MD08G1184100.v1.1

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr08
OG0006900
Physical Location & Seq
Forward (+)
22938046 .. 22941544
3499 bp
Loading structure...
UTR
Exon/CDS
Intron
MD08G1184100.v1.1.491

Sequence Viewer

Length: 717 bp
ATGTCACAACCAGTTGTAGATCTCTCTCAATTCGACATATCCAAAGAGGAAAAGGACAAGCTTGTAGCGGAAGTAATCCGATACGTGCTGTTCAAGACGCACCACAGCTCGGGGTGTCCGATAAAGAGGGAAGAGCTCACCCAGCTGCTCACCAAGAACTACCGCCAGCGCAATCTTCCTACTTTCATCATCAACGAGGCCGTACAGAAGTTGTCCTCCATTTTCGGGTACGAAATGCGGGAGCTTCAGAGGTCTCGTCCTTCTTCCGCTAATCAGGGACGCATTTCGCAACAGAGTGCTGCGGAGGCAAAATCCTATATCATCACGAGTAAGCTACCTTCTGATGTGTATAAGAAGTATGTCTTGAACGACAATGATAGTACGGTTCCTCTCAATGGTTTCACTTTTGTCGTACTGAGTCTTGTACATATTTCCGGCGGTAAAATGACTGAAGAGGATCTTTGGCGTAATTTGAGACGAATGGGATTGGACGAAAGCAATGAAAACCATCCAGTCCTAGGAAACATAAAGCAAGCACTGGACACACTTGTCCAGCAAAGATATTTGCAGAAAGACAAAGTAAGTGGACCTGAAGGCAATACTTTGTTTTATGAGCTTGCGGAGAGAGCTTTAGATGCACCAATTAGTGAGAGCACAAAAGCACACATATCAGAGATTGTGAACAAAGAGGTTGTCTCCGTGGATGTCGACGACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

239

Amino Acids

26.99

Weight (kDa)

6.01

Isoelectric Point (pI)

52.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 25 - 211 4.4e-45 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 548
AccI GTMKAC 1 cut(s) 708
AciI CCGC 7 cut(s) 68, 163, 238, 267, 302, 438, 620
AclWI GGATC 1 cut(s) 465
AcuI CTGAAG 3 cut(s) 230, 471, 612
AfaI GTAC 5 cut(s) 204, 230, 382, 414, 426
AfiI CCNNNNNNNGG 4 cut(s) 109, 225, 395, 518
AgsI TTSAA 2 cut(s) 94, 367
AluBI AGCT 8 cut(s) 61, 108, 136, 145, 244, 334, 616, 629
AluI AGCT 8 cut(s) 61, 108, 136, 145, 244, 334, 616, 629
Alw21I GWGCWC 2 cut(s) 138, 656
Alw26I GTCTC 3 cut(s) 258, 469, 700
AlwI GGATC 1 cut(s) 465
Ama87I CYCGRG 1 cut(s) 109
AoxI GGCC 1 cut(s) 198
ApeKI GCWGC 2 cut(s) 145, 299
ArsI GACNNNNNNTTYG 2 cut(s) 678, 710
AspA2I CCTAGG 1 cut(s) 517
AspLEI GCGC 1 cut(s) 171
AspS9I GGNCC 1 cut(s) 587
AsuHPI GGTGA 2 cut(s) 130, 142
AvaI CYCGRG 1 cut(s) 109
AvaII GGWCC 1 cut(s) 587
AvrII CCTAGG 1 cut(s) 517
BanII GRGCYC 1 cut(s) 138
BarI GAAGNNNNNNTAC 2 cut(s) 322, 354
BauI CACGAG 1 cut(s) 325
Bbv12I GWGCWC 2 cut(s) 138, 656
BbvI GCAGC 2 cut(s) 132, 286
BccI CCATC 1 cut(s) 516
BceAI ACGGC 1 cut(s) 185
BcoDI GTCTC 3 cut(s) 258, 469, 700
BfaI CTAG 2 cut(s) 518, 715
BglII AGATCT 1 cut(s) 19
BisI GCNGC 2 cut(s) 146, 300
BlnI CCTAGG 1 cut(s) 517
BlsI GCNGC 2 cut(s) 147, 301
Bme18I GGWCC 1 cut(s) 587
BmeT110I CYCGRG 1 cut(s) 109
BmgT120I GGNCC 1 cut(s) 587
BmiI GGNNCC 1 cut(s) 387
BmsI GCATC 1 cut(s) 625
BplI GAGNNNNNCTC 2 cut(s) 680, 712
BsaAI YACGTR 1 cut(s) 85
BsaI GGTCTC 1 cut(s) 258
BsaJI CCNNGG 2 cut(s) 517, 699
Bsc4I CCNNNNNNNGG 4 cut(s) 109, 225, 395, 518
Bse1I ACTGG 3 cut(s) 11, 512, 543
Bse3DI GCAATG 1 cut(s) 505
BseDI CCNNGG 2 cut(s) 517, 699
BseGI GGATG 2 cut(s) 508, 709
BseLI CCNNNNNNNGG 4 cut(s) 109, 225, 395, 518
BseMI GCAATG 1 cut(s) 505
BseMII CTCAG 1 cut(s) 407
BseNI ACTGG 3 cut(s) 11, 512, 543
BseXI GCAGC 2 cut(s) 132, 286
BseYI CCCAGC 1 cut(s) 141
BshFI GGCC 1 cut(s) 200
BsiHKAI GWGCWC 2 cut(s) 138, 656
BsiHKCI CYCGRG 1 cut(s) 109
BsiSI CCGG 1 cut(s) 435
BslFI GGGAC 1 cut(s) 291
BslI CCNNNNNNNGG 4 cut(s) 109, 225, 395, 518
BsmAI GTCTC 3 cut(s) 258, 469, 700
BsmBI CGTCTC 1 cut(s) 469
BsmFI GGGAC 1 cut(s) 291
BsnI GGCC 1 cut(s) 200
Bso31I GGTCTC 1 cut(s) 258
BsoBI CYCGRG 1 cut(s) 109
Bsp1286I GDGCHC 2 cut(s) 138, 656
Bsp1407I TGTACA 1 cut(s) 424
Bsp143I GATC 2 cut(s) 19, 457
BspACI CCGC 7 cut(s) 68, 163, 238, 267, 302, 438, 620
BspANI GGCC 1 cut(s) 200
BspCNI CTCAG 1 cut(s) 408
BspLI GGNNCC 1 cut(s) 387
BspPI GGATC 1 cut(s) 465
BspQI GCTCTTC 1 cut(s) 126
BspTNI GGTCTC 1 cut(s) 258
BsrDI GCAATG 1 cut(s) 505
BsrGI TGTACA 1 cut(s) 424
BsrI ACTGG 3 cut(s) 11, 512, 543
BssECI CCNNGG 2 cut(s) 517, 699
BssMI GATC 2 cut(s) 19, 457
BssSI CACGAG 1 cut(s) 325
BssT1I CCWWGG 1 cut(s) 517
Bst2BI CACGAG 1 cut(s) 325
Bst4CI ACNGT 1 cut(s) 385
Bst6I CTCTTC 2 cut(s) 126, 447
BstAUI TGTACA 1 cut(s) 424
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 167, 534, 618
BstDEI CTNAG 1 cut(s) 416
BstDSI CCRYGG 1 cut(s) 699
BstF5I GGATG 2 cut(s) 508, 709
BstHHI GCGC 1 cut(s) 171
BstKTI GATC 2 cut(s) 22, 460
BstMAI GTCTC 3 cut(s) 258, 469, 700
BstMBI GATC 2 cut(s) 19, 457
BstMWI GCNNNNNNNGC 4 cut(s) 142, 305, 626, 635
BstV1I GCAGC 2 cut(s) 132, 286
BstX2I RGATCY 2 cut(s) 19, 457
BstYI RGATCY 2 cut(s) 19, 457
BsuRI GGCC 1 cut(s) 200
BtgI CCRYGG 1 cut(s) 699
BtsCI GGATG 2 cut(s) 508, 709
BtsIMutI CAGTG 1 cut(s) 536
Cac8I GCNNGC 3 cut(s) 167, 534, 618
CfoI GCGC 1 cut(s) 171
Cfr13I GGNCC 1 cut(s) 587
CseI GACGC 2 cut(s) 106, 288
Csp6I GTAC 5 cut(s) 203, 229, 381, 413, 425
CspCI CAANNNNNGTGG 2 cut(s) 565, 600
CviJI RGCY 9 cut(s) 61, 108, 136, 145, 200, 244, 334, 616, 629
CviKI_1 RGCY 9 cut(s) 61, 108, 136, 145, 200, 244, 334, 616, 629
CviQI GTAC 5 cut(s) 203, 229, 381, 413, 425
DdeI CTNAG 1 cut(s) 416
DpnI GATC 2 cut(s) 21, 459
DpnII GATC 2 cut(s) 19, 457
DrdI GACNNNNNNGTC 1 cut(s) 548
DseDI GACNNNNNNGTC 1 cut(s) 548
Eam1104I CTCTTC 2 cut(s) 126, 447
EarI CTCTTC 2 cut(s) 126, 447
Ecl136II GAGCTC 1 cut(s) 136
Eco130I CCWWGG 1 cut(s) 517
Eco24I GRGCYC 1 cut(s) 138
Eco31I GGTCTC 1 cut(s) 258
Eco47I GGWCC 1 cut(s) 587
Eco53kI GAGCTC 1 cut(s) 136
Eco57I CTGAAG 3 cut(s) 230, 471, 612
Eco88I CYCGRG 1 cut(s) 109
EcoICRI GAGCTC 1 cut(s) 136
EcoT14I CCWWGG 1 cut(s) 517
EcoT38I GRGCYC 1 cut(s) 138
ErhI CCWWGG 1 cut(s) 517
Esp3I CGTCTC 1 cut(s) 469
FaiI YATR 8 cut(s) 38, 318, 351, 360, 429, 527, 612, 668
FalI AAGNNNNNCTT 4 cut(s) 347, 379, 444, 476
FaqI GGGAC 1 cut(s) 291
FauI CCCGC 1 cut(s) 231
FblI GTMKAC 1 cut(s) 708
Fnu4HI GCNGC 2 cut(s) 146, 300
FokI GGATG 1 cut(s) 495
FriOI GRGCYC 1 cut(s) 138
Fsp4HI GCNGC 2 cut(s) 146, 300
FspBI CTAG 2 cut(s) 518, 715
GlaI GCGC 1 cut(s) 170
GluI GCNGC 2 cut(s) 146, 300
GsaI CCCAGC 1 cut(s) 145
HaeIII GGCC 1 cut(s) 200
HapII CCGG 1 cut(s) 435
HgaI GACGC 2 cut(s) 106, 288
HhaI GCGC 1 cut(s) 171
Hin6I GCGC 1 cut(s) 169
HinP1I GCGC 1 cut(s) 169
HincII GTYRAC 1 cut(s) 709
HindII GTYRAC 1 cut(s) 709
HindIII AAGCTT 1 cut(s) 59
HinfI GANTC 1 cut(s) 418
HpaII CCGG 1 cut(s) 435
HphI GGTGA 2 cut(s) 130, 142
Hpy166II GTNNAC 3 cut(s) 587, 682, 709
Hpy188I TCNGA 5 cut(s) 80, 120, 249, 343, 673
Hpy188III TCNNGA 3 cut(s) 94, 325, 364
Hpy8I GTNNAC 3 cut(s) 587, 682, 709
Hpy99I CGWCG 1 cut(s) 713
HpyAV CCTTC 3 cut(s) 270, 348, 587
HpyCH4III ACNGT 1 cut(s) 385
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 2 cut(s) 568, 638
HpyF10VI GCNNNNNNNGC 4 cut(s) 142, 305, 626, 635
HpyF3I CTNAG 1 cut(s) 416
HpySE526I ACGT 1 cut(s) 84
HspAI GCGC 1 cut(s) 169
Kzo9I GATC 2 cut(s) 19, 457
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 241
LpnPI CCDG 9 cut(s) 24, 155, 179, 260, 448, 524, 525, 566, 603
Lsp1109I GCAGC 2 cut(s) 132, 286
LweI GCATC 1 cut(s) 625
MaeI CTAG 2 cut(s) 518, 715
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 21, 459
MboI GATC 2 cut(s) 19, 457
MboII GAAGA 4 cut(s) 143, 167, 255, 464
MflI RGATCY 2 cut(s) 19, 457
MhlI GDGCHC 2 cut(s) 138, 656
MluCI AATT 3 cut(s) 29, 469, 642
MlyI GAGTC 1 cut(s) 427
MnlI CCTC 9 cut(s) 40, 120, 190, 226, 243, 298, 399, 448, 682
MspA1I CMGCKG 1 cut(s) 145
MspI CCGG 1 cut(s) 435
MwoI GCNNNNNNNGC 4 cut(s) 142, 305, 626, 635
NdeII GATC 2 cut(s) 19, 457
NlaIV GGNNCC 1 cut(s) 387
NmuCI GTSAC 1 cut(s) 3
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 116
PkrI GCNGC 2 cut(s) 147, 301
PleI GAGTC 1 cut(s) 426
PpsI GAGTC 1 cut(s) 426
Ppu21I YACGTR 1 cut(s) 85
Psp124BI GAGCTC 1 cut(s) 138
PspFI CCCAGC 1 cut(s) 141
PspN4I GGNNCC 1 cut(s) 387
PspPI GGNCC 1 cut(s) 587
PsuI RGATCY 2 cut(s) 19, 457
PvuII CAGCTG 1 cut(s) 145
RsaI GTAC 5 cut(s) 204, 230, 382, 414, 426
RsaNI GTAC 5 cut(s) 203, 229, 381, 413, 425
SacI GAGCTC 1 cut(s) 138
SalI GTCGAC 1 cut(s) 707
SapI GCTCTTC 1 cut(s) 126
SatI GCNGC 2 cut(s) 146, 300
Sau3AI GATC 2 cut(s) 19, 457
Sau96I GGNCC 1 cut(s) 587
SchI GAGTC 1 cut(s) 427
SduI GDGCHC 2 cut(s) 138, 656
SfaNI GCATC 1 cut(s) 625
SinI GGWCC 1 cut(s) 587
Sse9I AATT 3 cut(s) 29, 469, 642
SsiI CCGC 7 cut(s) 68, 163, 238, 267, 302, 438, 620
SspMI CTAG 2 cut(s) 518, 715
SstI GAGCTC 1 cut(s) 138
StyI CCWWGG 1 cut(s) 517
TaaI ACNGT 1 cut(s) 385
TaiI ACGT 1 cut(s) 87
TaqI TCGA 2 cut(s) 33, 708
TasI AATT 3 cut(s) 29, 469, 642
TatI WGTACW 1 cut(s) 424
TscAI CASTG 1 cut(s) 543
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 2 cut(s) 145, 299
Tsp45I GTSAC 1 cut(s) 3
TspDTI ATGAA 2 cut(s) 175, 516
TspGWI ACGGA 1 cut(s) 688
TspRI CASTG 1 cut(s) 543
VpaK11BI GGWCC 1 cut(s) 587
XmaJI CCTAGG 1 cut(s) 517
XmiI GTMKAC 1 cut(s) 708
XspI CTAG 2 cut(s) 518, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.