RchiOBHm_Chr5g0032711

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0001972
Physical Location & Seq
Forward (+)
26443821 .. 26446117
2297 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ31187

Sequence Viewer

Length: 552 bp
ATGGCTCTTCATTGCCTATCTTCTTCCTGCTTAGCCCTTCCTTCCAAATTCGGCAACCTTTCCCTCAATGGCCCTTGTACTTCCTCAGCTTCCACCCATTTCCCTTCCCTCTCTTTCTCCGCTAACCTCTCTCACGATTTCTTCTCCCAAGGGTATTTGTGTGTGAGCCCAAGTCAGAGGTCAGTTCGTCGCTCTGTGGTTTGTGAGGCAGCTCCAACCAAAAAGCCTGATTCTGCTGAAAAGAGAGCTCGCCAGGCTGAGAAAAGGCGCATTTACAACAAGTCCAGAAAGTCTGAAATCAAAACCAGGATGAAGAAGGTTTTGGAAGCTTTAGACGTGCTCAAGAAGAAACCAGATGCTCAGGCTGAAGAAGTTCTTTCAATTGAGAAGCTTATCGGCGAGGCATACTCCATCATTGACAAAGCAGTGAAGGTTGGAACATTGCATAGGAATACTGGAGCACGCAGAAAGTCTCGATTGGCCAGAAGAAAGAAGGCTGTAGAGATCCAAAAAGGCTGGTATACCCCGGCAGATTCTGAAGTCACTGCCTGA

Protein Analysis

183

Amino Acids

20.27

Weight (kDa)

10.02

Isoelectric Point (pI)

55.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 77 - 163 6.1e-22 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 521
AciI CCGC 1 cut(s) 120
AclWI GGATC 1 cut(s) 499
AcoI YGGCCR 1 cut(s) 480
AcsI RAATTY 1 cut(s) 47
AcuI CTGAAG 1 cut(s) 387
AfaI GTAC 1 cut(s) 79
AgsI TTSAA 1 cut(s) 381
AjiI CACGTC 1 cut(s) 337
AjnI CCWGG 2 cut(s) 252, 305
AjuI GAANNNNNNNTTGG 2 cut(s) 305, 337
AluBI AGCT 5 cut(s) 89, 212, 248, 329, 391
AluI AGCT 5 cut(s) 89, 212, 248, 329, 391
Alw21I GWGCWC 3 cut(s) 250, 342, 463
Alw26I GTCTC 1 cut(s) 477
AlwI GGATC 1 cut(s) 499
AlwNI CAGNNNCTG 1 cut(s) 536
AoxI GGCC 2 cut(s) 70, 480
ApeKI GCWGC 1 cut(s) 209
ApoI RAATTY 1 cut(s) 47
Asp700I GAANNNNTTC 1 cut(s) 372
AspLEI GCGC 1 cut(s) 270
AspS9I GGNCC 1 cut(s) 71
AsuC2I CCSGG 1 cut(s) 527
BalI TGGCCA 1 cut(s) 482
BanII GRGCYC 2 cut(s) 170, 250
Bbv12I GWGCWC 3 cut(s) 250, 342, 463
BbvCI CCTCAGC 1 cut(s) 85
BbvI GCAGC 1 cut(s) 221
BccI CCATC 1 cut(s) 419
BciT130I CCWGG 2 cut(s) 254, 307
BcnI CCSGG 1 cut(s) 527
BcoDI GTCTC 1 cut(s) 477
BfmI CTRYAG 1 cut(s) 498
BisI GCNGC 1 cut(s) 210
BlpI GCTNAGC 1 cut(s) 31
BlsI GCNGC 1 cut(s) 211
Bme1390I CCNGG 3 cut(s) 254, 307, 527
BmgBI CACGTC 1 cut(s) 337
BmgT120I GGNCC 1 cut(s) 71
BmrFI CCNGG 3 cut(s) 254, 307, 527
BmsI GCATC 1 cut(s) 346
BplI GAGNNNNNCTC 2 cut(s) 392, 424
BpmI CTGGAG 1 cut(s) 477
Bpu10I CCTNAGC 2 cut(s) 85, 360
Bpu1102I GCTNAGC 1 cut(s) 31
BpuEI CTTGAG 1 cut(s) 326
BpuMI CCSGG 1 cut(s) 527
BsaJI CCNNGG 2 cut(s) 148, 525
Bse1I ACTGG 1 cut(s) 460
Bse3DI GCAATG 2 cut(s) 10, 440
BseBI CCWGG 2 cut(s) 254, 307
BseDI CCNNGG 2 cut(s) 148, 525
BseGI GGATG 1 cut(s) 315
BseMI GCAATG 2 cut(s) 10, 440
BseMII CTCAG 3 cut(s) 99, 249, 374
BseNI ACTGG 1 cut(s) 460
BseXI GCAGC 1 cut(s) 221
BshFI GGCC 2 cut(s) 72, 482
BsiHKAI GWGCWC 3 cut(s) 250, 342, 463
BsiSI CCGG 1 cut(s) 527
BsmAI GTCTC 1 cut(s) 477
BsnI GGCC 2 cut(s) 72, 482
Bsp1286I GDGCHC 4 cut(s) 170, 250, 342, 463
Bsp143I GATC 1 cut(s) 504
Bsp1720I GCTNAGC 1 cut(s) 31
BspACI CCGC 1 cut(s) 120
BspANI GGCC 2 cut(s) 72, 482
BspCNI CTCAG 3 cut(s) 98, 250, 373
BspPI GGATC 1 cut(s) 499
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 10, 440
BsrI ACTGG 1 cut(s) 460
BssECI CCNNGG 2 cut(s) 148, 525
BssMI GATC 1 cut(s) 504
BssNAI GTATAC 1 cut(s) 522
BssT1I CCWWGG 1 cut(s) 148
Bst1107I GTATAC 1 cut(s) 522
Bst2UI CCWGG 2 cut(s) 254, 307
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 250, 463
BstDEI CTNAG 4 cut(s) 31, 85, 258, 360
BstF5I GGATG 1 cut(s) 315
BstHHI GCGC 1 cut(s) 270
BstKTI GATC 1 cut(s) 507
BstMAI GTCTC 1 cut(s) 477
BstMBI GATC 1 cut(s) 504
BstMWI GCNNNNNNNGC 1 cut(s) 254
BstNI CCWGG 2 cut(s) 254, 307
BstSCI CCNGG 3 cut(s) 252, 305, 525
BstSFI CTRYAG 1 cut(s) 498
BstV1I GCAGC 1 cut(s) 221
BstX2I RGATCY 1 cut(s) 504
BstYI RGATCY 1 cut(s) 504
BstZ17I GTATAC 1 cut(s) 522
BsuRI GGCC 2 cut(s) 72, 482
BtrI CACGTC 1 cut(s) 337
BtsCI GGATG 1 cut(s) 315
BtsI GCAGTG 2 cut(s) 432, 543
BtsIMutI CAGTG 2 cut(s) 432, 543
Cac8I GCNNGC 2 cut(s) 250, 463
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 270
Cfr13I GGNCC 1 cut(s) 71
Csp6I GTAC 1 cut(s) 78
CviQI GTAC 1 cut(s) 78
DdeI CTNAG 4 cut(s) 31, 85, 258, 360
DpnI GATC 1 cut(s) 506
DpnII GATC 1 cut(s) 504
EaeI YGGCCR 1 cut(s) 480
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 248
Eco130I CCWWGG 1 cut(s) 148
Eco24I GRGCYC 2 cut(s) 170, 250
Eco53kI GAGCTC 1 cut(s) 248
Eco57I CTGAAG 1 cut(s) 387
EcoICRI GAGCTC 1 cut(s) 248
EcoRII CCWGG 2 cut(s) 252, 305
EcoT14I CCWWGG 1 cut(s) 148
EcoT38I GRGCYC 2 cut(s) 170, 250
ErhI CCWWGG 1 cut(s) 148
FaiI YATR 3 cut(s) 406, 447, 522
FalI AAGNNNNNCTT 2 cut(s) 360, 392
FblI GTMKAC 1 cut(s) 521
Fnu4HI GCNGC 1 cut(s) 210
FokI GGATG 1 cut(s) 322
FriOI GRGCYC 2 cut(s) 170, 250
Fsp4HI GCNGC 1 cut(s) 210
GlaI GCGC 1 cut(s) 269
GluI GCNGC 1 cut(s) 210
GsuI CTGGAG 1 cut(s) 477
HaeIII GGCC 2 cut(s) 72, 482
HapII CCGG 1 cut(s) 527
HhaI GCGC 1 cut(s) 270
Hin6I GCGC 1 cut(s) 268
HinP1I GCGC 1 cut(s) 268
HindIII AAGCTT 2 cut(s) 327, 389
HinfI GANTC 2 cut(s) 230, 533
HpaII CCGG 1 cut(s) 527
Hpy166II GTNNAC 1 cut(s) 522
Hpy188I TCNGA 3 cut(s) 177, 295, 538
Hpy188III TCNNGA 4 cut(s) 134, 285, 343, 474
Hpy8I GTNNAC 1 cut(s) 522
Hpy99I CGWCG 1 cut(s) 192
HpyAV CCTTC 6 cut(s) 47, 51, 114, 310, 424, 487
HpyCH4IV ACGT 1 cut(s) 336
HpyCH4V TGCA 1 cut(s) 445
HpyF10VI GCNNNNNNNGC 1 cut(s) 254
HpyF3I CTNAG 4 cut(s) 31, 85, 258, 360
HpySE526I ACGT 1 cut(s) 336
HspAI GCGC 1 cut(s) 268
Kzo9I GATC 1 cut(s) 504
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 217, 458
Lsp1109I GCAGC 1 cut(s) 221
LweI GCATC 1 cut(s) 346
MaeII ACGT 1 cut(s) 336
MaeIII GTNAC 1 cut(s) 541
MalI GATC 1 cut(s) 506
MboI GATC 1 cut(s) 504
MboII GAAGA 7 cut(s) 12, 15, 133, 325, 358, 380, 498
MfeI CAATTG 1 cut(s) 381
MflI RGATCY 1 cut(s) 504
MhlI GDGCHC 4 cut(s) 170, 250, 342, 463
MlsI TGGCCA 1 cut(s) 482
MluCI AATT 2 cut(s) 47, 381
MluNI TGGCCA 1 cut(s) 482
MmeI TCCRAC 2 cut(s) 239, 415
MnlI CCTC 7 cut(s) 74, 94, 119, 137, 171, 199, 394
Mox20I TGGCCA 1 cut(s) 482
MroXI GAANNNNTTC 1 cut(s) 372
MscI TGGCCA 1 cut(s) 482
Msp20I TGGCCA 1 cut(s) 482
MspI CCGG 1 cut(s) 527
MspR9I CCNGG 3 cut(s) 254, 307, 527
MunI CAATTG 1 cut(s) 381
MvaI CCWGG 2 cut(s) 254, 307
MwoI GCNNNNNNNGC 1 cut(s) 254
NciI CCSGG 1 cut(s) 527
NdeII GATC 1 cut(s) 504
NmuCI GTSAC 1 cut(s) 541
PciSI GCTCTTC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 372
PfeI GAWTC 2 cut(s) 230, 533
PkrI GCNGC 1 cut(s) 211
Psp124BI GAGCTC 1 cut(s) 250
Psp6I CCWGG 2 cut(s) 252, 305
PspGI CCWGG 2 cut(s) 252, 305
PspPI GGNCC 1 cut(s) 71
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 1 cut(s) 504
RsaI GTAC 1 cut(s) 79
RsaNI GTAC 1 cut(s) 78
SacI GAGCTC 1 cut(s) 250
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 1 cut(s) 210
Sau3AI GATC 1 cut(s) 504
Sau96I GGNCC 1 cut(s) 71
ScrFI CCNGG 3 cut(s) 254, 307, 527
SduI GDGCHC 4 cut(s) 170, 250, 342, 463
SfaNI GCATC 1 cut(s) 346
SfcI CTRYAG 1 cut(s) 498
SmlI CTYRAG 1 cut(s) 341
SmoI CTYRAG 1 cut(s) 341
Sse9I AATT 2 cut(s) 47, 381
SsiI CCGC 1 cut(s) 120
SstI GAGCTC 1 cut(s) 250
StyD4I CCNGG 3 cut(s) 252, 305, 525
StyI CCWWGG 1 cut(s) 148
TaiI ACGT 1 cut(s) 339
TaqI TCGA 1 cut(s) 475
TasI AATT 2 cut(s) 47, 381
TatI WGTACW 1 cut(s) 77
TfiI GAWTC 2 cut(s) 230, 533
TscAI CASTG 2 cut(s) 432, 550
TseFI GTSAC 1 cut(s) 541
TseI GCWGC 1 cut(s) 209
Tsp45I GTSAC 1 cut(s) 541
TspDTI ATGAA 1 cut(s) 326
TspRI CASTG 2 cut(s) 432, 550
XapI RAATTY 1 cut(s) 47
XmiI GTMKAC 1 cut(s) 521
XmnI GAANNNNTTC 1 cut(s) 372
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.