MD12G1015600.v1.1

Protein PLANT CADMIUM RESISTANCE

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0005474
Physical Location & Seq
Forward (+)
1569148 .. 1570396
1249 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1015600.v1.1.491

Sequence Viewer

Length: 312 bp
ATGACTCATTTTATTTTGTGGGCACTGGTTAATACTGTCTGCTGCTTATTGACTGAGGGTCTGTTTTTGGGCATACCGGGATGCTTTGTTGCATGTTATGCTTGTGGCTACCGCAGTGCCCTAAGAACAAAGTATAATCTTCAGGAAGCGCCGTGTGGAGACTTTGTCACTCATTTTTTCTGCCATCTGTGTGCCATTTGTCAAGAGTACCGAGAGATCCGTGAAAAGTCTGATTCCGGCCCTCCTGACCTGACTCTGGCTGTAGTTACAGCCCCGTCAGTCCAGACAATGGAACCAGGTCTCCAAATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
PLAC8

Protein Analysis

104

Amino Acids

11.43

Weight (kDa)

5.43

Isoelectric Point (pI)

46.74

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PLAC8 PF04749 12 - 72 1.2e-15 PLAC8 family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 289
AciI CCGC 1 cut(s) 112
AclWI GGATC 1 cut(s) 211
AcuI CTGAAG 1 cut(s) 125
AfaI GTAC 1 cut(s) 209
AfiI CCNNNNNNNGG 2 cut(s) 256, 289
AhdI GACNNNNNGTC 1 cut(s) 57
AjnI CCWGG 1 cut(s) 295
AloI GAACNNNNNNTCC 1 cut(s) 285
Alw26I GTCTC 2 cut(s) 153, 305
AlwI GGATC 1 cut(s) 211
AoxI GGCC 1 cut(s) 238
ApeKI GCWGC 1 cut(s) 42
AspLEI GCGC 1 cut(s) 151
AspS9I GGNCC 1 cut(s) 239
AsuC2I CCSGG 1 cut(s) 78
BaeGI GKGCMC 2 cut(s) 25, 121
BbvI GCAGC 1 cut(s) 29
BccI CCATC 1 cut(s) 192
BceAI ACGGC 1 cut(s) 136
BciT130I CCWGG 1 cut(s) 297
BcnI CCSGG 1 cut(s) 78
BcoDI GTCTC 2 cut(s) 153, 305
BfmI CTRYAG 1 cut(s) 261
BfoI RGCGCY 1 cut(s) 152
BisI GCNGC 1 cut(s) 43
BlsI GCNGC 1 cut(s) 44
Bme1390I CCNGG 2 cut(s) 78, 297
BmeRI GACNNNNNGTC 1 cut(s) 57
BmgT120I GGNCC 1 cut(s) 239
BmiI GGNNCC 1 cut(s) 294
BmrFI CCNGG 2 cut(s) 78, 297
BmsI GCATC 1 cut(s) 71
BpuMI CCSGG 1 cut(s) 78
BsaI GGTCTC 1 cut(s) 305
BsaXI ACNNNNNCTCC 3 cut(s) 150, 180, 285
Bsc4I CCNNNNNNNGG 2 cut(s) 256, 289
Bse1I ACTGG 1 cut(s) 30
BseBI CCWGG 1 cut(s) 297
BseGI GGATG 1 cut(s) 86
BseLI CCNNNNNNNGG 2 cut(s) 256, 289
BseMII CTCAG 1 cut(s) 45
BseNI ACTGG 1 cut(s) 30
BseSI GKGCMC 2 cut(s) 25, 121
BseXI GCAGC 1 cut(s) 29
BshFI GGCC 1 cut(s) 240
BsiSI CCGG 2 cut(s) 77, 237
BslI CCNNNNNNNGG 2 cut(s) 256, 289
BsmAI GTCTC 2 cut(s) 153, 305
BsnI GGCC 1 cut(s) 240
Bso31I GGTCTC 1 cut(s) 305
Bsp1286I GDGCHC 2 cut(s) 25, 121
Bsp143I GATC 1 cut(s) 216
BspACI CCGC 1 cut(s) 112
BspANI GGCC 1 cut(s) 240
BspCNI CTCAG 1 cut(s) 46
BspLI GGNNCC 1 cut(s) 294
BspPI GGATC 1 cut(s) 211
BspTNI GGTCTC 1 cut(s) 305
BsrI ACTGG 1 cut(s) 30
BssMI GATC 1 cut(s) 216
Bst2UI CCWGG 1 cut(s) 297
Bst4CI ACNGT 1 cut(s) 37
BstAPI GCANNNNNTGC 1 cut(s) 98
BstDEI CTNAG 2 cut(s) 54, 122
BstF5I GGATG 1 cut(s) 86
BstH2I RGCGCY 1 cut(s) 152
BstHHI GCGC 1 cut(s) 151
BstKTI GATC 1 cut(s) 219
BstMAI GTCTC 2 cut(s) 153, 305
BstMBI GATC 1 cut(s) 216
BstMWI GCNNNNNNNGC 1 cut(s) 98
BstNI CCWGG 1 cut(s) 297
BstNSI RCATGY 1 cut(s) 96
BstSCI CCNGG 2 cut(s) 76, 295
BstSFI CTRYAG 1 cut(s) 261
BstSLI GKGCMC 2 cut(s) 25, 121
BstV1I GCAGC 1 cut(s) 29
BstX2I RGATCY 1 cut(s) 216
BstYI RGATCY 1 cut(s) 216
BsuRI GGCC 1 cut(s) 240
BtsCI GGATG 1 cut(s) 86
BtsI GCAGTG 1 cut(s) 121
BtsIMutI CAGTG 2 cut(s) 23, 121
CfoI GCGC 1 cut(s) 151
Cfr13I GGNCC 1 cut(s) 239
CsiI ACCWGGT 1 cut(s) 295
Csp6I GTAC 1 cut(s) 208
CviAII CATG 1 cut(s) 93
CviJI RGCY 4 cut(s) 108, 240, 260, 272
CviKI_1 RGCY 4 cut(s) 108, 240, 260, 272
CviQI GTAC 1 cut(s) 208
DdeI CTNAG 2 cut(s) 54, 122
DpnI GATC 1 cut(s) 218
DpnII GATC 1 cut(s) 216
DriI GACNNNNNGTC 1 cut(s) 57
Eam1105I GACNNNNNGTC 1 cut(s) 57
Eco31I GGTCTC 1 cut(s) 305
Eco57I CTGAAG 1 cut(s) 125
EcoRII CCWGG 1 cut(s) 295
FaeI CATG 1 cut(s) 96
FaiI YATR 4 cut(s) 74, 94, 99, 135
FatI CATG 1 cut(s) 92
Fnu4HI GCNGC 1 cut(s) 43
FokI GGATG 1 cut(s) 93
Fsp4HI GCNGC 1 cut(s) 43
GlaI GCGC 1 cut(s) 150
GluI GCNGC 1 cut(s) 43
HaeII RGCGCY 1 cut(s) 152
HaeIII GGCC 1 cut(s) 240
HapII CCGG 2 cut(s) 77, 237
HhaI GCGC 1 cut(s) 151
Hin1II CATG 1 cut(s) 96
Hin6I GCGC 1 cut(s) 149
HinP1I GCGC 1 cut(s) 149
HinfI GANTC 3 cut(s) 4, 233, 253
HpaII CCGG 2 cut(s) 77, 237
Hpy188I TCNGA 1 cut(s) 232
Hpy188III TCNNGA 4 cut(s) 143, 203, 245, 283
HpyCH4III ACNGT 1 cut(s) 37
HpyCH4V TGCA 1 cut(s) 92
HpyF10VI GCNNNNNNNGC 1 cut(s) 98
HpyF3I CTNAG 2 cut(s) 54, 122
Hsp92II CATG 1 cut(s) 96
HspAI GCGC 1 cut(s) 149
Kzo9I GATC 1 cut(s) 216
LpnPI CCDG 9 cut(s) 11, 90, 128, 242, 250, 258, 263, 282, 296
Lsp1109I GCAGC 1 cut(s) 29
LweI GCATC 1 cut(s) 71
MabI ACCWGGT 1 cut(s) 295
MaeIII GTNAC 2 cut(s) 166, 265
MalI GATC 1 cut(s) 218
MboI GATC 1 cut(s) 216
MboII GAAGA 1 cut(s) 131
MflI RGATCY 1 cut(s) 216
MhlI GDGCHC 2 cut(s) 25, 121
MlyI GAGTC 1 cut(s) 247
MnlI CCTC 2 cut(s) 49, 252
MseI TTAA 1 cut(s) 30
MslI CAYNNNNRTG 1 cut(s) 189
MspI CCGG 2 cut(s) 77, 237
MspR9I CCNGG 2 cut(s) 78, 297
MvaI CCWGG 1 cut(s) 297
MwoI GCNNNNNNNGC 1 cut(s) 98
NciI CCSGG 1 cut(s) 78
NdeII GATC 1 cut(s) 216
NlaIII CATG 1 cut(s) 96
NlaIV GGNNCC 1 cut(s) 294
NmuCI GTSAC 1 cut(s) 166
NspI RCATGY 1 cut(s) 96
PfeI GAWTC 1 cut(s) 233
PflFI GACNNNGTC 1 cut(s) 164
PflMI CCANNNNNTGG 1 cut(s) 289
PkrI GCNGC 1 cut(s) 44
PleI GAGTC 1 cut(s) 247
PpsI GAGTC 1 cut(s) 247
Psp6I CCWGG 1 cut(s) 295
PspGI CCWGG 1 cut(s) 295
PspN4I GGNNCC 1 cut(s) 294
PspPI GGNCC 1 cut(s) 239
PsuI RGATCY 1 cut(s) 216
PsyI GACNNNGTC 1 cut(s) 164
RsaI GTAC 1 cut(s) 209
RsaNI GTAC 1 cut(s) 208
RseI CAYNNNNRTG 1 cut(s) 189
SaqAI TTAA 1 cut(s) 30
SatI GCNGC 1 cut(s) 43
Sau3AI GATC 1 cut(s) 216
Sau96I GGNCC 1 cut(s) 239
SchI GAGTC 1 cut(s) 247
ScrFI CCNGG 2 cut(s) 78, 297
SduI GDGCHC 2 cut(s) 25, 121
SetI ASST 2 cut(s) 252, 301
SexAI ACCWGGT 1 cut(s) 295
SfaNI GCATC 1 cut(s) 71
SfcI CTRYAG 1 cut(s) 261
SmiMI CAYNNNNRTG 1 cut(s) 189
SsiI CCGC 1 cut(s) 112
StyD4I CCNGG 2 cut(s) 76, 295
TaaI ACNGT 1 cut(s) 37
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 1 cut(s) 30
Tru9I TTAA 1 cut(s) 30
TscAI CASTG 2 cut(s) 30, 121
TseFI GTSAC 1 cut(s) 166
TseI GCWGC 1 cut(s) 42
Tsp45I GTSAC 1 cut(s) 166
TspGWI ACGGA 1 cut(s) 209
TspRI CASTG 2 cut(s) 30, 121
Tth111I GACNNNGTC 1 cut(s) 164
Van91I CCANNNNNTGG 1 cut(s) 289
XceI RCATGY 1 cut(s) 96
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.