RchiOBHm_Chr7g0243981

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
7
OG0006900
Physical Location & Seq
Reverse (-)
70277798 .. 70279078
1281 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ21867

Sequence Viewer

Length: 711 bp
ATGTCAAACCATGGTGAAGACCTCTCTCAATTCGATATCTCCGTTCAGGTACGCACGCACCCGATTTCATACTCCAAATCTCAATCTCATCGACCCAAAGCTTTCAATTTCAACAGCGGTGACCTCCTCCGATTTCCGATCTCGTCTTCTTTTTTTCTTTCTCAGGAAAAGGACAAGCTCGTAGCGGAAGTAATCCGCTACGTGCTTTTCAAGACGCACCACAACTCCGGCTGCCCAATTAGGAGAGAAGAGCTGACTCAGTTGATCACCAAAAACTACCGCCAGCGAAATCTTCCGGTGCTAATTATCAACGAGGCAATAAACAAGCTCTCCACCATTTTCGGGTACGAAATGAGGGAGCTTCAGAGGGCTCGTCCCTCCTCCAATAACCATCAGGGCCGCAATTCTCAACAAGTAAGCTGCCTGCTGAGGTACAAGAAGTATGTTGAGGATGTTAATACGGCTCATCTTACTGGATTTACTTTCGTTGTACTGAGTATTGTGCATATTTCAGGTGGTAAAATCTCCGAAGAGAATCTGTGGCAACATTTGAGGCGGATAGGACTGGATGAAACTAACGAAAGCCATCCAGTTCTAGGAAACACGAAACAAGCACTGGAGGCACTTGTCCAGCAAAGGTATTTGCAGAAGGACAAACAAAGTGACCTGAAGGCAATACTTTGTTATATGAGCTTGCTGAGAGAGCTTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

236

Amino Acids

27.3

Weight (kDa)

9.44

Isoelectric Point (pI)

52.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 64 - 221 2.2e-33 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
Loading...
Style Settings
Image
Tree File
Tip: Beautify your tree with professional tools

Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.

Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 6 cut(s) 117, 185, 196, 280, 400, 556
AcuI CTGAAG 2 cut(s) 347, 689
AfaI GTAC 4 cut(s) 51, 347, 434, 492
AfiI CCNNNNNNNGG 2 cut(s) 342, 596
AgsI TTSAA 3 cut(s) 106, 112, 211
AluBI AGCT 8 cut(s) 101, 178, 253, 328, 361, 420, 693, 706
AluI AGCT 8 cut(s) 101, 178, 253, 328, 361, 420, 693, 706
AoxI GGCC 1 cut(s) 397
ApeKI GCWGC 2 cut(s) 231, 420
AspS9I GGNCC 1 cut(s) 397
AsuHPI GGTGA 3 cut(s) 26, 131, 259
BanII GRGCYC 1 cut(s) 373
BbsI GAAGAC 2 cut(s) 24, 138
BbvCI CCTCAGC 1 cut(s) 428
BbvI GCAGC 2 cut(s) 218, 407
BccI CCATC 2 cut(s) 399, 594
BceAI ACGGC 1 cut(s) 477
BclI TGATCA 1 cut(s) 264
BfaI CTAG 1 cut(s) 596
BisI GCNGC 3 cut(s) 232, 400, 421
BlsI GCNGC 3 cut(s) 233, 401, 422
BmgT120I GGNCC 1 cut(s) 397
BpiI GAAGAC 2 cut(s) 24, 138
BpmI CTGGAG 1 cut(s) 638
Bpu10I CCTNAGC 1 cut(s) 428
BsaAI YACGTR 1 cut(s) 202
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 295
BsaXI ACNNNNNCTCC 4 cut(s) 209, 239, 314, 344
Bsc4I CCNNNNNNNGG 2 cut(s) 342, 596
Bse1I ACTGG 4 cut(s) 478, 570, 590, 621
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 3 cut(s) 457, 574, 586
BseLI CCNNNNNNNGG 2 cut(s) 342, 596
BseMII CTCAG 5 cut(s) 176, 272, 419, 485, 689
BseNI ACTGG 4 cut(s) 478, 570, 590, 621
BseRI GAGGAG 2 cut(s) 116, 370
BseXI GCAGC 2 cut(s) 218, 407
BshFI GGCC 1 cut(s) 399
BsiSI CCGG 2 cut(s) 228, 296
BslFI GGGAC 1 cut(s) 360
BslI CCNNNNNNNGG 2 cut(s) 342, 596
BsmFI GGGAC 1 cut(s) 360
BsnI GGCC 1 cut(s) 399
Bsp1286I GDGCHC 1 cut(s) 373
Bsp143I GATC 2 cut(s) 138, 264
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 6 cut(s) 117, 185, 196, 280, 400, 556
BspANI GGCC 1 cut(s) 399
BspCNI CTCAG 5 cut(s) 175, 271, 420, 486, 690
BspQI GCTCTTC 1 cut(s) 243
BsrI ACTGG 4 cut(s) 478, 570, 590, 621
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 138, 264
BssT1I CCWWGG 1 cut(s) 10
Bst6I CTCTTC 2 cut(s) 243, 525
BstBAI YACGTR 1 cut(s) 202
BstC8I GCNNGC 4 cut(s) 56, 284, 425, 695
BstDEI CTNAG 5 cut(s) 162, 258, 428, 494, 698
BstDSI CCRYGG 1 cut(s) 10
BstEII GGTNACC 1 cut(s) 119
BstF5I GGATG 3 cut(s) 457, 574, 586
BstKTI GATC 2 cut(s) 141, 267
BstMBI GATC 2 cut(s) 138, 264
BstMWI GCNNNNNNNGC 2 cut(s) 620, 703
BstPI GGTNACC 1 cut(s) 119
BstV1I GCAGC 2 cut(s) 218, 407
BstV2I GAAGAC 2 cut(s) 24, 138
BsuRI GGCC 1 cut(s) 399
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 3 cut(s) 457, 574, 586
BtsIMutI CAGTG 1 cut(s) 614
Cac8I GCNNGC 4 cut(s) 56, 284, 425, 695
Cfr13I GGNCC 1 cut(s) 397
CseI GACGC 1 cut(s) 223
Csp6I GTAC 4 cut(s) 50, 346, 433, 491
CviAII CATG 1 cut(s) 11
CviQI GTAC 4 cut(s) 50, 346, 433, 491
DdeI CTNAG 5 cut(s) 162, 258, 428, 494, 698
DpnI GATC 2 cut(s) 140, 266
DpnII GATC 2 cut(s) 138, 264
Eam1104I CTCTTC 2 cut(s) 243, 525
EarI CTCTTC 2 cut(s) 243, 525
EciI GGCGGA 1 cut(s) 571
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 373
Eco32I GATATC 1 cut(s) 37
Eco57I CTGAAG 2 cut(s) 347, 689
Eco91I GGTNACC 1 cut(s) 119
EcoO65I GGTNACC 1 cut(s) 119
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 373
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 1 cut(s) 14
FaiI YATR 6 cut(s) 12, 70, 444, 507, 687, 689
FaqI GGGAC 1 cut(s) 360
FatI CATG 1 cut(s) 10
FbaI TGATCA 1 cut(s) 264
Fnu4HI GCNGC 3 cut(s) 232, 400, 421
FokI GGATG 3 cut(s) 464, 573, 581
FriOI GRGCYC 1 cut(s) 373
Fsp4HI GCNGC 3 cut(s) 232, 400, 421
FspBI CTAG 1 cut(s) 596
GluI GCNGC 3 cut(s) 232, 400, 421
GsuI CTGGAG 1 cut(s) 638
HaeIII GGCC 1 cut(s) 399
HapII CCGG 2 cut(s) 228, 296
HgaI GACGC 1 cut(s) 223
Hin1II CATG 1 cut(s) 14
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 2 cut(s) 256, 535
HpaII CCGG 2 cut(s) 228, 296
HphI GGTGA 3 cut(s) 26, 131, 259
Hpy188I TCNGA 4 cut(s) 131, 138, 366, 529
Hpy188III TCNNGA 2 cut(s) 164, 211
HpyAV CCTTC 2 cut(s) 643, 664
HpyCH4IV ACGT 1 cut(s) 201
HpyCH4V TGCA 2 cut(s) 505, 646
HpyF10VI GCNNNNNNNGC 2 cut(s) 620, 703
HpyF3I CTNAG 5 cut(s) 162, 258, 428, 494, 698
HpySE526I ACGT 1 cut(s) 201
Hsp92II CATG 1 cut(s) 14
Ksp22I TGATCA 1 cut(s) 264
Kzo9I GATC 2 cut(s) 138, 264
LguI GCTCTTC 1 cut(s) 243
LmnI GCTCC 1 cut(s) 358
Lsp1109I GCAGC 2 cut(s) 218, 407
MaeI CTAG 1 cut(s) 596
MaeII ACGT 1 cut(s) 201
MaeIII GTNAC 2 cut(s) 119, 662
MalI GATC 2 cut(s) 140, 266
MboI GATC 2 cut(s) 138, 264
MboII GAAGA 5 cut(s) 29, 138, 260, 284, 542
MhlI GDGCHC 1 cut(s) 373
MluCI AATT 5 cut(s) 29, 106, 237, 303, 403
MlyI GAGTC 1 cut(s) 250
MseI TTAA 1 cut(s) 456
MspA1I CMGCKG 1 cut(s) 117
MspI CCGG 2 cut(s) 228, 296
MwoI GCNNNNNNNGC 2 cut(s) 620, 703
NcoI CCATGG 1 cut(s) 10
NdeII GATC 2 cut(s) 138, 264
NlaIII CATG 1 cut(s) 14
NmuCI GTSAC 2 cut(s) 119, 662
PciSI GCTCTTC 1 cut(s) 243
PcsI WCGNNNNNNNCGW 1 cut(s) 39
PfeI GAWTC 1 cut(s) 535
PkrI GCNGC 3 cut(s) 233, 401, 422
PleI GAGTC 1 cut(s) 250
PpsI GAGTC 1 cut(s) 250
Ppu21I YACGTR 1 cut(s) 202
PspEI GGTNACC 1 cut(s) 119
PspPI GGNCC 1 cut(s) 397
RsaI GTAC 4 cut(s) 51, 347, 434, 492
RsaNI GTAC 4 cut(s) 50, 346, 433, 491
SapI GCTCTTC 1 cut(s) 243
SaqAI TTAA 1 cut(s) 456
SatI GCNGC 3 cut(s) 232, 400, 421
Sau3AI GATC 2 cut(s) 138, 264
Sau96I GGNCC 1 cut(s) 397
SchI GAGTC 1 cut(s) 250
SduI GDGCHC 1 cut(s) 373
Sse9I AATT 5 cut(s) 29, 106, 237, 303, 403
SsiI CCGC 6 cut(s) 117, 185, 196, 280, 400, 556
SspMI CTAG 1 cut(s) 596
StyI CCWWGG 1 cut(s) 10
TaiI ACGT 1 cut(s) 204
TaqI TCGA 2 cut(s) 33, 91
TasI AATT 5 cut(s) 29, 106, 237, 303, 403
TatI WGTACW 1 cut(s) 490
TauI GCSGC 1 cut(s) 402
TfiI GAWTC 1 cut(s) 535
Tru1I TTAA 1 cut(s) 456
Tru9I TTAA 1 cut(s) 456
TscAI CASTG 1 cut(s) 621
TseFI GTSAC 2 cut(s) 119, 662
TseI GCWGC 2 cut(s) 231, 420
Tsp45I GTSAC 2 cut(s) 119, 662
TspDTI ATGAA 2 cut(s) 57, 585
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 1 cut(s) 621
XspI CTAG 1 cut(s) 596
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.