Rh3DG354100

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005474
Physical Location & Seq
Reverse (-)
42834596 .. 42837015
2420 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG354100.1

Sequence Viewer

Length: 570 bp
ATGAAGAGCCAAAGCAGTTATGCACCTCCGTCTTATATTCCCTTGGGCCAATCAGATTCGGGATCACCTGTTGTTTTACGCAATGAAGACCCTCCCAAGCAGCAAAATAATGTCGATCGGCCGATACAATGGTCTTCTGGAATTTGCGCATGTTGTGATGATACGCAGAGCTGTTGTATAGGTTTATTTTGTCCTTGCTATCTGTTCGGAAAGAATGCAGAGTTTCTGGGCTCTGGGACATTAATGGGCTCCTGCACGACTCATTTTATTCTGTGGGTACTTGTTAATACCGTCTGTTGCTTATTAACTGATGGCCTTATTTTGGGTATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGGGCACTAAGATCAAAATATAATCTCCAGGAAGCACCGTGTGGAGACTTTATTACTCATTTTTTCTGCCATCTGTGTGCAATTTGTCAAGAGTACCGGGAGATCCGTGAAAAGTCTGCTGAATCTGGCCCTGGTGATCTGAATCTAGCCATAGTTACAGCCCCAGCAGTCCAGACAATGGAACCAGGGCTTGACAAGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

189

Amino Acids

20.57

Weight (kDa)

5.29

Isoelectric Point (pI)

61.71

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 44 - 157 9.7e-29 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc16I TGCGCA 1 cut(s) 148
AccB7I CCANNNNNTGG 1 cut(s) 547
AccI GTMKAC 1 cut(s) 328
AciI CCGC 1 cut(s) 367
AclWI GGATC 2 cut(s) 70, 466
AcoI YGGCCR 1 cut(s) 119
AcsI RAATTY 1 cut(s) 141
AdeI CACNNNGTG 1 cut(s) 410
AfaI GTAC 2 cut(s) 279, 464
AfiI CCNNNNNNNGG 2 cut(s) 322, 547
AjnI CCWGG 3 cut(s) 396, 499, 553
AluBI AGCT 1 cut(s) 171
AluI AGCT 1 cut(s) 171
Alw26I GTCTC 1 cut(s) 408
AlwI GGATC 2 cut(s) 70, 466
AoxI GGCC 4 cut(s) 46, 119, 313, 496
ApeKI GCWGC 1 cut(s) 100
ApoI RAATTY 1 cut(s) 141
AseI ATTAAT 1 cut(s) 242
AspLEI GCGC 1 cut(s) 149
AspS9I GGNCC 2 cut(s) 46, 497
AsuC2I CCSGG 2 cut(s) 333, 467
AsuHPI GGTGA 2 cut(s) 57, 515
BaeGI GKGCMC 1 cut(s) 376
BaeI ACNNNNGTAYC 2 cut(s) 116, 149
BanII GRGCYC 2 cut(s) 233, 251
BbsI GAAGAC 2 cut(s) 93, 126
BbvI GCAGC 1 cut(s) 112
BccI CCATC 2 cut(s) 305, 447
BciT130I CCWGG 3 cut(s) 398, 501, 555
BcnI CCSGG 2 cut(s) 333, 467
BcoDI GTCTC 1 cut(s) 408
BfaI CTAG 1 cut(s) 515
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 5 cut(s) 333, 398, 467, 501, 555
BmgT120I GGNCC 2 cut(s) 46, 497
BmiI GGNNCC 2 cut(s) 250, 552
BmrFI CCNGG 5 cut(s) 333, 398, 467, 501, 555
BmsI GCATC 1 cut(s) 326
BpiI GAAGAC 2 cut(s) 93, 126
BpmI CTGGAG 1 cut(s) 380
BpuMI CCSGG 2 cut(s) 333, 467
BsaBI GATNNNNATC 1 cut(s) 510
BsaJI CCNNGG 3 cut(s) 42, 499, 554
Bsc4I CCNNNNNNNGG 2 cut(s) 322, 547
Bse3DI GCAATG 1 cut(s) 88
Bse8I GATNNNNATC 1 cut(s) 510
BseBI CCWGG 3 cut(s) 398, 501, 555
BseDI CCNNGG 3 cut(s) 42, 499, 554
BseGI GGATG 1 cut(s) 341
BseJI GATNNNNATC 1 cut(s) 510
BseLI CCNNNNNNNGG 2 cut(s) 322, 547
BseMI GCAATG 1 cut(s) 88
BseSI GKGCMC 1 cut(s) 376
BseX3I CGGCCG 1 cut(s) 119
BseXI GCAGC 1 cut(s) 112
BseYI CCCAGC 1 cut(s) 532
BsgI GTGCAG 1 cut(s) 238
Bsh1285I CGRYCG 2 cut(s) 118, 122
BshFI GGCC 4 cut(s) 48, 121, 315, 498
BsiEI CGRYCG 2 cut(s) 118, 122
BsiSI CCGG 2 cut(s) 332, 466
BslFI GGGAC 1 cut(s) 250
BslI CCNNNNNNNGG 2 cut(s) 322, 547
BsmAI GTCTC 1 cut(s) 408
BsmFI GGGAC 1 cut(s) 250
BsmI GAATGC 1 cut(s) 220
BsnI GGCC 4 cut(s) 48, 121, 315, 498
Bsp1286I GDGCHC 3 cut(s) 233, 251, 376
Bsp143I GATC 5 cut(s) 62, 115, 380, 471, 505
BspACI CCGC 1 cut(s) 367
BspANI GGCC 4 cut(s) 48, 121, 315, 498
BspLI GGNNCC 2 cut(s) 250, 552
BspPI GGATC 2 cut(s) 70, 466
BsrDI GCAATG 1 cut(s) 88
BssECI CCNNGG 3 cut(s) 42, 499, 554
BssMI GATC 5 cut(s) 62, 115, 380, 471, 505
BssNAI GTATAC 1 cut(s) 329
BssT1I CCWWGG 1 cut(s) 42
Bst1107I GTATAC 1 cut(s) 329
Bst2UI CCWGG 3 cut(s) 398, 501, 555
Bst4CI ACNGT 2 cut(s) 292, 408
BstAPI GCANNNNNTGC 1 cut(s) 353
BstDEI CTNAG 1 cut(s) 377
BstF5I GGATG 1 cut(s) 341
BstHHI GCGC 1 cut(s) 149
BstKTI GATC 5 cut(s) 65, 118, 383, 474, 508
BstMAI GTCTC 1 cut(s) 408
BstMBI GATC 5 cut(s) 62, 115, 380, 471, 505
BstMCI CGRYCG 2 cut(s) 118, 122
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNI CCWGG 3 cut(s) 398, 501, 555
BstNSI RCATGY 2 cut(s) 153, 351
BstSCI CCNGG 5 cut(s) 331, 396, 465, 499, 553
BstSLI GKGCMC 1 cut(s) 376
BstV1I GCAGC 1 cut(s) 112
BstV2I GAAGAC 2 cut(s) 93, 126
BstX2I RGATCY 1 cut(s) 471
BstYI RGATCY 1 cut(s) 471
BstZ17I GTATAC 1 cut(s) 329
BstZI CGGCCG 1 cut(s) 119
BsuRI GGCC 4 cut(s) 48, 121, 315, 498
BtsCI GGATG 1 cut(s) 341
CfoI GCGC 1 cut(s) 149
Cfr13I GGNCC 2 cut(s) 46, 497
Csp6I GTAC 2 cut(s) 278, 463
CviAII CATG 2 cut(s) 150, 348
CviQI GTAC 2 cut(s) 278, 463
DdeI CTNAG 1 cut(s) 377
DpnI GATC 5 cut(s) 64, 117, 382, 473, 507
DpnII GATC 5 cut(s) 62, 115, 380, 471, 505
DraIII CACNNNGTG 1 cut(s) 410
EaeI YGGCCR 1 cut(s) 119
EagI CGGCCG 1 cut(s) 119
EclXI CGGCCG 1 cut(s) 119
Eco130I CCWWGG 1 cut(s) 42
Eco24I GRGCYC 2 cut(s) 233, 251
Eco52I CGGCCG 1 cut(s) 119
EcoRII CCWGG 3 cut(s) 396, 499, 553
EcoT14I CCWWGG 1 cut(s) 42
EcoT38I GRGCYC 2 cut(s) 233, 251
ErhI CCWWGG 1 cut(s) 42
FaeI CATG 2 cut(s) 153, 351
FaiI YATR 9 cut(s) 21, 36, 151, 179, 329, 349, 354, 390, 521
FaqI GGGAC 1 cut(s) 250
FatI CATG 2 cut(s) 149, 347
FblI GTMKAC 1 cut(s) 328
Fnu4HI GCNGC 1 cut(s) 101
FokI GGATG 1 cut(s) 348
FriOI GRGCYC 2 cut(s) 233, 251
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 1 cut(s) 515
FspI TGCGCA 1 cut(s) 148
GlaI GCGC 1 cut(s) 148
GluI GCNGC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 536
GsuI CTGGAG 1 cut(s) 380
HaeIII GGCC 4 cut(s) 48, 121, 315, 498
HapII CCGG 2 cut(s) 332, 466
HhaI GCGC 1 cut(s) 149
Hin1II CATG 2 cut(s) 153, 351
Hin6I GCGC 1 cut(s) 147
HinP1I GCGC 1 cut(s) 147
HinfI GANTC 4 cut(s) 56, 259, 491, 511
HpaII CCGG 2 cut(s) 332, 466
HphI GGTGA 2 cut(s) 57, 515
Hpy166II GTNNAC 1 cut(s) 329
Hpy188I TCNGA 3 cut(s) 55, 209, 510
Hpy188III TCNNGA 4 cut(s) 60, 138, 458, 541
Hpy8I GTNNAC 1 cut(s) 329
HpyCH4III ACNGT 2 cut(s) 292, 408
HpyCH4V TGCA 5 cut(s) 23, 218, 255, 347, 449
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 1 cut(s) 377
Hsp92II CATG 2 cut(s) 153, 351
HspAI GCGC 1 cut(s) 147
Kzo9I GATC 5 cut(s) 62, 115, 380, 471, 505
LmnI GCTCC 1 cut(s) 254
Lsp1109I GCAGC 1 cut(s) 112
LweI GCATC 1 cut(s) 326
MaeI CTAG 1 cut(s) 515
MaeIII GTNAC 1 cut(s) 523
MalI GATC 5 cut(s) 64, 117, 382, 473, 507
MboI GATC 5 cut(s) 62, 115, 380, 471, 505
MboII GAAGA 3 cut(s) 16, 98, 126
MflI RGATCY 1 cut(s) 471
MhlI GDGCHC 3 cut(s) 233, 251, 376
MluCI AATT 2 cut(s) 141, 450
MlyI GAGTC 1 cut(s) 253
MnlI CCTC 2 cut(s) 36, 102
MseI TTAA 3 cut(s) 242, 285, 305
MslI CAYNNNNRTG 1 cut(s) 444
MspI CCGG 2 cut(s) 332, 466
MspR9I CCNGG 5 cut(s) 333, 398, 467, 501, 555
Mva1269I GAATGC 1 cut(s) 220
MvaI CCWGG 3 cut(s) 398, 501, 555
MwoI GCNNNNNNNGC 1 cut(s) 353
NciI CCSGG 2 cut(s) 333, 467
NdeII GATC 5 cut(s) 62, 115, 380, 471, 505
NlaIII CATG 2 cut(s) 153, 351
NlaIV GGNNCC 2 cut(s) 250, 552
NsbI TGCGCA 1 cut(s) 148
NspI RCATGY 2 cut(s) 153, 351
PctI GAATGC 1 cut(s) 220
PfeI GAWTC 3 cut(s) 56, 491, 511
PflMI CCANNNNNTGG 1 cut(s) 547
PfoI TCCNGGA 1 cut(s) 396
PkrI GCNGC 1 cut(s) 102
Ple19I CGATCG 1 cut(s) 118
PleI GAGTC 1 cut(s) 253
PpsI GAGTC 1 cut(s) 253
PshBI ATTAAT 1 cut(s) 242
Psp6I CCWGG 3 cut(s) 396, 499, 553
PspFI CCCAGC 1 cut(s) 532
PspGI CCWGG 3 cut(s) 396, 499, 553
PspN4I GGNNCC 2 cut(s) 250, 552
PspPI GGNCC 2 cut(s) 46, 497
PsuI RGATCY 1 cut(s) 471
PvuI CGATCG 1 cut(s) 118
RsaI GTAC 2 cut(s) 279, 464
RsaNI GTAC 2 cut(s) 278, 463
RseI CAYNNNNRTG 1 cut(s) 444
SaqAI TTAA 3 cut(s) 242, 285, 305
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 5 cut(s) 62, 115, 380, 471, 505
Sau96I GGNCC 2 cut(s) 46, 497
SchI GAGTC 1 cut(s) 253
ScrFI CCNGG 5 cut(s) 333, 398, 467, 501, 555
SduI GDGCHC 3 cut(s) 233, 251, 376
SetI ASST 4 cut(s) 28, 70, 173, 184
SfaNI GCATC 1 cut(s) 326
SmiMI CAYNNNNRTG 1 cut(s) 444
Sse9I AATT 2 cut(s) 141, 450
SsiI CCGC 1 cut(s) 367
SspMI CTAG 1 cut(s) 515
StyD4I CCNGG 5 cut(s) 331, 396, 465, 499, 553
StyI CCWWGG 1 cut(s) 42
TaaI ACNGT 2 cut(s) 292, 408
TaqI TCGA 1 cut(s) 114
TasI AATT 2 cut(s) 141, 450
TfiI GAWTC 3 cut(s) 56, 491, 511
Tru1I TTAA 3 cut(s) 242, 285, 305
Tru9I TTAA 3 cut(s) 242, 285, 305
TseI GCWGC 1 cut(s) 100
TspDTI ATGAA 2 cut(s) 17, 99
TspGWI ACGGA 2 cut(s) 18, 464
Van91I CCANNNNNTGG 1 cut(s) 547
VspI ATTAAT 1 cut(s) 242
XapI RAATTY 1 cut(s) 141
XceI RCATGY 2 cut(s) 153, 351
XmiI GTMKAC 1 cut(s) 328
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.