Rw7G039850

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr7
OG0006900
Physical Location & Seq
Reverse (-)
61529086 .. 61532191
3106 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw7G039850.1

Sequence Viewer

Length: 837 bp
ATGTCAAACCATGGTGAAGACCTCTCTCAATTCGATATCTCCGTTCAGGTACGCACGCACCCGATTTCATACTCCAAATCTCAATCTCATCGACCCAAAGCTTTCAATTTTAACAGCGGTGACCTCCTCCGATTTCCGATCTCGTCTTCTTCTTTTTTTTTCTTTCTTTCTCAGGAAAAGGACAAGCTCGTAGCGGAAGTAATCCGCTACGTGCTTTTCAAGACGCACCACAACTCCGGCTGCCCAATTAGGAGAGAAGAGCTGACTCAGTTGATCACCAAAAACTACCGCCAGCGAAATCTTCCGGTGCTAATTATCAACGAGGCAATAAACAAGCTCTCCACCATTTTCGGGTACGAAATGAGGGAGCTTCAGAGGGCTCGTCCCTCCTCCAATAACCATCAGGGCCGCAATTCTCAACAAGGCGCGGCAGAGGCGAAAACGTATGTTATCATGAGTAAGCTGCCTGCTGAGGTGTACAAGAAGTATGTTGAGGATGTTAATACGGCTCATCTTACTGGATTTACTTTCGTTGTACTGAGTATTGTACATATTTCAGGTGGTAAAATCTCCGAAGAGAATCTGTGGCAACATTTGAGGCGGATAGGACTGGATGAAACTAATGAAAGCCATCCAGTTCTAGGAAACACGAAACAAGCACTGGAGACACTTGTCCAGCAAAGGTATTTGCAGAAGGACAAACAAAGTGGACCTGAAGGCAATACTTTGTTATATGAGCTTGCTGAGAGAGCTTTAGATTCTCAAGTTACTGCAACCATGAAAGATTACATATCGCAGATTGTGAATAAAGATGTTGGCTCAGTGGAGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

278

Amino Acids

31.72

Weight (kDa)

7.86

Isoelectric Point (pI)

44.9

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 67 - 252 2.1e-45 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 428
AciI CCGC 7 cut(s) 117, 194, 205, 289, 409, 428, 601
AcuI CTGAAG 2 cut(s) 356, 735
AfaI GTAC 5 cut(s) 51, 356, 479, 537, 549
AfiI CCNNNNNNNGG 2 cut(s) 351, 641
AgsI TTSAA 2 cut(s) 106, 220
AluBI AGCT 8 cut(s) 101, 187, 262, 337, 370, 463, 739, 752
AluI AGCT 8 cut(s) 101, 187, 262, 337, 370, 463, 739, 752
Alw26I GTCTC 1 cut(s) 659
AoxI GGCC 1 cut(s) 406
ApeKI GCWGC 2 cut(s) 240, 463
AspLEI GCGC 1 cut(s) 428
AspS9I GGNCC 2 cut(s) 406, 710
AsuHPI GGTGA 3 cut(s) 26, 131, 268
AvaII GGWCC 1 cut(s) 710
BanII GRGCYC 1 cut(s) 382
BbsI GAAGAC 2 cut(s) 24, 138
BbvCI CCTCAGC 1 cut(s) 471
BbvI GCAGC 2 cut(s) 227, 450
BccI CCATC 2 cut(s) 408, 639
BceAI ACGGC 1 cut(s) 522
BclI TGATCA 1 cut(s) 273
BcoDI GTCTC 1 cut(s) 659
BfaI CTAG 1 cut(s) 641
BisI GCNGC 4 cut(s) 241, 409, 429, 464
BlsI GCNGC 4 cut(s) 242, 410, 430, 465
Bme18I GGWCC 1 cut(s) 710
BmgT120I GGNCC 2 cut(s) 406, 710
BoxI GACNNNNGTC 1 cut(s) 671
BpiI GAAGAC 2 cut(s) 24, 138
BpmI CTGGAG 1 cut(s) 683
Bpu10I CCTNAGC 1 cut(s) 471
BpuEI CTTGAG 1 cut(s) 747
BsaAI YACGTR 1 cut(s) 211
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 5 cut(s) 218, 248, 323, 353, 818
Bsc4I CCNNNNNNNGG 2 cut(s) 351, 641
Bse1I ACTGG 4 cut(s) 523, 615, 635, 666
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 4 cut(s) 502, 619, 631, 835
BseLI CCNNNNNNNGG 2 cut(s) 351, 641
BseMII CTCAG 6 cut(s) 185, 281, 462, 530, 735, 834
BseNI ACTGG 4 cut(s) 523, 615, 635, 666
BseRI GAGGAG 2 cut(s) 116, 379
BseXI GCAGC 2 cut(s) 227, 450
Bsh1236I CGCG 1 cut(s) 428
BshFI GGCC 1 cut(s) 408
BsiSI CCGG 2 cut(s) 237, 305
BslFI GGGAC 1 cut(s) 369
BslI CCNNNNNNNGG 2 cut(s) 351, 641
BsmAI GTCTC 1 cut(s) 659
BsmFI GGGAC 1 cut(s) 369
BsnI GGCC 1 cut(s) 408
Bsp1286I GDGCHC 1 cut(s) 382
Bsp1407I TGTACA 2 cut(s) 477, 547
Bsp143I GATC 2 cut(s) 138, 273
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 7 cut(s) 117, 194, 205, 289, 409, 428, 601
BspANI GGCC 1 cut(s) 408
BspCNI CTCAG 6 cut(s) 184, 280, 463, 531, 736, 833
BspFNI CGCG 1 cut(s) 428
BspHI TCATGA 1 cut(s) 453
BspQI GCTCTTC 1 cut(s) 252
BsrGI TGTACA 2 cut(s) 477, 547
BsrI ACTGG 4 cut(s) 523, 615, 635, 666
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 138, 273
BssT1I CCWWGG 1 cut(s) 10
Bst6I CTCTTC 2 cut(s) 252, 570
BstAUI TGTACA 2 cut(s) 477, 547
BstBAI YACGTR 1 cut(s) 211
BstC8I GCNNGC 4 cut(s) 56, 293, 468, 741
BstDEI CTNAG 6 cut(s) 171, 267, 471, 539, 744, 820
BstDSI CCRYGG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 4 cut(s) 502, 619, 631, 835
BstFNI CGCG 1 cut(s) 428
BstHHI GCGC 1 cut(s) 428
BstKTI GATC 2 cut(s) 141, 276
BstMAI GTCTC 1 cut(s) 659
BstMBI GATC 2 cut(s) 138, 273
BstMWI GCNNNNNNNGC 2 cut(s) 434, 749
BstPAI GACNNNNGTC 1 cut(s) 671
BstPI GGTNACC 1 cut(s) 119
BstUI CGCG 1 cut(s) 428
BstV1I GCAGC 2 cut(s) 227, 450
BstV2I GAAGAC 2 cut(s) 24, 138
BsuRI GGCC 1 cut(s) 408
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 4 cut(s) 502, 619, 631, 835
BtsIMutI CAGTG 2 cut(s) 659, 828
Cac8I GCNNGC 4 cut(s) 56, 293, 468, 741
CciI TCATGA 1 cut(s) 453
CfoI GCGC 1 cut(s) 428
Cfr13I GGNCC 2 cut(s) 406, 710
CseI GACGC 1 cut(s) 232
Csp6I GTAC 5 cut(s) 50, 355, 478, 536, 548
CspCI CAANNNNNGTGG 2 cut(s) 688, 723
CviAII CATG 3 cut(s) 11, 454, 778
CviQI GTAC 5 cut(s) 50, 355, 478, 536, 548
DdeI CTNAG 6 cut(s) 171, 267, 471, 539, 744, 820
DpnI GATC 2 cut(s) 140, 275
DpnII GATC 2 cut(s) 138, 273
Eam1104I CTCTTC 2 cut(s) 252, 570
EarI CTCTTC 2 cut(s) 252, 570
EciI GGCGGA 1 cut(s) 616
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 382
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 710
Eco57I CTGAAG 2 cut(s) 356, 735
Eco91I GGTNACC 1 cut(s) 119
EcoO65I GGTNACC 1 cut(s) 119
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 382
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 3 cut(s) 14, 457, 781
FaqI GGGAC 1 cut(s) 369
FatI CATG 3 cut(s) 10, 453, 777
FbaI TGATCA 1 cut(s) 273
Fnu4HI GCNGC 4 cut(s) 241, 409, 429, 464
FokI GGATG 3 cut(s) 509, 618, 626
FriOI GRGCYC 1 cut(s) 382
Fsp4HI GCNGC 4 cut(s) 241, 409, 429, 464
FspBI CTAG 1 cut(s) 641
GlaI GCGC 1 cut(s) 427
GluI GCNGC 4 cut(s) 241, 409, 429, 464
GsuI CTGGAG 1 cut(s) 683
HaeIII GGCC 1 cut(s) 408
HapII CCGG 2 cut(s) 237, 305
HgaI GACGC 1 cut(s) 232
HhaI GCGC 1 cut(s) 428
Hin1II CATG 3 cut(s) 14, 457, 781
Hin6I GCGC 1 cut(s) 426
HinP1I GCGC 1 cut(s) 426
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 3 cut(s) 265, 580, 758
HpaII CCGG 2 cut(s) 237, 305
HphI GGTGA 3 cut(s) 26, 131, 268
Hpy166II GTNNAC 2 cut(s) 478, 710
Hpy188I TCNGA 4 cut(s) 131, 138, 375, 574
Hpy188III TCNNGA 3 cut(s) 173, 220, 454
Hpy8I GTNNAC 2 cut(s) 478, 710
HpyAV CCTTC 2 cut(s) 688, 710
HpyCH4IV ACGT 2 cut(s) 210, 443
HpyCH4V TGCA 2 cut(s) 691, 773
HpyF10VI GCNNNNNNNGC 2 cut(s) 434, 749
HpyF3I CTNAG 6 cut(s) 171, 267, 471, 539, 744, 820
HpySE526I ACGT 2 cut(s) 210, 443
Hsp92II CATG 3 cut(s) 14, 457, 781
HspAI GCGC 1 cut(s) 426
Ksp22I TGATCA 1 cut(s) 273
Kzo9I GATC 2 cut(s) 138, 273
LguI GCTCTTC 1 cut(s) 252
LmnI GCTCC 1 cut(s) 367
Lsp1109I GCAGC 2 cut(s) 227, 450
MaeI CTAG 1 cut(s) 641
MaeII ACGT 2 cut(s) 210, 443
MaeIII GTNAC 2 cut(s) 119, 766
MalI GATC 2 cut(s) 140, 275
MboI GATC 2 cut(s) 138, 273
MboII GAAGA 6 cut(s) 29, 138, 141, 269, 293, 587
MhlI GDGCHC 1 cut(s) 382
MluCI AATT 5 cut(s) 29, 106, 246, 312, 412
MlyI GAGTC 1 cut(s) 259
MseI TTAA 2 cut(s) 111, 501
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 2 cut(s) 237, 305
MvnI CGCG 1 cut(s) 428
MwoI GCNNNNNNNGC 2 cut(s) 434, 749
NcoI CCATGG 1 cut(s) 10
NdeII GATC 2 cut(s) 138, 273
NlaIII CATG 3 cut(s) 14, 457, 781
NmuCI GTSAC 1 cut(s) 119
PagI TCATGA 1 cut(s) 453
PciSI GCTCTTC 1 cut(s) 252
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 2 cut(s) 580, 758
PkrI GCNGC 4 cut(s) 242, 410, 430, 465
PleI GAGTC 1 cut(s) 259
PpsI GAGTC 1 cut(s) 259
Ppu21I YACGTR 1 cut(s) 211
PshAI GACNNNNGTC 1 cut(s) 671
PspEI GGTNACC 1 cut(s) 119
PspPI GGNCC 2 cut(s) 406, 710
RsaI GTAC 5 cut(s) 51, 356, 479, 537, 549
RsaNI GTAC 5 cut(s) 50, 355, 478, 536, 548
SapI GCTCTTC 1 cut(s) 252
SaqAI TTAA 2 cut(s) 111, 501
SatI GCNGC 4 cut(s) 241, 409, 429, 464
Sau3AI GATC 2 cut(s) 138, 273
Sau96I GGNCC 2 cut(s) 406, 710
SchI GAGTC 1 cut(s) 259
SduI GDGCHC 1 cut(s) 382
SinI GGWCC 1 cut(s) 710
SmlI CTYRAG 1 cut(s) 762
SmoI CTYRAG 1 cut(s) 762
Sse9I AATT 5 cut(s) 29, 106, 246, 312, 412
SsiI CCGC 7 cut(s) 117, 194, 205, 289, 409, 428, 601
SspMI CTAG 1 cut(s) 641
StyI CCWWGG 1 cut(s) 10
TaiI ACGT 2 cut(s) 213, 446
TaqI TCGA 2 cut(s) 33, 91
TasI AATT 5 cut(s) 29, 106, 246, 312, 412
TatI WGTACW 3 cut(s) 477, 535, 547
TauI GCSGC 2 cut(s) 411, 431
TfiI GAWTC 2 cut(s) 580, 758
Tru1I TTAA 2 cut(s) 111, 501
Tru9I TTAA 2 cut(s) 111, 501
TscAI CASTG 2 cut(s) 666, 828
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 240, 463
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 4 cut(s) 57, 630, 639, 794
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 2 cut(s) 666, 828
VpaK11BI GGWCC 1 cut(s) 710
XspI CTAG 1 cut(s) 641
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.