Rroxscaffold_3G00221600

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0006900
Physical Location & Seq
Forward (+)
4373659 .. 4377050
3392 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00221600.1

Sequence Viewer

Length: 759 bp
ATGTCAAACCATGGTGAAGACCTCTCTCAATTCGACATCTCCTTTCAGGAAAAGGACAAGCTCGTAGCGGAAGTAATCCGCTACGTGCTTTTCAAGACGCACCACAACTCCGGCTGCCCAATTAGGAGAGAAGAGCTGACTCAGTTGATCACCAAAAACTACCGCCAGCGAAATCTTCCGGTGCTAATTATCAACGAGGCAATAAACAAGCTCTCCACCATTTTCGGGTACGAAATGAGGGAGCTTCAGAGGGCTCGGCCCTCTTCCAATAACCATCAGGGCCGCAATTCTCAACAAGGTTCGGCAGAGGCGAAAACGTATGTTATCATGAGTAAGCTGCCTGCTGAGGTGTACAAGAAGTATGTTGAGGATGTTAATACGGCTCATCTTACTGGTTTTACTTTCGTTGTACTGAGTCTTGTACATATTTCGGGTGGTAAAATCTCCGAAGGTATGGCCTTGCTTGTTGAACTTACTCCTTGTTTTCCTGGAGCTGCAGAGAATCTTTGGCAACATTTGAGGCGGATAGGACTGGATGAAACCAACGAAAGCCATCCAGTTCTAGGAAACACAAAACAAGCACTGGAGGCACTTGTTCAGCAAAGGTATTTGCAGAAGGACAAACAAAGTGGACCTGAAGGCAATACTTTGTTATATGAGCTTGCTGAGAGAGCTTTAGATTCTCAAGTTACTGCAACCATGAAAGATTACATATCGCAGATTGTGAATAAAGATGTTGGCTCAGTGGAGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

252

Amino Acids

28.47

Weight (kDa)

6.09

Isoelectric Point (pI)

41.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 25 - 226 1.9e-39 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 68, 79, 163, 283, 523
AcuI CTGAAG 2 cut(s) 230, 657
AfaI GTAC 4 cut(s) 230, 353, 411, 423
AfiI CCNNNNNNNGG 2 cut(s) 225, 563
AgsI TTSAA 2 cut(s) 94, 470
AjnI CCWGG 1 cut(s) 487
AluBI AGCT 8 cut(s) 61, 136, 211, 244, 337, 494, 661, 674
AluI AGCT 8 cut(s) 61, 136, 211, 244, 337, 494, 661, 674
AoxI GGCC 3 cut(s) 257, 280, 456
ApeKI GCWGC 3 cut(s) 114, 337, 494
AspS9I GGNCC 3 cut(s) 258, 280, 632
AsuHPI GGTGA 2 cut(s) 26, 142
AvaII GGWCC 1 cut(s) 632
BanII GRGCYC 1 cut(s) 256
BbsI GAAGAC 1 cut(s) 24
BbvCI CCTCAGC 1 cut(s) 345
BbvI GCAGC 3 cut(s) 101, 324, 481
BccI CCATC 2 cut(s) 282, 561
BceAI ACGGC 1 cut(s) 396
BciT130I CCWGG 1 cut(s) 489
BclI TGATCA 1 cut(s) 147
BfaI CTAG 1 cut(s) 563
BfmI CTRYAG 1 cut(s) 495
BisI GCNGC 4 cut(s) 115, 283, 338, 495
BlsI GCNGC 4 cut(s) 116, 284, 339, 496
Bme1390I CCNGG 1 cut(s) 489
Bme18I GGWCC 1 cut(s) 632
BmgT120I GGNCC 3 cut(s) 258, 280, 632
BmrFI CCNGG 1 cut(s) 489
BpiI GAAGAC 1 cut(s) 24
BpmI CTGGAG 2 cut(s) 510, 605
Bpu10I CCTNAGC 1 cut(s) 345
BpuEI CTTGAG 1 cut(s) 669
BsaAI YACGTR 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 5 cut(s) 92, 122, 197, 227, 740
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 563
Bse1I ACTGG 4 cut(s) 397, 537, 557, 588
BseBI CCWGG 1 cut(s) 489
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 4 cut(s) 376, 541, 553, 757
BseLI CCNNNNNNNGG 2 cut(s) 225, 563
BseMII CTCAG 5 cut(s) 155, 336, 404, 657, 756
BseNI ACTGG 4 cut(s) 397, 537, 557, 588
BseXI GCAGC 3 cut(s) 101, 324, 481
BshFI GGCC 3 cut(s) 259, 282, 458
BsiSI CCGG 2 cut(s) 111, 179
BslI CCNNNNNNNGG 2 cut(s) 225, 563
BsnI GGCC 3 cut(s) 259, 282, 458
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 2 cut(s) 351, 421
Bsp143I GATC 1 cut(s) 147
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 5 cut(s) 68, 79, 163, 283, 523
BspANI GGCC 3 cut(s) 259, 282, 458
BspCNI CTCAG 5 cut(s) 154, 337, 405, 658, 755
BspHI TCATGA 1 cut(s) 327
BspMAI CTGCAG 1 cut(s) 499
BspQI GCTCTTC 1 cut(s) 126
BsrGI TGTACA 2 cut(s) 351, 421
BsrI ACTGG 4 cut(s) 397, 537, 557, 588
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 1 cut(s) 147
BssT1I CCWWGG 1 cut(s) 10
Bst2UI CCWGG 1 cut(s) 489
Bst6I CTCTTC 2 cut(s) 126, 268
BstAUI TGTACA 2 cut(s) 351, 421
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 3 cut(s) 167, 342, 663
BstDEI CTNAG 5 cut(s) 141, 345, 413, 666, 742
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 4 cut(s) 376, 541, 553, 757
BstKTI GATC 1 cut(s) 150
BstMBI GATC 1 cut(s) 147
BstMWI GCNNNNNNNGC 2 cut(s) 587, 671
BstNI CCWGG 1 cut(s) 489
BstSCI CCNGG 1 cut(s) 487
BstSFI CTRYAG 1 cut(s) 495
BstV1I GCAGC 3 cut(s) 101, 324, 481
BstV2I GAAGAC 1 cut(s) 24
BsuRI GGCC 3 cut(s) 259, 282, 458
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 4 cut(s) 376, 541, 553, 757
BtsIMutI CAGTG 2 cut(s) 581, 750
Cac8I GCNNGC 3 cut(s) 167, 342, 663
CciI TCATGA 1 cut(s) 327
Cfr13I GGNCC 3 cut(s) 258, 280, 632
CseI GACGC 1 cut(s) 106
Csp6I GTAC 4 cut(s) 229, 352, 410, 422
CspCI CAANNNNNGTGG 2 cut(s) 610, 645
CviAII CATG 3 cut(s) 11, 328, 700
CviQI GTAC 4 cut(s) 229, 352, 410, 422
DdeI CTNAG 5 cut(s) 141, 345, 413, 666, 742
DpnI GATC 1 cut(s) 149
DpnII GATC 1 cut(s) 147
Eam1104I CTCTTC 2 cut(s) 126, 268
EarI CTCTTC 2 cut(s) 126, 268
EciI GGCGGA 1 cut(s) 538
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 256
Eco47I GGWCC 1 cut(s) 632
Eco57I CTGAAG 2 cut(s) 230, 657
EcoRII CCWGG 1 cut(s) 487
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 3 cut(s) 14, 331, 703
FatI CATG 3 cut(s) 10, 327, 699
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 4 cut(s) 115, 283, 338, 495
FokI GGATG 3 cut(s) 383, 540, 548
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 4 cut(s) 115, 283, 338, 495
FspBI CTAG 1 cut(s) 563
GluI GCNGC 4 cut(s) 115, 283, 338, 495
GsuI CTGGAG 2 cut(s) 510, 605
HaeIII GGCC 3 cut(s) 259, 282, 458
HapII CCGG 2 cut(s) 111, 179
HgaI GACGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 14, 331, 703
HinfI GANTC 4 cut(s) 139, 415, 502, 680
HpaII CCGG 2 cut(s) 111, 179
HphI GGTGA 2 cut(s) 26, 142
Hpy166II GTNNAC 2 cut(s) 352, 632
Hpy188I TCNGA 2 cut(s) 249, 448
Hpy188III TCNNGA 3 cut(s) 47, 94, 328
Hpy8I GTNNAC 2 cut(s) 352, 632
HpyAV CCTTC 3 cut(s) 443, 610, 632
HpyCH4IV ACGT 2 cut(s) 84, 317
HpyCH4V TGCA 3 cut(s) 497, 613, 695
HpyF10VI GCNNNNNNNGC 2 cut(s) 587, 671
HpyF3I CTNAG 5 cut(s) 141, 345, 413, 666, 742
HpySE526I ACGT 2 cut(s) 84, 317
Hsp92II CATG 3 cut(s) 14, 331, 703
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 1 cut(s) 147
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 2 cut(s) 241, 491
Lsp1109I GCAGC 3 cut(s) 101, 324, 481
MaeI CTAG 1 cut(s) 563
MaeII ACGT 2 cut(s) 84, 317
MaeIII GTNAC 1 cut(s) 688
MalI GATC 1 cut(s) 149
MboI GATC 1 cut(s) 147
MboII GAAGA 4 cut(s) 29, 143, 167, 255
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 4 cut(s) 29, 120, 186, 286
MlyI GAGTC 2 cut(s) 133, 424
MseI TTAA 1 cut(s) 375
MspI CCGG 2 cut(s) 111, 179
MspR9I CCNGG 1 cut(s) 489
MvaI CCWGG 1 cut(s) 489
MwoI GCNNNNNNNGC 2 cut(s) 587, 671
NcoI CCATGG 1 cut(s) 10
NdeII GATC 1 cut(s) 147
NlaIII CATG 3 cut(s) 14, 331, 703
NmeAIII GCCGAG 1 cut(s) 235
PagI TCATGA 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 1 cut(s) 308
PfeI GAWTC 2 cut(s) 502, 680
PfoI TCCNGGA 1 cut(s) 487
PkrI GCNGC 4 cut(s) 116, 284, 339, 496
PleI GAGTC 2 cut(s) 133, 423
PpsI GAGTC 2 cut(s) 133, 423
Ppu21I YACGTR 1 cut(s) 85
Psp6I CCWGG 1 cut(s) 487
PspGI CCWGG 1 cut(s) 487
PspPI GGNCC 3 cut(s) 258, 280, 632
PstI CTGCAG 1 cut(s) 499
RsaI GTAC 4 cut(s) 230, 353, 411, 423
RsaNI GTAC 4 cut(s) 229, 352, 410, 422
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 4 cut(s) 115, 283, 338, 495
Sau3AI GATC 1 cut(s) 147
Sau96I GGNCC 3 cut(s) 258, 280, 632
SchI GAGTC 2 cut(s) 133, 424
ScrFI CCNGG 1 cut(s) 489
SduI GDGCHC 1 cut(s) 256
SfcI CTRYAG 1 cut(s) 495
SinI GGWCC 1 cut(s) 632
SmlI CTYRAG 1 cut(s) 684
SmoI CTYRAG 1 cut(s) 684
Sse9I AATT 4 cut(s) 29, 120, 186, 286
SsiI CCGC 5 cut(s) 68, 79, 163, 283, 523
SspMI CTAG 1 cut(s) 563
StyD4I CCNGG 1 cut(s) 487
StyI CCWWGG 1 cut(s) 10
TaiI ACGT 2 cut(s) 87, 320
TaqI TCGA 1 cut(s) 33
TasI AATT 4 cut(s) 29, 120, 186, 286
TatI WGTACW 3 cut(s) 351, 409, 421
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 2 cut(s) 502, 680
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 588, 750
TseI GCWGC 3 cut(s) 114, 337, 494
TspDTI ATGAA 2 cut(s) 552, 716
TspRI CASTG 2 cut(s) 588, 750
VpaK11BI GGWCC 1 cut(s) 632
XspI CTAG 1 cut(s) 563
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.