FvH4_6g07820

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0005425
Physical Location & Seq
Forward (+)
4678583 .. 4679399
817 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g07820.t1

Sequence Viewer

Length: 486 bp
ATGTCAATTGTAACTGATGAGTTGAGAGCCAAGGCTGATGAGCTCTACCATGGAGATGCTATCTGCCAGGAGAAGTCGAAGTTCTTGCTCACAGAGATGGGTCTCCCCAACGGTCTCCTCCCCATGAAGGACATGGTGGAATGTGGCTACATCAAAGACACTGGCTTTGTTTGGCTCAAGCAGAAGAAGAGCACCACCCACAAGTTTGAGAAGGTTGGGAAGCTGGCCAGCTATGCAACTGAGATCACAGCCTCTGTTGAGAAGGGCAAGATCAAAAAGCTGACAGGAGTCAAGACCAAGGAGCTCATGATCTGGGTGTCAATCTGTGACATCTTTTTGGATGACCCCCCAACTGGGAAGATCACCTTCAAGTCTCCTACAGGGCTATTCAGGACATTTCCTGTCTCGGCTTTCGAGCTTGATGGACCAGATGGTGGCAAGACTGTGGAGGAACACCAGGACGTCCCGGAAGCTGTGGAAGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

162

Amino Acids

17.87

Weight (kDa)

5.68

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 139 4.6e-31 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 465
AccB7I CCANNNNNTGG 1 cut(s) 434
AcoI YGGCCR 1 cut(s) 225
AcyI GRCGYC 1 cut(s) 462
AfiI CCNNNNNNNGG 4 cut(s) 127, 353, 354, 434
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 2 cut(s) 66, 456
AluBI AGCT 7 cut(s) 43, 223, 231, 280, 304, 418, 473
AluI AGCT 7 cut(s) 43, 223, 231, 280, 304, 418, 473
Alw21I GWGCWC 3 cut(s) 45, 194, 306
Alw26I GTCTC 4 cut(s) 107, 119, 378, 409
AlwNI CAGNNNCTG 1 cut(s) 254
AoxI GGCC 1 cut(s) 225
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspS9I GGNCC 1 cut(s) 425
AsuC2I CCSGG 1 cut(s) 467
AsuHPI GGTGA 1 cut(s) 355
AvaII GGWCC 1 cut(s) 425
BalI TGGCCA 1 cut(s) 227
BanII GRGCYC 2 cut(s) 45, 306
Bbv12I GWGCWC 3 cut(s) 45, 194, 306
BccI CCATC 3 cut(s) 91, 416, 425
BcgI CGANNNNNNTGC 2 cut(s) 67, 101
BciT130I CCWGG 2 cut(s) 68, 458
BcnI CCSGG 1 cut(s) 467
BcoDI GTCTC 4 cut(s) 107, 119, 378, 409
BfaI CTAG 1 cut(s) 484
BfmI CTRYAG 1 cut(s) 378
Bme1390I CCNGG 3 cut(s) 68, 458, 467
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmrFI CCNGG 3 cut(s) 68, 458, 467
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 363
BoxI GACNNNNGTC 1 cut(s) 287
BpuEI CTTGAG 1 cut(s) 161
BpuMI CCSGG 1 cut(s) 467
BsaHI GRCGYC 1 cut(s) 462
BsaI GGTCTC 2 cut(s) 107, 119
BsaJI CCNNGG 3 cut(s) 30, 49, 297
Bsc4I CCNNNNNNNGG 4 cut(s) 127, 353, 354, 434
Bse1I ACTGG 2 cut(s) 166, 358
BseBI CCWGG 2 cut(s) 68, 458
BseDI CCNNGG 3 cut(s) 30, 49, 297
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 4 cut(s) 127, 353, 354, 434
BseMII CTCAG 1 cut(s) 231
BseNI ACTGG 2 cut(s) 166, 358
BseRI GAGGAG 1 cut(s) 107
BshFI GGCC 1 cut(s) 227
BsiHKAI GWGCWC 3 cut(s) 45, 194, 306
BsiSI CCGG 1 cut(s) 467
BslFI GGGAC 1 cut(s) 449
BslI CCNNNNNNNGG 4 cut(s) 127, 353, 354, 434
BsmAI GTCTC 4 cut(s) 107, 119, 378, 409
BsmFI GGGAC 1 cut(s) 449
BsnI GGCC 1 cut(s) 227
Bso31I GGTCTC 2 cut(s) 107, 119
Bsp1286I GDGCHC 3 cut(s) 45, 194, 306
Bsp143I GATC 4 cut(s) 243, 270, 309, 360
Bsp19I CCATGG 1 cut(s) 49
BspANI GGCC 1 cut(s) 227
BspCNI CTCAG 1 cut(s) 232
BspHI TCATGA 1 cut(s) 306
BspQI GCTCTTC 1 cut(s) 182
BspTNI GGTCTC 2 cut(s) 107, 119
BsrI ACTGG 2 cut(s) 166, 358
BssECI CCNNGG 3 cut(s) 30, 49, 297
BssMI GATC 4 cut(s) 243, 270, 309, 360
BssNI GRCGYC 1 cut(s) 462
BssT1I CCWWGG 3 cut(s) 30, 49, 297
Bst2UI CCWGG 2 cut(s) 68, 458
Bst4CI ACNGT 2 cut(s) 113, 445
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 462
BstC8I GCNNGC 2 cut(s) 225, 229
BstDEI CTNAG 1 cut(s) 240
BstDSI CCRYGG 1 cut(s) 49
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 4 cut(s) 246, 273, 312, 363
BstMAI GTCTC 4 cut(s) 107, 119, 378, 409
BstMBI GATC 4 cut(s) 243, 270, 309, 360
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 2 cut(s) 68, 458
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 3 cut(s) 66, 456, 465
BstSFI CTRYAG 1 cut(s) 378
BsuRI GGCC 1 cut(s) 227
BtgI CCRYGG 1 cut(s) 49
BtsCI GGATG 1 cut(s) 346
BtsIMutI CAGTG 1 cut(s) 159
Cac8I GCNNGC 2 cut(s) 225, 229
CaiI CAGNNNCTG 1 cut(s) 254
CciI TCATGA 1 cut(s) 306
Cfr13I GGNCC 1 cut(s) 425
CviAII CATG 4 cut(s) 50, 124, 133, 307
DdeI CTNAG 1 cut(s) 240
DpnI GATC 4 cut(s) 245, 272, 311, 362
DpnII GATC 4 cut(s) 243, 270, 309, 360
EaeI YGGCCR 1 cut(s) 225
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Ecl136II GAGCTC 2 cut(s) 43, 304
Eco130I CCWWGG 3 cut(s) 30, 49, 297
Eco24I GRGCYC 2 cut(s) 45, 306
Eco31I GGTCTC 2 cut(s) 107, 119
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 2 cut(s) 43, 304
EcoICRI GAGCTC 2 cut(s) 43, 304
EcoRII CCWGG 2 cut(s) 66, 456
EcoT14I CCWWGG 3 cut(s) 30, 49, 297
EcoT38I GRGCYC 2 cut(s) 45, 306
ErhI CCWWGG 3 cut(s) 30, 49, 297
FaeI CATG 4 cut(s) 53, 127, 136, 310
FaiI YATR 5 cut(s) 51, 125, 134, 234, 308
FalI AAGNNNNNCTT 2 cut(s) 350, 382
FaqI GGGAC 1 cut(s) 449
FatI CATG 4 cut(s) 49, 123, 132, 306
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 2 cut(s) 45, 306
FspBI CTAG 1 cut(s) 484
HaeIII GGCC 1 cut(s) 227
HapII CCGG 1 cut(s) 467
Hin1I GRCGYC 1 cut(s) 462
Hin1II CATG 4 cut(s) 53, 127, 136, 310
HinfI GANTC 1 cut(s) 288
HpaII CCGG 1 cut(s) 467
HphI GGTGA 1 cut(s) 355
Hpy188III TCNNGA 3 cut(s) 292, 307, 391
HpyAV CCTTC 4 cut(s) 121, 205, 256, 376
HpyCH4III ACNGT 2 cut(s) 113, 445
HpyCH4IV ACGT 1 cut(s) 462
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 1 cut(s) 240
HpySE526I ACGT 1 cut(s) 462
Hsp92I GRCGYC 1 cut(s) 462
Hsp92II CATG 4 cut(s) 53, 127, 136, 310
Kzo9I GATC 4 cut(s) 243, 270, 309, 360
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 1 cut(s) 301
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 484
MaeII ACGT 1 cut(s) 462
MaeIII GTNAC 2 cut(s) 10, 326
MalI GATC 4 cut(s) 245, 272, 311, 362
MboI GATC 4 cut(s) 243, 270, 309, 360
MboII GAAGA 3 cut(s) 196, 199, 370
MfeI CAATTG 1 cut(s) 6
MhlI GDGCHC 3 cut(s) 45, 194, 306
MlsI TGGCCA 1 cut(s) 227
MluCI AATT 1 cut(s) 6
MluNI TGGCCA 1 cut(s) 227
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 3 cut(s) 128, 262, 442
Mox20I TGGCCA 1 cut(s) 227
MscI TGGCCA 1 cut(s) 227
MslI CAYNNNNRTG 2 cut(s) 54, 95
Msp20I TGGCCA 1 cut(s) 227
MspI CCGG 1 cut(s) 467
MspR9I CCNGG 3 cut(s) 68, 458, 467
MunI CAATTG 1 cut(s) 6
MvaI CCWGG 2 cut(s) 68, 458
MwoI GCNNNNNNNGC 1 cut(s) 233
NciI CCSGG 1 cut(s) 467
NcoI CCATGG 1 cut(s) 49
NdeII GATC 4 cut(s) 243, 270, 309, 360
NlaIII CATG 4 cut(s) 53, 127, 136, 310
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 1 cut(s) 326
PagI TCATGA 1 cut(s) 306
PciSI GCTCTTC 1 cut(s) 182
PflMI CCANNNNNTGG 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 465
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PshAI GACNNNNGTC 1 cut(s) 287
Psp124BI GAGCTC 2 cut(s) 45, 306
Psp6I CCWGG 2 cut(s) 66, 456
PspGI CCWGG 2 cut(s) 66, 456
PspPI GGNCC 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 254
RseI CAYNNNNRTG 2 cut(s) 54, 95
SacI GAGCTC 2 cut(s) 45, 306
SapI GCTCTTC 1 cut(s) 182
Sau3AI GATC 4 cut(s) 243, 270, 309, 360
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 3 cut(s) 68, 458, 467
SduI GDGCHC 3 cut(s) 45, 194, 306
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 425
SmiMI CAYNNNNRTG 2 cut(s) 54, 95
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
Sse9I AATT 1 cut(s) 6
SspMI CTAG 1 cut(s) 484
SstI GAGCTC 2 cut(s) 45, 306
StyD4I CCNGG 3 cut(s) 66, 456, 465
StyI CCWWGG 3 cut(s) 30, 49, 297
TaaI ACNGT 2 cut(s) 113, 445
TaiI ACGT 1 cut(s) 465
TaqI TCGA 2 cut(s) 77, 414
TasI AATT 1 cut(s) 6
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 1 cut(s) 326
Tsp45I GTSAC 1 cut(s) 326
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 1 cut(s) 166
Van91I CCANNNNNTGG 1 cut(s) 434
VpaK11BI GGWCC 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 1 cut(s) 130
XspI CTAG 1 cut(s) 484
ZraI GACGTC 1 cut(s) 463
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.