MD11G1253300.v1.1

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0001972
Physical Location & Seq
Reverse (-)
36546181 .. 36548171
1991 bp
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UTR
Exon/CDS
Intron
MD11G1253300.v1.1.491

Sequence Viewer

Length: 555 bp
ATGTCAGCTGCAATTCATTGCCTATCTCCATGCCTATCCCTCCCCTCCAGATTCAGCAAGCTTTCACTCAATGGCCCTTCTACTTCCTCTGCCACTACTCATTCCACAACTTCCCTCAGTTTCTCAGCTACCCTTTCTCCCAATTTCTTCTCCAAAGGGTATCTGGCTGTGAGTCCACTTCAGAGGCCGGTTCGGCATTCCATTGTTTGTGAGGTCACCACGAAGAAGCCCGACTCTGCTGTGAAGAGAGCTCGACAGGCCGAAAAGAGGAGGATTTACAACAAATCCCGCAAATCCGAAATCAAAACCCGGATGAAGAAGGTTTTGGAAGCTCTGGATGGGCTGAAGAAGAAGCCAGATGCTGCACCCGAAGAGGTCCTTTCTATTGAGAAGCTCATTGGAGAGGCATACTCCATCATTGACAAAGCAGTTAAGGTGGGAACTCTGCACAGGAATACTGGAGCACGAAGAAAGTCTCGACTGGCTAGAAGAAAGAAAGCTGTTGAAATCCACCACGGCTGGTACACCCCAACTACAGAGGCTGCTAGTGCCTGA

Protein Analysis

185

Amino Acids

20.26

Weight (kDa)

10.37

Isoelectric Point (pI)

50.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 78 - 164 2e-22 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 289
AcuI CTGAAG 2 cut(s) 164, 365
AfaI GTAC 1 cut(s) 524
AfiI CCNNNNNNNGG 1 cut(s) 267
AgsI TTSAA 1 cut(s) 506
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 7 cut(s) 8, 61, 128, 251, 332, 394, 500
AluI AGCT 7 cut(s) 8, 61, 128, 251, 332, 394, 500
Alw21I GWGCWC 2 cut(s) 253, 466
Alw26I GTCTC 1 cut(s) 480
AlwNI CAGNNNCTG 2 cut(s) 362, 542
AoxI GGCC 3 cut(s) 73, 185, 258
ApeKI GCWGC 3 cut(s) 8, 362, 542
AspS9I GGNCC 2 cut(s) 74, 376
AsuC2I CCSGG 1 cut(s) 310
AsuHPI GGTGA 1 cut(s) 208
AvaII GGWCC 1 cut(s) 376
BanII GRGCYC 1 cut(s) 253
Bbv12I GWGCWC 2 cut(s) 253, 466
BbvI GCAGC 2 cut(s) 349, 529
BccI CCATC 2 cut(s) 332, 422
BceAI ACGGC 1 cut(s) 532
BcnI CCSGG 1 cut(s) 310
BcoDI GTCTC 1 cut(s) 480
BfaI CTAG 2 cut(s) 486, 546
BfmI CTRYAG 1 cut(s) 534
BglI GCCNNNNNGGC 1 cut(s) 193
BisI GCNGC 3 cut(s) 9, 363, 543
BlsI GCNGC 3 cut(s) 10, 364, 544
Bme1390I CCNGG 1 cut(s) 310
Bme18I GGWCC 1 cut(s) 376
BmgT120I GGNCC 2 cut(s) 74, 376
BmrFI CCNGG 1 cut(s) 310
BmsI GCATC 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpmI CTGGAG 2 cut(s) 31, 480
BpuMI CCSGG 1 cut(s) 310
BsaJI CCNNGG 1 cut(s) 514
BsaXI ACNNNNNCTCC 2 cut(s) 121, 151
Bsc4I CCNNNNNNNGG 1 cut(s) 267
Bse118I RCCGGY 1 cut(s) 187
Bse1I ACTGG 2 cut(s) 463, 486
Bse3DI GCAATG 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 514
BseGI GGATG 2 cut(s) 318, 343
BseLI CCNNNNNNNGG 1 cut(s) 267
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 130, 138
BseNI ACTGG 2 cut(s) 463, 486
BseRI GAGGAG 1 cut(s) 283
BseXI GCAGC 2 cut(s) 349, 529
BsgI GTGCAG 2 cut(s) 348, 431
BshFI GGCC 3 cut(s) 75, 187, 260
BsiHKAI GWGCWC 2 cut(s) 253, 466
BsiSI CCGG 2 cut(s) 188, 310
BslI CCNNNNNNNGG 1 cut(s) 267
BsmAI GTCTC 1 cut(s) 480
BsmI GAATGC 1 cut(s) 196
BsnI GGCC 3 cut(s) 75, 187, 260
Bsp1286I GDGCHC 2 cut(s) 253, 466
BspACI CCGC 1 cut(s) 289
BspANI GGCC 3 cut(s) 75, 187, 260
BspCNI CTCAG 2 cut(s) 129, 137
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 187
BsrI ACTGG 2 cut(s) 463, 486
BssAI RCCGGY 1 cut(s) 187
BssECI CCNNGG 1 cut(s) 514
Bst6I CTCTTC 2 cut(s) 239, 366
BstC8I GCNNGC 1 cut(s) 59
BstDEI CTNAG 2 cut(s) 116, 124
BstDSI CCRYGG 1 cut(s) 514
BstEII GGTNACC 1 cut(s) 214
BstF5I GGATG 2 cut(s) 318, 343
BstMAI GTCTC 1 cut(s) 480
BstMWI GCNNNNNNNGC 3 cut(s) 193, 257, 548
BstPI GGTNACC 1 cut(s) 214
BstSCI CCNGG 1 cut(s) 308
BstSFI CTRYAG 1 cut(s) 534
BstV1I GCAGC 2 cut(s) 349, 529
BsuRI GGCC 3 cut(s) 75, 187, 260
BtgI CCRYGG 1 cut(s) 514
BtsCI GGATG 2 cut(s) 318, 343
Cac8I GCNNGC 1 cut(s) 59
CaiI CAGNNNCTG 2 cut(s) 362, 542
Cfr10I RCCGGY 1 cut(s) 187
Cfr13I GGNCC 2 cut(s) 74, 376
Csp6I GTAC 1 cut(s) 523
CviAII CATG 1 cut(s) 30
CviQI GTAC 1 cut(s) 523
DdeI CTNAG 2 cut(s) 116, 124
Eam1104I CTCTTC 2 cut(s) 239, 366
EarI CTCTTC 2 cut(s) 239, 366
Ecl136II GAGCTC 1 cut(s) 251
Eco24I GRGCYC 1 cut(s) 253
Eco47I GGWCC 1 cut(s) 376
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 164, 365
Eco91I GGTNACC 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 251
EcoO109I RGGNCCY 1 cut(s) 376
EcoO65I GGTNACC 1 cut(s) 214
EcoT38I GRGCYC 1 cut(s) 253
FaeI CATG 1 cut(s) 33
FaiI YATR 2 cut(s) 31, 409
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FatI CATG 1 cut(s) 29
FauI CCCGC 1 cut(s) 296
Fnu4HI GCNGC 3 cut(s) 9, 363, 543
FokI GGATG 2 cut(s) 325, 350
FriOI GRGCYC 1 cut(s) 253
Fsp4HI GCNGC 3 cut(s) 9, 363, 543
FspBI CTAG 2 cut(s) 486, 546
GluI GCNGC 3 cut(s) 9, 363, 543
GsuI CTGGAG 2 cut(s) 31, 480
HaeIII GGCC 3 cut(s) 75, 187, 260
HapII CCGG 2 cut(s) 188, 310
Hin1II CATG 1 cut(s) 33
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 3 cut(s) 51, 172, 233
HpaII CCGG 2 cut(s) 188, 310
HphI GGTGA 1 cut(s) 208
Hpy166II GTNNAC 2 cut(s) 176, 525
Hpy188I TCNGA 2 cut(s) 183, 298
Hpy188III TCNNGA 3 cut(s) 48, 335, 477
Hpy8I GTNNAC 2 cut(s) 176, 525
HpyAV CCTTC 2 cut(s) 87, 313
HpyCH4V TGCA 3 cut(s) 11, 365, 448
HpyF10VI GCNNNNNNNGC 3 cut(s) 193, 257, 548
HpyF3I CTNAG 2 cut(s) 116, 124
Hsp92II CATG 1 cut(s) 33
LmnI GCTCC 1 cut(s) 461
Lsp1109I GCAGC 2 cut(s) 349, 529
LweI GCATC 1 cut(s) 349
MaeI CTAG 2 cut(s) 486, 546
MaeIII GTNAC 1 cut(s) 214
MboII GAAGA 9 cut(s) 139, 235, 256, 328, 358, 361, 383, 480, 501
MhlI GDGCHC 2 cut(s) 253, 466
MluCI AATT 2 cut(s) 12, 142
MlyI GAGTC 2 cut(s) 181, 227
MseI TTAA 1 cut(s) 432
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 188, 310
MspR9I CCNGG 1 cut(s) 310
Mva1269I GAATGC 1 cut(s) 196
MwoI GCNNNNNNNGC 3 cut(s) 193, 257, 548
NciI CCSGG 1 cut(s) 310
NlaIII CATG 1 cut(s) 33
NmuCI GTSAC 1 cut(s) 214
PctI GAATGC 1 cut(s) 196
PfeI GAWTC 1 cut(s) 51
PkrI GCNGC 3 cut(s) 10, 364, 544
PleI GAGTC 2 cut(s) 180, 227
PpsI GAGTC 2 cut(s) 180, 227
PpuMI RGGWCCY 1 cut(s) 376
Psp124BI GAGCTC 1 cut(s) 253
Psp5II RGGWCCY 1 cut(s) 376
PspEI GGTNACC 1 cut(s) 214
PspPI GGNCC 2 cut(s) 74, 376
PspPPI RGGWCCY 1 cut(s) 376
PstNI CAGNNNCTG 2 cut(s) 362, 542
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 1 cut(s) 524
RsaNI GTAC 1 cut(s) 523
SacI GAGCTC 1 cut(s) 253
SaqAI TTAA 1 cut(s) 432
SatI GCNGC 3 cut(s) 9, 363, 543
Sau96I GGNCC 2 cut(s) 74, 376
SchI GAGTC 2 cut(s) 181, 227
ScrFI CCNGG 1 cut(s) 310
SduI GDGCHC 2 cut(s) 253, 466
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 534
SinI GGWCC 1 cut(s) 376
Sse9I AATT 2 cut(s) 12, 142
SsiI CCGC 1 cut(s) 289
SspMI CTAG 2 cut(s) 486, 546
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 1 cut(s) 308
TaqI TCGA 2 cut(s) 253, 478
TasI AATT 2 cut(s) 12, 142
TfiI GAWTC 1 cut(s) 51
Tru1I TTAA 1 cut(s) 432
Tru9I TTAA 1 cut(s) 432
TseFI GTSAC 1 cut(s) 214
TseI GCWGC 3 cut(s) 8, 362, 542
Tsp45I GTSAC 1 cut(s) 214
TspDTI ATGAA 2 cut(s) 5, 329
VpaK11BI GGWCC 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 160
XspI CTAG 2 cut(s) 486, 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.