RLG00000033418

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr7
OG0001972
Physical Location & Seq
Forward (+)
26930931 .. 26932938
2008 bp
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UTR
Exon/CDS
Intron
RLM00000033418

Sequence Viewer

Length: 555 bp
ATGGCTCTTCATTGCCTATCTTCTTCCTGCTTGGCCCTTCCTTCCAAGTTCGGCAACCTTTCCCTCAATGCCCCTTCTACTTCTTCAGCTTCCACCCATTTCCCTTCTCTCTCTTTCTCCGCGAACCTCCTCTCTCACGATTTCTTCTCCAAAGGGTATTTGTGTTTGAGCCCAAGTCAGAGGTCAGTTCGTCGCTCTGTGGTTTGTGAGGCAGCTCCAACCAAGAAGCCTGATTCTGCTGAAAAGAGAGCTCGCCAGGCTGAGAAAAGGCGCATTTACAACAAGTCCAGAAAGTCTGAAATCAAAACCAGGATGAAGAAGGTGTTGGAAGCTTTAGACGTGCTCAAGAAGAAACCAGATGCTCAGGCTGAAGAAGTTCTTTCAATTGAGAAGCTTATCGGCGAGGCATACTCCATCATTGACAAAGCAGTGAAGGTTGGAACATTGCATAGGAATACTGGAGCACGCAGAAAGTCTCGATTGGCCAGAAGAAAGAAGGCTGTAGAGATCCAAAAAGGCTGGTATACCCCGGCTGATTCTGAAGTTACTGCCTGA

Protein Analysis

185

Amino Acids

20.39

Weight (kDa)

10.11

Isoelectric Point (pI)

57.02

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 78 - 164 6.2e-22 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 524
AccII CGCG 1 cut(s) 122
AciI CCGC 1 cut(s) 120
AclWI GGATC 1 cut(s) 502
AcoI YGGCCR 1 cut(s) 483
AcuI CTGAAG 2 cut(s) 69, 390
AgsI TTSAA 1 cut(s) 384
AjiI CACGTC 1 cut(s) 340
AjnI CCWGG 2 cut(s) 255, 308
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 5 cut(s) 89, 215, 251, 332, 394
AluI AGCT 5 cut(s) 89, 215, 251, 332, 394
Alw21I GWGCWC 3 cut(s) 253, 345, 466
Alw26I GTCTC 1 cut(s) 480
AlwI GGATC 1 cut(s) 502
AoxI GGCC 2 cut(s) 33, 483
ApeKI GCWGC 1 cut(s) 212
Asp700I GAANNNNTTC 1 cut(s) 375
AspLEI GCGC 1 cut(s) 273
AspS9I GGNCC 1 cut(s) 34
AsuC2I CCSGG 1 cut(s) 530
BalI TGGCCA 1 cut(s) 485
BanII GRGCYC 2 cut(s) 173, 253
Bbv12I GWGCWC 3 cut(s) 253, 345, 466
BbvI GCAGC 1 cut(s) 224
BccI CCATC 1 cut(s) 422
BciT130I CCWGG 2 cut(s) 257, 310
BcnI CCSGG 1 cut(s) 530
BcoDI GTCTC 1 cut(s) 480
BfmI CTRYAG 1 cut(s) 501
BisI GCNGC 1 cut(s) 213
BlsI GCNGC 1 cut(s) 214
Bme1390I CCNGG 3 cut(s) 257, 310, 530
BmgBI CACGTC 1 cut(s) 340
BmgT120I GGNCC 1 cut(s) 34
BmrFI CCNGG 3 cut(s) 257, 310, 530
BmsI GCATC 1 cut(s) 349
BplI GAGNNNNNCTC 2 cut(s) 395, 427
BpmI CTGGAG 1 cut(s) 480
Bpu10I CCTNAGC 1 cut(s) 363
BpuEI CTTGAG 1 cut(s) 329
BpuMI CCSGG 1 cut(s) 530
BsaJI CCNNGG 1 cut(s) 528
Bse1I ACTGG 1 cut(s) 463
Bse3DI GCAATG 2 cut(s) 10, 443
BseBI CCWGG 2 cut(s) 257, 310
BseDI CCNNGG 1 cut(s) 528
BseGI GGATG 1 cut(s) 318
BseMI GCAATG 2 cut(s) 10, 443
BseMII CTCAG 2 cut(s) 252, 377
BseNI ACTGG 1 cut(s) 463
BseRI GAGGAG 1 cut(s) 119
BseXI GCAGC 1 cut(s) 224
Bsh1236I CGCG 1 cut(s) 122
BshFI GGCC 2 cut(s) 35, 485
BsiHKAI GWGCWC 3 cut(s) 253, 345, 466
BsiSI CCGG 1 cut(s) 530
BsmAI GTCTC 1 cut(s) 480
BsnI GGCC 2 cut(s) 35, 485
Bsp1286I GDGCHC 4 cut(s) 173, 253, 345, 466
Bsp143I GATC 1 cut(s) 507
BspACI CCGC 1 cut(s) 120
BspANI GGCC 2 cut(s) 35, 485
BspCNI CTCAG 2 cut(s) 253, 376
BspFNI CGCG 1 cut(s) 122
BspPI GGATC 1 cut(s) 502
BspQI GCTCTTC 1 cut(s) 12
BsrDI GCAATG 2 cut(s) 10, 443
BsrI ACTGG 1 cut(s) 463
BssECI CCNNGG 1 cut(s) 528
BssMI GATC 1 cut(s) 507
BssNAI GTATAC 1 cut(s) 525
Bst1107I GTATAC 1 cut(s) 525
Bst2UI CCWGG 2 cut(s) 257, 310
Bst6I CTCTTC 1 cut(s) 12
BstC8I GCNNGC 2 cut(s) 253, 466
BstDEI CTNAG 2 cut(s) 261, 363
BstF5I GGATG 1 cut(s) 318
BstFNI CGCG 1 cut(s) 122
BstHHI GCGC 1 cut(s) 273
BstKTI GATC 1 cut(s) 510
BstMAI GTCTC 1 cut(s) 480
BstMBI GATC 1 cut(s) 507
BstMWI GCNNNNNNNGC 1 cut(s) 257
BstNI CCWGG 2 cut(s) 257, 310
BstSCI CCNGG 3 cut(s) 255, 308, 528
BstSFI CTRYAG 1 cut(s) 501
BstUI CGCG 1 cut(s) 122
BstV1I GCAGC 1 cut(s) 224
BstX2I RGATCY 1 cut(s) 507
BstYI RGATCY 1 cut(s) 507
BstZ17I GTATAC 1 cut(s) 525
BsuRI GGCC 2 cut(s) 35, 485
BtrI CACGTC 1 cut(s) 340
BtsCI GGATG 1 cut(s) 318
BtsI GCAGTG 1 cut(s) 435
BtsIMutI CAGTG 1 cut(s) 435
Cac8I GCNNGC 2 cut(s) 253, 466
CfoI GCGC 1 cut(s) 273
Cfr13I GGNCC 1 cut(s) 34
DdeI CTNAG 2 cut(s) 261, 363
DpnI GATC 1 cut(s) 509
DpnII GATC 1 cut(s) 507
EaeI YGGCCR 1 cut(s) 483
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Ecl136II GAGCTC 1 cut(s) 251
Eco24I GRGCYC 2 cut(s) 173, 253
Eco53kI GAGCTC 1 cut(s) 251
Eco57I CTGAAG 2 cut(s) 69, 390
EcoICRI GAGCTC 1 cut(s) 251
EcoRII CCWGG 2 cut(s) 255, 308
EcoT38I GRGCYC 2 cut(s) 173, 253
FaiI YATR 3 cut(s) 409, 450, 525
FalI AAGNNNNNCTT 2 cut(s) 363, 395
FblI GTMKAC 1 cut(s) 524
Fnu4HI GCNGC 1 cut(s) 213
FokI GGATG 1 cut(s) 325
FriOI GRGCYC 2 cut(s) 173, 253
Fsp4HI GCNGC 1 cut(s) 213
GlaI GCGC 1 cut(s) 272
GluI GCNGC 1 cut(s) 213
GsuI CTGGAG 1 cut(s) 480
HaeIII GGCC 2 cut(s) 35, 485
HapII CCGG 1 cut(s) 530
HhaI GCGC 1 cut(s) 273
Hin6I GCGC 1 cut(s) 271
HinP1I GCGC 1 cut(s) 271
HindIII AAGCTT 2 cut(s) 330, 392
HinfI GANTC 2 cut(s) 233, 536
HpaII CCGG 1 cut(s) 530
Hpy166II GTNNAC 1 cut(s) 525
Hpy188I TCNGA 3 cut(s) 180, 298, 541
Hpy188III TCNNGA 4 cut(s) 137, 288, 346, 477
Hpy8I GTNNAC 1 cut(s) 525
Hpy99I CGWCG 1 cut(s) 195
HpyAV CCTTC 7 cut(s) 47, 51, 84, 114, 313, 427, 490
HpyCH4IV ACGT 1 cut(s) 339
HpyCH4V TGCA 1 cut(s) 448
HpyF10VI GCNNNNNNNGC 1 cut(s) 257
HpyF3I CTNAG 2 cut(s) 261, 363
HpySE526I ACGT 1 cut(s) 339
HspAI GCGC 1 cut(s) 271
Kzo9I GATC 1 cut(s) 507
LguI GCTCTTC 1 cut(s) 12
LmnI GCTCC 2 cut(s) 220, 461
Lsp1109I GCAGC 1 cut(s) 224
LweI GCATC 1 cut(s) 349
MaeII ACGT 1 cut(s) 339
MaeIII GTNAC 1 cut(s) 544
MalI GATC 1 cut(s) 509
MboI GATC 1 cut(s) 507
MboII GAAGA 8 cut(s) 12, 15, 75, 136, 328, 361, 383, 501
MfeI CAATTG 1 cut(s) 384
MflI RGATCY 1 cut(s) 507
MhlI GDGCHC 4 cut(s) 173, 253, 345, 466
MlsI TGGCCA 1 cut(s) 485
MluCI AATT 1 cut(s) 384
MluNI TGGCCA 1 cut(s) 485
MmeI TCCRAC 3 cut(s) 242, 306, 418
MnlI CCTC 6 cut(s) 74, 137, 140, 174, 202, 397
Mox20I TGGCCA 1 cut(s) 485
MroXI GAANNNNTTC 1 cut(s) 375
MscI TGGCCA 1 cut(s) 485
Msp20I TGGCCA 1 cut(s) 485
MspI CCGG 1 cut(s) 530
MspR9I CCNGG 3 cut(s) 257, 310, 530
MunI CAATTG 1 cut(s) 384
MvaI CCWGG 2 cut(s) 257, 310
MvnI CGCG 1 cut(s) 122
MwoI GCNNNNNNNGC 1 cut(s) 257
NciI CCSGG 1 cut(s) 530
NdeII GATC 1 cut(s) 507
PciSI GCTCTTC 1 cut(s) 12
PdmI GAANNNNTTC 1 cut(s) 375
PfeI GAWTC 2 cut(s) 233, 536
PkrI GCNGC 1 cut(s) 214
Psp124BI GAGCTC 1 cut(s) 253
Psp6I CCWGG 2 cut(s) 255, 308
PspGI CCWGG 2 cut(s) 255, 308
PspPI GGNCC 1 cut(s) 34
PsuI RGATCY 1 cut(s) 507
SacI GAGCTC 1 cut(s) 253
SapI GCTCTTC 1 cut(s) 12
SatI GCNGC 1 cut(s) 213
Sau3AI GATC 1 cut(s) 507
Sau96I GGNCC 1 cut(s) 34
ScrFI CCNGG 3 cut(s) 257, 310, 530
SduI GDGCHC 4 cut(s) 173, 253, 345, 466
SfaNI GCATC 1 cut(s) 349
SfcI CTRYAG 1 cut(s) 501
SmlI CTYRAG 1 cut(s) 344
SmoI CTYRAG 1 cut(s) 344
Sse9I AATT 1 cut(s) 384
SsiI CCGC 1 cut(s) 120
SstI GAGCTC 1 cut(s) 253
StyD4I CCNGG 3 cut(s) 255, 308, 528
TaiI ACGT 1 cut(s) 342
TaqI TCGA 1 cut(s) 478
TasI AATT 1 cut(s) 384
TfiI GAWTC 2 cut(s) 233, 536
TscAI CASTG 1 cut(s) 435
TseI GCWGC 1 cut(s) 212
TspDTI ATGAA 1 cut(s) 329
TspRI CASTG 1 cut(s) 435
XmiI GTMKAC 1 cut(s) 524
XmnI GAANNNNTTC 1 cut(s) 375
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.