FvH4_5g37140

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0006900
Physical Location & Seq
Reverse (-)
27219808 .. 27222759
2952 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g37140.t1

Sequence Viewer

Length: 711 bp
ATGTCAAACCACGCTGAAGATCTCTCTCAATTCGACATCTCCGTTCAGGAAAAGGACAAGCTCGTCGCTGAGGTCATCCGCTACGTGCTTTTCAAGACCCACCACAACTCAGGCTGCCCAATCCGGCGAGAAGAGCTGACCCAGTTGATCACCAAGAACTACCGGCAGCGGAATCTTCCGGTGATGATTATCAACGAGGCGATAGCGAAGCTCTCCACCATTTTCGGGTACGAAATGCGGGAGCTTCAGAGGGCCCGGCCCTCTTCGAATAACCACCAGGGCCGCAATTCGCAACAGAGTGCGGCTGAGGCGAAAACTTACGTTATCATGAGTAAGCTGCCGGCTGAGGTGTACACGAAGTATGTTGAGGATGAGGATACGGCTCATCTTACTGGTTTTACTTTTGTTGTGATGAGCATTGTGCATATTTCGGGTGGTAAAATATCCGAAGAGAATCTTTGGCAACATTTGAGGCGGATAGGATTGGATGAAGCCAACGAAAGCCATCCATTTTTAGGAAACACAAAACAAGCATTGGAGGGACTTGTCCAGCAGAGGTATTTGCAGAAGGACAAACAAAGTGGACCTGAAGGCAATACTTTGATATATGAGCTTGCTGAGAGAGCTTTAGATTCTCAAGTTACTGCAAGTTTGAAAGATTACATATCACAGATTGTGAATAAAGATGTTGGCTCGGTGGAGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

237

Amino Acids

26.85

Weight (kDa)

5.8

Isoelectric Point (pI)

48.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 25 - 210 3.3e-45 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 62
AciI CCGC 6 cut(s) 79, 169, 238, 283, 302, 475
AcuI CTGAAG 3 cut(s) 36, 230, 609
AfaI GTAC 2 cut(s) 230, 353
AfiI CCNNNNNNNGG 2 cut(s) 225, 515
AgsI TTSAA 2 cut(s) 94, 655
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 7 cut(s) 61, 136, 211, 244, 337, 613, 626
AluI AGCT 7 cut(s) 61, 136, 211, 244, 337, 613, 626
AoxI GGCC 3 cut(s) 252, 257, 280
ApaI GGGCCC 1 cut(s) 256
ApeKI GCWGC 3 cut(s) 114, 166, 337
AspS9I GGNCC 5 cut(s) 252, 253, 258, 280, 584
AsuC2I CCSGG 1 cut(s) 256
AsuHPI GGTGA 2 cut(s) 142, 193
AsuII TTCGAA 1 cut(s) 266
AvaII GGWCC 1 cut(s) 584
BaeGI GKGCMC 1 cut(s) 256
BanII GRGCYC 1 cut(s) 256
BbvCI CCTCAGC 3 cut(s) 69, 306, 345
BbvI GCAGC 3 cut(s) 101, 178, 324
BccI CCATC 1 cut(s) 513
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 278
BciVI GTATCC 1 cut(s) 370
BclI TGATCA 1 cut(s) 147
BcnI CCSGG 1 cut(s) 256
BfuI GTATCC 1 cut(s) 370
BglII AGATCT 1 cut(s) 19
BisI GCNGC 5 cut(s) 115, 167, 283, 303, 338
BlsI GCNGC 5 cut(s) 116, 168, 284, 304, 339
Bme1390I CCNGG 2 cut(s) 256, 278
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 5 cut(s) 252, 253, 258, 280, 584
BmiI GGNNCC 1 cut(s) 254
BmrFI CCNGG 2 cut(s) 256, 278
BmrI ACTGGG 1 cut(s) 136
BmuI ACTGGG 1 cut(s) 136
Bpu10I CCTNAGC 3 cut(s) 69, 306, 345
Bpu14I TTCGAA 1 cut(s) 266
BpuEI CTTGAG 1 cut(s) 621
BpuMI CCSGG 1 cut(s) 256
BsaAI YACGTR 1 cut(s) 85
BsaBI GATNNNNATC 1 cut(s) 188
BsaJI CCNNGG 1 cut(s) 277
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 1 cut(s) 692
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 515
Bse118I RCCGGY 2 cut(s) 162, 340
Bse1I ACTGG 2 cut(s) 142, 397
Bse8I GATNNNNATC 1 cut(s) 188
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 277
BseGI GGATG 5 cut(s) 75, 376, 493, 505, 709
BseJI GATNNNNATC 1 cut(s) 188
BseLI CCNNNNNNNGG 2 cut(s) 225, 515
BseMII CTCAG 5 cut(s) 60, 123, 297, 336, 609
BseNI ACTGG 2 cut(s) 142, 397
BseSI GKGCMC 1 cut(s) 256
BseXI GCAGC 3 cut(s) 101, 178, 324
BshFI GGCC 3 cut(s) 254, 259, 282
BsiSI CCGG 5 cut(s) 124, 163, 179, 256, 341
BslFI GGGAC 1 cut(s) 555
BslI CCNNNNNNNGG 2 cut(s) 225, 515
BsmFI GGGAC 1 cut(s) 555
BsnI GGCC 3 cut(s) 254, 259, 282
Bsp119I TTCGAA 1 cut(s) 266
Bsp120I GGGCCC 1 cut(s) 252
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 1 cut(s) 351
Bsp143I GATC 2 cut(s) 19, 147
BspACI CCGC 6 cut(s) 79, 169, 238, 283, 302, 475
BspANI GGCC 3 cut(s) 254, 259, 282
BspCNI CTCAG 5 cut(s) 61, 122, 298, 337, 610
BspHI TCATGA 1 cut(s) 327
BspLI GGNNCC 1 cut(s) 254
BspQI GCTCTTC 1 cut(s) 126
BspT104I TTCGAA 1 cut(s) 266
BsrFI RCCGGY 2 cut(s) 162, 340
BsrGI TGTACA 1 cut(s) 351
BsrI ACTGG 2 cut(s) 142, 397
BssAI RCCGGY 2 cut(s) 162, 340
BssECI CCNNGG 1 cut(s) 277
BssMI GATC 2 cut(s) 19, 147
Bst2UI CCWGG 1 cut(s) 278
Bst6I CTCTTC 3 cut(s) 126, 268, 444
BstAUI TGTACA 1 cut(s) 351
BstBAI YACGTR 1 cut(s) 85
BstBI TTCGAA 1 cut(s) 266
BstC8I GCNNGC 2 cut(s) 342, 615
BstDEI CTNAG 5 cut(s) 69, 109, 306, 345, 618
BstF5I GGATG 5 cut(s) 75, 376, 493, 505, 709
BstKTI GATC 2 cut(s) 22, 150
BstMBI GATC 2 cut(s) 19, 147
BstMWI GCNNNNNNNGC 3 cut(s) 133, 308, 623
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 2 cut(s) 254, 276
BstSLI GKGCMC 1 cut(s) 256
BstV1I GCAGC 3 cut(s) 101, 178, 324
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuI GTATCC 1 cut(s) 370
BsuRI GGCC 3 cut(s) 254, 259, 282
BtsCI GGATG 5 cut(s) 75, 376, 493, 505, 709
Cac8I GCNNGC 2 cut(s) 342, 615
CciI TCATGA 1 cut(s) 327
Cfr10I RCCGGY 2 cut(s) 162, 340
Cfr13I GGNCC 5 cut(s) 252, 253, 258, 280, 584
Csp6I GTAC 2 cut(s) 229, 352
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 1 cut(s) 328
CviQI GTAC 2 cut(s) 229, 352
DdeI CTNAG 5 cut(s) 69, 109, 306, 345, 618
DpnI GATC 2 cut(s) 21, 149
DpnII GATC 2 cut(s) 19, 147
DrdI GACNNNNNNGTC 1 cut(s) 62
DseDI GACNNNNNNGTC 1 cut(s) 62
Eam1104I CTCTTC 3 cut(s) 126, 268, 444
EarI CTCTTC 3 cut(s) 126, 268, 444
EciI GGCGGA 1 cut(s) 490
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 36, 230, 609
EcoO109I RGGNCCY 1 cut(s) 252
EcoRII CCWGG 1 cut(s) 276
EcoT38I GRGCYC 1 cut(s) 256
FaeI CATG 1 cut(s) 331
FaiI YATR 6 cut(s) 329, 363, 426, 607, 609, 665
FalI AAGNNNNNCTT 2 cut(s) 441, 473
FaqI GGGAC 1 cut(s) 555
FatI CATG 1 cut(s) 327
FauI CCCGC 1 cut(s) 231
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 5 cut(s) 115, 167, 283, 303, 338
FokI GGATG 4 cut(s) 62, 383, 492, 500
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 5 cut(s) 115, 167, 283, 303, 338
GluI GCNGC 5 cut(s) 115, 167, 283, 303, 338
HaeIII GGCC 3 cut(s) 254, 259, 282
HapII CCGG 5 cut(s) 124, 163, 179, 256, 341
Hin1II CATG 1 cut(s) 331
HinfI GANTC 3 cut(s) 172, 454, 632
HpaII CCGG 5 cut(s) 124, 163, 179, 256, 341
HphI GGTGA 2 cut(s) 142, 193
Hpy166II GTNNAC 3 cut(s) 352, 354, 584
Hpy188I TCNGA 2 cut(s) 249, 448
Hpy188III TCNNGA 3 cut(s) 47, 94, 328
Hpy8I GTNNAC 3 cut(s) 352, 354, 584
Hpy99I CGWCG 1 cut(s) 68
HpyAV CCTTC 2 cut(s) 562, 584
HpyCH4IV ACGT 2 cut(s) 84, 321
HpyCH4V TGCA 3 cut(s) 424, 565, 647
HpyF10VI GCNNNNNNNGC 3 cut(s) 133, 308, 623
HpyF3I CTNAG 5 cut(s) 69, 109, 306, 345, 618
HpySE526I ACGT 2 cut(s) 84, 321
Hsp92II CATG 1 cut(s) 331
KroI GCCGGC 1 cut(s) 340
KroNI GCCGGC 1 cut(s) 342
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 2 cut(s) 19, 147
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 3 cut(s) 101, 178, 324
MaeII ACGT 2 cut(s) 84, 321
MaeIII GTNAC 1 cut(s) 640
MalI GATC 2 cut(s) 21, 149
MboI GATC 2 cut(s) 19, 147
MboII GAAGA 5 cut(s) 29, 143, 167, 255, 461
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 2 cut(s) 29, 286
MroNI GCCGGC 1 cut(s) 340
MspA1I CMGCKG 1 cut(s) 169
MspI CCGG 5 cut(s) 124, 163, 179, 256, 341
MspR9I CCNGG 2 cut(s) 256, 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 3 cut(s) 133, 308, 623
NaeI GCCGGC 1 cut(s) 342
NciI CCSGG 1 cut(s) 256
NdeII GATC 2 cut(s) 19, 147
NgoMIV GCCGGC 1 cut(s) 340
NlaIII CATG 1 cut(s) 331
NlaIV GGNNCC 1 cut(s) 254
NspV TTCGAA 1 cut(s) 266
PagI TCATGA 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PdiI GCCGGC 1 cut(s) 342
PfeI GAWTC 3 cut(s) 172, 454, 632
PkrI GCNGC 5 cut(s) 116, 168, 284, 304, 339
Ppu21I YACGTR 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 254
PspOMI GGGCCC 1 cut(s) 252
PspPI GGNCC 5 cut(s) 252, 253, 258, 280, 584
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 2 cut(s) 230, 353
RsaNI GTAC 2 cut(s) 229, 352
SapI GCTCTTC 1 cut(s) 126
SatI GCNGC 5 cut(s) 115, 167, 283, 303, 338
Sau3AI GATC 2 cut(s) 19, 147
Sau96I GGNCC 5 cut(s) 252, 253, 258, 280, 584
ScrFI CCNGG 2 cut(s) 256, 278
SduI GDGCHC 1 cut(s) 256
SfuI TTCGAA 1 cut(s) 266
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
Sse9I AATT 2 cut(s) 29, 286
SsiI CCGC 6 cut(s) 79, 169, 238, 283, 302, 475
StyD4I CCNGG 2 cut(s) 254, 276
TaiI ACGT 2 cut(s) 87, 324
TaqI TCGA 2 cut(s) 33, 266
TasI AATT 2 cut(s) 29, 286
TatI WGTACW 1 cut(s) 351
TauI GCSGC 2 cut(s) 285, 305
TfiI GAWTC 3 cut(s) 172, 454, 632
TseI GCWGC 3 cut(s) 114, 166, 337
TspDTI ATGAA 1 cut(s) 504
TspGWI ACGGA 1 cut(s) 31
VpaK11BI GGWCC 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.