MD03G1232600.v1.1

30S ribosomal protein S20

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0001972
Physical Location & Seq
Reverse (-)
31746377 .. 31748965
2589 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1232600.v1.1.491

Sequence Viewer

Length: 543 bp
ATGGCAGCTGCAGTTCATTGCCTATCCTTCCCTTCAAGATTCAGCAAGCTTTCACTCAATGGCCCTTCTACTTCCTCTGCCACTACTCATTCCACAACTTCCCTCAGTTTCTCAGCCACCCTTTCTCCCAATTTCTTCTCCAAAGGGTACTTGGCTGTGAGCCAACTTCAGAGGCCGGTTCGACATTCCATTGTTTGTGAGGTCGCTACGAAGAAGCCCGACTCTGCTGTGAAGAGAGCTCGGCAGGCCGAAAAGAGGAGGATTTACAACAAATCCCGCAAATCCGAAATTAAAACCCGAATGAAGAAGGTTTTGGAAGCTCTGGATGGGCTGAAGAAGAAGCCGGATGCTGCACCCGAAGAGGTCCTTTCCATTGAGAAGCTCATTGGAGAGGCGTACTCCATTATTGACAAAGCAGTTAAGGTGGGAACTCTGCACAGGAATACAGGAGCGCGGAGAAAGTCTCGACTGGCTAGAAGAAAGAAGGCTGTTGAGGTCCACCATGGCTGGTACACCCCATCTCCAGAAGCTGCTAGTGTCTAA

Protein Analysis

181

Amino Acids

19.86

Weight (kDa)

10.45

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_S20p PF01649 74 - 160 1.9e-22 Ribosomal protein S20
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 454
AciI CCGC 2 cut(s) 277, 454
AcuI CTGAAG 2 cut(s) 152, 353
AfaI GTAC 3 cut(s) 149, 398, 512
AfiI CCNNNNNNNGG 1 cut(s) 255
AgsI TTSAA 1 cut(s) 36
AjuI GAANNNNNNNTTGG 2 cut(s) 296, 328
AluBI AGCT 6 cut(s) 8, 49, 239, 320, 382, 530
AluI AGCT 6 cut(s) 8, 49, 239, 320, 382, 530
Alw21I GWGCWC 1 cut(s) 241
Alw26I GTCTC 1 cut(s) 468
AlwNI CAGNNNCTG 1 cut(s) 530
AoxI GGCC 3 cut(s) 61, 173, 246
ApeKI GCWGC 4 cut(s) 5, 8, 350, 530
AspLEI GCGC 1 cut(s) 454
AspS9I GGNCC 3 cut(s) 62, 364, 496
AvaII GGWCC 2 cut(s) 364, 496
BanII GRGCYC 1 cut(s) 241
Bbv12I GWGCWC 1 cut(s) 241
BbvI GCAGC 3 cut(s) 17, 337, 517
BccI CCATC 2 cut(s) 320, 526
BcoDI GTCTC 1 cut(s) 468
BfaI CTAG 2 cut(s) 474, 534
BfmI CTRYAG 1 cut(s) 9
BisI GCNGC 4 cut(s) 6, 9, 351, 531
BlsI GCNGC 4 cut(s) 7, 10, 352, 532
Bme18I GGWCC 2 cut(s) 364, 496
BmgT120I GGNCC 3 cut(s) 62, 364, 496
BmsI GCATC 1 cut(s) 337
BplI GAGNNNNNCTC 4 cut(s) 383, 415, 448, 480
BpmI CTGGAG 1 cut(s) 507
BsaJI CCNNGG 1 cut(s) 502
BsaXI ACNNNNNCTCC 4 cut(s) 109, 139, 505, 535
Bsc4I CCNNNNNNNGG 1 cut(s) 255
Bse118I RCCGGY 1 cut(s) 175
Bse1I ACTGG 1 cut(s) 474
Bse3DI GCAATG 1 cut(s) 16
BseDI CCNNGG 1 cut(s) 502
BseGI GGATG 2 cut(s) 331, 352
BseLI CCNNNNNNNGG 1 cut(s) 255
BseMI GCAATG 1 cut(s) 16
BseMII CTCAG 2 cut(s) 118, 126
BseNI ACTGG 1 cut(s) 474
BseRI GAGGAG 1 cut(s) 271
BseXI GCAGC 3 cut(s) 17, 337, 517
BsgI GTGCAG 2 cut(s) 336, 419
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 3 cut(s) 63, 175, 248
BsiHKAI GWGCWC 1 cut(s) 241
BsiSI CCGG 2 cut(s) 176, 344
BslI CCNNNNNNNGG 1 cut(s) 255
BsmAI GTCTC 1 cut(s) 468
BsnI GGCC 3 cut(s) 63, 175, 248
Bsp1286I GDGCHC 1 cut(s) 241
Bsp19I CCATGG 1 cut(s) 502
BspACI CCGC 2 cut(s) 277, 454
BspANI GGCC 3 cut(s) 63, 175, 248
BspCNI CTCAG 2 cut(s) 117, 125
BspFNI CGCG 1 cut(s) 454
BspMAI CTGCAG 1 cut(s) 13
BsrDI GCAATG 1 cut(s) 16
BsrFI RCCGGY 1 cut(s) 175
BsrI ACTGG 1 cut(s) 474
BssAI RCCGGY 1 cut(s) 175
BssECI CCNNGG 1 cut(s) 502
BssT1I CCWWGG 1 cut(s) 502
Bst6I CTCTTC 2 cut(s) 227, 354
BstC8I GCNNGC 2 cut(s) 47, 246
BstDEI CTNAG 2 cut(s) 104, 112
BstDSI CCRYGG 1 cut(s) 502
BstF5I GGATG 2 cut(s) 331, 352
BstFNI CGCG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 454
BstMAI GTCTC 1 cut(s) 468
BstMWI GCNNNNNNNGC 1 cut(s) 245
BstSFI CTRYAG 1 cut(s) 9
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 3 cut(s) 17, 337, 517
BsuRI GGCC 3 cut(s) 63, 175, 248
BtgI CCRYGG 1 cut(s) 502
BtsCI GGATG 2 cut(s) 331, 352
Cac8I GCNNGC 2 cut(s) 47, 246
CaiI CAGNNNCTG 1 cut(s) 530
CfoI GCGC 1 cut(s) 454
Cfr10I RCCGGY 1 cut(s) 175
Cfr13I GGNCC 3 cut(s) 62, 364, 496
Csp6I GTAC 3 cut(s) 148, 397, 511
CviAII CATG 1 cut(s) 503
CviQI GTAC 3 cut(s) 148, 397, 511
DdeI CTNAG 2 cut(s) 104, 112
Eam1104I CTCTTC 2 cut(s) 227, 354
EarI CTCTTC 2 cut(s) 227, 354
Ecl136II GAGCTC 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 502
Eco24I GRGCYC 1 cut(s) 241
Eco47I GGWCC 2 cut(s) 364, 496
Eco53kI GAGCTC 1 cut(s) 239
Eco57I CTGAAG 2 cut(s) 152, 353
EcoICRI GAGCTC 1 cut(s) 239
EcoO109I RGGNCCY 1 cut(s) 364
EcoT14I CCWWGG 1 cut(s) 502
EcoT38I GRGCYC 1 cut(s) 241
ErhI CCWWGG 1 cut(s) 502
FaeI CATG 1 cut(s) 506
FaiI YATR 1 cut(s) 504
FalI AAGNNNNNCTT 2 cut(s) 351, 383
FatI CATG 1 cut(s) 502
FauI CCCGC 1 cut(s) 284
Fnu4HI GCNGC 4 cut(s) 6, 9, 351, 531
FokI GGATG 2 cut(s) 338, 359
FriOI GRGCYC 1 cut(s) 241
Fsp4HI GCNGC 4 cut(s) 6, 9, 351, 531
FspBI CTAG 2 cut(s) 474, 534
GlaI GCGC 1 cut(s) 453
GluI GCNGC 4 cut(s) 6, 9, 351, 531
GsuI CTGGAG 1 cut(s) 507
HaeIII GGCC 3 cut(s) 63, 175, 248
HapII CCGG 2 cut(s) 176, 344
HhaI GCGC 1 cut(s) 454
Hin1II CATG 1 cut(s) 506
Hin6I GCGC 1 cut(s) 452
HinP1I GCGC 1 cut(s) 452
HindIII AAGCTT 1 cut(s) 47
HinfI GANTC 2 cut(s) 39, 221
HpaII CCGG 2 cut(s) 176, 344
Hpy166II GTNNAC 2 cut(s) 499, 513
Hpy188I TCNGA 2 cut(s) 171, 286
Hpy188III TCNNGA 4 cut(s) 36, 323, 465, 524
Hpy8I GTNNAC 2 cut(s) 499, 513
HpyAV CCTTC 5 cut(s) 37, 42, 75, 301, 478
HpyCH4V TGCA 3 cut(s) 11, 353, 436
HpyF10VI GCNNNNNNNGC 1 cut(s) 245
HpyF3I CTNAG 2 cut(s) 104, 112
Hsp92II CATG 1 cut(s) 506
HspAI GCGC 1 cut(s) 452
LmnI GCTCC 1 cut(s) 449
LpnPI CCDG 9 cut(s) 189, 230, 308, 357, 424, 432, 455, 493, 537
Lsp1109I GCAGC 3 cut(s) 17, 337, 517
LweI GCATC 1 cut(s) 337
MaeI CTAG 2 cut(s) 474, 534
MboII GAAGA 8 cut(s) 127, 223, 244, 316, 346, 349, 371, 489
MhlI GDGCHC 1 cut(s) 241
MluCI AATT 2 cut(s) 130, 288
MlyI GAGTC 1 cut(s) 215
MnlI CCTC 9 cut(s) 85, 113, 165, 193, 249, 252, 355, 385, 487
MseI TTAA 2 cut(s) 291, 420
MspA1I CMGCKG 1 cut(s) 8
MspI CCGG 2 cut(s) 176, 344
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 1 cut(s) 245
NcoI CCATGG 1 cut(s) 502
NlaIII CATG 1 cut(s) 506
NmeAIII GCCGAG 1 cut(s) 220
PfeI GAWTC 1 cut(s) 39
PkrI GCNGC 4 cut(s) 7, 10, 352, 532
PleI GAGTC 1 cut(s) 215
PpsI GAGTC 1 cut(s) 215
PpuMI RGGWCCY 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 241
Psp5II RGGWCCY 1 cut(s) 364
PspPI GGNCC 3 cut(s) 62, 364, 496
PspPPI RGGWCCY 1 cut(s) 364
PstI CTGCAG 1 cut(s) 13
PstNI CAGNNNCTG 1 cut(s) 530
PvuII CAGCTG 1 cut(s) 8
RsaI GTAC 3 cut(s) 149, 398, 512
RsaNI GTAC 3 cut(s) 148, 397, 511
SacI GAGCTC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 291, 420
SatI GCNGC 4 cut(s) 6, 9, 351, 531
Sau96I GGNCC 3 cut(s) 62, 364, 496
SchI GAGTC 1 cut(s) 215
SduI GDGCHC 1 cut(s) 241
SfaNI GCATC 1 cut(s) 337
SfcI CTRYAG 1 cut(s) 9
SinI GGWCC 2 cut(s) 364, 496
Sse9I AATT 2 cut(s) 130, 288
SsiI CCGC 2 cut(s) 277, 454
SspMI CTAG 2 cut(s) 474, 534
SstI GAGCTC 1 cut(s) 241
StyI CCWWGG 1 cut(s) 502
TaqI TCGA 2 cut(s) 181, 466
TasI AATT 2 cut(s) 130, 288
TfiI GAWTC 1 cut(s) 39
Tru1I TTAA 2 cut(s) 291, 420
Tru9I TTAA 2 cut(s) 291, 420
TseI GCWGC 4 cut(s) 5, 8, 350, 530
TspDTI ATGAA 2 cut(s) 5, 317
VpaK11BI GGWCC 2 cut(s) 364, 496
XcmI CCANNNNNNNNNTGG 1 cut(s) 148
XspI CTAG 2 cut(s) 474, 534
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.