Rorug05G0268500

receptor-like serine threonine-protein kinase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0001972
Physical Location & Seq
Forward (+)
30318151 .. 30318690
540 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0268500.1

Sequence Viewer

Length: 540 bp
ATGAGAATCTCTTTCTCTCTGCCTTCAAACTCCTCCTCGAAGCCAAACCCTATCAAACCCTCAACCTCCTTCGACGACGACGACACCACCAACCCCAACCTAAACGACGACGCATCGAAACAATACATCCACGAATTCACCGCCGCGGAAGAGCCCTCAGCCAAACCCCGCGTGATCCCGCCCATCCCAAACGAATGGCAGCCCGGCAAGAACAACATGAACAGCCTCGAATTCGTGGCCGAGTCTCTCTCAATCGACGGCTCATATTCCGAGATCCCGTACGGCCTCAATCTCCGGCAGAGACCCGAATCCGAGGAGGCGGTGGTTCAGAAATTGAAAGAGGAGTTGAAGGCGTTGCCGGAGATTAGGGGGATGGAGGAGTTTGATGAGAATCCGGTGGAGGGGTTCGGTGCGGCTCTGCTCGCCGGGTACGGGTGGCAACCGGGGCGAGGAATCGGCAAGAACGCAAAGGAAGATGTTGGTGTTGTGGAGGTCCGGCCCAATGTGGGAAGGCAAGGGTTAGGGTTTCGACCGGAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
LRRNT_2 LRR_8 Pkinase_Tyr

Protein Analysis

179

Amino Acids

19.71

Weight (kDa)

4.72

Isoelectric Point (pI)

37.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
G-patch_2 PF12656 120 - 178 2.6e-16 G-patch domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 2 cut(s) 146, 171
AciI CCGC 7 cut(s) 141, 144, 146, 169, 179, 320, 413
AclWI GGATC 2 cut(s) 169, 268
AcoI YGGCCR 1 cut(s) 237
AcsI RAATTY 2 cut(s) 134, 230
AfaI GTAC 2 cut(s) 281, 431
AfiI CCNNNNNNNGG 4 cut(s) 401, 432, 449, 506
AgsI TTSAA 3 cut(s) 27, 337, 349
Alw26I GTCTC 2 cut(s) 249, 295
AlwI GGATC 2 cut(s) 169, 268
AoxI GGCC 3 cut(s) 237, 283, 497
ApeKI GCWGC 1 cut(s) 199
ApoI RAATTY 2 cut(s) 134, 230
AspS9I GGNCC 2 cut(s) 493, 498
AsuC2I CCSGG 3 cut(s) 204, 427, 444
AsuHPI GGTGA 1 cut(s) 130
AvaII GGWCC 1 cut(s) 493
BanII GRGCYC 1 cut(s) 156
BbvCI CCTCAGC 1 cut(s) 157
BbvI GCAGC 1 cut(s) 211
BccI CCATC 2 cut(s) 191, 367
BceAI ACGGC 2 cut(s) 274, 298
BcnI CCSGG 3 cut(s) 204, 427, 444
BcoDI GTCTC 2 cut(s) 249, 295
BisI GCNGC 3 cut(s) 144, 200, 414
BlsI GCNGC 3 cut(s) 145, 201, 415
Bme1390I CCNGG 3 cut(s) 204, 427, 444
Bme18I GGWCC 1 cut(s) 493
BmgT120I GGNCC 2 cut(s) 493, 498
BmrFI CCNGG 3 cut(s) 204, 427, 444
BmsI GCATC 1 cut(s) 122
BplI GAGNNNNNCTC 2 cut(s) 233, 265
Bpu10I CCTNAGC 1 cut(s) 157
BpuMI CCSGG 3 cut(s) 204, 427, 444
BsaBI GATNNNNATC 1 cut(s) 390
BsaI GGTCTC 1 cut(s) 295
BsaJI CCNNGG 3 cut(s) 144, 312, 443
BsaWI WCCGGW 2 cut(s) 394, 532
Bsc4I CCNNNNNNNGG 4 cut(s) 401, 432, 449, 506
Bse8I GATNNNNATC 1 cut(s) 390
BseDI CCNNGG 3 cut(s) 144, 312, 443
BseGI GGATG 3 cut(s) 126, 183, 378
BseJI GATNNNNATC 1 cut(s) 390
BseLI CCNNNNNNNGG 4 cut(s) 401, 432, 449, 506
BseMII CTCAG 1 cut(s) 171
BseRI GAGGAG 5 cut(s) 22, 25, 329, 356, 392
BseXI GCAGC 1 cut(s) 211
Bsh1236I CGCG 2 cut(s) 146, 171
Bsh1285I CGRYCG 1 cut(s) 533
BshFI GGCC 3 cut(s) 239, 285, 499
BsiEI CGRYCG 1 cut(s) 533
BsiSI CCGG 8 cut(s) 204, 295, 359, 395, 426, 443, 496, 533
BsiWI CGTACG 1 cut(s) 279
BslI CCNNNNNNNGG 4 cut(s) 401, 432, 449, 506
BsmAI GTCTC 2 cut(s) 249, 295
BsnI GGCC 3 cut(s) 239, 285, 499
Bso31I GGTCTC 1 cut(s) 295
Bsp1286I GDGCHC 1 cut(s) 156
Bsp143I GATC 2 cut(s) 174, 273
BspACI CCGC 7 cut(s) 141, 144, 146, 169, 179, 320, 413
BspANI GGCC 3 cut(s) 239, 285, 499
BspCNI CTCAG 1 cut(s) 170
BspFNI CGCG 2 cut(s) 146, 171
BspPI GGATC 2 cut(s) 169, 268
BspQI GCTCTTC 1 cut(s) 144
BspTNI GGTCTC 1 cut(s) 295
BssECI CCNNGG 3 cut(s) 144, 312, 443
BssMI GATC 2 cut(s) 174, 273
Bst6I CTCTTC 1 cut(s) 144
BstC8I GCNNGC 1 cut(s) 423
BstDEI CTNAG 1 cut(s) 157
BstDSI CCRYGG 1 cut(s) 144
BstF5I GGATG 3 cut(s) 126, 183, 378
BstFNI CGCG 2 cut(s) 146, 171
BstKTI GATC 2 cut(s) 177, 276
BstMAI GTCTC 2 cut(s) 249, 295
BstMBI GATC 2 cut(s) 174, 273
BstMCI CGRYCG 1 cut(s) 533
BstMWI GCNNNNNNNGC 2 cut(s) 422, 445
BstSCI CCNGG 3 cut(s) 202, 425, 442
BstUI CGCG 2 cut(s) 146, 171
BstV1I GCAGC 1 cut(s) 211
BstX2I RGATCY 1 cut(s) 273
BstXI CCANNNNNNTGG 1 cut(s) 195
BstYI RGATCY 1 cut(s) 273
BsuRI GGCC 3 cut(s) 239, 285, 499
BtgI CCRYGG 1 cut(s) 144
BtsCI GGATG 3 cut(s) 126, 183, 378
Cac8I GCNNGC 1 cut(s) 423
Cfr13I GGNCC 2 cut(s) 493, 498
Cfr42I CCGCGG 1 cut(s) 147
CseI GACGC 1 cut(s) 119
Csp6I GTAC 2 cut(s) 280, 430
CviAII CATG 1 cut(s) 217
CviQI GTAC 2 cut(s) 280, 430
DdeI CTNAG 1 cut(s) 157
DpnI GATC 2 cut(s) 176, 275
DpnII GATC 2 cut(s) 174, 273
EaeI YGGCCR 1 cut(s) 237
Eam1104I CTCTTC 1 cut(s) 144
EarI CTCTTC 1 cut(s) 144
Eco24I GRGCYC 1 cut(s) 156
Eco31I GGTCTC 1 cut(s) 295
Eco47I GGWCC 1 cut(s) 493
EcoRI GAATTC 2 cut(s) 134, 230
EcoT38I GRGCYC 1 cut(s) 156
FaeI CATG 1 cut(s) 220
FaiI YATR 2 cut(s) 218, 265
FatI CATG 1 cut(s) 216
FauI CCCGC 2 cut(s) 176, 186
Fnu4HI GCNGC 3 cut(s) 144, 200, 414
FokI GGATG 3 cut(s) 113, 170, 385
FriOI GRGCYC 1 cut(s) 156
Fsp4HI GCNGC 3 cut(s) 144, 200, 414
GluI GCNGC 3 cut(s) 144, 200, 414
HaeIII GGCC 3 cut(s) 239, 285, 499
HapII CCGG 8 cut(s) 204, 295, 359, 395, 426, 443, 496, 533
HgaI GACGC 1 cut(s) 119
Hin1II CATG 1 cut(s) 220
HinfI GANTC 5 cut(s) 6, 242, 308, 391, 453
HpaII CCGG 8 cut(s) 204, 295, 359, 395, 426, 443, 496, 533
HphI GGTGA 1 cut(s) 130
Hpy188I TCNGA 3 cut(s) 271, 313, 330
Hpy99I CGWCG 6 cut(s) 77, 80, 83, 110, 113, 260
HpyAV CCTTC 4 cut(s) 33, 79, 343, 504
HpyF10VI GCNNNNNNNGC 2 cut(s) 422, 445
HpyF3I CTNAG 1 cut(s) 157
Hsp92II CATG 1 cut(s) 220
KspI CCGCGG 1 cut(s) 147
Kzo9I GATC 2 cut(s) 174, 273
LguI GCTCTTC 1 cut(s) 144
LpnPI CCDG 7 cut(s) 217, 308, 372, 408, 439, 456, 509
Lsp1109I GCAGC 1 cut(s) 211
LweI GCATC 1 cut(s) 122
MalI GATC 2 cut(s) 176, 275
MboI GATC 2 cut(s) 174, 273
MboII GAAGA 2 cut(s) 161, 485
MflI RGATCY 1 cut(s) 273
MhlI GDGCHC 1 cut(s) 156
MluCI AATT 3 cut(s) 134, 230, 332
MlyI GAGTC 1 cut(s) 251
MspA1I CMGCKG 1 cut(s) 146
MspI CCGG 8 cut(s) 204, 295, 359, 395, 426, 443, 496, 533
MspR9I CCNGG 3 cut(s) 204, 427, 444
MvnI CGCG 2 cut(s) 146, 171
MwoI GCNNNNNNNGC 2 cut(s) 422, 445
NciI CCSGG 3 cut(s) 204, 427, 444
NdeII GATC 2 cut(s) 174, 273
NlaIII CATG 1 cut(s) 220
NmeAIII GCCGAG 1 cut(s) 265
PciSI GCTCTTC 1 cut(s) 144
PcsI WCGNNNNNNNCGW 1 cut(s) 78
PfeI GAWTC 4 cut(s) 6, 308, 391, 453
Pfl23II CGTACG 1 cut(s) 279
PkrI GCNGC 3 cut(s) 145, 201, 415
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
PspLI CGTACG 1 cut(s) 279
PspPI GGNCC 2 cut(s) 493, 498
PsuI RGATCY 1 cut(s) 273
RsaI GTAC 2 cut(s) 281, 431
RsaNI GTAC 2 cut(s) 280, 430
SacII CCGCGG 1 cut(s) 147
SapI GCTCTTC 1 cut(s) 144
SatI GCNGC 3 cut(s) 144, 200, 414
Sau3AI GATC 2 cut(s) 174, 273
Sau96I GGNCC 2 cut(s) 493, 498
SchI GAGTC 1 cut(s) 251
ScrFI CCNGG 3 cut(s) 204, 427, 444
SduI GDGCHC 1 cut(s) 156
SetI ASST 3 cut(s) 68, 102, 495
SfaNI GCATC 1 cut(s) 122
Sfr303I CCGCGG 1 cut(s) 147
SgrBI CCGCGG 1 cut(s) 147
SinI GGWCC 1 cut(s) 493
Sse9I AATT 3 cut(s) 134, 230, 332
SsiI CCGC 7 cut(s) 141, 144, 146, 169, 179, 320, 413
StyD4I CCNGG 3 cut(s) 202, 425, 442
TaqI TCGA 6 cut(s) 38, 72, 116, 228, 255, 529
TasI AATT 3 cut(s) 134, 230, 332
TauI GCSGC 2 cut(s) 146, 416
TfiI GAWTC 4 cut(s) 6, 308, 391, 453
TseI GCWGC 1 cut(s) 199
TspDTI ATGAA 1 cut(s) 233
VpaK11BI GGWCC 1 cut(s) 493
XapI RAATTY 2 cut(s) 134, 230
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.