWC 1 cut(s) 250
AoxI GGCC 1 cut(s) 70
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 2 cut(s) 47, 355
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 71
BanII GRGCYC 2 cut(s) 170, 250
Bbv12I GWGCWC 1 cut(s) 250
BbvI GCAGC 1 cut(s) 221
BciT130I CCWGG 2 cut(s) 254, 307
BisI GCNGC 1 cut(s) 210
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 2 cut(s) 254, 307
BmgT120I GGNCC 1 cut(s) 71
BmrFI CCNGG 2 cut(s) 254, 307
BmsI GCATC 1 cut(s) 396
Bpu1102I GCTNAGC 1 cut(s) 31
BsaJI CCNNGG 1 cut(s) 148
Bse3DI GCAATG 1 cut(s) 10
BseBI CCWGG 2 cut(s) 254, 307
BseDI CCNNGG 1 cut(s) 148
BseGI GGATG 1 cut(s) 315
BseMI GCAATG 1 cut(s) 10
BseMII CTCAG 1 cut(s) 249
BseXI GCAGC 1 cut(s) 221
BshFI GGCC 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 250
BsnI GGCC 1 cut(s) 72
Bsp1286I GDGCHC 2 cut(s) 170, 250
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 1 cut(s) 120
BspANI GGCC 1 cut(s) 72
BspCNI CTCAG 1 cut(s) 250
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 1 cut(s) 10
BssECI CCNNGG 1 cut(s) 148
BssT1I CCWWGG 1 cut(s) 148
Bst2UI CCWGG 2 cut(s) 254, 307
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 1 cut(s) 250
BstDEI CTNAG 2 cut(s) 31, 258
BstF5I GGATG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 270
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 254, 307
BstSCI CCNGG 2 cut(s) 252, 305
BstV1I GCAGC 1 cut(s) 221
BsuRI GGCC 1 cut(s) 72
BtsCI GGATG 1 cut(s) 315
Cac8I GCNNGC 1 cut(s) 250
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 71
CviJI RGCY 9 cut(s) 5, 35, 72, 89, 168, 212, 226, 248, 257
CviKI_1 RGCY 9 cut(s) 5, 35, 72, 89, 168, 212, 226, 248, 257
DdeI CTNAG 2 cut(s) 31, 258
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 2 cut(s) 170, 250
Eco53kI GAGCTC 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 69
EcoICRI GAGCTC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 252, 305
EcoT14I CCWWGG 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 170, 250
ErhI CCWWGG 1 cut(s) 148
FaiI YATR 3 cut(s) 333, 399, 412
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 2 cut(s) 170, 250
Fsp4HI GCNGC 1 cut(s) 210
GlaI GCGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 210
HaeIII GGCC 1 cut(s) 72
HhaI GCGC 1 cut(s) 270
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HinfI GANTC 1 cut(s) 230
Hpy188I TCNGA 3 cut(s) 177, 295, 365
Hpy188III TCNNGA 2 cut(s) 134, 285
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 5 cut(s) 47, 51, 84, 114, 310
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 2 cut(s) 31, 258
HspAI GCGC 1 cut(s) 268
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 1 cut(s) 217
LpnPI CCDG 7 cut(s) 40, 239, 240, 266, 292, 298, 319
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 396
MboII GAAGA 5 cut(s) 12, 15, 75, 133, 325
MhlI GDGCHC 2 cut(s) 170, 250
MluCI AATT 2 cut(s) 47, 355
MmeI TCCRAC 1 cut(s) 239
MnlI CCTC 5 cut(s) 74, 119, 137, 171, 199
MspR9I CCNGG 2 cut(s) 254, 307
MvaI CCWGG 2 cut(s) 254, 307
MwoI GCNNNNNNNGC 1 cut(s) 254
PciSI GCTCTTC 1 cut(s) 12
PfeI GAWTC 1 cut(s) 230
PkrI GCNGC 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 252, 305
PspGI CCWGG 2 cut(s) 252, 305
PspPI GGNCC 1 cut(s) 71
SacI GAGCTC 1 cut(s) 250
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 1 cut(s) 210
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 2 cut(s) 254, 307
SduI GDGCHC 2 cut(s) 170, 250
SetI ASST 7 cut(s) 60, 91, 129, 182, 214, 250, 321
SfaNI GCATC 1 cut(s) 396
Sse9I AATT 2 cut(s) 47, 355
SsiI CCGC 1 cut(s) 120
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 2 cut(s) 252, 305
StyI CCWWGG 1 cut(s) 148
TasI AATT 2 cut(s) 47, 355
TfiI GAWTC 1 cut(s) 230
TseI GCWGC 1 cut(s) 209
TspDTI ATGAA 1 cut(s) 326
XapI RAATTY 2 cut(s) 47, 355
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.