MD14G1013500.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0005474
Physical Location & Seq
Forward (+)
1197295 .. 1199380
2086 bp
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UTR
Exon/CDS
Intron
MD14G1013500.v1.1.491

Sequence Viewer

Length: 405 bp
ATGAAAGGCCAAAGCAGTTACGCTCCACCTGCTTACATTCCGTTGGGAATGTCGGATTCTGAATCAGCGATTGTTTTGCGAAATGAAGACCCGCCTAATCAGCAGAACAGCGTTGACGGGCCCAAACAATGGTCTTCTGGAATCTGCGCTTGCTGTGACGATATGCAGAGCTGTTGTGTAGGTCTATTTTGTCCTTGCTTTCTGTTTGGAAAGAATGCAGAATTTCTGGGTTCTGGGACATTGTTAGGATCATGCATGACTCATTTTATTTTGTGGGCACTTGTTAATACCGCCTGCTGCTTATTGACTGAGGGCCTGTTTTTGGGCATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGTGCCCTAAGAACAAAGTATAATCTTCAGGTATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

135

Amino Acids

14.4

Weight (kDa)

5.11

Isoelectric Point (pI)

66.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 44 - 133 4.6e-17 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 37
Acc36I ACCTGC 1 cut(s) 37
AccB7I CCANNNNNTGG 1 cut(s) 129
AciI CCGC 3 cut(s) 92, 291, 367
AclWI GGATC 1 cut(s) 256
AcsI RAATTY 1 cut(s) 221
AcuI CTGAAG 1 cut(s) 380
AfiI CCNNNNNNNGG 2 cut(s) 129, 322
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
AlwI GGATC 1 cut(s) 256
AoxI GGCC 3 cut(s) 7, 119, 313
ApaI GGGCCC 1 cut(s) 123
ApeKI GCWGC 1 cut(s) 297
ApoI RAATTY 1 cut(s) 221
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 3 cut(s) 119, 120, 313
AsuC2I CCSGG 1 cut(s) 333
BaeGI GKGCMC 3 cut(s) 123, 280, 376
BanII GRGCYC 1 cut(s) 123
BbsI GAAGAC 2 cut(s) 93, 126
BbvI GCAGC 1 cut(s) 284
BcgI CGANNNNNNTGC 2 cut(s) 58, 92
BcnI CCSGG 1 cut(s) 333
BfuAI ACCTGC 1 cut(s) 37
BisI GCNGC 1 cut(s) 298
BlsI GCNGC 1 cut(s) 299
Bme1390I CCNGG 1 cut(s) 333
BmgT120I GGNCC 3 cut(s) 119, 120, 313
BmiI GGNNCC 1 cut(s) 121
BmrFI CCNGG 1 cut(s) 333
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 2 cut(s) 93, 126
BpuMI CCSGG 1 cut(s) 333
Bsc4I CCNNNNNNNGG 2 cut(s) 129, 322
BseGI GGATG 1 cut(s) 341
BseLI CCNNNNNNNGG 2 cut(s) 129, 322
BseMII CTCAG 1 cut(s) 300
BseSI GKGCMC 3 cut(s) 123, 280, 376
BseXI GCAGC 1 cut(s) 284
BshFI GGCC 3 cut(s) 9, 121, 315
BsiSI CCGG 1 cut(s) 332
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 129, 322
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 3 cut(s) 9, 121, 315
Bsp120I GGGCCC 1 cut(s) 119
Bsp1286I GDGCHC 3 cut(s) 123, 280, 376
Bsp143I GATC 1 cut(s) 248
BspACI CCGC 3 cut(s) 92, 291, 367
BspANI GGCC 3 cut(s) 9, 121, 315
BspCNI CTCAG 1 cut(s) 301
BspLI GGNNCC 1 cut(s) 121
BspMI ACCTGC 1 cut(s) 37
BspPI GGATC 1 cut(s) 256
BssMI GATC 1 cut(s) 248
BstAPI GCANNNNNTGC 1 cut(s) 353
BstC8I GCNNGC 2 cut(s) 151, 295
BstDEI CTNAG 2 cut(s) 309, 377
BstF5I GGATG 1 cut(s) 341
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 1 cut(s) 251
BstMBI GATC 1 cut(s) 248
BstMWI GCNNNNNNNGC 3 cut(s) 29, 100, 353
BstNSI RCATGY 1 cut(s) 351
BstSCI CCNGG 1 cut(s) 331
BstSLI GKGCMC 3 cut(s) 123, 280, 376
BstV1I GCAGC 1 cut(s) 284
BstV2I GAAGAC 2 cut(s) 93, 126
BsuRI GGCC 3 cut(s) 9, 121, 315
BtsCI GGATG 1 cut(s) 341
BtsI GCAGTG 1 cut(s) 376
BtsIMutI CAGTG 1 cut(s) 376
BveI ACCTGC 1 cut(s) 37
Cac8I GCNNGC 2 cut(s) 151, 295
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 3 cut(s) 119, 120, 313
CviAII CATG 3 cut(s) 252, 256, 348
CviJI RGCY 5 cut(s) 9, 121, 171, 315, 363
CviKI_1 RGCY 5 cut(s) 9, 121, 171, 315, 363
DdeI CTNAG 2 cut(s) 309, 377
DpnI GATC 1 cut(s) 250
DpnII GATC 1 cut(s) 248
Eco24I GRGCYC 1 cut(s) 123
Eco57I CTGAAG 1 cut(s) 380
EcoO109I RGGNCCY 1 cut(s) 313
EcoT22I ATGCAT 1 cut(s) 257
EcoT38I GRGCYC 1 cut(s) 123
FaeI CATG 3 cut(s) 255, 259, 351
FaiI YATR 8 cut(s) 164, 253, 257, 329, 349, 354, 390, 403
FaqI GGGAC 1 cut(s) 250
FatI CATG 3 cut(s) 251, 255, 347
FauI CCCGC 1 cut(s) 99
Fnu4HI GCNGC 1 cut(s) 298
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 1 cut(s) 123
Fsp4HI GCNGC 1 cut(s) 298
GlaI GCGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 298
HaeIII GGCC 3 cut(s) 9, 121, 315
HapII CCGG 1 cut(s) 332
HhaI GCGC 1 cut(s) 149
Hin1II CATG 3 cut(s) 255, 259, 351
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HincII GTYRAC 1 cut(s) 115
HindII GTYRAC 1 cut(s) 115
HinfI GANTC 4 cut(s) 56, 62, 141, 259
HpaII CCGG 1 cut(s) 332
Hpy166II GTNNAC 1 cut(s) 115
Hpy188I TCNGA 2 cut(s) 55, 61
Hpy188III TCNNGA 1 cut(s) 138
Hpy8I GTNNAC 1 cut(s) 115
HpyCH4V TGCA 4 cut(s) 166, 218, 255, 347
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 100, 353
HpyF3I CTNAG 2 cut(s) 309, 377
Hsp92II CATG 3 cut(s) 255, 259, 351
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 1 cut(s) 248
LmnI GCTCC 1 cut(s) 28
LpnPI CCDG 8 cut(s) 42, 123, 212, 219, 307, 329, 345, 383
Lsp1109I GCAGC 1 cut(s) 284
LweI GCATC 1 cut(s) 326
MaeIII GTNAC 2 cut(s) 17, 155
MalI GATC 1 cut(s) 250
MboI GATC 1 cut(s) 248
MboII GAAGA 3 cut(s) 98, 126, 386
MhlI GDGCHC 3 cut(s) 123, 280, 376
MluCI AATT 1 cut(s) 221
MlyI GAGTC 1 cut(s) 253
MmeI TCCRAC 1 cut(s) 33
MnlI CCTC 1 cut(s) 304
Mph1103I ATGCAT 1 cut(s) 257
MseI TTAA 1 cut(s) 285
MspI CCGG 1 cut(s) 332
MspR9I CCNGG 1 cut(s) 333
Mva1269I GAATGC 1 cut(s) 220
MwoI GCNNNNNNNGC 3 cut(s) 29, 100, 353
NciI CCSGG 1 cut(s) 333
NdeII GATC 1 cut(s) 248
NlaIII CATG 3 cut(s) 255, 259, 351
NlaIV GGNNCC 1 cut(s) 121
NmuCI GTSAC 1 cut(s) 155
NsiI ATGCAT 1 cut(s) 257
NspI RCATGY 1 cut(s) 351
PaqCI CACCTGC 1 cut(s) 37
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 56, 62, 141
PflMI CCANNNNNTGG 1 cut(s) 129
PkrI GCNGC 1 cut(s) 299
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PspN4I GGNNCC 1 cut(s) 121
PspOMI GGGCCC 1 cut(s) 119
PspPI GGNCC 3 cut(s) 119, 120, 313
SaqAI TTAA 1 cut(s) 285
SatI GCNGC 1 cut(s) 298
Sau3AI GATC 1 cut(s) 248
Sau96I GGNCC 3 cut(s) 119, 120, 313
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 1 cut(s) 333
SduI GDGCHC 3 cut(s) 123, 280, 376
SetI ASST 4 cut(s) 31, 173, 184, 402
SfaNI GCATC 1 cut(s) 326
Sse9I AATT 1 cut(s) 221
SsiI CCGC 3 cut(s) 92, 291, 367
StyD4I CCNGG 1 cut(s) 331
TasI AATT 1 cut(s) 221
TfiI GAWTC 3 cut(s) 56, 62, 141
Tru1I TTAA 1 cut(s) 285
Tru9I TTAA 1 cut(s) 285
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 1 cut(s) 155
TseI GCWGC 1 cut(s) 297
Tsp45I GTSAC 1 cut(s) 155
TspDTI ATGAA 2 cut(s) 17, 99
TspGWI ACGGA 1 cut(s) 30
TspRI CASTG 1 cut(s) 376
Van91I CCANNNNNTGG 1 cut(s) 129
XapI RAATTY 1 cut(s) 221
XceI RCATGY 1 cut(s) 351
Zsp2I ATGCAT 1 cut(s) 257
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.