FvH4_5g32280

Ribonuclease H protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0010573
Physical Location & Seq
Forward (+)
23283402 .. 23284684
1283 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g32280.t1

Sequence Viewer

Length: 882 bp
ATGTCAAGTCCGGTCGTGAGAGAGAGGCAATATATTGTTAGCTGCGCATATCAAGAACTGGAAGCCAACCCTGACCTCCTAGGCGAGTTGCCCTGCAACCCTCCGGACATGGTTCCAATCGTATTCGAGCTCTTGAAGACGGTCAGAAGTGGCTTGATTGATGAAGAAGATGATGAAGAGCCACCAGGTTTGCGATTGGAAGATTCTCGTATCCTTCAAGTAGAAATCATCAGGGACGTTGGTTTGTCGCGTCTCAAGGAATCATCTCCAACATCTAGAAGTGAACTCATAGAGAGCTTCGTTCATATTCTTTCCACCAAATTCCATGTTCCAGAAGAACAGCATCAAACGATCATTCATCAAATCTTTAAAGAGGTTGATAGGGATTCTCAGCTCCGCCCCTTGCGGCCTTTTAACGTGATTGTAAACAATGTGACTGTGCAGTTACGGCCTCCTCCTTCCTCTCCAGTTTATTTTGGTGCATTTCTTGGTCTGCTAGACCTCTACACCGAATATTTATCAATGATTACTGATAATGAGGAGGAGGGTTTGGAGATAGTGGGACCTGATAGCTTGGAGGCTAAGGAACAAGAATCATGTCCTATTTGTATGGATGATTTTGAGGAAAAGGAGGATGCAGCCCCAATTGCTCGCTTGCTCTCATGCTTCCATAATCAGAATCAGGTGAGTCTCCATTTGGGGAAGCATAATGCAAGGCAGGGCTGTTCTAAAGGCTGGTTTGCGCAATGGAAGGTTGGAGATGGCAAGAGTGTTCGTATATGGGGCCATAAATGGCTCCCTCTCACTACACCTAGTGCCCTGCAGAGGCAGAATAACAACAATATGGAAATGGTGTCTGATCTTGTGCAATTCTCTGCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF4283 RVT_1

Protein Analysis

294

Amino Acids

33.28

Weight (kDa)

4.8

Isoelectric Point (pI)

63.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 46, 744
AccII CGCG 1 cut(s) 250
AccIII TCCGGA 1 cut(s) 103
AciI CCGC 2 cut(s) 397, 406
AcsI RAATTY 1 cut(s) 320
AdeI CACNNNGTG 1 cut(s) 815
AfiI CCNNNNNNNGG 1 cut(s) 825
AgsI TTSAA 2 cut(s) 136, 218
AjnI CCWGG 1 cut(s) 184
AluBI AGCT 5 cut(s) 42, 130, 297, 394, 573
AluI AGCT 5 cut(s) 42, 130, 297, 394, 573
Alw21I GWGCWC 1 cut(s) 132
Alw26I GTCTC 2 cut(s) 257, 695
Aor13HI TCCGGA 1 cut(s) 103
AoxI GGCC 3 cut(s) 407, 449, 784
ApeKI GCWGC 2 cut(s) 42, 638
ApoI RAATTY 1 cut(s) 320
AspA2I CCTAGG 1 cut(s) 79
AspLEI GCGC 2 cut(s) 47, 745
AspS9I GGNCC 2 cut(s) 563, 784
AsuHPI GGTGA 1 cut(s) 697
AvaII GGWCC 1 cut(s) 563
AvrII CCTAGG 1 cut(s) 79
BaeGI GKGCMC 1 cut(s) 820
BanII GRGCYC 1 cut(s) 132
BbsI GAAGAC 1 cut(s) 143
Bbv12I GWGCWC 1 cut(s) 132
BbvI GCAGC 2 cut(s) 29, 650
BccI CCATC 1 cut(s) 755
BceAI ACGGC 1 cut(s) 464
BciT130I CCWGG 1 cut(s) 186
BciVI GTATCC 1 cut(s) 221
BcoDI GTCTC 2 cut(s) 257, 695
BfaI CTAG 4 cut(s) 80, 276, 497, 813
BfmI CTRYAG 1 cut(s) 821
BfuI GTATCC 1 cut(s) 221
BisI GCNGC 3 cut(s) 43, 407, 639
BlnI CCTAGG 1 cut(s) 79
BlsI GCNGC 3 cut(s) 44, 408, 640
Bme1390I CCNGG 1 cut(s) 186
Bme18I GGWCC 1 cut(s) 563
BmgT120I GGNCC 2 cut(s) 563, 784
BmiI GGNNCC 4 cut(s) 114, 564, 785, 797
BmrFI CCNGG 1 cut(s) 186
BmsI GCATC 2 cut(s) 352, 625
BpiI GAAGAC 1 cut(s) 143
BpmI CTGGAG 1 cut(s) 450
Bpu10I CCTNAGC 1 cut(s) 582
BpuEI CTTGAG 1 cut(s) 239
BsaJI CCNNGG 1 cut(s) 79
BsaWI WCCGGW 2 cut(s) 10, 103
Bsc4I CCNNNNNNNGG 1 cut(s) 825
Bse1I ACTGG 2 cut(s) 63, 467
Bse3DI GCAATG 1 cut(s) 752
BseAI TCCGGA 1 cut(s) 103
BseBI CCWGG 1 cut(s) 186
BseDI CCNNGG 1 cut(s) 79
BseGI GGATG 2 cut(s) 619, 640
BseLI CCNNNNNNNGG 1 cut(s) 825
BseMI GCAATG 1 cut(s) 752
BseMII CTCAG 1 cut(s) 404
BseNI ACTGG 2 cut(s) 63, 467
BseRI GAGGAG 3 cut(s) 444, 554, 557
BseSI GKGCMC 1 cut(s) 820
BseXI GCAGC 2 cut(s) 29, 650
BsgI GTGCAG 1 cut(s) 461
Bsh1236I CGCG 1 cut(s) 250
Bsh1285I CGRYCG 1 cut(s) 15
BshFI GGCC 3 cut(s) 409, 451, 786
BsiEI CGRYCG 1 cut(s) 15
BsiHKAI GWGCWC 1 cut(s) 132
BsiSI CCGG 2 cut(s) 11, 104
BslFI GGGAC 2 cut(s) 248, 576
BslI CCNNNNNNNGG 1 cut(s) 825
BsmAI GTCTC 2 cut(s) 257, 695
BsmBI CGTCTC 1 cut(s) 257
BsmFI GGGAC 2 cut(s) 248, 576
BsnI GGCC 3 cut(s) 409, 451, 786
Bsp1286I GDGCHC 2 cut(s) 132, 820
Bsp13I TCCGGA 1 cut(s) 103
Bsp143I GATC 2 cut(s) 351, 859
BspACI CCGC 2 cut(s) 397, 406
BspANI GGCC 3 cut(s) 409, 451, 786
BspCNI CTCAG 1 cut(s) 403
BspEI TCCGGA 1 cut(s) 103
BspFNI CGCG 1 cut(s) 250
BspLI GGNNCC 4 cut(s) 114, 564, 785, 797
BspMAI CTGCAG 1 cut(s) 825
BspQI GCTCTTC 1 cut(s) 171
BsrDI GCAATG 1 cut(s) 752
BsrI ACTGG 2 cut(s) 63, 467
BssECI CCNNGG 1 cut(s) 79
BssMI GATC 2 cut(s) 351, 859
BssT1I CCWWGG 1 cut(s) 79
Bst2UI CCWGG 1 cut(s) 186
Bst4CI ACNGT 2 cut(s) 142, 439
Bst6I CTCTTC 1 cut(s) 171
BstC8I GCNNGC 2 cut(s) 652, 656
BstDEI CTNAG 2 cut(s) 390, 582
BstF5I GGATG 2 cut(s) 619, 640
BstFNI CGCG 1 cut(s) 250
BstHHI GCGC 2 cut(s) 47, 745
BstKTI GATC 2 cut(s) 354, 862
BstMAI GTCTC 2 cut(s) 257, 695
BstMBI GATC 2 cut(s) 351, 859
BstMCI CGRYCG 1 cut(s) 15
BstMWI GCNNNNNNNGC 2 cut(s) 448, 647
BstNI CCWGG 1 cut(s) 186
BstSCI CCNGG 1 cut(s) 184
BstSFI CTRYAG 1 cut(s) 821
BstSLI GKGCMC 1 cut(s) 820
BstUI CGCG 1 cut(s) 250
BstV1I GCAGC 2 cut(s) 29, 650
BstV2I GAAGAC 1 cut(s) 143
BsuI GTATCC 1 cut(s) 221
BsuRI GGCC 3 cut(s) 409, 451, 786
BtsCI GGATG 2 cut(s) 619, 640
Cac8I GCNNGC 2 cut(s) 652, 656
CfoI GCGC 2 cut(s) 47, 745
Cfr13I GGNCC 2 cut(s) 563, 784
CseI GACGC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 184
CspCI CAANNNNNGTGG 2 cut(s) 171, 206
CviAII CATG 4 cut(s) 109, 326, 597, 663
DdeI CTNAG 2 cut(s) 390, 582
DpnI GATC 2 cut(s) 353, 861
DpnII GATC 2 cut(s) 351, 859
DraI TTTAAA 1 cut(s) 370
DraIII CACNNNGTG 1 cut(s) 815
Eam1104I CTCTTC 1 cut(s) 171
EarI CTCTTC 1 cut(s) 171
EciI GGCGGA 1 cut(s) 386
Ecl136II GAGCTC 1 cut(s) 130
Eco130I CCWWGG 1 cut(s) 79
Eco24I GRGCYC 1 cut(s) 132
Eco47I GGWCC 1 cut(s) 563
Eco53kI GAGCTC 1 cut(s) 130
EcoICRI GAGCTC 1 cut(s) 130
EcoO109I RGGNCCY 1 cut(s) 563
EcoRII CCWGG 1 cut(s) 184
EcoT14I CCWWGG 1 cut(s) 79
EcoT38I GRGCYC 1 cut(s) 132
ErhI CCWWGG 1 cut(s) 79
Esp3I CGTCTC 1 cut(s) 257
FaeI CATG 4 cut(s) 112, 329, 600, 666
FaqI GGGAC 2 cut(s) 248, 576
FatI CATG 4 cut(s) 108, 325, 596, 662
Fnu4HI GCNGC 3 cut(s) 43, 407, 639
FokI GGATG 2 cut(s) 626, 647
FriOI GRGCYC 1 cut(s) 132
Fsp4HI GCNGC 3 cut(s) 43, 407, 639
FspBI CTAG 4 cut(s) 80, 276, 497, 813
FspI TGCGCA 2 cut(s) 46, 744
GlaI GCGC 2 cut(s) 46, 744
GluI GCNGC 3 cut(s) 43, 407, 639
GsuI CTGGAG 1 cut(s) 450
HaeIII GGCC 3 cut(s) 409, 451, 786
HapII CCGG 2 cut(s) 11, 104
HgaI GACGC 1 cut(s) 239
HhaI GCGC 2 cut(s) 47, 745
Hin1II CATG 4 cut(s) 112, 329, 600, 666
Hin6I GCGC 2 cut(s) 45, 743
HinP1I GCGC 2 cut(s) 45, 743
HinfI GANTC 6 cut(s) 203, 260, 386, 593, 679, 688
HpaII CCGG 2 cut(s) 11, 104
HphI GGTGA 1 cut(s) 697
Hpy166II GTNNAC 2 cut(s) 284, 427
Hpy188I TCNGA 3 cut(s) 146, 678, 859
Hpy188III TCNNGA 6 cut(s) 16, 53, 104, 133, 276, 332
Hpy8I GTNNAC 2 cut(s) 284, 427
HpyAV CCTTC 3 cut(s) 224, 468, 745
HpyCH4III ACNGT 2 cut(s) 142, 439
HpyCH4IV ACGT 2 cut(s) 237, 417
HpyCH4V TGCA 7 cut(s) 96, 442, 482, 638, 713, 823, 868
HpyF10VI GCNNNNNNNGC 2 cut(s) 448, 647
HpyF3I CTNAG 2 cut(s) 390, 582
HpySE526I ACGT 2 cut(s) 237, 417
Hsp92II CATG 4 cut(s) 112, 329, 600, 666
HspAI GCGC 2 cut(s) 45, 743
Kpn2I TCCGGA 1 cut(s) 103
Kzo9I GATC 2 cut(s) 351, 859
LguI GCTCTTC 1 cut(s) 171
LmnI GCTCC 2 cut(s) 399, 801
Lsp1109I GCAGC 2 cut(s) 29, 650
LweI GCATC 2 cut(s) 352, 625
MabI ACCWGGT 1 cut(s) 184
MaeI CTAG 4 cut(s) 80, 276, 497, 813
MaeII ACGT 2 cut(s) 237, 417
MaeIII GTNAC 2 cut(s) 433, 444
MalI GATC 2 cut(s) 353, 861
MboI GATC 2 cut(s) 351, 859
MboII GAAGA 6 cut(s) 148, 176, 179, 188, 212, 347
MfeI CAATTG 1 cut(s) 645
MhlI GDGCHC 2 cut(s) 132, 820
MluCI AATT 3 cut(s) 320, 645, 869
MlyI GAGTC 1 cut(s) 697
MmeI TCCRAC 2 cut(s) 293, 736
MroI TCCGGA 1 cut(s) 103
MseI TTAA 2 cut(s) 369, 414
MspI CCGG 2 cut(s) 11, 104
MspR9I CCNGG 1 cut(s) 186
MunI CAATTG 1 cut(s) 645
MvaI CCWGG 1 cut(s) 186
MvnI CGCG 1 cut(s) 250
MwoI GCNNNNNNNGC 2 cut(s) 448, 647
NdeII GATC 2 cut(s) 351, 859
NlaIII CATG 4 cut(s) 112, 329, 600, 666
NlaIV GGNNCC 4 cut(s) 114, 564, 785, 797
NmuCI GTSAC 1 cut(s) 433
NsbI TGCGCA 2 cut(s) 46, 744
PciSI GCTCTTC 1 cut(s) 171
PfeI GAWTC 5 cut(s) 203, 260, 386, 593, 679
PkrI GCNGC 3 cut(s) 44, 408, 640
PleI GAGTC 1 cut(s) 696
PpsI GAGTC 1 cut(s) 696
PpuMI RGGWCCY 1 cut(s) 563
Psp124BI GAGCTC 1 cut(s) 132
Psp5II RGGWCCY 1 cut(s) 563
Psp6I CCWGG 1 cut(s) 184
PspGI CCWGG 1 cut(s) 184
PspN4I GGNNCC 4 cut(s) 114, 564, 785, 797
PspPI GGNCC 2 cut(s) 563, 784
PspPPI RGGWCCY 1 cut(s) 563
PstI CTGCAG 1 cut(s) 825
SacI GAGCTC 1 cut(s) 132
SapI GCTCTTC 1 cut(s) 171
SaqAI TTAA 2 cut(s) 369, 414
SatI GCNGC 3 cut(s) 43, 407, 639
Sau3AI GATC 2 cut(s) 351, 859
Sau96I GGNCC 2 cut(s) 563, 784
SchI GAGTC 1 cut(s) 697
ScrFI CCNGG 1 cut(s) 186
SduI GDGCHC 2 cut(s) 132, 820
SexAI ACCWGGT 1 cut(s) 184
SfaNI GCATC 2 cut(s) 352, 625
SfcI CTRYAG 1 cut(s) 821
SinI GGWCC 1 cut(s) 563
SmlI CTYRAG 1 cut(s) 254
SmoI CTYRAG 1 cut(s) 254
Sse9I AATT 3 cut(s) 320, 645, 869
SsiI CCGC 2 cut(s) 397, 406
SspI AATATT 1 cut(s) 515
SspMI CTAG 4 cut(s) 80, 276, 497, 813
SstI GAGCTC 1 cut(s) 132
StyD4I CCNGG 1 cut(s) 184
StyI CCWWGG 1 cut(s) 79
TaaI ACNGT 2 cut(s) 142, 439
TaiI ACGT 2 cut(s) 240, 420
TaqI TCGA 1 cut(s) 126
TasI AATT 3 cut(s) 320, 645, 869
TauI GCSGC 1 cut(s) 409
TfiI GAWTC 5 cut(s) 203, 260, 386, 593, 679
Tru1I TTAA 2 cut(s) 369, 414
Tru9I TTAA 2 cut(s) 369, 414
TseFI GTSAC 1 cut(s) 433
TseI GCWGC 2 cut(s) 42, 638
Tsp45I GTSAC 1 cut(s) 433
TspDTI ATGAA 4 cut(s) 177, 189, 293, 347
VpaK11BI GGWCC 1 cut(s) 563
XapI RAATTY 1 cut(s) 320
XbaI TCTAGA 1 cut(s) 275
XmaJI CCTAGG 1 cut(s) 79
XspI CTAG 4 cut(s) 80, 276, 497, 813
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.