Rh5BG085300

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5B
OG0010573
Physical Location & Seq
Forward (+)
7390643 .. 7391362
720 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5BG085300.1

Sequence Viewer

Length: 477 bp
ATGTCAACCATCAGAGAAATATTCTTTACTTATTCCGATGCACGGCAAACCAACGAAAACACTGAATCCTACCTCGCCCACTTACTCTTCTTCAATCCACAGGACATAGTTCCCATCAGATTCAAGATTGTGGAGAAATTCAAAAGCGGCTGTGTCTCAGATTCTGAAGATGACGATCCTGTTTCTTACCCAACAGACATTTTCTTCGAAGAGATAGGATCCATAGTTGCAAGAGAAGAAGTCACTCTAGATTATTCTCTCTCGAGTCTCAAGCAAAAATCTGAGAGGAGTTCGCATAAGGAACACTTCGGCTACATTCTTTCCAGATCTTTGGGTGTCTCGGAAGAGTTGCATCCGCCTATCATTGAGAAACTCTTTGAGGTAGTTGATGATGAAGACGCTGTGCCCTTGCTTCCTATTGAAGTGGTTATCACTAACGTGACTTTGGCTTTGACTTCAAGGTGGTTAGAGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

158

Amino Acids

18.11

Weight (kDa)

4.52

Isoelectric Point (pI)

53.61

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 147, 356
AclWI GGATC 3 cut(s) 170, 213, 226
AcsI RAATTY 1 cut(s) 137
AcuI CTGAAG 1 cut(s) 186
AfiI CCNNNNNNNGG 1 cut(s) 42
AgsI TTSAA 5 cut(s) 94, 124, 142, 422, 459
AleI CACNNNNGTG 1 cut(s) 437
Alw26I GTCTC 3 cut(s) 160, 272, 343
AlwI GGATC 3 cut(s) 170, 213, 226
AlwNI CAGNNNCTG 1 cut(s) 164
Ama87I CYCGRG 1 cut(s) 262
ApoI RAATTY 1 cut(s) 137
ArsI GACNNNNNNTTYG 4 cut(s) 188, 220, 433, 465
AsuII TTCGAA 1 cut(s) 207
AvaI CYCGRG 1 cut(s) 262
BaeGI GKGCMC 1 cut(s) 408
BamHI GGATCC 1 cut(s) 218
BbsI GAAGAC 1 cut(s) 402
BccI CCATC 2 cut(s) 17, 122
BceAI ACGGC 1 cut(s) 59
BcoDI GTCTC 3 cut(s) 160, 272, 343
BfaI CTAG 1 cut(s) 248
BglII AGATCT 1 cut(s) 326
BisI GCNGC 1 cut(s) 148
BlsI GCNGC 1 cut(s) 149
BmeT110I CYCGRG 1 cut(s) 262
BmiI GGNNCC 1 cut(s) 220
BmsI GCATC 2 cut(s) 28, 361
BpiI GAAGAC 1 cut(s) 402
Bpu14I TTCGAA 1 cut(s) 207
BpuEI CTTGAG 1 cut(s) 254
BsaBI GATNNNNATC 1 cut(s) 174
Bsc4I CCNNNNNNNGG 1 cut(s) 42
Bse8I GATNNNNATC 1 cut(s) 174
BseGI GGATG 1 cut(s) 352
BseJI GATNNNNATC 1 cut(s) 174
BseLI CCNNNNNNNGG 1 cut(s) 42
BseMII CTCAG 2 cut(s) 171, 273
BseRI GAGGAG 1 cut(s) 301
BseSI GKGCMC 1 cut(s) 408
BsiHKCI CYCGRG 1 cut(s) 262
BslI CCNNNNNNNGG 1 cut(s) 42
BsmAI GTCTC 3 cut(s) 160, 272, 343
BsoBI CYCGRG 1 cut(s) 262
Bsp119I TTCGAA 1 cut(s) 207
Bsp1286I GDGCHC 1 cut(s) 408
Bsp143I GATC 3 cut(s) 175, 218, 326
BspACI CCGC 2 cut(s) 147, 356
BspCNI CTCAG 2 cut(s) 170, 274
BspLI GGNNCC 1 cut(s) 220
BspPI GGATC 3 cut(s) 170, 213, 226
BspT104I TTCGAA 1 cut(s) 207
BssMI GATC 3 cut(s) 175, 218, 326
Bst6I CTCTTC 3 cut(s) 92, 204, 339
BstBI TTCGAA 1 cut(s) 207
BstDEI CTNAG 2 cut(s) 157, 282
BstF5I GGATG 1 cut(s) 352
BstKTI GATC 3 cut(s) 178, 221, 329
BstMAI GTCTC 3 cut(s) 160, 272, 343
BstMBI GATC 3 cut(s) 175, 218, 326
BstSLI GKGCMC 1 cut(s) 408
BstV2I GAAGAC 1 cut(s) 402
BstX2I RGATCY 2 cut(s) 218, 326
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 2 cut(s) 218, 326
BtsCI GGATG 1 cut(s) 352
BtsIMutI CAGTG 1 cut(s) 60
CaiI CAGNNNCTG 1 cut(s) 164
CseI GACGC 1 cut(s) 407
CviJI RGCY 3 cut(s) 150, 312, 449
CviKI_1 RGCY 3 cut(s) 150, 312, 449
DdeI CTNAG 2 cut(s) 157, 282
DpnI GATC 3 cut(s) 177, 220, 328
DpnII GATC 3 cut(s) 175, 218, 326
Eam1104I CTCTTC 3 cut(s) 92, 204, 339
EarI CTCTTC 3 cut(s) 92, 204, 339
EciI GGCGGA 1 cut(s) 345
Eco57I CTGAAG 1 cut(s) 186
Eco88I CYCGRG 1 cut(s) 262
FaiI YATR 3 cut(s) 107, 224, 297
FalI AAGNNNNNCTT 2 cut(s) 290, 322
Fnu4HI GCNGC 1 cut(s) 148
FokI GGATG 1 cut(s) 339
Fsp4HI GCNGC 1 cut(s) 148
FspBI CTAG 1 cut(s) 248
GluI GCNGC 1 cut(s) 148
HgaI GACGC 1 cut(s) 407
HincII GTYRAC 1 cut(s) 6
HindII GTYRAC 1 cut(s) 6
HinfI GANTC 4 cut(s) 65, 120, 161, 265
Hpy166II GTNNAC 1 cut(s) 6
Hpy188I TCNGA 7 cut(s) 14, 37, 119, 160, 166, 283, 343
Hpy188III TCNNGA 4 cut(s) 124, 248, 262, 324
Hpy8I GTNNAC 1 cut(s) 6
HpyCH4IV ACGT 1 cut(s) 438
HpyCH4V TGCA 3 cut(s) 41, 230, 352
HpyF3I CTNAG 2 cut(s) 157, 282
HpySE526I ACGT 1 cut(s) 438
Kzo9I GATC 3 cut(s) 175, 218, 326
LpnPI CCDG 3 cut(s) 86, 192, 337
LweI GCATC 2 cut(s) 28, 361
MaeI CTAG 1 cut(s) 248
MaeII ACGT 1 cut(s) 438
MaeIII GTNAC 2 cut(s) 241, 439
MalI GATC 3 cut(s) 177, 220, 328
MboI GATC 3 cut(s) 175, 218, 326
MboII GAAGA 8 cut(s) 79, 82, 179, 196, 221, 248, 356, 407
MflI RGATCY 2 cut(s) 218, 326
MhlI GDGCHC 1 cut(s) 408
MluCI AATT 2 cut(s) 137, 472
MlyI GAGTC 1 cut(s) 274
MnlI CCTC 3 cut(s) 83, 279, 373
MslI CAYNNNNRTG 1 cut(s) 437
NdeII GATC 3 cut(s) 175, 218, 326
NlaIV GGNNCC 1 cut(s) 220
NmuCI GTSAC 2 cut(s) 241, 439
NspV TTCGAA 1 cut(s) 207
OliI CACNNNNGTG 1 cut(s) 437
PaeR7I CTCGAG 1 cut(s) 262
PfeI GAWTC 3 cut(s) 65, 120, 161
PkrI GCNGC 1 cut(s) 149
PleI GAGTC 1 cut(s) 273
PpsI GAGTC 1 cut(s) 273
PspN4I GGNNCC 1 cut(s) 220
PstNI CAGNNNCTG 1 cut(s) 164
PsuI RGATCY 2 cut(s) 218, 326
RseI CAYNNNNRTG 1 cut(s) 437
SatI GCNGC 1 cut(s) 148
Sau3AI GATC 3 cut(s) 175, 218, 326
SchI GAGTC 1 cut(s) 274
SduI GDGCHC 1 cut(s) 408
SetI ASST 4 cut(s) 75, 384, 441, 464
SfaNI GCATC 2 cut(s) 28, 361
Sfr274I CTCGAG 1 cut(s) 262
SfuI TTCGAA 1 cut(s) 207
SlaI CTCGAG 1 cut(s) 262
SmiMI CAYNNNNRTG 1 cut(s) 437
SmlI CTYRAG 2 cut(s) 262, 269
SmoI CTYRAG 2 cut(s) 262, 269
Sse9I AATT 2 cut(s) 137, 472
SsiI CCGC 2 cut(s) 147, 356
SspI AATATT 1 cut(s) 21
SspMI CTAG 1 cut(s) 248
TaiI ACGT 1 cut(s) 441
TaqI TCGA 2 cut(s) 207, 263
TasI AATT 2 cut(s) 137, 472
TauI GCSGC 1 cut(s) 150
TfiI GAWTC 3 cut(s) 65, 120, 161
TscAI CASTG 1 cut(s) 67
TseFI GTSAC 2 cut(s) 241, 439
Tsp45I GTSAC 2 cut(s) 241, 439
TspDTI ATGAA 1 cut(s) 408
TspRI CASTG 1 cut(s) 67
XapI RAATTY 1 cut(s) 137
XbaI TCTAGA 1 cut(s) 247
XhoI CTCGAG 1 cut(s) 262
XspI CTAG 1 cut(s) 248
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.