Rroxscaffold_1G00028940

2,3-bisphosphoglycerate-independent phosphoglycerate mutase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0005100
Physical Location & Seq
Forward (+)
37353439 .. 37359014
5576 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00028940.1

Sequence Viewer

Length: 852 bp
ATGTCAAAGATTCTCATTGCTCATCCACTGAATTCCAAGCGGGCCTCTGAAGGGAAGAACATAGCTAATGTTGTCCTTTTACGAGGCTGTGGTATCCGGATTGAGGTTTCTCCATTTGCAAAGAAACATGGGTTATGGCCTTGTATGGTAGCTCCAACAAAAATCATTGCTGGATTGGGCTTATCTCTTGGCATTGATATCCTGGAAGCTCCTGGAGCAACTGGAGACTACAGAACACTTCTAACTTCCAAAACAACTGCAATAGCCAAGGCACTCTCAGCTCCTCTGCAGTCTTGCCCCAATGTGTTTGTGCCAGGGGAAGATGAACACAAACCAGGCCGATCAGATGGCTATGATTTTGGGTTCCTCCACATAAAGGCTATAGATGATGCAGGTCATGATAAGGCAACCATTTTCAAAGTCAAAGGATTGGAAGCTGTGGATCGAGCTATAGGGCAGCTGGCCAGGCTCCTTTGGGAGACTGAATCAACTGGAAAATTTCAGTACTTCCTTTGTGTTACAGGGGACCACTCTACCCCGGTTGAATATGGAGACCACAGCTTTGAGCCAGTCCCATTCACAATATGTTCATTGAAAGATTTTGTGGGTGTTGTAGGTGTGGAAACGATTTTGGGTGGTTCGCTTGATCCATTTCCTCTTCCATCCGTAAAAGAGGGTGAATATCCAACAGATGTGGAGATGGAACGGGAGCCCACCAAACAGCCTCGAGCTTTCAACGGTGATTCAGTTTGTGAGTTTAATGAGATAGCGGCAGCAAGGGGATGTCTTGGACGTTTTTCTGGTGGTGAGATGATGGGAGTAATAAAGAATTTCCTGGAGCTAGAAGCGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Metalloenzyme PhosphMutase

Protein Analysis

283

Amino Acids

30.48

Weight (kDa)

5.56

Isoelectric Point (pI)

36.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Metalloenzyme PF01676 116 - 197 7.4e-15 Metalloenzyme superfamily
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 383
AccIII TCCGGA 1 cut(s) 96
AciI CCGC 2 cut(s) 40, 770
AclWI GGATC 2 cut(s) 450, 641
AcoI YGGCCR 1 cut(s) 462
AcsI RAATTY 3 cut(s) 31, 497, 829
AcuI CTGAAG 1 cut(s) 69
AfaI GTAC 1 cut(s) 506
AfiI CCNNNNNNNGG 3 cut(s) 51, 103, 376
AgsI TTSAA 4 cut(s) 418, 545, 595, 736
AjnI CCWGG 6 cut(s) 201, 211, 313, 334, 464, 834
Alw26I GTCTC 3 cut(s) 219, 473, 546
AlwI GGATC 2 cut(s) 450, 641
Ama87I CYCGRG 1 cut(s) 726
Aor13HI TCCGGA 1 cut(s) 96
AoxI GGCC 4 cut(s) 42, 137, 337, 462
ApeKI GCWGC 2 cut(s) 457, 773
ApoI RAATTY 3 cut(s) 31, 497, 829
ArsI GACNNNNNNTTYG 2 cut(s) 545, 577
AspS9I GGNCC 2 cut(s) 42, 526
AsuC2I CCSGG 1 cut(s) 539
AsuHPI GGTGA 3 cut(s) 689, 752, 818
AvaI CYCGRG 1 cut(s) 726
AvaII GGWCC 1 cut(s) 526
BalI TGGCCA 1 cut(s) 464
BanII GRGCYC 1 cut(s) 714
BbvI GCAGC 2 cut(s) 469, 785
BccI CCATC 4 cut(s) 341, 670, 694, 808
BciT130I CCWGG 6 cut(s) 203, 213, 315, 336, 466, 836
BciVI GTATCC 1 cut(s) 104
BcnI CCSGG 1 cut(s) 539
BcoDI GTCTC 3 cut(s) 219, 473, 546
BfaI CTAG 1 cut(s) 842
BfmI CTRYAG 4 cut(s) 229, 287, 381, 450
BfuAI ACCTGC 1 cut(s) 383
BfuI GTATCC 1 cut(s) 104
BisI GCNGC 3 cut(s) 458, 771, 774
BlsI GCNGC 3 cut(s) 459, 772, 775
BmcAI AGTACT 1 cut(s) 506
Bme1390I CCNGG 7 cut(s) 203, 213, 315, 336, 466, 539, 836
Bme18I GGWCC 1 cut(s) 526
BmeT110I CYCGRG 1 cut(s) 726
BmgT120I GGNCC 2 cut(s) 42, 526
BmiI GGNNCC 4 cut(s) 365, 470, 527, 711
BmrFI CCNGG 7 cut(s) 203, 213, 315, 336, 466, 539, 836
BmsI GCATC 1 cut(s) 379
BpmI CTGGAG 2 cut(s) 234, 243
BpuMI CCSGG 1 cut(s) 539
BsaI GGTCTC 1 cut(s) 546
BsaJI CCNNGG 3 cut(s) 267, 314, 537
BsaWI WCCGGW 1 cut(s) 96
Bsc4I CCNNNNNNNGG 3 cut(s) 51, 103, 376
Bse1I ACTGG 3 cut(s) 226, 496, 569
Bse3DI GCAATG 2 cut(s) 15, 165
BseAI TCCGGA 1 cut(s) 96
BseBI CCWGG 6 cut(s) 203, 213, 315, 336, 466, 836
BseDI CCNNGG 3 cut(s) 267, 314, 537
BseGI GGATG 3 cut(s) 22, 662, 788
BseLI CCNNNNNNNGG 3 cut(s) 51, 103, 376
BseMI GCAATG 2 cut(s) 15, 165
BseMII CTCAG 1 cut(s) 291
BseNI ACTGG 3 cut(s) 226, 496, 569
BseRI GAGGAG 1 cut(s) 273
BseXI GCAGC 2 cut(s) 469, 785
BshFI GGCC 4 cut(s) 44, 139, 339, 464
BsiHKCI CYCGRG 1 cut(s) 726
BsiSI CCGG 2 cut(s) 97, 539
BslFI GGGAC 2 cut(s) 539, 557
BslI CCNNNNNNNGG 3 cut(s) 51, 103, 376
BsmAI GTCTC 3 cut(s) 219, 473, 546
BsmFI GGGAC 2 cut(s) 539, 557
BsnI GGCC 4 cut(s) 44, 139, 339, 464
Bso31I GGTCTC 1 cut(s) 546
BsoBI CYCGRG 1 cut(s) 726
Bsp1286I GDGCHC 1 cut(s) 714
Bsp13I TCCGGA 1 cut(s) 96
Bsp143I GATC 3 cut(s) 341, 442, 646
BspACI CCGC 2 cut(s) 40, 770
BspANI GGCC 4 cut(s) 44, 139, 339, 464
BspCNI CTCAG 1 cut(s) 290
BspEI TCCGGA 1 cut(s) 96
BspHI TCATGA 1 cut(s) 397
BspLI GGNNCC 4 cut(s) 365, 470, 527, 711
BspMAI CTGCAG 1 cut(s) 291
BspMI ACCTGC 1 cut(s) 383
BspPI GGATC 2 cut(s) 450, 641
BspTNI GGTCTC 1 cut(s) 546
BsrDI GCAATG 2 cut(s) 15, 165
BsrI ACTGG 3 cut(s) 226, 496, 569
BssECI CCNNGG 3 cut(s) 267, 314, 537
BssMI GATC 3 cut(s) 341, 442, 646
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 6 cut(s) 203, 213, 315, 336, 466, 836
Bst4CI ACNGT 1 cut(s) 740
Bst6I CTCTTC 1 cut(s) 663
BstC8I GCNNGC 2 cut(s) 42, 462
BstDEI CTNAG 1 cut(s) 277
BstF5I GGATG 3 cut(s) 22, 662, 788
BstKTI GATC 3 cut(s) 344, 445, 649
BstMAI GTCTC 3 cut(s) 219, 473, 546
BstMBI GATC 3 cut(s) 341, 442, 646
BstMWI GCNNNNNNNGC 3 cut(s) 215, 278, 466
BstNI CCWGG 6 cut(s) 203, 213, 315, 336, 466, 836
BstSCI CCNGG 7 cut(s) 201, 211, 313, 334, 464, 537, 834
BstSFI CTRYAG 4 cut(s) 229, 287, 381, 450
BstV1I GCAGC 2 cut(s) 469, 785
BsuI GTATCC 1 cut(s) 104
BsuRI GGCC 4 cut(s) 44, 139, 339, 464
BtsCI GGATG 3 cut(s) 22, 662, 788
BtsIMutI CAGTG 1 cut(s) 26
BveI ACCTGC 1 cut(s) 383
Cac8I GCNNGC 2 cut(s) 42, 462
CciI TCATGA 1 cut(s) 397
Cfr13I GGNCC 2 cut(s) 42, 526
Csp6I GTAC 1 cut(s) 505
CspCI CAANNNNNGTGG 2 cut(s) 675, 710
CviAII CATG 2 cut(s) 128, 398
CviQI GTAC 1 cut(s) 505
DdeI CTNAG 1 cut(s) 277
DpnI GATC 3 cut(s) 343, 444, 648
DpnII GATC 3 cut(s) 341, 442, 646
EaeI YGGCCR 1 cut(s) 462
Eam1104I CTCTTC 1 cut(s) 663
EarI CTCTTC 1 cut(s) 663
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 714
Eco31I GGTCTC 1 cut(s) 546
Eco32I GATATC 1 cut(s) 199
Eco47I GGWCC 1 cut(s) 526
Eco57I CTGAAG 1 cut(s) 69
Eco88I CYCGRG 1 cut(s) 726
EcoRI GAATTC 1 cut(s) 31
EcoRII CCWGG 6 cut(s) 201, 211, 313, 334, 464, 834
EcoRV GATATC 1 cut(s) 199
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 714
ErhI CCWWGG 1 cut(s) 267
FaeI CATG 2 cut(s) 131, 401
FaqI GGGAC 2 cut(s) 539, 557
FatI CATG 2 cut(s) 127, 397
FauI CCCGC 1 cut(s) 33
Fnu4HI GCNGC 3 cut(s) 458, 771, 774
FokI GGATG 3 cut(s) 9, 649, 795
FriOI GRGCYC 1 cut(s) 714
Fsp4HI GCNGC 3 cut(s) 458, 771, 774
FspBI CTAG 1 cut(s) 842
GluI GCNGC 3 cut(s) 458, 771, 774
GsuI CTGGAG 2 cut(s) 234, 243
HaeIII GGCC 4 cut(s) 44, 139, 339, 464
HapII CCGG 2 cut(s) 97, 539
Hin1II CATG 2 cut(s) 131, 401
HinfI GANTC 3 cut(s) 10, 485, 743
HpaII CCGG 2 cut(s) 97, 539
HphI GGTGA 3 cut(s) 689, 752, 818
Hpy188I TCNGA 2 cut(s) 49, 346
Hpy188III TCNNGA 2 cut(s) 97, 398
HpyAV CCTTC 1 cut(s) 44
HpyCH4III ACNGT 1 cut(s) 740
HpyCH4IV ACGT 1 cut(s) 793
HpyCH4V TGCA 4 cut(s) 119, 260, 289, 392
HpyF10VI GCNNNNNNNGC 3 cut(s) 215, 278, 466
HpyF3I CTNAG 1 cut(s) 277
HpySE526I ACGT 1 cut(s) 793
Hsp92II CATG 2 cut(s) 131, 401
Kpn2I TCCGGA 1 cut(s) 96
Kzo9I GATC 3 cut(s) 341, 442, 646
LmnI GCTCC 7 cut(s) 157, 214, 215, 286, 474, 709, 838
Lsp1109I GCAGC 2 cut(s) 469, 785
LweI GCATC 1 cut(s) 379
MaeI CTAG 1 cut(s) 842
MaeII ACGT 1 cut(s) 793
MaeIII GTNAC 1 cut(s) 517
MalI GATC 3 cut(s) 343, 444, 648
MboI GATC 3 cut(s) 341, 442, 646
MboII GAAGA 3 cut(s) 67, 332, 650
MhlI GDGCHC 1 cut(s) 714
MlsI TGGCCA 1 cut(s) 464
MluCI AATT 3 cut(s) 31, 497, 829
MluNI TGGCCA 1 cut(s) 464
MmeI TCCRAC 2 cut(s) 179, 710
MnlI CCTC 8 cut(s) 55, 77, 97, 294, 377, 666, 667, 735
Mox20I TGGCCA 1 cut(s) 464
MroI TCCGGA 1 cut(s) 96
MscI TGGCCA 1 cut(s) 464
MseI TTAA 1 cut(s) 759
Msp20I TGGCCA 1 cut(s) 464
MspA1I CMGCKG 1 cut(s) 460
MspI CCGG 2 cut(s) 97, 539
MspR9I CCNGG 7 cut(s) 203, 213, 315, 336, 466, 539, 836
MvaI CCWGG 6 cut(s) 203, 213, 315, 336, 466, 836
MwoI GCNNNNNNNGC 3 cut(s) 215, 278, 466
NciI CCSGG 1 cut(s) 539
NdeII GATC 3 cut(s) 341, 442, 646
NlaIII CATG 2 cut(s) 131, 401
NlaIV GGNNCC 4 cut(s) 365, 470, 527, 711
PaeR7I CTCGAG 1 cut(s) 726
PagI TCATGA 1 cut(s) 397
PfeI GAWTC 3 cut(s) 10, 485, 743
PfoI TCCNGGA 3 cut(s) 201, 211, 834
PkrI GCNGC 3 cut(s) 459, 772, 775
Psp6I CCWGG 6 cut(s) 201, 211, 313, 334, 464, 834
PspGI CCWGG 6 cut(s) 201, 211, 313, 334, 464, 834
PspN4I GGNNCC 4 cut(s) 365, 470, 527, 711
PspPI GGNCC 2 cut(s) 42, 526
PspXI VCTCGAGB 1 cut(s) 726
PstI CTGCAG 1 cut(s) 291
PvuII CAGCTG 1 cut(s) 460
RsaI GTAC 1 cut(s) 506
RsaNI GTAC 1 cut(s) 505
SaqAI TTAA 1 cut(s) 759
SatI GCNGC 3 cut(s) 458, 771, 774
Sau3AI GATC 3 cut(s) 341, 442, 646
Sau96I GGNCC 2 cut(s) 42, 526
ScaI AGTACT 1 cut(s) 506
ScrFI CCNGG 7 cut(s) 203, 213, 315, 336, 466, 539, 836
SduI GDGCHC 1 cut(s) 714
SfaNI GCATC 1 cut(s) 379
SfcI CTRYAG 4 cut(s) 229, 287, 381, 450
Sfr274I CTCGAG 1 cut(s) 726
SinI GGWCC 1 cut(s) 526
SlaI CTCGAG 1 cut(s) 726
SmlI CTYRAG 1 cut(s) 726
SmoI CTYRAG 1 cut(s) 726
Sse9I AATT 3 cut(s) 31, 497, 829
SsiI CCGC 2 cut(s) 40, 770
SspMI CTAG 1 cut(s) 842
StyD4I CCNGG 7 cut(s) 201, 211, 313, 334, 464, 537, 834
StyI CCWWGG 1 cut(s) 267
TaaI ACNGT 1 cut(s) 740
TaiI ACGT 1 cut(s) 796
TaqI TCGA 2 cut(s) 445, 727
TasI AATT 3 cut(s) 31, 497, 829
TatI WGTACW 1 cut(s) 504
TauI GCSGC 1 cut(s) 773
TfiI GAWTC 3 cut(s) 10, 485, 743
Tru1I TTAA 1 cut(s) 759
Tru9I TTAA 1 cut(s) 759
TscAI CASTG 1 cut(s) 33
TseI GCWGC 2 cut(s) 457, 773
TspDTI ATGAA 2 cut(s) 339, 579
TspGWI ACGGA 1 cut(s) 655
TspRI CASTG 1 cut(s) 33
VpaK11BI GGWCC 1 cut(s) 526
XapI RAATTY 3 cut(s) 31, 497, 829
XhoI CTCGAG 1 cut(s) 726
XspI CTAG 1 cut(s) 842
ZrmI AGTACT 1 cut(s) 506
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.