RLG00000000817

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0006900
Physical Location & Seq
Forward (+)
5923532 .. 5925947
2416 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000000817

Sequence Viewer

Length: 711 bp
ATGTCAAACCATGGTGAAGATCTCTCTCAATTCGATATCTCCGTTCAGGAAAAGGACAAGCTCGTAGCGGAAGTAATCCGCTACGTGCTTTTCAAGACGCACCACAACTCCGGCTGCCCAATTAGGAGAGAAGAGCTGACTCAGTTGATCACCAAAAACTACCGCCAGCGAAATCTTCCGGTGCTAATTATCAACGAGGCAATAAACAAGCTCTCCACCATTTTCGGGTACGAAATGAGGGAGCTTCAGAGGGCTCGCCCCTCTTCCAATAACCATCAGGGCCGCAATTCTCAACAAGGTTCGGCAGAGGCGAAAACGTATGTTATCATGAGTAAGTTGCCTGCTGAAGTGTACAAGAAGTATGTTGAGGATGTTAATACGGCTCATCTTACTGGATTTACTTTCGTTGTACTGAGTATTGTACATATTTCGGGTGGTAAAATCTCCGAAGAGAATCTGTGGCAACATTTGAGGCGGATAGGACTGGATGAAACTAATGAAAGCCATCCAGTTCTAGGAAACACAAAACAAGCACTGGAGGCACTTGTCCAGCAAAGGTATTTGCAGAAGGACAAACAAAGTGGACCTGAAGGCAATACTTTGTTATATGAGCTTGCTGAGAGAGCTTTAGATTCTCAAGTTATTGCAACCATGAAAGATTACATATCGCAGATTGTGAATAAAGATGTTGGCTCAGTGGAGGATGAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

237

Amino Acids

26.87

Weight (kDa)

6.26

Isoelectric Point (pI)

44.52

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MAGE PF01454 25 - 210 1.2e-45 MAGE homology domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 68, 79, 163, 283, 475
AcuI CTGAAG 3 cut(s) 230, 366, 609
AfaI GTAC 4 cut(s) 230, 353, 411, 423
AfiI CCNNNNNNNGG 2 cut(s) 225, 515
AgsI TTSAA 1 cut(s) 94
AluBI AGCT 6 cut(s) 61, 136, 211, 244, 613, 626
AluI AGCT 6 cut(s) 61, 136, 211, 244, 613, 626
AoxI GGCC 1 cut(s) 280
ApeKI GCWGC 1 cut(s) 114
AspS9I GGNCC 2 cut(s) 280, 584
AsuHPI GGTGA 2 cut(s) 26, 142
AvaII GGWCC 1 cut(s) 584
BanII GRGCYC 1 cut(s) 256
BbvI GCAGC 1 cut(s) 101
BccI CCATC 2 cut(s) 282, 513
BceAI ACGGC 1 cut(s) 396
BclI TGATCA 1 cut(s) 147
BfaI CTAG 1 cut(s) 515
BglII AGATCT 1 cut(s) 19
BisI GCNGC 2 cut(s) 115, 283
BlsI GCNGC 2 cut(s) 116, 284
Bme18I GGWCC 1 cut(s) 584
BmgT120I GGNCC 2 cut(s) 280, 584
BpmI CTGGAG 1 cut(s) 557
BpuEI CTTGAG 1 cut(s) 621
BsaAI YACGTR 1 cut(s) 85
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 1 cut(s) 178
BsaXI ACNNNNNCTCC 5 cut(s) 92, 122, 197, 227, 692
Bsc4I CCNNNNNNNGG 2 cut(s) 225, 515
Bse1I ACTGG 4 cut(s) 397, 489, 509, 540
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 4 cut(s) 376, 493, 505, 709
BseLI CCNNNNNNNGG 2 cut(s) 225, 515
BseMII CTCAG 4 cut(s) 155, 404, 609, 708
BseNI ACTGG 4 cut(s) 397, 489, 509, 540
BseXI GCAGC 1 cut(s) 101
BshFI GGCC 1 cut(s) 282
BsiSI CCGG 2 cut(s) 111, 179
BslI CCNNNNNNNGG 2 cut(s) 225, 515
BsnI GGCC 1 cut(s) 282
Bsp1286I GDGCHC 1 cut(s) 256
Bsp1407I TGTACA 2 cut(s) 351, 421
Bsp143I GATC 2 cut(s) 19, 147
Bsp19I CCATGG 1 cut(s) 10
BspACI CCGC 5 cut(s) 68, 79, 163, 283, 475
BspANI GGCC 1 cut(s) 282
BspCNI CTCAG 4 cut(s) 154, 405, 610, 707
BspHI TCATGA 1 cut(s) 327
BspQI GCTCTTC 1 cut(s) 126
BsrGI TGTACA 2 cut(s) 351, 421
BsrI ACTGG 4 cut(s) 397, 489, 509, 540
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 2 cut(s) 19, 147
BssT1I CCWWGG 1 cut(s) 10
Bst6I CTCTTC 3 cut(s) 126, 268, 444
BstAUI TGTACA 2 cut(s) 351, 421
BstBAI YACGTR 1 cut(s) 85
BstC8I GCNNGC 4 cut(s) 167, 256, 342, 615
BstDEI CTNAG 4 cut(s) 141, 413, 618, 694
BstDSI CCRYGG 1 cut(s) 10
BstF5I GGATG 4 cut(s) 376, 493, 505, 709
BstKTI GATC 2 cut(s) 22, 150
BstMBI GATC 2 cut(s) 19, 147
BstMWI GCNNNNNNNGC 2 cut(s) 539, 623
BstV1I GCAGC 1 cut(s) 101
BstX2I RGATCY 1 cut(s) 19
BstYI RGATCY 1 cut(s) 19
BsuRI GGCC 1 cut(s) 282
BtgI CCRYGG 1 cut(s) 10
BtsCI GGATG 4 cut(s) 376, 493, 505, 709
BtsIMutI CAGTG 2 cut(s) 533, 702
Cac8I GCNNGC 4 cut(s) 167, 256, 342, 615
CciI TCATGA 1 cut(s) 327
Cfr13I GGNCC 2 cut(s) 280, 584
CseI GACGC 1 cut(s) 106
Csp6I GTAC 4 cut(s) 229, 352, 410, 422
CspCI CAANNNNNGTGG 2 cut(s) 562, 597
CviAII CATG 3 cut(s) 11, 328, 652
CviQI GTAC 4 cut(s) 229, 352, 410, 422
DdeI CTNAG 4 cut(s) 141, 413, 618, 694
DpnI GATC 2 cut(s) 21, 149
DpnII GATC 2 cut(s) 19, 147
Eam1104I CTCTTC 3 cut(s) 126, 268, 444
EarI CTCTTC 3 cut(s) 126, 268, 444
EciI GGCGGA 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 10
Eco24I GRGCYC 1 cut(s) 256
Eco32I GATATC 1 cut(s) 37
Eco47I GGWCC 1 cut(s) 584
Eco57I CTGAAG 3 cut(s) 230, 366, 609
EcoRV GATATC 1 cut(s) 37
EcoT14I CCWWGG 1 cut(s) 10
EcoT38I GRGCYC 1 cut(s) 256
ErhI CCWWGG 1 cut(s) 10
FaeI CATG 3 cut(s) 14, 331, 655
FaiI YATR 9 cut(s) 12, 321, 329, 363, 426, 607, 609, 653, 665
FatI CATG 3 cut(s) 10, 327, 651
FbaI TGATCA 1 cut(s) 147
Fnu4HI GCNGC 2 cut(s) 115, 283
FokI GGATG 3 cut(s) 383, 492, 500
FriOI GRGCYC 1 cut(s) 256
Fsp4HI GCNGC 2 cut(s) 115, 283
FspBI CTAG 1 cut(s) 515
GluI GCNGC 2 cut(s) 115, 283
GsuI CTGGAG 1 cut(s) 557
HaeIII GGCC 1 cut(s) 282
HapII CCGG 2 cut(s) 111, 179
HgaI GACGC 1 cut(s) 106
Hin1II CATG 3 cut(s) 14, 331, 655
HinfI GANTC 3 cut(s) 139, 454, 632
HpaII CCGG 2 cut(s) 111, 179
HphI GGTGA 2 cut(s) 26, 142
Hpy166II GTNNAC 2 cut(s) 352, 584
Hpy188I TCNGA 2 cut(s) 249, 448
Hpy188III TCNNGA 3 cut(s) 47, 94, 328
Hpy8I GTNNAC 2 cut(s) 352, 584
HpyAV CCTTC 2 cut(s) 562, 584
HpyCH4IV ACGT 2 cut(s) 84, 317
HpyCH4V TGCA 2 cut(s) 565, 647
HpyF10VI GCNNNNNNNGC 2 cut(s) 539, 623
HpyF3I CTNAG 4 cut(s) 141, 413, 618, 694
HpySE526I ACGT 2 cut(s) 84, 317
Hsp92II CATG 3 cut(s) 14, 331, 655
Ksp22I TGATCA 1 cut(s) 147
Kzo9I GATC 2 cut(s) 19, 147
LguI GCTCTTC 1 cut(s) 126
LmnI GCTCC 1 cut(s) 241
Lsp1109I GCAGC 1 cut(s) 101
MaeI CTAG 1 cut(s) 515
MaeII ACGT 2 cut(s) 84, 317
MalI GATC 2 cut(s) 21, 149
MboI GATC 2 cut(s) 19, 147
MboII GAAGA 5 cut(s) 29, 143, 167, 255, 461
MflI RGATCY 1 cut(s) 19
MhlI GDGCHC 1 cut(s) 256
MluCI AATT 4 cut(s) 29, 120, 186, 286
MlyI GAGTC 1 cut(s) 133
MnlI CCTC 9 cut(s) 190, 231, 243, 271, 301, 361, 465, 532, 694
MseI TTAA 1 cut(s) 375
MspI CCGG 2 cut(s) 111, 179
MwoI GCNNNNNNNGC 2 cut(s) 539, 623
NcoI CCATGG 1 cut(s) 10
NdeII GATC 2 cut(s) 19, 147
NlaIII CATG 3 cut(s) 14, 331, 655
PagI TCATGA 1 cut(s) 327
PciSI GCTCTTC 1 cut(s) 126
PcsI WCGNNNNNNNCGW 2 cut(s) 39, 308
PfeI GAWTC 2 cut(s) 454, 632
PkrI GCNGC 2 cut(s) 116, 284
PleI GAGTC 1 cut(s) 133
PpsI GAGTC 1 cut(s) 133
Ppu21I YACGTR 1 cut(s) 85
PspPI GGNCC 2 cut(s) 280, 584
PsuI RGATCY 1 cut(s) 19
RsaI GTAC 4 cut(s) 230, 353, 411, 423
RsaNI GTAC 4 cut(s) 229, 352, 410, 422
SapI GCTCTTC 1 cut(s) 126
SaqAI TTAA 1 cut(s) 375
SatI GCNGC 2 cut(s) 115, 283
Sau3AI GATC 2 cut(s) 19, 147
Sau96I GGNCC 2 cut(s) 280, 584
SchI GAGTC 1 cut(s) 133
SduI GDGCHC 1 cut(s) 256
SinI GGWCC 1 cut(s) 584
SmlI CTYRAG 1 cut(s) 636
SmoI CTYRAG 1 cut(s) 636
Sse9I AATT 4 cut(s) 29, 120, 186, 286
SsiI CCGC 5 cut(s) 68, 79, 163, 283, 475
SspMI CTAG 1 cut(s) 515
StyI CCWWGG 1 cut(s) 10
TaiI ACGT 2 cut(s) 87, 320
TaqI TCGA 1 cut(s) 33
TasI AATT 4 cut(s) 29, 120, 186, 286
TatI WGTACW 3 cut(s) 351, 409, 421
TauI GCSGC 1 cut(s) 285
TfiI GAWTC 2 cut(s) 454, 632
Tru1I TTAA 1 cut(s) 375
Tru9I TTAA 1 cut(s) 375
TscAI CASTG 2 cut(s) 540, 702
TseI GCWGC 1 cut(s) 114
TspDTI ATGAA 3 cut(s) 504, 513, 668
TspGWI ACGGA 1 cut(s) 31
TspRI CASTG 2 cut(s) 540, 702
VpaK11BI GGWCC 1 cut(s) 584
XspI CTAG 1 cut(s) 515
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.