Rorug03G0021100

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005425
Physical Location & Seq
Forward (+)
1715516 .. 1718752
3237 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0021100.1

Sequence Viewer

Length: 651 bp
ATGAATTTCCTCCTACCTTTTCGAGCTGATAAGCAACCACAATTACTATATAATGAACCAATTGTGGAAGCAACTTTCAATGACTTGGTCTTGTGCTGCTCAACCAAGCATTATCAACGTGACTACTACAACTGCAATCCACTCACCAAGCAATGGGTTGCTCCCCCTCCCGCCCCTCGAGCTCCCTATTCGAAAGTGCAGGTGGGATTCATTTGTGATCCCTACTATGATCATGGAATTAGTACCAGAAGTAGTAGTAGCTCTATGATGAAGCTTAATACTGGGTATAGGTGCAGGGTTGTGCGAATCCTCCCAGATGAAAGATTTCAACCAGGACTGGGTTTATATGTGGAGATCTTCTCTTCTGAGACAGGTGAATGGAGAGAGTATCAATTACCTAATCTAATAGAGTTAGGAAAGTTCGGTTGGTTCATTAAATGGCAGCCTGGCGTTGCTTTCAATGGCAAGTTATATTGGTACAATGATGGTGGCTTTACTTTTGAGTTGGATCCGTTCAATATTAGTAGTGGTGGTGATATTATTAATAACAAGCGTCGTTTCAATGTAGAACCGAGACTATGCATGCTTCCATTTATGTGTGTGCCAAGGGCGTTTGCGAATGTATCCATTCTCTATAGCGGGATATATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

216

Amino Acids

25.02

Weight (kDa)

8.42

Isoelectric Point (pI)

46.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
b-prop_At3g26010-like PF24750 22 - 162 7.8e-08 F-box protein At3g26010-like, beta-propeller
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 190
Acc36I ACCTGC 1 cut(s) 190
AccB7I CCANNNNNTGG 1 cut(s) 153
AciI CCGC 2 cut(s) 171, 639
AclWI GGATC 3 cut(s) 212, 503, 516
AcsI RAATTY 1 cut(s) 4
AfaI GTAC 2 cut(s) 244, 479
AfiI CCNNNNNNNGG 2 cut(s) 153, 338
AgsI TTSAA 5 cut(s) 79, 329, 460, 517, 562
AjnI CCWGG 2 cut(s) 331, 445
AjuI GAANNNNNNNTTGG 2 cut(s) 409, 441
AluBI AGCT 4 cut(s) 26, 182, 261, 274
AluI AGCT 4 cut(s) 26, 182, 261, 274
Alw21I GWGCWC 1 cut(s) 184
Alw26I GTCTC 2 cut(s) 362, 568
AlwI GGATC 3 cut(s) 212, 503, 516
Ama87I CYCGRG 1 cut(s) 177
ApeKI GCWGC 2 cut(s) 96, 442
ApoI RAATTY 1 cut(s) 4
AseI ATTAAT 1 cut(s) 543
Asp700I GAANNNNTTC 1 cut(s) 324
AsuHPI GGTGA 3 cut(s) 136, 386, 545
AsuII TTCGAA 1 cut(s) 191
AvaI CYCGRG 1 cut(s) 177
BamHI GGATCC 1 cut(s) 508
BanII GRGCYC 1 cut(s) 184
Bbv12I GWGCWC 1 cut(s) 184
BbvI GCAGC 2 cut(s) 83, 454
BccI CCATC 1 cut(s) 479
BciT130I CCWGG 2 cut(s) 333, 447
BciVI GTATCC 1 cut(s) 634
BclI TGATCA 1 cut(s) 229
BcoDI GTCTC 2 cut(s) 362, 568
BfmI CTRYAG 1 cut(s) 634
BfuAI ACCTGC 1 cut(s) 190
BfuI GTATCC 1 cut(s) 634
BglII AGATCT 1 cut(s) 354
BisI GCNGC 2 cut(s) 97, 443
BlsI GCNGC 2 cut(s) 98, 444
Bme1390I CCNGG 2 cut(s) 333, 447
BmeT110I CYCGRG 1 cut(s) 177
BmiI GGNNCC 1 cut(s) 510
BmrFI CCNGG 2 cut(s) 333, 447
BmrI ACTGGG 2 cut(s) 291, 347
BmuI ACTGGG 2 cut(s) 291, 347
BplI GAGNNNNNCTC 2 cut(s) 344, 376
Bpu14I TTCGAA 1 cut(s) 191
BsaJI CCNNGG 1 cut(s) 605
Bsc4I CCNNNNNNNGG 2 cut(s) 153, 338
Bse1I ACTGG 2 cut(s) 286, 342
Bse3DI GCAATG 1 cut(s) 158
BseBI CCWGG 2 cut(s) 333, 447
BseDI CCNNGG 1 cut(s) 605
BseLI CCNNNNNNNGG 2 cut(s) 153, 338
BseMI GCAATG 1 cut(s) 158
BseMII CTCAG 1 cut(s) 357
BseNI ACTGG 2 cut(s) 286, 342
BseXI GCAGC 2 cut(s) 83, 454
BsgI GTGCAG 2 cut(s) 218, 313
BsiHKAI GWGCWC 1 cut(s) 184
BsiHKCI CYCGRG 1 cut(s) 177
BslI CCNNNNNNNGG 2 cut(s) 153, 338
BsmAI GTCTC 2 cut(s) 362, 568
BsoBI CYCGRG 1 cut(s) 177
Bsp119I TTCGAA 1 cut(s) 191
Bsp1286I GDGCHC 1 cut(s) 184
Bsp143I GATC 4 cut(s) 217, 229, 354, 508
BspACI CCGC 2 cut(s) 171, 639
BspCNI CTCAG 1 cut(s) 358
BspLI GGNNCC 1 cut(s) 510
BspMI ACCTGC 1 cut(s) 190
BspPI GGATC 3 cut(s) 212, 503, 516
BspT104I TTCGAA 1 cut(s) 191
BsrDI GCAATG 1 cut(s) 158
BsrI ACTGG 2 cut(s) 286, 342
BssECI CCNNGG 1 cut(s) 605
BssMI GATC 4 cut(s) 217, 229, 354, 508
BssT1I CCWWGG 1 cut(s) 605
Bst2UI CCWGG 2 cut(s) 333, 447
Bst6I CTCTTC 1 cut(s) 367
BstBI TTCGAA 1 cut(s) 191
BstC8I GCNNGC 1 cut(s) 584
BstDEI CTNAG 1 cut(s) 366
BstKTI GATC 4 cut(s) 220, 232, 357, 511
BstMAI GTCTC 2 cut(s) 362, 568
BstMBI GATC 4 cut(s) 217, 229, 354, 508
BstMWI GCNNNNNNNGC 1 cut(s) 179
BstNI CCWGG 2 cut(s) 333, 447
BstNSI RCATGY 1 cut(s) 586
BstSCI CCNGG 2 cut(s) 331, 445
BstSFI CTRYAG 1 cut(s) 634
BstV1I GCAGC 2 cut(s) 83, 454
BstX2I RGATCY 2 cut(s) 354, 508
BstYI RGATCY 2 cut(s) 354, 508
BsuI GTATCC 1 cut(s) 634
BveI ACCTGC 1 cut(s) 190
Cac8I GCNNGC 1 cut(s) 584
CseI GACGC 1 cut(s) 542
Csp6I GTAC 2 cut(s) 243, 478
CspCI CAANNNNNGTGG 2 cut(s) 469, 504
CviAII CATG 2 cut(s) 233, 583
CviJI RGCY 6 cut(s) 26, 182, 261, 274, 445, 492
CviKI_1 RGCY 6 cut(s) 26, 182, 261, 274, 445, 492
CviQI GTAC 2 cut(s) 243, 478
DdeI CTNAG 1 cut(s) 366
DpnI GATC 4 cut(s) 219, 231, 356, 510
DpnII GATC 4 cut(s) 217, 229, 354, 508
Eam1104I CTCTTC 1 cut(s) 367
EarI CTCTTC 1 cut(s) 367
Ecl136II GAGCTC 1 cut(s) 182
Eco130I CCWWGG 1 cut(s) 605
Eco24I GRGCYC 1 cut(s) 184
Eco53kI GAGCTC 1 cut(s) 182
Eco88I CYCGRG 1 cut(s) 177
EcoICRI GAGCTC 1 cut(s) 182
EcoRII CCWGG 2 cut(s) 331, 445
EcoT14I CCWWGG 1 cut(s) 605
EcoT22I ATGCAT 1 cut(s) 584
EcoT38I GRGCYC 1 cut(s) 184
ErhI CCWWGG 1 cut(s) 605
FaeI CATG 2 cut(s) 236, 586
FatI CATG 2 cut(s) 232, 582
FauI CCCGC 2 cut(s) 178, 632
FbaI TGATCA 1 cut(s) 229
Fnu4HI GCNGC 2 cut(s) 97, 443
FriOI GRGCYC 1 cut(s) 184
Fsp4HI GCNGC 2 cut(s) 97, 443
GluI GCNGC 2 cut(s) 97, 443
HgaI GACGC 1 cut(s) 542
Hin1II CATG 2 cut(s) 236, 586
HindIII AAGCTT 1 cut(s) 272
HinfI GANTC 2 cut(s) 207, 306
HphI GGTGA 3 cut(s) 136, 386, 545
Hpy188I TCNGA 1 cut(s) 367
Hpy99I CGWCG 1 cut(s) 558
HpyCH4IV ACGT 1 cut(s) 118
HpyCH4V TGCA 4 cut(s) 135, 199, 294, 582
HpyF10VI GCNNNNNNNGC 1 cut(s) 179
HpyF3I CTNAG 1 cut(s) 366
HpySE526I ACGT 1 cut(s) 118
Hsp92II CATG 2 cut(s) 236, 586
Ksp22I TGATCA 1 cut(s) 229
Kzo9I GATC 4 cut(s) 217, 229, 354, 508
LmnI GCTCC 2 cut(s) 166, 187
Lsp1109I GCAGC 2 cut(s) 83, 454
MaeII ACGT 1 cut(s) 118
MaeIII GTNAC 1 cut(s) 119
MalI GATC 4 cut(s) 219, 231, 356, 510
MboI GATC 4 cut(s) 217, 229, 354, 508
MboII GAAGA 2 cut(s) 349, 354
MfeI CAATTG 1 cut(s) 60
MflI RGATCY 2 cut(s) 354, 508
MhlI GDGCHC 1 cut(s) 184
MluCI AATT 5 cut(s) 4, 41, 60, 237, 392
MmeI TCCRAC 1 cut(s) 486
MnlI CCTC 4 cut(s) 20, 177, 186, 320
Mph1103I ATGCAT 1 cut(s) 584
MroXI GAANNNNTTC 1 cut(s) 324
MseI TTAA 3 cut(s) 276, 435, 543
MslI CAYNNNNRTG 1 cut(s) 595
MspR9I CCNGG 2 cut(s) 333, 447
MunI CAATTG 1 cut(s) 60
MvaI CCWGG 2 cut(s) 333, 447
MwoI GCNNNNNNNGC 1 cut(s) 179
NdeII GATC 4 cut(s) 217, 229, 354, 508
NlaIII CATG 2 cut(s) 236, 586
NlaIV GGNNCC 1 cut(s) 510
NmuCI GTSAC 1 cut(s) 119
NsiI ATGCAT 1 cut(s) 584
NspI RCATGY 1 cut(s) 586
NspV TTCGAA 1 cut(s) 191
PaeI GCATGC 1 cut(s) 586
PaeR7I CTCGAG 1 cut(s) 177
PaqCI CACCTGC 1 cut(s) 190
PdmI GAANNNNTTC 1 cut(s) 324
PfeI GAWTC 2 cut(s) 207, 306
PflFI GACNNNGTC 1 cut(s) 86
PflMI CCANNNNNTGG 1 cut(s) 153
PkrI GCNGC 2 cut(s) 98, 444
PshBI ATTAAT 1 cut(s) 543
Psp124BI GAGCTC 1 cut(s) 184
Psp6I CCWGG 2 cut(s) 331, 445
PspGI CCWGG 2 cut(s) 331, 445
PspN4I GGNNCC 1 cut(s) 510
PspXI VCTCGAGB 1 cut(s) 177
PsuI RGATCY 2 cut(s) 354, 508
PsyI GACNNNGTC 1 cut(s) 86
RsaI GTAC 2 cut(s) 244, 479
RsaNI GTAC 2 cut(s) 243, 478
RseI CAYNNNNRTG 1 cut(s) 595
SacI GAGCTC 1 cut(s) 184
SaqAI TTAA 3 cut(s) 276, 435, 543
SatI GCNGC 2 cut(s) 97, 443
Sau3AI GATC 4 cut(s) 217, 229, 354, 508
ScrFI CCNGG 2 cut(s) 333, 447
SduI GDGCHC 1 cut(s) 184
SfcI CTRYAG 1 cut(s) 634
Sfr274I CTCGAG 1 cut(s) 177
SfuI TTCGAA 1 cut(s) 191
SlaI CTCGAG 1 cut(s) 177
SmiMI CAYNNNNRTG 1 cut(s) 595
SmlI CTYRAG 1 cut(s) 177
SmoI CTYRAG 1 cut(s) 177
SphI GCATGC 1 cut(s) 586
Sse9I AATT 5 cut(s) 4, 41, 60, 237, 392
SsiI CCGC 2 cut(s) 171, 639
SspI AATATT 1 cut(s) 520
SstI GAGCTC 1 cut(s) 184
StyD4I CCNGG 2 cut(s) 331, 445
StyI CCWWGG 1 cut(s) 605
TaiI ACGT 1 cut(s) 121
TaqI TCGA 3 cut(s) 22, 178, 191
TasI AATT 5 cut(s) 4, 41, 60, 237, 392
TfiI GAWTC 2 cut(s) 207, 306
Tru1I TTAA 3 cut(s) 276, 435, 543
Tru9I TTAA 3 cut(s) 276, 435, 543
TseFI GTSAC 1 cut(s) 119
TseI GCWGC 2 cut(s) 96, 442
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 6 cut(s) 17, 69, 199, 284, 333, 421
TspGWI ACGGA 1 cut(s) 501
Tth111I GACNNNGTC 1 cut(s) 86
Van91I CCANNNNNTGG 1 cut(s) 153
VspI ATTAAT 1 cut(s) 543
XapI RAATTY 1 cut(s) 4
XceI RCATGY 1 cut(s) 586
XhoI CTCGAG 1 cut(s) 177
XmnI GAANNNNTTC 1 cut(s) 324
Zsp2I ATGCAT 1 cut(s) 584
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.