Rorug07G0323100

Melanoma-associated antigen

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000007
OG0006900
Physical Location & Seq
Reverse (-)
33312265 .. 33315737
3473 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug07G0323100.1

Sequence Viewer

Length: 1266 bp
ATGGCTCTGGTCCTGCATGCAGGGAGTACAAACAAAAATGCATACAAGACTCTCATTGCTGCAGAGTATACAGGTGTGAAAGTTGCACTTGCACCCAACTTTGAGATGGGCGTCTCGAACAAGACTCCTGAATTTCTCAAGATGAACCCGATTGGGAAGGTTCCTGTGCTGGAAACACCTGATGGTCCCATCTTCGAGAGTAATGCCATTGCACGTTACGTTACTCACTTGAAGGCTGACAATCCTCTTTATGGTTCATCTTCAATTGATTATGCCCATATCGAGCAATGGATTGATTTTGGATCCTTGGAGATTGATGCTAACATTGTTAATTGGTTTAGACCAAGAATGGGAAGGGCTGTCTATCTTCCTCCAGCAGAGGAAGCTGCAATTGCTGCATTGAAGAGAGCACTGGGTGCATTGAACACACACCTTGCTTCCTGCACATACCTTGTTGGGGATTCTGTCACCCTTGCTGACATCATTATGACATGCAATTTGTTTATGGGCTTCACTAAGCTCATGACTAAGAGCTTCACTTCAGAGTTTCCTCATGTTGAGAGATACTTTTGGACCATGGTCAATCAACCAAACTTCAAAAAGGTATTGGGTGAGGTTAAGCAGACGGATTCTGTTCCTGCTGTTCAATCTGCAAAGAAGCCTGCCCAGGCCAAAGAATCTGCTAAACCCAAGGCTAAGGAGGAGCCAAAGAAAGAAGCCAAGAAGGAGCCGGCAAAGCCCAAAGCAGAAGTTGAGGAGGAAGAGGCACCAAAGCCAAAACCCAAGAATCCTCTTGATCTGCTTCCCCCGAGTAAGATGATATTGGATGACTGGAAAAGGCTTTACTCTAACACGAAGACCAACTTCCGTGAGGTGGCAATTAAAGGATTCTGGGACATGTACGATCCTGAGGGATATTCTCTTTGGTTCTGTGATTACAAGTACAATGATGAGAATACTGTTTCCTTCGTGACGCTGAACAAGGTCGGTGGATTTCTCCAACGGATGGATCTGGCCCGCAAGTATGCTTTTGGGAAGATGCTAATTATTGGTGCAAACCCACCATACAAGGTGAAGGGCTTGTGGCTCTTCCGTGGCCCAGAAATCCCTGAATTTGTGATGAATGAGTGCTATGACATGGAGCTTTATGAGTGGACCAAGGTCGACATCTCAGATGAAAACCAAAAGGAGCGTGTGAGTCAGATGATTGAAGACCAGGAGCCTTTTGAGGGAGAGCCTCTTTTGGATGCCAAATGTTTCAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MAGE

Protein Analysis

421

Amino Acids

47.7

Weight (kDa)

6.1

Isoelectric Point (pI)

37.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
GST_N PF02798 8 - 74 6.2e-14 Glutathione S-transferase, N-terminal domain
GST_N_2 PF13409 14 - 76 3.6e-08 Glutathione S-transferase, N-terminal domain
GST_N_3 PF13417 16 - 81 6.2e-07 Glutathione S-transferase, N-terminal domain
GST_C PF00043 126 - 197 4.5e-12 Glutathione S-transferase, C-terminal domain
EF1G PF00647 260 - 367 2.5e-41 Elongation factor 1 gamma, conserved domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 766
AccB7I CCANNNNNTGG 1 cut(s) 1006
AccI GTMKAC 2 cut(s) 68, 1164
AciI CCGC 1 cut(s) 1018
AclWI GGATC 4 cut(s) 297, 310, 899, 1017
AcsI RAATTY 2 cut(s) 131, 1112
AcuI CTGAAG 1 cut(s) 525
AcyI GRCGYC 1 cut(s) 111
AdeI CACNNNGTG 1 cut(s) 416
AfaI GTAC 3 cut(s) 28, 902, 944
AfiI CCNNNNNNNGG 6 cut(s) 251, 350, 457, 874, 1006, 1229
AflIII ACRYGT 1 cut(s) 897
AgsI TTSAA 8 cut(s) 232, 264, 403, 424, 598, 647, 1211, 1261
AjnI CCWGG 2 cut(s) 666, 1215
AluBI AGCT 4 cut(s) 386, 520, 534, 1144
AluI AGCT 4 cut(s) 386, 520, 534, 1144
Alw21I GWGCWC 1 cut(s) 412
Alw26I GTCTC 1 cut(s) 118
AlwI GGATC 4 cut(s) 297, 310, 899, 1017
Ama87I CYCGRG 1 cut(s) 808
AoxI GGCC 3 cut(s) 669, 1014, 1096
ApeKI GCWGC 3 cut(s) 59, 386, 395
ApoI RAATTY 2 cut(s) 131, 1112
ArsI GACNNNNNNTTYG 2 cut(s) 481, 513
AspS9I GGNCC 6 cut(s) 10, 185, 573, 1015, 1097, 1155
AsuHPI GGTGA 3 cut(s) 460, 623, 1084
AvaI CYCGRG 1 cut(s) 808
AvaII GGWCC 4 cut(s) 10, 185, 573, 1155
AxyI CCTNAGG 1 cut(s) 909
BamHI GGATCC 1 cut(s) 302
BanI GGYRCC 1 cut(s) 766
BbsI GAAGAC 2 cut(s) 863, 1218
Bbv12I GWGCWC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 46, 373, 382
BccI CCATC 4 cut(s) 100, 176, 197, 1000
BcgI CGANNNNNNTGC 2 cut(s) 185, 219
BciT130I CCWGG 2 cut(s) 668, 1217
BcoDI GTCTC 1 cut(s) 118
BfmI CTRYAG 1 cut(s) 60
BisI GCNGC 3 cut(s) 60, 387, 396
BlsI GCNGC 3 cut(s) 61, 388, 397
Bme1390I CCNGG 2 cut(s) 668, 1217
Bme18I GGWCC 4 cut(s) 10, 185, 573, 1155
BmeT110I CYCGRG 1 cut(s) 808
BmgT120I GGNCC 6 cut(s) 10, 185, 573, 1015, 1097, 1155
BmiI GGNNCC 7 cut(s) 162, 187, 304, 705, 729, 768, 1221
BmrFI CCNGG 2 cut(s) 668, 1217
BmrI ACTGGG 1 cut(s) 422
BmsI GCATC 3 cut(s) 307, 1029, 1237
BmuI ACTGGG 1 cut(s) 422
BoxI GACNNNNGTC 2 cut(s) 578, 1160
BpiI GAAGAC 2 cut(s) 863, 1218
BpmI CTGGAG 1 cut(s) 357
Bpu10I CCTNAGC 1 cut(s) 696
BpuEI CTTGAG 1 cut(s) 122
BsaHI GRCGYC 1 cut(s) 111
BsaJI CCNNGG 6 cut(s) 306, 576, 666, 690, 1093, 1158
Bsc4I CCNNNNNNNGG 6 cut(s) 251, 350, 457, 874, 1006, 1229
Bse118I RCCGGY 1 cut(s) 730
Bse1I ACTGG 2 cut(s) 417, 836
Bse21I CCTNAGG 1 cut(s) 909
Bse3DI GCAATG 3 cut(s) 54, 207, 293
BseBI CCWGG 2 cut(s) 668, 1217
BseDI CCNNGG 6 cut(s) 306, 576, 666, 690, 1093, 1158
BseGI GGATG 3 cut(s) 832, 1011, 1252
BseLI CCNNNNNNNGG 6 cut(s) 251, 350, 457, 874, 1006, 1229
BseMI GCAATG 3 cut(s) 54, 207, 293
BseMII CTCAG 2 cut(s) 900, 1185
BseNI ACTGG 2 cut(s) 417, 836
BseRI GAGGAG 2 cut(s) 716, 770
BseXI GCAGC 3 cut(s) 46, 373, 382
BsgI GTGCAG 1 cut(s) 427
BshFI GGCC 3 cut(s) 671, 1016, 1098
BshNI GGYRCC 1 cut(s) 766
BsiHKAI GWGCWC 1 cut(s) 412
BsiHKCI CYCGRG 1 cut(s) 808
BsiSI CCGG 1 cut(s) 731
BslFI GGGAC 2 cut(s) 171, 908
BslI CCNNNNNNNGG 6 cut(s) 251, 350, 457, 874, 1006, 1229
BsmAI GTCTC 1 cut(s) 118
BsmBI CGTCTC 1 cut(s) 118
BsmFI GGGAC 2 cut(s) 171, 908
BsnI GGCC 3 cut(s) 671, 1016, 1098
BsoBI CYCGRG 1 cut(s) 808
Bsp1286I GDGCHC 1 cut(s) 412
Bsp143I GATC 4 cut(s) 302, 796, 904, 1009
Bsp19I CCATGG 1 cut(s) 576
BspACI CCGC 1 cut(s) 1018
BspANI GGCC 3 cut(s) 671, 1016, 1098
BspCNI CTCAG 2 cut(s) 901, 1184
BspHI TCATGA 1 cut(s) 522
BspLI GGNNCC 7 cut(s) 162, 187, 304, 705, 729, 768, 1221
BspMAI CTGCAG 1 cut(s) 64
BspPI GGATC 4 cut(s) 297, 310, 899, 1017
BspQI GCTCTTC 1 cut(s) 1094
BspT107I GGYRCC 1 cut(s) 766
BsrDI GCAATG 3 cut(s) 54, 207, 293
BsrFI RCCGGY 1 cut(s) 730
BsrI ACTGG 2 cut(s) 417, 836
BssAI RCCGGY 1 cut(s) 730
BssECI CCNNGG 6 cut(s) 306, 576, 666, 690, 1093, 1158
BssMI GATC 4 cut(s) 302, 796, 904, 1009
BssNAI GTATAC 1 cut(s) 69
BssNI GRCGYC 1 cut(s) 111
BssT1I CCWWGG 4 cut(s) 306, 576, 690, 1158
Bst1107I GTATAC 1 cut(s) 69
Bst2UI CCWGG 2 cut(s) 668, 1217
Bst4CI ACNGT 1 cut(s) 961
Bst6I CTCTTC 3 cut(s) 398, 756, 1094
BstACI GRCGYC 1 cut(s) 111
BstAPI GCANNNNNTGC 2 cut(s) 395, 416
BstC8I GCNNGC 4 cut(s) 18, 663, 732, 1018
BstDEI CTNAG 5 cut(s) 516, 528, 696, 909, 1171
BstDSI CCRYGG 2 cut(s) 576, 1093
BstF5I GGATG 3 cut(s) 832, 1011, 1252
BstKTI GATC 4 cut(s) 305, 799, 907, 1012
BstMAI GTCTC 1 cut(s) 118
BstMBI GATC 4 cut(s) 302, 796, 904, 1009
BstMWI GCNNNNNNNGC 5 cut(s) 383, 392, 395, 416, 736
BstNI CCWGG 2 cut(s) 668, 1217
BstNSI RCATGY 3 cut(s) 20, 495, 901
BstPAI GACNNNNGTC 2 cut(s) 578, 1160
BstSCI CCNGG 2 cut(s) 666, 1215
BstSFI CTRYAG 1 cut(s) 60
BstV1I GCAGC 3 cut(s) 46, 373, 382
BstV2I GAAGAC 2 cut(s) 863, 1218
BstX2I RGATCY 2 cut(s) 302, 1009
BstYI RGATCY 2 cut(s) 302, 1009
BstZ17I GTATAC 1 cut(s) 69
Bsu36I CCTNAGG 1 cut(s) 909
BsuRI GGCC 3 cut(s) 671, 1016, 1098
BtgI CCRYGG 2 cut(s) 576, 1093
BtsCI GGATG 3 cut(s) 832, 1011, 1252
BtsIMutI CAGTG 1 cut(s) 410
Cac8I GCNNGC 4 cut(s) 18, 663, 732, 1018
CciI TCATGA 1 cut(s) 522
Cfr10I RCCGGY 1 cut(s) 730
Cfr13I GGNCC 6 cut(s) 10, 185, 573, 1015, 1097, 1155
CseI GACGC 2 cut(s) 100, 982
Csp6I GTAC 3 cut(s) 27, 901, 943
CspCI CAANNNNNGTGG 2 cut(s) 970, 1005
CviAII CATG 7 cut(s) 17, 492, 523, 554, 577, 898, 1138
CviQI GTAC 3 cut(s) 27, 901, 943
DdeI CTNAG 5 cut(s) 516, 528, 696, 909, 1171
DpnI GATC 4 cut(s) 304, 798, 906, 1011
DpnII GATC 4 cut(s) 302, 796, 904, 1009
DraIII CACNNNGTG 1 cut(s) 416
Eam1104I CTCTTC 3 cut(s) 398, 756, 1094
EarI CTCTTC 3 cut(s) 398, 756, 1094
Eco130I CCWWGG 4 cut(s) 306, 576, 690, 1158
Eco47I GGWCC 4 cut(s) 10, 185, 573, 1155
Eco57I CTGAAG 1 cut(s) 525
Eco81I CCTNAGG 1 cut(s) 909
Eco88I CYCGRG 1 cut(s) 808
EcoRII CCWGG 2 cut(s) 666, 1215
EcoT14I CCWWGG 4 cut(s) 306, 576, 690, 1158
EcoT22I ATGCAT 1 cut(s) 43
ErhI CCWWGG 4 cut(s) 306, 576, 690, 1158
Esp3I CGTCTC 1 cut(s) 118
FaeI CATG 7 cut(s) 20, 495, 526, 557, 580, 901, 1141
FalI AAGNNNNNCTT 4 cut(s) 72, 104, 848, 880
FaqI GGGAC 2 cut(s) 171, 908
FatI CATG 7 cut(s) 16, 491, 522, 553, 576, 897, 1137
FauI CCCGC 1 cut(s) 1025
FblI GTMKAC 2 cut(s) 68, 1164
Fnu4HI GCNGC 3 cut(s) 60, 387, 396
FokI GGATG 3 cut(s) 839, 1018, 1259
Fsp4HI GCNGC 3 cut(s) 60, 387, 396
GluI GCNGC 3 cut(s) 60, 387, 396
GsuI CTGGAG 1 cut(s) 357
HaeIII GGCC 3 cut(s) 671, 1016, 1098
HapII CCGG 1 cut(s) 731
HgaI GACGC 2 cut(s) 100, 982
Hin1I GRCGYC 1 cut(s) 111
Hin1II CATG 7 cut(s) 20, 495, 526, 557, 580, 901, 1141
HincII GTYRAC 1 cut(s) 1165
HindII GTYRAC 1 cut(s) 1165
HinfI GANTC 8 cut(s) 49, 124, 461, 629, 677, 787, 888, 1198
HpaII CCGG 1 cut(s) 731
HphI GGTGA 3 cut(s) 460, 623, 1084
Hpy166II GTNNAC 3 cut(s) 69, 1155, 1165
Hpy188I TCNGA 3 cut(s) 544, 1174, 1203
Hpy188III TCNNGA 8 cut(s) 115, 128, 139, 196, 523, 794, 908, 970
Hpy8I GTNNAC 3 cut(s) 69, 1155, 1165
HpyAV CCTTC 6 cut(s) 151, 226, 348, 718, 976, 1069
HpyCH4III ACNGT 1 cut(s) 961
HpyCH4IV ACGT 2 cut(s) 214, 219
HpyF10VI GCNNNNNNNGC 5 cut(s) 383, 392, 395, 416, 736
HpyF3I CTNAG 5 cut(s) 516, 528, 696, 909, 1171
HpySE526I ACGT 2 cut(s) 214, 219
Hsp92I GRCGYC 1 cut(s) 111
Hsp92II CATG 7 cut(s) 20, 495, 526, 557, 580, 901, 1141
KroI GCCGGC 1 cut(s) 730
KroNI GCCGGC 1 cut(s) 732
Kzo9I GATC 4 cut(s) 302, 796, 904, 1009
LguI GCTCTTC 1 cut(s) 1094
LmnI GCTCC 5 cut(s) 703, 727, 1141, 1189, 1219
Lsp1109I GCAGC 3 cut(s) 46, 373, 382
LweI GCATC 3 cut(s) 307, 1029, 1237
MaeII ACGT 2 cut(s) 214, 219
MaeIII GTNAC 4 cut(s) 215, 220, 466, 970
MalI GATC 4 cut(s) 304, 798, 906, 1011
MboI GATC 4 cut(s) 302, 796, 904, 1009
MboII GAAGA 9 cut(s) 184, 252, 359, 415, 773, 868, 1048, 1081, 1223
MfeI CAATTG 2 cut(s) 264, 390
MflI RGATCY 2 cut(s) 302, 1009
MhlI GDGCHC 1 cut(s) 412
MluCI AATT 8 cut(s) 131, 264, 331, 390, 496, 879, 1044, 1112
MlyI GAGTC 3 cut(s) 43, 118, 1207
MmeI TCCRAC 1 cut(s) 1024
Mph1103I ATGCAT 1 cut(s) 43
MroNI GCCGGC 1 cut(s) 730
MseI TTAA 3 cut(s) 330, 618, 882
MslI CAYNNNNRTG 1 cut(s) 485
MspI CCGG 1 cut(s) 731
MspR9I CCNGG 2 cut(s) 668, 1217
MunI CAATTG 2 cut(s) 264, 390
MvaI CCWGG 2 cut(s) 668, 1217
MwoI GCNNNNNNNGC 5 cut(s) 383, 392, 395, 416, 736
NaeI GCCGGC 1 cut(s) 732
NcoI CCATGG 1 cut(s) 576
NdeII GATC 4 cut(s) 302, 796, 904, 1009
NgoMIV GCCGGC 1 cut(s) 730
NlaIII CATG 7 cut(s) 20, 495, 526, 557, 580, 901, 1141
NlaIV GGNNCC 7 cut(s) 162, 187, 304, 705, 729, 768, 1221
NmuCI GTSAC 2 cut(s) 466, 970
NsiI ATGCAT 1 cut(s) 43
NspI RCATGY 3 cut(s) 20, 495, 901
PaeI GCATGC 1 cut(s) 20
PagI TCATGA 1 cut(s) 522
PciI ACATGT 1 cut(s) 897
PciSI GCTCTTC 1 cut(s) 1094
PdiI GCCGGC 1 cut(s) 732
PfeI GAWTC 5 cut(s) 461, 629, 677, 787, 888
PflMI CCANNNNNTGG 1 cut(s) 1006
PkrI GCNGC 3 cut(s) 61, 388, 397
PleI GAGTC 3 cut(s) 43, 118, 1206
PpsI GAGTC 3 cut(s) 43, 118, 1206
PscI ACATGT 1 cut(s) 897
PshAI GACNNNNGTC 2 cut(s) 578, 1160
Psp6I CCWGG 2 cut(s) 666, 1215
PspGI CCWGG 2 cut(s) 666, 1215
PspN4I GGNNCC 7 cut(s) 162, 187, 304, 705, 729, 768, 1221
PspPI GGNCC 6 cut(s) 10, 185, 573, 1015, 1097, 1155
PstI CTGCAG 1 cut(s) 64
PsuI RGATCY 2 cut(s) 302, 1009
RsaI GTAC 3 cut(s) 28, 902, 944
RsaNI GTAC 3 cut(s) 27, 901, 943
RseI CAYNNNNRTG 1 cut(s) 485
SalI GTCGAC 1 cut(s) 1163
SapI GCTCTTC 1 cut(s) 1094
SaqAI TTAA 3 cut(s) 330, 618, 882
SatI GCNGC 3 cut(s) 60, 387, 396
Sau3AI GATC 4 cut(s) 302, 796, 904, 1009
Sau96I GGNCC 6 cut(s) 10, 185, 573, 1015, 1097, 1155
SchI GAGTC 3 cut(s) 43, 118, 1207
ScrFI CCNGG 2 cut(s) 668, 1217
SduI GDGCHC 1 cut(s) 412
SfaNI GCATC 3 cut(s) 307, 1029, 1237
SfcI CTRYAG 1 cut(s) 60
SinI GGWCC 4 cut(s) 10, 185, 573, 1155
SmiMI CAYNNNNRTG 1 cut(s) 485
SmlI CTYRAG 1 cut(s) 137
SmoI CTYRAG 1 cut(s) 137
SphI GCATGC 1 cut(s) 20
Sse9I AATT 8 cut(s) 131, 264, 331, 390, 496, 879, 1044, 1112
SsiI CCGC 1 cut(s) 1018
StyD4I CCNGG 2 cut(s) 666, 1215
StyI CCWWGG 4 cut(s) 306, 576, 690, 1158
TaaI ACNGT 1 cut(s) 961
TaiI ACGT 2 cut(s) 217, 222
TaqI TCGA 4 cut(s) 116, 195, 282, 1164
TasI AATT 8 cut(s) 131, 264, 331, 390, 496, 879, 1044, 1112
TatI WGTACW 2 cut(s) 26, 942
TfiI GAWTC 5 cut(s) 461, 629, 677, 787, 888
Tru1I TTAA 3 cut(s) 330, 618, 882
Tru9I TTAA 3 cut(s) 330, 618, 882
TscAI CASTG 1 cut(s) 417
TseFI GTSAC 2 cut(s) 466, 970
TseI GCWGC 3 cut(s) 59, 386, 395
Tsp45I GTSAC 2 cut(s) 466, 970
TspDTI ATGAA 4 cut(s) 158, 246, 1136, 1191
TspGWI ACGGA 4 cut(s) 641, 857, 1018, 1082
TspRI CASTG 1 cut(s) 417
Van91I CCANNNNNTGG 1 cut(s) 1006
VpaK11BI GGWCC 4 cut(s) 10, 185, 573, 1155
XapI RAATTY 2 cut(s) 131, 1112
XceI RCATGY 3 cut(s) 20, 495, 901
XcmI CCANNNNNNNNNTGG 1 cut(s) 103
XmiI GTMKAC 2 cut(s) 68, 1164
Zsp2I ATGCAT 1 cut(s) 43
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.