FvH4_5g33900

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0005474
Physical Location & Seq
Reverse (-)
24615132 .. 24618311
3180 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g33900.t1

Sequence Viewer

Length: 528 bp
ATGAAGACCCTCCGAAGCAGAAAAACAGTGTCGATGGGCCGATACAATGGTCTTCTGGAATTTGCGCATGTTGTGATGATACGCAGAGCTGTACGCTACTTTCTGCCTTCTGACCTTGATTTATCACAAGGTTGTATAGGTTTATTTTGCCCATGCTATCTGTTTGGAAAGAATGCAGAGTTTTTGGGCTCTGGAACATTAATGGGCTCCTGTACGACTCATTTTATTCTGTGGGCACTTGTTAATAGCGTCTGCTGCTTATTAACTGATGGTGTTCTTTTGGGTATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGGGCCCTAAGAGCAAAATATAACCTCCAGGAAGCACCATGTGAAGATTTCATTACTCATTTTTTCTGCCATCTCTGTGCAATTTGCCAAGAGTACAGGGAGATCCGTGAAAAATCTGCTGAATCTGGCCCTGGTGATCTAAATGTAGCCATAGTTACAGCCCCAGCAGTCCAGACAATGGAACCTGCGGTTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

176

Amino Acids

19.38

Weight (kDa)

7.94

Isoelectric Point (pI)

42.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 40 - 143 7.2e-24 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 66
Acc36I ACCTGC 1 cut(s) 520
AccB7I CCANNNNNTGG 1 cut(s) 505
AccI GTMKAC 1 cut(s) 286
AciI CCGC 2 cut(s) 325, 515
AclWI GGATC 1 cut(s) 424
AcsI RAATTY 1 cut(s) 59
AdeI CACNNNGTG 1 cut(s) 368
AfaI GTAC 3 cut(s) 93, 214, 422
AfiI CCNNNNNNNGG 1 cut(s) 505
AjnI CCWGG 2 cut(s) 354, 457
AluBI AGCT 1 cut(s) 89
AluI AGCT 1 cut(s) 89
AlwI GGATC 1 cut(s) 424
AoxI GGCC 3 cut(s) 37, 330, 454
ApaI GGGCCC 1 cut(s) 334
ApeKI GCWGC 1 cut(s) 255
ApoI RAATTY 1 cut(s) 59
AseI ATTAAT 1 cut(s) 200
AspLEI GCGC 1 cut(s) 67
AspS9I GGNCC 4 cut(s) 37, 330, 331, 455
AsuC2I CCSGG 1 cut(s) 291
AsuHPI GGTGA 1 cut(s) 473
BaeGI GKGCMC 2 cut(s) 238, 334
BaeI ACNNNNGTAYC 2 cut(s) 34, 67
BanII GRGCYC 3 cut(s) 191, 209, 334
BbsI GAAGAC 2 cut(s) 11, 44
BbvI GCAGC 1 cut(s) 242
BccI CCATC 3 cut(s) 28, 263, 405
BciT130I CCWGG 2 cut(s) 356, 459
BcnI CCSGG 1 cut(s) 291
BfuAI ACCTGC 1 cut(s) 520
BisI GCNGC 1 cut(s) 256
BlsI GCNGC 1 cut(s) 257
Bme1390I CCNGG 3 cut(s) 291, 356, 459
BmgT120I GGNCC 4 cut(s) 37, 330, 331, 455
BmiI GGNNCC 3 cut(s) 208, 332, 510
BmrFI CCNGG 3 cut(s) 291, 356, 459
BmsI GCATC 1 cut(s) 284
BpiI GAAGAC 2 cut(s) 11, 44
BpmI CTGGAG 1 cut(s) 338
BpuMI CCSGG 1 cut(s) 291
BsaJI CCNNGG 1 cut(s) 457
Bsc4I CCNNNNNNNGG 1 cut(s) 505
BseBI CCWGG 2 cut(s) 356, 459
BseDI CCNNGG 1 cut(s) 457
BseGI GGATG 1 cut(s) 299
BseLI CCNNNNNNNGG 1 cut(s) 505
BseSI GKGCMC 2 cut(s) 238, 334
BseXI GCAGC 1 cut(s) 242
BseYI CCCAGC 1 cut(s) 490
BshFI GGCC 3 cut(s) 39, 332, 456
BsiSI CCGG 1 cut(s) 290
BslI CCNNNNNNNGG 1 cut(s) 505
BsmI GAATGC 1 cut(s) 178
BsnI GGCC 3 cut(s) 39, 332, 456
Bsp120I GGGCCC 1 cut(s) 330
Bsp1286I GDGCHC 4 cut(s) 191, 209, 238, 334
Bsp143I GATC 2 cut(s) 429, 463
BspACI CCGC 2 cut(s) 325, 515
BspANI GGCC 3 cut(s) 39, 332, 456
BspLI GGNNCC 3 cut(s) 208, 332, 510
BspMI ACCTGC 1 cut(s) 520
BspPI GGATC 1 cut(s) 424
BssECI CCNNGG 1 cut(s) 457
BssMI GATC 2 cut(s) 429, 463
BssNAI GTATAC 1 cut(s) 287
Bst1107I GTATAC 1 cut(s) 287
Bst2UI CCWGG 2 cut(s) 356, 459
Bst4CI ACNGT 1 cut(s) 28
BstAPI GCANNNNNTGC 1 cut(s) 311
BstDEI CTNAG 1 cut(s) 335
BstF5I GGATG 1 cut(s) 299
BstHHI GCGC 1 cut(s) 67
BstKTI GATC 2 cut(s) 432, 466
BstMBI GATC 2 cut(s) 429, 463
BstMWI GCNNNNNNNGC 3 cut(s) 255, 311, 338
BstNI CCWGG 2 cut(s) 356, 459
BstNSI RCATGY 2 cut(s) 71, 309
BstSCI CCNGG 3 cut(s) 289, 354, 457
BstSLI GKGCMC 2 cut(s) 238, 334
BstV1I GCAGC 1 cut(s) 242
BstV2I GAAGAC 2 cut(s) 11, 44
BstX2I RGATCY 1 cut(s) 429
BstYI RGATCY 1 cut(s) 429
BstZ17I GTATAC 1 cut(s) 287
BsuRI GGCC 3 cut(s) 39, 332, 456
BtsCI GGATG 1 cut(s) 299
BtsIMutI CAGTG 1 cut(s) 33
BveI ACCTGC 1 cut(s) 520
CfoI GCGC 1 cut(s) 67
Cfr13I GGNCC 4 cut(s) 37, 330, 331, 455
CseI GACGC 1 cut(s) 238
Csp6I GTAC 3 cut(s) 92, 213, 421
CviAII CATG 4 cut(s) 68, 153, 306, 366
CviJI RGCY 9 cut(s) 39, 89, 189, 207, 321, 332, 456, 476, 488
CviKI_1 RGCY 9 cut(s) 39, 89, 189, 207, 321, 332, 456, 476, 488
CviQI GTAC 3 cut(s) 92, 213, 421
DdeI CTNAG 1 cut(s) 335
DpnI GATC 2 cut(s) 431, 465
DpnII GATC 2 cut(s) 429, 463
DraIII CACNNNGTG 1 cut(s) 368
Eco24I GRGCYC 3 cut(s) 191, 209, 334
EcoO109I RGGNCCY 2 cut(s) 330, 331
EcoRII CCWGG 2 cut(s) 354, 457
EcoT38I GRGCYC 3 cut(s) 191, 209, 334
FaeI CATG 4 cut(s) 71, 156, 309, 369
FaiI YATR 9 cut(s) 69, 137, 154, 287, 307, 312, 348, 367, 479
FatI CATG 4 cut(s) 67, 152, 305, 365
FblI GTMKAC 1 cut(s) 286
Fnu4HI GCNGC 1 cut(s) 256
FokI GGATG 1 cut(s) 306
FriOI GRGCYC 3 cut(s) 191, 209, 334
Fsp4HI GCNGC 1 cut(s) 256
FspI TGCGCA 1 cut(s) 66
GlaI GCGC 1 cut(s) 66
GluI GCNGC 1 cut(s) 256
GsaI CCCAGC 1 cut(s) 494
GsuI CTGGAG 1 cut(s) 338
HaeIII GGCC 3 cut(s) 39, 332, 456
HapII CCGG 1 cut(s) 290
HgaI GACGC 1 cut(s) 238
HhaI GCGC 1 cut(s) 67
Hin1II CATG 4 cut(s) 71, 156, 309, 369
Hin6I GCGC 1 cut(s) 65
HinP1I GCGC 1 cut(s) 65
HincII GTYRAC 1 cut(s) 520
HindII GTYRAC 1 cut(s) 520
HinfI GANTC 2 cut(s) 217, 449
HpaII CCGG 1 cut(s) 290
HphI GGTGA 1 cut(s) 473
Hpy166II GTNNAC 2 cut(s) 287, 520
Hpy188I TCNGA 2 cut(s) 14, 112
Hpy188III TCNNGA 3 cut(s) 56, 192, 499
Hpy8I GTNNAC 2 cut(s) 287, 520
HpyAV CCTTC 1 cut(s) 117
HpyCH4III ACNGT 1 cut(s) 28
HpyCH4V TGCA 3 cut(s) 176, 305, 407
HpyF10VI GCNNNNNNNGC 3 cut(s) 255, 311, 338
HpyF3I CTNAG 1 cut(s) 335
Hsp92II CATG 4 cut(s) 71, 156, 309, 369
HspAI GCGC 1 cut(s) 65
Kzo9I GATC 2 cut(s) 429, 463
LmnI GCTCC 1 cut(s) 212
Lsp1109I GCAGC 1 cut(s) 242
LweI GCATC 1 cut(s) 284
MaeIII GTNAC 1 cut(s) 481
MalI GATC 2 cut(s) 431, 465
MboI GATC 2 cut(s) 429, 463
MboII GAAGA 3 cut(s) 16, 44, 383
MflI RGATCY 1 cut(s) 429
MhlI GDGCHC 4 cut(s) 191, 209, 238, 334
MluCI AATT 2 cut(s) 59, 408
MlyI GAGTC 1 cut(s) 211
MnlI CCTC 2 cut(s) 20, 362
MseI TTAA 3 cut(s) 200, 243, 263
MslI CAYNNNNRTG 1 cut(s) 402
MspI CCGG 1 cut(s) 290
MspR9I CCNGG 3 cut(s) 291, 356, 459
Mva1269I GAATGC 1 cut(s) 178
MvaI CCWGG 2 cut(s) 356, 459
MwoI GCNNNNNNNGC 3 cut(s) 255, 311, 338
NciI CCSGG 1 cut(s) 291
NdeII GATC 2 cut(s) 429, 463
NlaIII CATG 4 cut(s) 71, 156, 309, 369
NlaIV GGNNCC 3 cut(s) 208, 332, 510
NsbI TGCGCA 1 cut(s) 66
NspI RCATGY 2 cut(s) 71, 309
PctI GAATGC 1 cut(s) 178
PfeI GAWTC 1 cut(s) 449
PflMI CCANNNNNTGG 1 cut(s) 505
PfoI TCCNGGA 1 cut(s) 354
PkrI GCNGC 1 cut(s) 257
PleI GAGTC 1 cut(s) 211
PpsI GAGTC 1 cut(s) 211
PshBI ATTAAT 1 cut(s) 200
Psp6I CCWGG 2 cut(s) 354, 457
PspFI CCCAGC 1 cut(s) 490
PspGI CCWGG 2 cut(s) 354, 457
PspN4I GGNNCC 3 cut(s) 208, 332, 510
PspOMI GGGCCC 1 cut(s) 330
PspPI GGNCC 4 cut(s) 37, 330, 331, 455
PsuI RGATCY 1 cut(s) 429
RsaI GTAC 3 cut(s) 93, 214, 422
RsaNI GTAC 3 cut(s) 92, 213, 421
RseI CAYNNNNRTG 1 cut(s) 402
SaqAI TTAA 3 cut(s) 200, 243, 263
SatI GCNGC 1 cut(s) 256
Sau3AI GATC 2 cut(s) 429, 463
Sau96I GGNCC 4 cut(s) 37, 330, 331, 455
SchI GAGTC 1 cut(s) 211
ScrFI CCNGG 3 cut(s) 291, 356, 459
SduI GDGCHC 4 cut(s) 191, 209, 238, 334
SetI ASST 6 cut(s) 91, 117, 133, 142, 354, 514
SfaNI GCATC 1 cut(s) 284
SmiMI CAYNNNNRTG 1 cut(s) 402
Sse9I AATT 2 cut(s) 59, 408
SsiI CCGC 2 cut(s) 325, 515
StyD4I CCNGG 3 cut(s) 289, 354, 457
TaaI ACNGT 1 cut(s) 28
TaqI TCGA 1 cut(s) 32
TasI AATT 2 cut(s) 59, 408
TatI WGTACW 1 cut(s) 420
TfiI GAWTC 1 cut(s) 449
Tru1I TTAA 3 cut(s) 200, 243, 263
Tru9I TTAA 3 cut(s) 200, 243, 263
TscAI CASTG 1 cut(s) 33
TseI GCWGC 1 cut(s) 255
TspDTI ATGAA 2 cut(s) 17, 367
TspGWI ACGGA 1 cut(s) 422
TspRI CASTG 1 cut(s) 33
Van91I CCANNNNNTGG 1 cut(s) 505
VspI ATTAAT 1 cut(s) 200
XapI RAATTY 1 cut(s) 59
XceI RCATGY 2 cut(s) 71, 309
XmiI GTMKAC 1 cut(s) 286
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.