Rh3BG355100

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3B
OG0005474
Physical Location & Seq
Reverse (-)
42125199 .. 42132321
7123 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3BG355100.1

Sequence Viewer

Length: 747 bp
ATGCTGGAAAGCGAAAGAGGAGGAGAAGGAGACGGCGCTGGTGCAGCTAGCTGCAACGGCGGGACGACTGCGAAATCGTGGGGTCAAGCTTCCAGACAAAAACGGAGAGGAAATTGTAGGTTTCTCCACCGACACAGACGGGGTACTGCGTACAGATCATTTGTTCGTCGCAGAAAGATGAAGAGCCAAAGTAGTTATGCACCTCCGTCTTATATTCCCTTGGGCCAATCAGATTCGGGATCACCTGTTGTTTTACGCAATGAAGACCCTCCCAAGCAGCAAAATAATGTCGATGGGCCGATACAATGGTCTTCTGGAATTTGCGCATGTTGTGATGATACGCAGAGCTGTTGTATAGGTTTATTTTGTCCTTGCTATCTGTTCGGAAAGAATGCAGAGTTTCTGGGCTCTGGGACATTAATGGGCTCCTGCACGACTCATTTTATTCTGTGGGTACTTGTTAATACCGTCTGCTGCTTATTAACTGATGGCCTTATTTTGGGTATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGGGCACTAAGATCAAAATATAATCTCCAGGAAGCACCGTGTGGAGACTTTATTACTCATTTTTTCTGCCATCTGTGCGCAATTTGTCAAGAGTACAGGGAGATCCGCGAAAAGTCTTCTGAATCTGGCCCTGGTGATCTGAATCTAGCCATAGTTACAGCCCCAGCAGTCCAGACAATGGAACCAGGGCTTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

248

Amino Acids

27.05

Weight (kDa)

8.5

Isoelectric Point (pI)

65.46

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 103 - 216 2e-28 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 2 cut(s) 325, 625
AccB7I CCANNNNNTGG 1 cut(s) 724
AccI GTMKAC 1 cut(s) 505
AccII CGCG 1 cut(s) 654
AciI CCGC 3 cut(s) 60, 544, 652
AclWI GGATC 2 cut(s) 247, 643
AcsI RAATTY 1 cut(s) 318
AdeI CACNNNGTG 1 cut(s) 587
AfaI GTAC 4 cut(s) 145, 152, 456, 641
AfiI CCNNNNNNNGG 2 cut(s) 499, 724
AjnI CCWGG 3 cut(s) 573, 676, 730
AluBI AGCT 4 cut(s) 47, 51, 89, 348
AluI AGCT 4 cut(s) 47, 51, 89, 348
Alw26I GTCTC 2 cut(s) 24, 585
AlwI GGATC 2 cut(s) 247, 643
AoxI GGCC 4 cut(s) 223, 296, 490, 673
ApeKI GCWGC 4 cut(s) 44, 51, 277, 474
ApoI RAATTY 1 cut(s) 318
AseI ATTAAT 1 cut(s) 419
AspLEI GCGC 3 cut(s) 38, 326, 626
AspS9I GGNCC 3 cut(s) 223, 296, 674
AsuC2I CCSGG 1 cut(s) 510
AsuHPI GGTGA 2 cut(s) 234, 692
AsuNHI GCTAGC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 553
BaeI ACNNNNGTAYC 2 cut(s) 293, 326
BanII GRGCYC 2 cut(s) 410, 428
BbsI GAAGAC 3 cut(s) 270, 303, 654
BbvI GCAGC 4 cut(s) 38, 56, 289, 461
BccI CCATC 3 cut(s) 287, 482, 624
BceAI ACGGC 2 cut(s) 49, 73
BciT130I CCWGG 3 cut(s) 575, 678, 732
BcnI CCSGG 1 cut(s) 510
BcoDI GTCTC 2 cut(s) 24, 585
BfaI CTAG 2 cut(s) 48, 692
BfoI RGCGCY 1 cut(s) 39
BisI GCNGC 4 cut(s) 45, 52, 278, 475
BlsI GCNGC 4 cut(s) 46, 53, 279, 476
Bme1390I CCNGG 4 cut(s) 510, 575, 678, 732
BmgT120I GGNCC 3 cut(s) 223, 296, 674
BmiI GGNNCC 2 cut(s) 427, 729
BmrFI CCNGG 4 cut(s) 510, 575, 678, 732
BmsI GCATC 1 cut(s) 503
BmtI GCTAGC 1 cut(s) 51
BpiI GAAGAC 3 cut(s) 270, 303, 654
BpmI CTGGAG 1 cut(s) 557
BpuMI CCSGG 1 cut(s) 510
BsaBI GATNNNNATC 1 cut(s) 687
BsaJI CCNNGG 3 cut(s) 219, 676, 731
Bsc4I CCNNNNNNNGG 2 cut(s) 499, 724
Bse3DI GCAATG 1 cut(s) 265
Bse8I GATNNNNATC 1 cut(s) 687
BseBI CCWGG 3 cut(s) 575, 678, 732
BseDI CCNNGG 3 cut(s) 219, 676, 731
BseGI GGATG 1 cut(s) 518
BseJI GATNNNNATC 1 cut(s) 687
BseLI CCNNNNNNNGG 2 cut(s) 499, 724
BseMI GCAATG 1 cut(s) 265
BseRI GAGGAG 2 cut(s) 33, 36
BseSI GKGCMC 1 cut(s) 553
BseXI GCAGC 4 cut(s) 38, 56, 289, 461
BseYI CCCAGC 1 cut(s) 709
BsgI GTGCAG 2 cut(s) 63, 415
Bsh1236I CGCG 1 cut(s) 654
BshFI GGCC 4 cut(s) 225, 298, 492, 675
BsiSI CCGG 1 cut(s) 509
BslFI GGGAC 2 cut(s) 76, 427
BslI CCNNNNNNNGG 2 cut(s) 499, 724
BsmAI GTCTC 2 cut(s) 24, 585
BsmBI CGTCTC 1 cut(s) 24
BsmFI GGGAC 2 cut(s) 76, 427
BsmI GAATGC 1 cut(s) 397
BsnI GGCC 4 cut(s) 225, 298, 492, 675
Bsp1286I GDGCHC 3 cut(s) 410, 428, 553
Bsp143I GATC 5 cut(s) 155, 239, 557, 648, 682
BspACI CCGC 3 cut(s) 60, 544, 652
BspANI GGCC 4 cut(s) 225, 298, 492, 675
BspFNI CGCG 1 cut(s) 654
BspLI GGNNCC 2 cut(s) 427, 729
BspOI GCTAGC 1 cut(s) 51
BspPI GGATC 2 cut(s) 247, 643
BspQI GCTCTTC 1 cut(s) 176
BsrDI GCAATG 1 cut(s) 265
BssECI CCNNGG 3 cut(s) 219, 676, 731
BssMI GATC 5 cut(s) 155, 239, 557, 648, 682
BssNAI GTATAC 1 cut(s) 506
BssT1I CCWWGG 1 cut(s) 219
Bst1107I GTATAC 1 cut(s) 506
Bst2UI CCWGG 3 cut(s) 575, 678, 732
Bst4CI ACNGT 2 cut(s) 469, 585
Bst6I CTCTTC 1 cut(s) 176
BstAPI GCANNNNNTGC 1 cut(s) 530
BstC8I GCNNGC 1 cut(s) 49
BstDEI CTNAG 1 cut(s) 554
BstF5I GGATG 1 cut(s) 518
BstFNI CGCG 1 cut(s) 654
BstH2I RGCGCY 1 cut(s) 39
BstHHI GCGC 3 cut(s) 38, 326, 626
BstKTI GATC 5 cut(s) 158, 242, 560, 651, 685
BstMAI GTCTC 2 cut(s) 24, 585
BstMBI GATC 5 cut(s) 155, 239, 557, 648, 682
BstMWI GCNNNNNNNGC 4 cut(s) 44, 57, 530, 621
BstNI CCWGG 3 cut(s) 575, 678, 732
BstNSI RCATGY 2 cut(s) 330, 528
BstSCI CCNGG 4 cut(s) 508, 573, 676, 730
BstSLI GKGCMC 1 cut(s) 553
BstUI CGCG 1 cut(s) 654
BstV1I GCAGC 4 cut(s) 38, 56, 289, 461
BstV2I GAAGAC 3 cut(s) 270, 303, 654
BstX2I RGATCY 1 cut(s) 648
BstYI RGATCY 1 cut(s) 648
BstZ17I GTATAC 1 cut(s) 506
BsuRI GGCC 4 cut(s) 225, 298, 492, 675
BtsCI GGATG 1 cut(s) 518
Cac8I GCNNGC 1 cut(s) 49
CfoI GCGC 3 cut(s) 38, 326, 626
Cfr13I GGNCC 3 cut(s) 223, 296, 674
Csp6I GTAC 4 cut(s) 144, 151, 455, 640
CviAII CATG 2 cut(s) 327, 525
CviQI GTAC 4 cut(s) 144, 151, 455, 640
DdeI CTNAG 1 cut(s) 554
DpnI GATC 5 cut(s) 157, 241, 559, 650, 684
DpnII GATC 5 cut(s) 155, 239, 557, 648, 682
DraIII CACNNNGTG 1 cut(s) 587
Eam1104I CTCTTC 1 cut(s) 176
EarI CTCTTC 1 cut(s) 176
Eco130I CCWWGG 1 cut(s) 219
Eco24I GRGCYC 2 cut(s) 410, 428
EcoRII CCWGG 3 cut(s) 573, 676, 730
EcoT14I CCWWGG 1 cut(s) 219
EcoT38I GRGCYC 2 cut(s) 410, 428
ErhI CCWWGG 1 cut(s) 219
Esp3I CGTCTC 1 cut(s) 24
FaeI CATG 2 cut(s) 330, 528
FaiI YATR 9 cut(s) 198, 213, 328, 356, 506, 526, 531, 567, 698
FaqI GGGAC 2 cut(s) 76, 427
FatI CATG 2 cut(s) 326, 524
FauI CCCGC 1 cut(s) 53
FblI GTMKAC 1 cut(s) 505
Fnu4HI GCNGC 4 cut(s) 45, 52, 278, 475
FokI GGATG 1 cut(s) 525
FriOI GRGCYC 2 cut(s) 410, 428
Fsp4HI GCNGC 4 cut(s) 45, 52, 278, 475
FspBI CTAG 2 cut(s) 48, 692
FspI TGCGCA 2 cut(s) 325, 625
GlaI GCGC 3 cut(s) 37, 325, 625
GluI GCNGC 4 cut(s) 45, 52, 278, 475
GsaI CCCAGC 1 cut(s) 713
GsuI CTGGAG 1 cut(s) 557
HaeII RGCGCY 1 cut(s) 39
HaeIII GGCC 4 cut(s) 225, 298, 492, 675
HapII CCGG 1 cut(s) 509
HhaI GCGC 3 cut(s) 38, 326, 626
Hin1II CATG 2 cut(s) 330, 528
Hin6I GCGC 3 cut(s) 36, 324, 624
HinP1I GCGC 3 cut(s) 36, 324, 624
HindIII AAGCTT 1 cut(s) 87
HinfI GANTC 4 cut(s) 233, 436, 668, 688
HpaII CCGG 1 cut(s) 509
HphI GGTGA 2 cut(s) 234, 692
Hpy166II GTNNAC 1 cut(s) 506
Hpy188I TCNGA 4 cut(s) 232, 386, 667, 687
Hpy188III TCNNGA 5 cut(s) 93, 237, 315, 635, 718
Hpy8I GTNNAC 1 cut(s) 506
Hpy99I CGWCG 1 cut(s) 171
HpyAV CCTTC 1 cut(s) 20
HpyCH4III ACNGT 2 cut(s) 469, 585
HpyCH4V TGCA 6 cut(s) 44, 54, 200, 395, 432, 524
HpyF10VI GCNNNNNNNGC 4 cut(s) 44, 57, 530, 621
HpyF3I CTNAG 1 cut(s) 554
Hsp92II CATG 2 cut(s) 330, 528
HspAI GCGC 3 cut(s) 36, 324, 624
Kzo9I GATC 5 cut(s) 155, 239, 557, 648, 682
LguI GCTCTTC 1 cut(s) 176
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 4 cut(s) 38, 56, 289, 461
LweI GCATC 1 cut(s) 503
MaeI CTAG 2 cut(s) 48, 692
MaeIII GTNAC 1 cut(s) 700
MalI GATC 5 cut(s) 157, 241, 559, 650, 684
MboI GATC 5 cut(s) 155, 239, 557, 648, 682
MboII GAAGA 4 cut(s) 193, 275, 303, 654
MflI RGATCY 1 cut(s) 648
MhlI GDGCHC 3 cut(s) 410, 428, 553
MluCI AATT 3 cut(s) 112, 318, 627
MlyI GAGTC 1 cut(s) 430
MnlI CCTC 5 cut(s) 11, 14, 101, 213, 279
MseI TTAA 3 cut(s) 419, 462, 482
MspI CCGG 1 cut(s) 509
MspR9I CCNGG 4 cut(s) 510, 575, 678, 732
Mva1269I GAATGC 1 cut(s) 397
MvaI CCWGG 3 cut(s) 575, 678, 732
MvnI CGCG 1 cut(s) 654
MwoI GCNNNNNNNGC 4 cut(s) 44, 57, 530, 621
NciI CCSGG 1 cut(s) 510
NdeII GATC 5 cut(s) 155, 239, 557, 648, 682
NheI GCTAGC 1 cut(s) 47
NlaIII CATG 2 cut(s) 330, 528
NlaIV GGNNCC 2 cut(s) 427, 729
NsbI TGCGCA 2 cut(s) 325, 625
NspI RCATGY 2 cut(s) 330, 528
PciSI GCTCTTC 1 cut(s) 176
PctI GAATGC 1 cut(s) 397
PfeI GAWTC 3 cut(s) 233, 668, 688
PflMI CCANNNNNTGG 1 cut(s) 724
PfoI TCCNGGA 1 cut(s) 573
PkrI GCNGC 4 cut(s) 46, 53, 279, 476
PleI GAGTC 1 cut(s) 430
PpsI GAGTC 1 cut(s) 430
PshBI ATTAAT 1 cut(s) 419
Psp6I CCWGG 3 cut(s) 573, 676, 730
PspFI CCCAGC 1 cut(s) 709
PspGI CCWGG 3 cut(s) 573, 676, 730
PspN4I GGNNCC 2 cut(s) 427, 729
PspPI GGNCC 3 cut(s) 223, 296, 674
PsuI RGATCY 1 cut(s) 648
RsaI GTAC 4 cut(s) 145, 152, 456, 641
RsaNI GTAC 4 cut(s) 144, 151, 455, 640
SapI GCTCTTC 1 cut(s) 176
SaqAI TTAA 3 cut(s) 419, 462, 482
SatI GCNGC 4 cut(s) 45, 52, 278, 475
Sau3AI GATC 5 cut(s) 155, 239, 557, 648, 682
Sau96I GGNCC 3 cut(s) 223, 296, 674
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 4 cut(s) 510, 575, 678, 732
SduI GDGCHC 3 cut(s) 410, 428, 553
SetI ASST 8 cut(s) 49, 53, 91, 122, 205, 247, 350, 361
SfaNI GCATC 1 cut(s) 503
Sse9I AATT 3 cut(s) 112, 318, 627
SsiI CCGC 3 cut(s) 60, 544, 652
SspMI CTAG 2 cut(s) 48, 692
StyD4I CCNGG 4 cut(s) 508, 573, 676, 730
StyI CCWWGG 1 cut(s) 219
TaaI ACNGT 2 cut(s) 469, 585
TaqI TCGA 1 cut(s) 291
TasI AATT 3 cut(s) 112, 318, 627
TatI WGTACW 1 cut(s) 639
TfiI GAWTC 3 cut(s) 233, 668, 688
Tru1I TTAA 3 cut(s) 419, 462, 482
Tru9I TTAA 3 cut(s) 419, 462, 482
TseI GCWGC 4 cut(s) 44, 51, 277, 474
TspDTI ATGAA 2 cut(s) 194, 276
TspGWI ACGGA 2 cut(s) 118, 195
Van91I CCANNNNNTGG 1 cut(s) 724
VspI ATTAAT 1 cut(s) 419
XapI RAATTY 1 cut(s) 318
XceI RCATGY 2 cut(s) 330, 528
XmiI GTMKAC 1 cut(s) 505
XspI CTAG 2 cut(s) 48, 692
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.