Rorug03G0271500

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005474
Physical Location & Seq
Reverse (-)
26779547 .. 26783073
3527 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0271500.1

Sequence Viewer

Length: 1425 bp
ATGATGATGCTGCGAAGGCCGAAACCAAGGTGCCTTCCAAGTTCTGGTTCGAAGAATGAAGCTGCAGCAATTAGCAACACAGGTTTAGGTGGCATGGATCCAGAGATATTCGCAGCAGCAGCCGAAGGAAATATTAAAGCCCTTTCAACACATGGGGAGCTTCTGCATGAAATACTCACTCCAACCAAAAACACTGTCCTACATATTTATGTAGCACATGCAAGCTACCCAACCTTAACAAAACTCGAAAAGTTTGACAGCTCCAGATATGGCCTCCCTGGTCTACTTGCTGATTTGCTCATTAAAAAGCTAATGCCATGCATATGGGACGCAGAAAACACAGTTAGGCCAACTACCTTAACTGCATCCCAAGTACAGAGGTCAAAGGAGATTGTCACTCAGTTGCTTGAAGTGTGTCCAAAGCTACTTTTGCAGCCCAACAATAATGGCGACATTGCACTACACATTGCGGCCAGATATGGGCTTGATGGTGTAGTTGAAGTTCTTATTGGGGCAGCAAAAGCTCGTCATGGCGATGTTGAGAAACAGGTTGAAGAAGCTTGGCAAATGTTGAGGACACCCAACAAGGAGGGAGACACAGCCCTACACGAGGCAGCGCGCTTTAATCACCTCGGTGTGGTGAAGAAATTGTGCAGAGAATACCCTGATTTCTCCTACTCGGCTAATGTGGCTAGAAGGACTCCAATTTACTTGGCTGCTGAAAGGGGATATCGAGACTTGGTTTCTGAAATACTGGATTCTTGTACTTATCCAGGCTACCAAGGCCCTAGTGGTAAAACAGCTTTACATGCTGCAGTGATCTTCAGTGATATAGAAATGACGCAAAAATTGCTAGACAAGGAGAAGACCCTGGCACAAGCAAGAGATGAACAAGGATGGACTCCACTTCACTTTGCTGCACTCATCGGTAACAGATGGATTGTGAGCCGGCTACTAGATTGTGATAAATCAATTGCCTATATCGTTGACAAAGATGACAAGAAGACAGCTCTTCACATTGCAGCTTCCAAAGGCCATGAAGGCGTACTGACAGAGCTTATATATCGTTGCCCTGATTGTTGTGAAGTAGTCGACCGACGCCAGCGGAATATCCTTCATTATGCCATTGAGAACAGGAAATTTGAAATAGCGAAGGTTGTTCAAAACCATCCATGGATGAGCAACATCCTTCTAAATGGTAAAGATGTTGCCGGAAACACACCCCTCCATCGACTAGTCAACATACGCCTCCAAACAGCTACTGAATCTTTATTTCGCTATGGAAAAGCTCCTACCCGGTTGAGTCGGTTCATTCATGATGCTAGAGTGGATAAGAGGGCTTTGAACAAGGAAGACCTAAATGCTATTGACATCATTCTTGATGATGACCATACTTTATCAGAAGTGGAGAAAGTTGGTCGTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

474

Amino Acids

52.93

Weight (kDa)

8.25

Isoelectric Point (pI)

38.93

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ank_2 PF12796 129 - 221 3.1e-09 Ankyrin repeats (3 copies)
Ank_2 PF12796 194 - 253 5.9e-07 Ankyrin repeats (3 copies)
Ank_2 PF12796 237 - 322 1.3e-12 Ankyrin repeats (3 copies)
Ank_4 PF13637 269 - 316 2.2e-07 Ankyrin repeats (many copies)
Ank_2 PF12796 330 - 386 1.1e-07 Ankyrin repeats (3 copies)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 30
AccB7I CCANNNNNTGG 1 cut(s) 44
AccI GTMKAC 2 cut(s) 283, 1092
AccII CGCG 1 cut(s) 619
AciI CCGC 2 cut(s) 470, 1105
AclWI GGATC 2 cut(s) 92, 105
AcoI YGGCCR 1 cut(s) 471
AcsI RAATTY 1 cut(s) 1139
AcuI CTGAAG 1 cut(s) 808
AcyI GRCGYC 1 cut(s) 1099
AfaI GTAC 3 cut(s) 375, 766, 1047
AfiI CCNNNNNNNGG 4 cut(s) 44, 480, 610, 637
AgsI TTSAA 7 cut(s) 147, 410, 500, 554, 1145, 1163, 1345
AhlI ACTAGT 1 cut(s) 1234
AjnI CCWGG 3 cut(s) 277, 772, 870
AjuI GAANNNNNNNTTGG 4 cut(s) 31, 63, 492, 524
AleI CACNNNNGTG 1 cut(s) 633
Alw26I GTCTC 2 cut(s) 588, 729
AlwI GGATC 2 cut(s) 92, 105
AlwNI CAGNNNCTG 1 cut(s) 1262
AoxI GGCC 6 cut(s) 17, 271, 347, 471, 784, 1033
ApoI RAATTY 1 cut(s) 1139
ArsI GACNNNNNNTTYG 2 cut(s) 180, 212
AspLEI GCGC 2 cut(s) 619, 621
AspS9I GGNCC 1 cut(s) 785
AsuC2I CCSGG 1 cut(s) 1297
AsuHPI GGTGA 2 cut(s) 620, 652
AsuII TTCGAA 1 cut(s) 50
BamHI GGATCC 1 cut(s) 97
BanI GGYRCC 1 cut(s) 30
BauI CACGAG 1 cut(s) 608
BbsI GAAGAC 3 cut(s) 872, 1010, 1359
BccI CCATC 5 cut(s) 482, 891, 930, 1176, 1236
BciT130I CCWGG 3 cut(s) 279, 774, 872
BcnI CCSGG 1 cut(s) 1297
BcoDI GTCTC 2 cut(s) 588, 729
BcuI ACTAGT 1 cut(s) 1234
BfaI CTAG 6 cut(s) 693, 789, 854, 956, 1235, 1323
BfmI CTRYAG 2 cut(s) 63, 813
BglI GCCNNNNNGGC 1 cut(s) 1041
Bme1390I CCNGG 4 cut(s) 279, 774, 872, 1297
BmgT120I GGNCC 1 cut(s) 785
BmiI GGNNCC 2 cut(s) 32, 99
BmrFI CCNGG 4 cut(s) 279, 774, 872, 1297
BmsI GCATC 2 cut(s) 374, 1309
BpiI GAAGAC 3 cut(s) 872, 1010, 1359
BpmI CTGGAG 1 cut(s) 247
Bpu14I TTCGAA 1 cut(s) 50
BpuMI CCSGG 1 cut(s) 1297
BsaHI GRCGYC 1 cut(s) 1099
BsaJI CCNNGG 6 cut(s) 26, 277, 631, 781, 870, 1172
Bsc4I CCNNNNNNNGG 4 cut(s) 44, 480, 610, 637
Bse118I RCCGGY 1 cut(s) 948
Bse1I ACTGG 1 cut(s) 759
Bse3DI GCAATG 3 cut(s) 453, 465, 1017
BseBI CCWGG 3 cut(s) 279, 774, 872
BseDI CCNNGG 6 cut(s) 26, 277, 631, 781, 870, 1172
BseGI GGATG 5 cut(s) 365, 902, 1168, 1182, 1185
BseLI CCNNNNNNNGG 4 cut(s) 44, 480, 610, 637
BseMI GCAATG 3 cut(s) 453, 465, 1017
BseMII CTCAG 1 cut(s) 413
BseNI ACTGG 1 cut(s) 759
BsePI GCGCGC 1 cut(s) 617
BsgI GTGCAG 2 cut(s) 673, 903
Bsh1236I CGCG 1 cut(s) 619
Bsh1285I CGRYCG 1 cut(s) 1096
BshFI GGCC 6 cut(s) 19, 273, 349, 473, 786, 1035
BshNI GGYRCC 1 cut(s) 30
BsiEI CGRYCG 1 cut(s) 1096
BsiSI CCGG 3 cut(s) 949, 1212, 1297
BslFI GGGAC 1 cut(s) 341
BslI CCNNNNNNNGG 4 cut(s) 44, 480, 610, 637
BsmAI GTCTC 2 cut(s) 588, 729
BsmFI GGGAC 1 cut(s) 341
BsnI GGCC 6 cut(s) 19, 273, 349, 473, 786, 1035
Bsp119I TTCGAA 1 cut(s) 50
Bsp143I GATC 2 cut(s) 97, 819
Bsp19I CCATGG 1 cut(s) 1172
BspACI CCGC 2 cut(s) 470, 1105
BspANI GGCC 6 cut(s) 19, 273, 349, 473, 786, 1035
BspCNI CTCAG 1 cut(s) 412
BspFNI CGCG 1 cut(s) 619
BspHI TCATGA 1 cut(s) 1315
BspLI GGNNCC 2 cut(s) 32, 99
BspMAI CTGCAG 2 cut(s) 67, 817
BspPI GGATC 2 cut(s) 92, 105
BspQI GCTCTTC 1 cut(s) 1017
BspT104I TTCGAA 1 cut(s) 50
BspT107I GGYRCC 1 cut(s) 30
BsrDI GCAATG 3 cut(s) 453, 465, 1017
BsrFI RCCGGY 1 cut(s) 948
BsrI ACTGG 1 cut(s) 759
BssAI RCCGGY 1 cut(s) 948
BssECI CCNNGG 6 cut(s) 26, 277, 631, 781, 870, 1172
BssHII GCGCGC 1 cut(s) 617
BssMI GATC 2 cut(s) 97, 819
BssNI GRCGYC 1 cut(s) 1099
BssSI CACGAG 1 cut(s) 608
BssT1I CCWWGG 3 cut(s) 26, 781, 1172
Bst2BI CACGAG 1 cut(s) 608
Bst2UI CCWGG 3 cut(s) 279, 774, 872
Bst4CI ACNGT 2 cut(s) 196, 343
Bst6I CTCTTC 1 cut(s) 1017
BstACI GRCGYC 1 cut(s) 1099
BstAPI GCANNNNNTGC 1 cut(s) 850
BstBI TTCGAA 1 cut(s) 50
BstC8I GCNNGC 4 cut(s) 223, 619, 950, 1103
BstDEI CTNAG 1 cut(s) 399
BstDSI CCRYGG 1 cut(s) 1172
BstENI CCTNNNNNAGG 1 cut(s) 608
BstF5I GGATG 5 cut(s) 365, 902, 1168, 1182, 1185
BstFNI CGCG 1 cut(s) 619
BstHHI GCGC 2 cut(s) 619, 621
BstKTI GATC 2 cut(s) 100, 822
BstMAI GTCTC 2 cut(s) 588, 729
BstMBI GATC 2 cut(s) 97, 819
BstMCI CGRYCG 1 cut(s) 1096
BstMWI GCNNNNNNNGC 9 cut(s) 16, 119, 430, 521, 689, 783, 809, 850, 1041
BstNI CCWGG 3 cut(s) 279, 774, 872
BstNSI RCATGY 2 cut(s) 221, 812
BstSCI CCNGG 4 cut(s) 277, 772, 870, 1295
BstSFI CTRYAG 2 cut(s) 63, 813
BstUI CGCG 1 cut(s) 619
BstV2I GAAGAC 3 cut(s) 872, 1010, 1359
BstX2I RGATCY 1 cut(s) 97
BstXI CCANNNNNNTGG 1 cut(s) 324
BstYI RGATCY 1 cut(s) 97
BsuRI GGCC 6 cut(s) 19, 273, 349, 473, 786, 1035
BtgI CCRYGG 1 cut(s) 1172
BtgZI GCGATG 1 cut(s) 549
BtsCI GGATG 5 cut(s) 365, 902, 1168, 1182, 1185
BtsI GCAGTG 1 cut(s) 822
BtsIMutI CAGTG 3 cut(s) 192, 822, 832
Cac8I GCNNGC 4 cut(s) 223, 619, 950, 1103
CaiI CAGNNNCTG 1 cut(s) 1262
CciI TCATGA 1 cut(s) 1315
CfoI GCGC 2 cut(s) 619, 621
Cfr10I RCCGGY 1 cut(s) 948
Cfr13I GGNCC 1 cut(s) 785
CseI GACGC 3 cut(s) 338, 850, 1107
Csp6I GTAC 3 cut(s) 374, 765, 1046
CviQI GTAC 3 cut(s) 374, 765, 1046
DdeI CTNAG 1 cut(s) 399
DpnI GATC 2 cut(s) 99, 821
DpnII GATC 2 cut(s) 97, 819
EaeI YGGCCR 1 cut(s) 471
Eam1104I CTCTTC 1 cut(s) 1017
EarI CTCTTC 1 cut(s) 1017
Eco130I CCWWGG 3 cut(s) 26, 781, 1172
Eco32I GATATC 1 cut(s) 731
Eco57I CTGAAG 1 cut(s) 808
EcoNI CCTNNNNNAGG 1 cut(s) 608
EcoO109I RGGNCCY 1 cut(s) 785
EcoRII CCWGG 3 cut(s) 277, 772, 870
EcoRV GATATC 1 cut(s) 731
EcoT14I CCWWGG 3 cut(s) 26, 781, 1172
EcoT22I ATGCAT 1 cut(s) 323
ErhI CCWWGG 3 cut(s) 26, 781, 1172
FaqI GGGAC 1 cut(s) 341
FauNDI CATATG 1 cut(s) 323
FblI GTMKAC 2 cut(s) 283, 1092
FokI GGATG 5 cut(s) 352, 909, 1155, 1172, 1189
FspBI CTAG 6 cut(s) 693, 789, 854, 956, 1235, 1323
GlaI GCGC 2 cut(s) 618, 620
GsuI CTGGAG 1 cut(s) 247
HaeIII GGCC 6 cut(s) 19, 273, 349, 473, 786, 1035
HapII CCGG 3 cut(s) 949, 1212, 1297
HgaI GACGC 3 cut(s) 338, 850, 1107
HhaI GCGC 2 cut(s) 619, 621
Hin1I GRCGYC 1 cut(s) 1099
Hin6I GCGC 2 cut(s) 617, 619
HinP1I GCGC 2 cut(s) 617, 619
HincII GTYRAC 3 cut(s) 988, 1093, 1240
HindII GTYRAC 3 cut(s) 988, 1093, 1240
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 5 cut(s) 700, 758, 901, 1265, 1303
HpaII CCGG 3 cut(s) 949, 1212, 1297
HphI GGTGA 2 cut(s) 620, 652
Hpy166II GTNNAC 4 cut(s) 284, 988, 1093, 1240
Hpy188I TCNGA 2 cut(s) 748, 1402
Hpy188III TCNNGA 5 cut(s) 101, 264, 734, 1316, 1379
Hpy8I GTNNAC 4 cut(s) 284, 988, 1093, 1240
Hpy99I CGWCG 1 cut(s) 1101
HpyAV CCTTC 8 cut(s) 9, 44, 119, 690, 1034, 1124, 1147, 1199
HpyCH4III ACNGT 2 cut(s) 196, 343
HpyF10VI GCNNNNNNNGC 9 cut(s) 16, 119, 430, 521, 689, 783, 809, 850, 1041
HpyF3I CTNAG 1 cut(s) 399
Hsp92I GRCGYC 1 cut(s) 1099
HspAI GCGC 2 cut(s) 617, 619
KroI GCCGGC 1 cut(s) 948
KroNI GCCGGC 1 cut(s) 950
Kzo9I GATC 2 cut(s) 97, 819
LguI GCTCTTC 1 cut(s) 1017
LmnI GCTCC 3 cut(s) 157, 266, 1294
LweI GCATC 2 cut(s) 374, 1309
MaeI CTAG 6 cut(s) 693, 789, 854, 956, 1235, 1323
MaeIII GTNAC 2 cut(s) 394, 929
MalI GATC 2 cut(s) 99, 821
MboI GATC 2 cut(s) 97, 819
MboII GAAGA 8 cut(s) 64, 566, 655, 814, 877, 1004, 1015, 1364
MfeI CAATTG 1 cut(s) 972
MflI RGATCY 1 cut(s) 97
MluCI AATT 6 cut(s) 69, 647, 705, 848, 972, 1139
MlyI GAGTC 3 cut(s) 694, 895, 1312
MmeI TCCRAC 1 cut(s) 206
MnlI CCTC 9 cut(s) 284, 372, 567, 583, 604, 641, 1235, 1259, 1329
Mph1103I ATGCAT 1 cut(s) 323
MroNI GCCGGC 1 cut(s) 948
MseI TTAA 6 cut(s) 135, 236, 303, 359, 624, 1423
MslI CAYNNNNRTG 4 cut(s) 207, 322, 534, 633
MspA1I CMGCKG 1 cut(s) 1105
MspI CCGG 3 cut(s) 949, 1212, 1297
MspR9I CCNGG 4 cut(s) 279, 774, 872, 1297
MunI CAATTG 1 cut(s) 972
MvaI CCWGG 3 cut(s) 279, 774, 872
MvnI CGCG 1 cut(s) 619
MwoI GCNNNNNNNGC 9 cut(s) 16, 119, 430, 521, 689, 783, 809, 850, 1041
NaeI GCCGGC 1 cut(s) 950
NciI CCSGG 1 cut(s) 1297
NcoI CCATGG 1 cut(s) 1172
NdeI CATATG 1 cut(s) 323
NdeII GATC 2 cut(s) 97, 819
NgoMIV GCCGGC 1 cut(s) 948
NlaIV GGNNCC 2 cut(s) 32, 99
NmeAIII GCCGAG 1 cut(s) 659
NmuCI GTSAC 1 cut(s) 394
NsiI ATGCAT 1 cut(s) 323
NspI RCATGY 2 cut(s) 221, 812
NspV TTCGAA 1 cut(s) 50
OliI CACNNNNGTG 1 cut(s) 633
PagI TCATGA 1 cut(s) 1315
PauI GCGCGC 1 cut(s) 617
PciSI GCTCTTC 1 cut(s) 1017
PdiI GCCGGC 1 cut(s) 950
PfeI GAWTC 2 cut(s) 758, 1265
PflMI CCANNNNNTGG 1 cut(s) 44
PleI GAGTC 3 cut(s) 694, 895, 1311
PpsI GAGTC 3 cut(s) 694, 895, 1311
Psp6I CCWGG 3 cut(s) 277, 772, 870
PspGI CCWGG 3 cut(s) 277, 772, 870
PspN4I GGNNCC 2 cut(s) 32, 99
PspPI GGNCC 1 cut(s) 785
PsrI GAACNNNNNNTAC 2 cut(s) 486, 518
PstI CTGCAG 2 cut(s) 67, 817
PstNI CAGNNNCTG 1 cut(s) 1262
PsuI RGATCY 1 cut(s) 97
PteI GCGCGC 1 cut(s) 617
RsaI GTAC 3 cut(s) 375, 766, 1047
RsaNI GTAC 3 cut(s) 374, 765, 1046
RseI CAYNNNNRTG 4 cut(s) 207, 322, 534, 633
SalI GTCGAC 1 cut(s) 1091
SapI GCTCTTC 1 cut(s) 1017
SaqAI TTAA 6 cut(s) 135, 236, 303, 359, 624, 1423
Sau3AI GATC 2 cut(s) 97, 819
Sau96I GGNCC 1 cut(s) 785
SchI GAGTC 3 cut(s) 694, 895, 1312
ScrFI CCNGG 4 cut(s) 279, 774, 872, 1297
SfaNI GCATC 2 cut(s) 374, 1309
SfcI CTRYAG 2 cut(s) 63, 813
SfuI TTCGAA 1 cut(s) 50
SmiMI CAYNNNNRTG 4 cut(s) 207, 322, 534, 633
SpeI ACTAGT 1 cut(s) 1234
Sse9I AATT 6 cut(s) 69, 647, 705, 848, 972, 1139
SsiI CCGC 2 cut(s) 470, 1105
SspI AATATT 1 cut(s) 133
SspMI CTAG 6 cut(s) 693, 789, 854, 956, 1235, 1323
StyD4I CCNGG 4 cut(s) 277, 772, 870, 1295
StyI CCWWGG 3 cut(s) 26, 781, 1172
TaaI ACNGT 2 cut(s) 196, 343
TaqI TCGA 5 cut(s) 50, 246, 733, 1092, 1231
TaqII GACCGA 1 cut(s) 1110
TasI AATT 6 cut(s) 69, 647, 705, 848, 972, 1139
TatI WGTACW 2 cut(s) 373, 764
TauI GCSGC 1 cut(s) 473
TfiI GAWTC 2 cut(s) 758, 1265
Tru1I TTAA 6 cut(s) 135, 236, 303, 359, 624, 1423
Tru9I TTAA 6 cut(s) 135, 236, 303, 359, 624, 1423
TscAI CASTG 3 cut(s) 199, 822, 832
TseFI GTSAC 1 cut(s) 394
Tsp45I GTSAC 1 cut(s) 394
TspDTI ATGAA 7 cut(s) 72, 183, 903, 1053, 1106, 1300, 1304
TspRI CASTG 3 cut(s) 199, 822, 832
Van91I CCANNNNNTGG 1 cut(s) 44
XagI CCTNNNNNAGG 1 cut(s) 608
XapI RAATTY 1 cut(s) 1139
XceI RCATGY 2 cut(s) 221, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 788
XmiI GTMKAC 2 cut(s) 283, 1092
XspI CTAG 6 cut(s) 693, 789, 854, 956, 1235, 1323
Zsp2I ATGCAT 1 cut(s) 323
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.