FvH4_6g07240

Zinc finger matrin-type protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0006545
Physical Location & Seq
Forward (+)
4361978 .. 4365298
3321 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07240.t1

Sequence Viewer

Length: 609 bp
ATGGCCGCCAATGGTGCTGCAGCTGGAGTAGATAATACTCACAGAAGGAAATTCGACCTTGAAGAGTACGCTGAGAAAGCCCGCGAGCGTGAAAGAAAGGAAGAGGAGGGAGCTCGTAAGTCGAAATCAAAAGGTCCTCCGGTGCAGAGGAAGCCGTTGAAGCACAGGGATTATGAAGTAGACCTTGAATCTCGCTTGGGGAAGACACAGGTTGTTACTCCAATAGCACCTTTAAGCCAGCAGGCCGGATACTTTTGTTCAGTTTGTGAGTGCGTTGTTAAAGATTCTGCAAACTACTTGGATCATATCAATGGCAAAAAACATCAAAGAGCTTTGGGTATGTCTATGCGGGTAGAGCGGGCATCTCTTGAACAGGTCCAGAGTCGATTTGAAAAGCTCAAGAAGCGGAAAACTGATCTGCCCTTCACTGAGCAAGATCTTGATGAGCGGATCTTGAAGCTGCAGCAAGAAGAAGAGGAAAGGAAGCGACAACGTCGAGAAAAGAAGAAAGAGAAGAAGAAAGAGAAGGTAGATGAGGAGGAAATTGAAATTGACCCTGACGTTGCAGCAATGATGGGATTTGGTGGATTCGGTTCATCCAAGAAGTAA

Protein Analysis

203

Amino Acids

23.35

Weight (kDa)

9.19

Isoelectric Point (pI)

55.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
zf-met PF12874 84 - 108 3.1e-06 Zinc-finger of C2H2 type
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccBSI CCGCTC 2 cut(s) 358, 448
AccI GTMKAC 1 cut(s) 180
AccII CGCG 1 cut(s) 84
AciI CCGC 6 cut(s) 6, 82, 349, 358, 406, 448
AclWI GGATC 2 cut(s) 309, 458
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 50
AfaI GTAC 1 cut(s) 68
AgsI TTSAA 7 cut(s) 62, 160, 188, 371, 392, 457, 548
AluBI AGCT 5 cut(s) 23, 113, 332, 397, 460
AluI AGCT 5 cut(s) 23, 113, 332, 397, 460
Alw21I GWGCWC 1 cut(s) 115
AlwI GGATC 2 cut(s) 309, 458
AoxI GGCC 2 cut(s) 3, 243
ApeKI GCWGC 5 cut(s) 17, 20, 460, 463, 566
ApoI RAATTY 1 cut(s) 50
AspS9I GGNCC 2 cut(s) 134, 376
AvaII GGWCC 2 cut(s) 134, 376
BaeI ACNNNNGTAYC 2 cut(s) 241, 274
BanII GRGCYC 1 cut(s) 115
BbsI GAAGAC 1 cut(s) 209
Bbv12I GWGCWC 1 cut(s) 115
BbvI GCAGC 5 cut(s) 4, 32, 447, 475, 578
BccI CCATC 1 cut(s) 568
BceAI ACGGC 1 cut(s) 139
BciVI GTATCC 1 cut(s) 242
BfmI CTRYAG 2 cut(s) 18, 461
BfuI GTATCC 1 cut(s) 242
BglII AGATCT 1 cut(s) 436
BisI GCNGC 6 cut(s) 6, 18, 21, 461, 464, 567
BlsI GCNGC 6 cut(s) 7, 19, 22, 462, 465, 568
Bme18I GGWCC 2 cut(s) 134, 376
BmgT120I GGNCC 2 cut(s) 134, 376
BmsI GCATC 1 cut(s) 371
BpiI GAAGAC 1 cut(s) 209
BpmI CTGGAG 1 cut(s) 45
BpuEI CTTGAG 1 cut(s) 383
BsaWI WCCGGW 1 cut(s) 139
Bse3DI GCAATG 1 cut(s) 576
BseGI GGATG 1 cut(s) 596
BseMI GCAATG 1 cut(s) 576
BseMII CTCAG 2 cut(s) 63, 420
BseRI GAGGAG 2 cut(s) 119, 551
BseXI GCAGC 5 cut(s) 4, 32, 447, 475, 578
BsgI GTGCAG 1 cut(s) 164
Bsh1236I CGCG 1 cut(s) 84
BshFI GGCC 2 cut(s) 5, 245
BsiHKAI GWGCWC 1 cut(s) 115
BsiSI CCGG 2 cut(s) 140, 246
BsnI GGCC 2 cut(s) 5, 245
Bsp1286I GDGCHC 1 cut(s) 115
Bsp143I GATC 4 cut(s) 301, 415, 436, 450
BspACI CCGC 6 cut(s) 6, 82, 349, 358, 406, 448
BspANI GGCC 2 cut(s) 5, 245
BspCNI CTCAG 2 cut(s) 64, 421
BspFNI CGCG 1 cut(s) 84
BspMAI CTGCAG 2 cut(s) 22, 465
BspPI GGATC 2 cut(s) 309, 458
BsrBI CCGCTC 2 cut(s) 358, 448
BsrDI GCAATG 1 cut(s) 576
BssMI GATC 4 cut(s) 301, 415, 436, 450
Bst6I CTCTTC 3 cut(s) 57, 96, 468
BstC8I GCNNGC 5 cut(s) 82, 86, 239, 243, 360
BstDEI CTNAG 2 cut(s) 72, 429
BstF5I GGATG 1 cut(s) 596
BstFNI CGCG 1 cut(s) 84
BstKTI GATC 4 cut(s) 304, 418, 439, 453
BstMBI GATC 4 cut(s) 301, 415, 436, 450
BstMWI GCNNNNNNNGC 6 cut(s) 14, 77, 151, 160, 355, 403
BstSFI CTRYAG 2 cut(s) 18, 461
BstUI CGCG 1 cut(s) 84
BstV1I GCAGC 5 cut(s) 4, 32, 447, 475, 578
BstV2I GAAGAC 1 cut(s) 209
BstX2I RGATCY 2 cut(s) 436, 450
BstYI RGATCY 2 cut(s) 436, 450
BsuI GTATCC 1 cut(s) 242
BsuRI GGCC 2 cut(s) 5, 245
BtsCI GGATG 1 cut(s) 596
BtsIMutI CAGTG 1 cut(s) 426
Cac8I GCNNGC 5 cut(s) 82, 86, 239, 243, 360
Cfr13I GGNCC 2 cut(s) 134, 376
Csp6I GTAC 1 cut(s) 67
CviQI GTAC 1 cut(s) 67
DdeI CTNAG 2 cut(s) 72, 429
DpnI GATC 4 cut(s) 303, 417, 438, 452
DpnII GATC 4 cut(s) 301, 415, 436, 450
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 3 cut(s) 57, 96, 468
EarI CTCTTC 3 cut(s) 57, 96, 468
Ecl136II GAGCTC 1 cut(s) 113
Eco24I GRGCYC 1 cut(s) 115
Eco47I GGWCC 2 cut(s) 134, 376
Eco53kI GAGCTC 1 cut(s) 113
EcoICRI GAGCTC 1 cut(s) 113
EcoO109I RGGNCCY 1 cut(s) 134
EcoT38I GRGCYC 1 cut(s) 115
FaiI YATR 4 cut(s) 174, 306, 341, 347
FalI AAGNNNNNCTT 2 cut(s) 168, 200
FauI CCCGC 3 cut(s) 89, 342, 351
FblI GTMKAC 1 cut(s) 180
Fnu4HI GCNGC 6 cut(s) 6, 18, 21, 461, 464, 567
FokI GGATG 1 cut(s) 583
FriOI GRGCYC 1 cut(s) 115
Fsp4HI GCNGC 6 cut(s) 6, 18, 21, 461, 464, 567
GluI GCNGC 6 cut(s) 6, 18, 21, 461, 464, 567
GsuI CTGGAG 1 cut(s) 45
HaeIII GGCC 2 cut(s) 5, 245
HapII CCGG 2 cut(s) 140, 246
HinfI GANTC 4 cut(s) 188, 284, 382, 588
HpaII CCGG 2 cut(s) 140, 246
Hpy166II GTNNAC 1 cut(s) 181
Hpy188III TCNNGA 6 cut(s) 368, 379, 400, 440, 454, 497
Hpy8I GTNNAC 1 cut(s) 181
Hpy99I CGWCG 1 cut(s) 498
HpyAV CCTTC 3 cut(s) 39, 433, 520
HpyCH4IV ACGT 2 cut(s) 493, 561
HpyCH4V TGCA 5 cut(s) 20, 145, 290, 463, 566
HpyF10VI GCNNNNNNNGC 6 cut(s) 14, 77, 151, 160, 355, 403
HpyF3I CTNAG 2 cut(s) 72, 429
HpySE526I ACGT 2 cut(s) 493, 561
Kzo9I GATC 4 cut(s) 301, 415, 436, 450
LmnI GCTCC 1 cut(s) 110
Lsp1109I GCAGC 5 cut(s) 4, 32, 447, 475, 578
LweI GCATC 1 cut(s) 371
MaeII ACGT 2 cut(s) 493, 561
MaeIII GTNAC 1 cut(s) 214
MalI GATC 4 cut(s) 303, 417, 438, 452
MbiI CCGCTC 2 cut(s) 358, 448
MboI GATC 4 cut(s) 301, 415, 436, 450
MboII GAAGA 8 cut(s) 74, 113, 214, 482, 485, 517, 526, 529
MflI RGATCY 2 cut(s) 436, 450
MhlI GDGCHC 1 cut(s) 115
MluCI AATT 3 cut(s) 50, 543, 549
MlyI GAGTC 1 cut(s) 391
MnlI CCTC 7 cut(s) 97, 100, 141, 147, 469, 529, 532
MseI TTAA 2 cut(s) 233, 279
MslI CAYNNNNRTG 1 cut(s) 309
MspA1I CMGCKG 1 cut(s) 23
MspI CCGG 2 cut(s) 140, 246
MvnI CGCG 1 cut(s) 84
MwoI GCNNNNNNNGC 6 cut(s) 14, 77, 151, 160, 355, 403
NdeII GATC 4 cut(s) 301, 415, 436, 450
PfeI GAWTC 3 cut(s) 188, 284, 588
PflFI GACNNNGTC 1 cut(s) 492
PkrI GCNGC 6 cut(s) 7, 19, 22, 462, 465, 568
PleI GAGTC 1 cut(s) 390
PpsI GAGTC 1 cut(s) 390
PpuMI RGGWCCY 1 cut(s) 134
Psp124BI GAGCTC 1 cut(s) 115
Psp5II RGGWCCY 1 cut(s) 134
PspPI GGNCC 2 cut(s) 134, 376
PspPPI RGGWCCY 1 cut(s) 134
PstI CTGCAG 2 cut(s) 22, 465
PsuI RGATCY 2 cut(s) 436, 450
PsyI GACNNNGTC 1 cut(s) 492
PvuII CAGCTG 1 cut(s) 23
RsaI GTAC 1 cut(s) 68
RsaNI GTAC 1 cut(s) 67
RseI CAYNNNNRTG 1 cut(s) 309
SacI GAGCTC 1 cut(s) 115
SaqAI TTAA 2 cut(s) 233, 279
SatI GCNGC 6 cut(s) 6, 18, 21, 461, 464, 567
Sau3AI GATC 4 cut(s) 301, 415, 436, 450
Sau96I GGNCC 2 cut(s) 134, 376
SchI GAGTC 1 cut(s) 391
SduI GDGCHC 1 cut(s) 115
SfaNI GCATC 1 cut(s) 371
SfcI CTRYAG 2 cut(s) 18, 461
SinI GGWCC 2 cut(s) 134, 376
SmiMI CAYNNNNRTG 1 cut(s) 309
SmlI CTYRAG 1 cut(s) 398
SmoI CTYRAG 1 cut(s) 398
Sse9I AATT 3 cut(s) 50, 543, 549
SsiI CCGC 6 cut(s) 6, 82, 349, 358, 406, 448
SstI GAGCTC 1 cut(s) 115
TaiI ACGT 2 cut(s) 496, 564
TaqI TCGA 4 cut(s) 54, 122, 385, 496
TasI AATT 3 cut(s) 50, 543, 549
TauI GCSGC 1 cut(s) 8
TfiI GAWTC 3 cut(s) 188, 284, 588
Tru1I TTAA 2 cut(s) 233, 279
Tru9I TTAA 2 cut(s) 233, 279
TscAI CASTG 1 cut(s) 433
TseI GCWGC 5 cut(s) 17, 20, 460, 463, 566
TspDTI ATGAA 2 cut(s) 189, 585
TspRI CASTG 1 cut(s) 433
Tth111I GACNNNGTC 1 cut(s) 492
VpaK11BI GGWCC 2 cut(s) 134, 376
XapI RAATTY 1 cut(s) 50
XmiI GTMKAC 1 cut(s) 180
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.