Rw3G027960

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0005474
Physical Location & Seq
Reverse (-)
40701891 .. 40705101
3211 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G027960.1

Sequence Viewer

Length: 591 bp
ATGCACCTCCGTCTTATATTCCCTTGGGCCAATCAGATTCGGGATCACCTGTTGTTTTACGCAATGAAGACCCTCCCAAGCAGCAAAATAATGTCGATCGGCCGATACAATGGTCTTCTGGAATTTGCGCATGTTGTGATGATACGCAGAGCTGTACGCTACTTTCTGCCTTCTGACCTTGATTTATCACAAGGTTGTATAGGTTTATTTTGTCCTTGCTATCTGTTCGGAAAGAATGCAGAGTTTCTGGGCTCTGGGACATTAATGGGCTCCTGCACGACTCATTTTATTCTGTGGGTACTTGTTAATACCGTCTGCTGCTTATTAACTGATGGCCTTATTTTGGGTATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGGGCACTAAGATCAAAATATAATCTCCAGGAAGCACCGTGTGGAGACTTTATTACTCATTTTTTCTGCCATCTGTGCGCAATTTGTCAAGAGTACAGGGAGATCCGTGAAAAGTCTTCTGAATCTGGCCCTGGTGATCTGAATCTAGCCATAGTTACAGCCCCAGCAGTCCAGACAATGGAACCAGGGCTTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

196

Amino Acids

21.95

Weight (kDa)

7.94

Isoelectric Point (pI)

50.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 61 - 164 7e-24 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 129, 469
AccB7I CCANNNNNTGG 1 cut(s) 568
AccI GTMKAC 1 cut(s) 349
AciI CCGC 1 cut(s) 388
AclWI GGATC 2 cut(s) 51, 487
AcoI YGGCCR 1 cut(s) 100
AcsI RAATTY 1 cut(s) 122
AdeI CACNNNGTG 1 cut(s) 431
AfaI GTAC 3 cut(s) 156, 300, 485
AfiI CCNNNNNNNGG 2 cut(s) 343, 568
AjnI CCWGG 3 cut(s) 417, 520, 574
AluBI AGCT 1 cut(s) 152
AluI AGCT 1 cut(s) 152
Alw26I GTCTC 1 cut(s) 429
AlwI GGATC 2 cut(s) 51, 487
AoxI GGCC 4 cut(s) 27, 100, 334, 517
ApeKI GCWGC 2 cut(s) 81, 318
ApoI RAATTY 1 cut(s) 122
AseI ATTAAT 1 cut(s) 263
AspLEI GCGC 2 cut(s) 130, 470
AspS9I GGNCC 2 cut(s) 27, 518
AsuC2I CCSGG 1 cut(s) 354
AsuHPI GGTGA 2 cut(s) 38, 536
BaeGI GKGCMC 1 cut(s) 397
BaeI ACNNNNGTAYC 2 cut(s) 97, 130
BanII GRGCYC 2 cut(s) 254, 272
BbsI GAAGAC 3 cut(s) 74, 107, 498
BbvI GCAGC 2 cut(s) 93, 305
BccI CCATC 2 cut(s) 326, 468
BciT130I CCWGG 3 cut(s) 419, 522, 576
BcnI CCSGG 1 cut(s) 354
BcoDI GTCTC 1 cut(s) 429
BfaI CTAG 1 cut(s) 536
BisI GCNGC 2 cut(s) 82, 319
BlsI GCNGC 2 cut(s) 83, 320
Bme1390I CCNGG 4 cut(s) 354, 419, 522, 576
BmgT120I GGNCC 2 cut(s) 27, 518
BmiI GGNNCC 2 cut(s) 271, 573
BmrFI CCNGG 4 cut(s) 354, 419, 522, 576
BmsI GCATC 1 cut(s) 347
BpiI GAAGAC 3 cut(s) 74, 107, 498
BpmI CTGGAG 1 cut(s) 401
BpuMI CCSGG 1 cut(s) 354
BsaBI GATNNNNATC 1 cut(s) 531
BsaJI CCNNGG 3 cut(s) 23, 520, 575
Bsc4I CCNNNNNNNGG 2 cut(s) 343, 568
Bse3DI GCAATG 1 cut(s) 69
Bse8I GATNNNNATC 1 cut(s) 531
BseBI CCWGG 3 cut(s) 419, 522, 576
BseDI CCNNGG 3 cut(s) 23, 520, 575
BseGI GGATG 1 cut(s) 362
BseJI GATNNNNATC 1 cut(s) 531
BseLI CCNNNNNNNGG 2 cut(s) 343, 568
BseMI GCAATG 1 cut(s) 69
BseSI GKGCMC 1 cut(s) 397
BseX3I CGGCCG 1 cut(s) 100
BseXI GCAGC 2 cut(s) 93, 305
BseYI CCCAGC 1 cut(s) 553
BsgI GTGCAG 1 cut(s) 259
Bsh1285I CGRYCG 2 cut(s) 99, 103
BshFI GGCC 4 cut(s) 29, 102, 336, 519
BsiEI CGRYCG 2 cut(s) 99, 103
BsiSI CCGG 1 cut(s) 353
BslFI GGGAC 1 cut(s) 271
BslI CCNNNNNNNGG 2 cut(s) 343, 568
BsmAI GTCTC 1 cut(s) 429
BsmFI GGGAC 1 cut(s) 271
BsmI GAATGC 1 cut(s) 241
BsnI GGCC 4 cut(s) 29, 102, 336, 519
Bsp1286I GDGCHC 3 cut(s) 254, 272, 397
Bsp143I GATC 5 cut(s) 43, 96, 401, 492, 526
BspACI CCGC 1 cut(s) 388
BspANI GGCC 4 cut(s) 29, 102, 336, 519
BspLI GGNNCC 2 cut(s) 271, 573
BspPI GGATC 2 cut(s) 51, 487
BsrDI GCAATG 1 cut(s) 69
BssECI CCNNGG 3 cut(s) 23, 520, 575
BssMI GATC 5 cut(s) 43, 96, 401, 492, 526
BssNAI GTATAC 1 cut(s) 350
BssT1I CCWWGG 1 cut(s) 23
Bst1107I GTATAC 1 cut(s) 350
Bst2UI CCWGG 3 cut(s) 419, 522, 576
Bst4CI ACNGT 2 cut(s) 313, 429
BstAPI GCANNNNNTGC 1 cut(s) 374
BstDEI CTNAG 1 cut(s) 398
BstF5I GGATG 1 cut(s) 362
BstHHI GCGC 2 cut(s) 130, 470
BstKTI GATC 5 cut(s) 46, 99, 404, 495, 529
BstMAI GTCTC 1 cut(s) 429
BstMBI GATC 5 cut(s) 43, 96, 401, 492, 526
BstMCI CGRYCG 2 cut(s) 99, 103
BstMWI GCNNNNNNNGC 2 cut(s) 374, 465
BstNI CCWGG 3 cut(s) 419, 522, 576
BstNSI RCATGY 2 cut(s) 134, 372
BstSCI CCNGG 4 cut(s) 352, 417, 520, 574
BstSLI GKGCMC 1 cut(s) 397
BstV1I GCAGC 2 cut(s) 93, 305
BstV2I GAAGAC 3 cut(s) 74, 107, 498
BstX2I RGATCY 1 cut(s) 492
BstYI RGATCY 1 cut(s) 492
BstZ17I GTATAC 1 cut(s) 350
BstZI CGGCCG 1 cut(s) 100
BsuRI GGCC 4 cut(s) 29, 102, 336, 519
BtsCI GGATG 1 cut(s) 362
CfoI GCGC 2 cut(s) 130, 470
Cfr13I GGNCC 2 cut(s) 27, 518
Csp6I GTAC 3 cut(s) 155, 299, 484
CviAII CATG 2 cut(s) 131, 369
CviQI GTAC 3 cut(s) 155, 299, 484
DdeI CTNAG 1 cut(s) 398
DpnI GATC 5 cut(s) 45, 98, 403, 494, 528
DpnII GATC 5 cut(s) 43, 96, 401, 492, 526
DraIII CACNNNGTG 1 cut(s) 431
EaeI YGGCCR 1 cut(s) 100
EagI CGGCCG 1 cut(s) 100
EclXI CGGCCG 1 cut(s) 100
Eco130I CCWWGG 1 cut(s) 23
Eco24I GRGCYC 2 cut(s) 254, 272
Eco52I CGGCCG 1 cut(s) 100
EcoRII CCWGG 3 cut(s) 417, 520, 574
EcoT14I CCWWGG 1 cut(s) 23
EcoT38I GRGCYC 2 cut(s) 254, 272
ErhI CCWWGG 1 cut(s) 23
FaeI CATG 2 cut(s) 134, 372
FaiI YATR 8 cut(s) 17, 132, 200, 350, 370, 375, 411, 542
FaqI GGGAC 1 cut(s) 271
FatI CATG 2 cut(s) 130, 368
FblI GTMKAC 1 cut(s) 349
Fnu4HI GCNGC 2 cut(s) 82, 319
FokI GGATG 1 cut(s) 369
FriOI GRGCYC 2 cut(s) 254, 272
Fsp4HI GCNGC 2 cut(s) 82, 319
FspBI CTAG 1 cut(s) 536
FspI TGCGCA 2 cut(s) 129, 469
GlaI GCGC 2 cut(s) 129, 469
GluI GCNGC 2 cut(s) 82, 319
GsaI CCCAGC 1 cut(s) 557
GsuI CTGGAG 1 cut(s) 401
HaeIII GGCC 4 cut(s) 29, 102, 336, 519
HapII CCGG 1 cut(s) 353
HhaI GCGC 2 cut(s) 130, 470
Hin1II CATG 2 cut(s) 134, 372
Hin6I GCGC 2 cut(s) 128, 468
HinP1I GCGC 2 cut(s) 128, 468
HinfI GANTC 4 cut(s) 37, 280, 512, 532
HpaII CCGG 1 cut(s) 353
HphI GGTGA 2 cut(s) 38, 536
Hpy166II GTNNAC 1 cut(s) 350
Hpy188I TCNGA 5 cut(s) 36, 175, 230, 511, 531
Hpy188III TCNNGA 4 cut(s) 41, 119, 479, 562
Hpy8I GTNNAC 1 cut(s) 350
HpyAV CCTTC 1 cut(s) 180
HpyCH4III ACNGT 2 cut(s) 313, 429
HpyCH4V TGCA 4 cut(s) 4, 239, 276, 368
HpyF10VI GCNNNNNNNGC 2 cut(s) 374, 465
HpyF3I CTNAG 1 cut(s) 398
Hsp92II CATG 2 cut(s) 134, 372
HspAI GCGC 2 cut(s) 128, 468
Kzo9I GATC 5 cut(s) 43, 96, 401, 492, 526
LmnI GCTCC 1 cut(s) 275
Lsp1109I GCAGC 2 cut(s) 93, 305
LweI GCATC 1 cut(s) 347
MaeI CTAG 1 cut(s) 536
MaeIII GTNAC 1 cut(s) 544
MalI GATC 5 cut(s) 45, 98, 403, 494, 528
MboI GATC 5 cut(s) 43, 96, 401, 492, 526
MboII GAAGA 3 cut(s) 79, 107, 498
MflI RGATCY 1 cut(s) 492
MhlI GDGCHC 3 cut(s) 254, 272, 397
MluCI AATT 2 cut(s) 122, 471
MlyI GAGTC 1 cut(s) 274
MnlI CCTC 2 cut(s) 17, 83
MseI TTAA 3 cut(s) 263, 306, 326
MspI CCGG 1 cut(s) 353
MspR9I CCNGG 4 cut(s) 354, 419, 522, 576
Mva1269I GAATGC 1 cut(s) 241
MvaI CCWGG 3 cut(s) 419, 522, 576
MwoI GCNNNNNNNGC 2 cut(s) 374, 465
NciI CCSGG 1 cut(s) 354
NdeII GATC 5 cut(s) 43, 96, 401, 492, 526
NlaIII CATG 2 cut(s) 134, 372
NlaIV GGNNCC 2 cut(s) 271, 573
NsbI TGCGCA 2 cut(s) 129, 469
NspI RCATGY 2 cut(s) 134, 372
PctI GAATGC 1 cut(s) 241
PfeI GAWTC 3 cut(s) 37, 512, 532
PflMI CCANNNNNTGG 1 cut(s) 568
PfoI TCCNGGA 1 cut(s) 417
PkrI GCNGC 2 cut(s) 83, 320
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 1 cut(s) 274
PpsI GAGTC 1 cut(s) 274
PshBI ATTAAT 1 cut(s) 263
Psp6I CCWGG 3 cut(s) 417, 520, 574
PspFI CCCAGC 1 cut(s) 553
PspGI CCWGG 3 cut(s) 417, 520, 574
PspN4I GGNNCC 2 cut(s) 271, 573
PspPI GGNCC 2 cut(s) 27, 518
PsuI RGATCY 1 cut(s) 492
PvuI CGATCG 1 cut(s) 99
RsaI GTAC 3 cut(s) 156, 300, 485
RsaNI GTAC 3 cut(s) 155, 299, 484
SaqAI TTAA 3 cut(s) 263, 306, 326
SatI GCNGC 2 cut(s) 82, 319
Sau3AI GATC 5 cut(s) 43, 96, 401, 492, 526
Sau96I GGNCC 2 cut(s) 27, 518
SchI GAGTC 1 cut(s) 274
ScrFI CCNGG 4 cut(s) 354, 419, 522, 576
SduI GDGCHC 3 cut(s) 254, 272, 397
SetI ASST 6 cut(s) 9, 51, 154, 180, 196, 205
SfaNI GCATC 1 cut(s) 347
Sse9I AATT 2 cut(s) 122, 471
SsiI CCGC 1 cut(s) 388
SspMI CTAG 1 cut(s) 536
StyD4I CCNGG 4 cut(s) 352, 417, 520, 574
StyI CCWWGG 1 cut(s) 23
TaaI ACNGT 2 cut(s) 313, 429
TaqI TCGA 1 cut(s) 95
TasI AATT 2 cut(s) 122, 471
TatI WGTACW 1 cut(s) 483
TfiI GAWTC 3 cut(s) 37, 512, 532
Tru1I TTAA 3 cut(s) 263, 306, 326
Tru9I TTAA 3 cut(s) 263, 306, 326
TseI GCWGC 2 cut(s) 81, 318
TspDTI ATGAA 1 cut(s) 80
TspGWI ACGGA 1 cut(s) 485
Van91I CCANNNNNTGG 1 cut(s) 568
VspI ATTAAT 1 cut(s) 263
XapI RAATTY 1 cut(s) 122
XceI RCATGY 2 cut(s) 134, 372
XmiI GTMKAC 1 cut(s) 349
XspI CTAG 1 cut(s) 536
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.