MD04G1191700.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0005425
Physical Location & Seq
Reverse (-)
28102824 .. 28109449
6626 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1191700.v1.1.491

Sequence Viewer

Length: 540 bp
ATGTCCTCTCTCATCACAGCACAGATCAAGTCCAAAGCAGATGAGCTCTACCATGGAGATGAAATCTGCCAACAAAAAGCAAGGCAGCTTCTCTCAGAAATCTCCCTCCCCAATGGCCTCTTGCCCCTCAAGGACGTTGAGGAGTGTGGGATTATCAGAGAAACTGGCTTTGTGTGGCTCAAGCAGAAGAAGAGCTACACTCACAAGTATGAGAAAATTGGGAAGTTGAAGAAGAGCTACACCCACAAGTTTGAGAAAATTGGGAAGTTGGTCAGTTATGCCCCTGAGGTCACAGCCATTGTTGAGAAGGGCAAGATCAAGAAGCTGACAGGGGTGAAGACCAAGGAGCTCTTGGTGTGGGTGCAGCTCAGTGACATTTACGTTGATGACCCTCCAACTGGGAAGATCACCTTCAAGACCCCTGCTGGGTTGTTTAGGACTTTTCCTGTAAGTGCTTTTGAGGTTGAGGAAAGTGCTTGTGAGAAGGATGGGAAGGGGAAGGAGGTGAAGGAGGAGGTCAAGGGAGTTGTGGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

180

Amino Acids

20.12

Weight (kDa)

8.93

Isoelectric Point (pI)

27.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 154 4.1e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 2 cut(s) 398, 426
AgsI TTSAA 2 cut(s) 229, 415
AluBI AGCT 7 cut(s) 46, 88, 195, 237, 325, 349, 367
AluI AGCT 7 cut(s) 46, 88, 195, 237, 325, 349, 367
Alw21I GWGCWC 2 cut(s) 48, 351
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 2 cut(s) 85, 364
AsuHPI GGTGA 3 cut(s) 346, 400, 517
AxyI CCTNAGG 1 cut(s) 285
BanII GRGCYC 2 cut(s) 48, 351
BarI GAAGNNNNNNTAC 4 cut(s) 179, 211, 221, 253
BbsI GAAGAC 1 cut(s) 344
Bbv12I GWGCWC 2 cut(s) 48, 351
BbvI GCAGC 2 cut(s) 97, 376
BccI CCATC 1 cut(s) 482
BisI GCNGC 2 cut(s) 86, 365
BlsI GCNGC 2 cut(s) 87, 366
BmrI ACTGGG 1 cut(s) 408
BmuI ACTGGG 1 cut(s) 408
BpiI GAAGAC 1 cut(s) 344
BplI GAGNNNNNCTC 2 cut(s) 184, 216
BpuEI CTTGAG 2 cut(s) 113, 164
BsaJI CCNNGG 2 cut(s) 52, 342
Bsc4I CCNNNNNNNGG 2 cut(s) 398, 426
Bse1I ACTGG 2 cut(s) 169, 403
Bse21I CCTNAGG 1 cut(s) 285
BseDI CCNNGG 2 cut(s) 52, 342
BseGI GGATG 1 cut(s) 493
BseLI CCNNNNNNNGG 2 cut(s) 398, 426
BseMII CTCAG 3 cut(s) 108, 276, 382
BseNI ACTGG 2 cut(s) 169, 403
BseRI GAGGAG 2 cut(s) 155, 527
BseXI GCAGC 2 cut(s) 97, 376
BseYI CCCAGC 1 cut(s) 425
BsgI GTGCAG 1 cut(s) 383
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 48, 351
BslI CCNNNNNNNGG 2 cut(s) 398, 426
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 48, 351
Bsp143I GATC 3 cut(s) 24, 315, 405
Bsp19I CCATGG 1 cut(s) 52
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 107, 277, 381
BspQI GCTCTTC 2 cut(s) 185, 227
BsrI ACTGG 2 cut(s) 169, 403
BssECI CCNNGG 2 cut(s) 52, 342
BssMI GATC 3 cut(s) 24, 315, 405
BssT1I CCWWGG 2 cut(s) 52, 342
Bst6I CTCTTC 2 cut(s) 185, 227
BstDEI CTNAG 3 cut(s) 94, 285, 368
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 493
BstKTI GATC 3 cut(s) 27, 318, 408
BstMBI GATC 3 cut(s) 24, 315, 405
BstV1I GCAGC 2 cut(s) 97, 376
BstV2I GAAGAC 1 cut(s) 344
Bsu36I CCTNAGG 1 cut(s) 285
BsuRI GGCC 1 cut(s) 117
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 493
BtsIMutI CAGTG 1 cut(s) 376
CviAII CATG 1 cut(s) 53
DdeI CTNAG 3 cut(s) 94, 285, 368
DpnI GATC 3 cut(s) 26, 317, 407
DpnII GATC 3 cut(s) 24, 315, 405
Eam1104I CTCTTC 2 cut(s) 185, 227
EarI CTCTTC 2 cut(s) 185, 227
Ecl136II GAGCTC 2 cut(s) 46, 349
Eco130I CCWWGG 2 cut(s) 52, 342
Eco24I GRGCYC 2 cut(s) 48, 351
Eco53kI GAGCTC 2 cut(s) 46, 349
Eco81I CCTNAGG 1 cut(s) 285
EcoICRI GAGCTC 2 cut(s) 46, 349
EcoT14I CCWWGG 2 cut(s) 52, 342
EcoT38I GRGCYC 2 cut(s) 48, 351
ErhI CCWWGG 2 cut(s) 52, 342
FaeI CATG 1 cut(s) 56
FaiI YATR 3 cut(s) 54, 210, 279
FalI AAGNNNNNCTT 4 cut(s) 335, 367, 395, 427
FatI CATG 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 86, 365
FokI GGATG 1 cut(s) 500
FriOI GRGCYC 2 cut(s) 48, 351
Fsp4HI GCNGC 2 cut(s) 86, 365
GluI GCNGC 2 cut(s) 86, 365
GsaI CCCAGC 1 cut(s) 429
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 1 cut(s) 56
HphI GGTGA 3 cut(s) 346, 400, 517
Hpy188I TCNGA 2 cut(s) 97, 158
Hpy188III TCNNGA 2 cut(s) 319, 415
HpyAV CCTTC 6 cut(s) 301, 421, 478, 487, 493, 502
HpyCH4IV ACGT 2 cut(s) 135, 381
HpyCH4V TGCA 1 cut(s) 364
HpyF3I CTNAG 3 cut(s) 94, 285, 368
HpySE526I ACGT 2 cut(s) 135, 381
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 3 cut(s) 24, 315, 405
LguI GCTCTTC 2 cut(s) 185, 227
LmnI GCTCC 1 cut(s) 346
LpnPI CCDG 7 cut(s) 150, 297, 315, 384, 411, 435, 459
Lsp1109I GCAGC 2 cut(s) 97, 376
MaeII ACGT 2 cut(s) 135, 381
MaeIII GTNAC 2 cut(s) 289, 371
MalI GATC 3 cut(s) 26, 317, 407
MboI GATC 3 cut(s) 24, 315, 405
MboII GAAGA 6 cut(s) 199, 202, 241, 244, 349, 415
MhlI GDGCHC 2 cut(s) 48, 351
MluCI AATT 2 cut(s) 216, 258
MmeI TCCRAC 1 cut(s) 419
MslI CAYNNNNRTG 2 cut(s) 57, 207
NcoI CCATGG 1 cut(s) 52
NdeII GATC 3 cut(s) 24, 315, 405
NlaIII CATG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 289, 371
PciSI GCTCTTC 2 cut(s) 185, 227
PkrI GCNGC 2 cut(s) 87, 366
Psp124BI GAGCTC 2 cut(s) 48, 351
PspFI CCCAGC 1 cut(s) 425
RseI CAYNNNNRTG 2 cut(s) 57, 207
SacI GAGCTC 2 cut(s) 48, 351
SapI GCTCTTC 2 cut(s) 185, 227
SatI GCNGC 2 cut(s) 86, 365
Sau3AI GATC 3 cut(s) 24, 315, 405
SduI GDGCHC 2 cut(s) 48, 351
SmiMI CAYNNNNRTG 2 cut(s) 57, 207
SmlI CTYRAG 2 cut(s) 128, 179
SmoI CTYRAG 2 cut(s) 128, 179
Sse9I AATT 2 cut(s) 216, 258
SstI GAGCTC 2 cut(s) 48, 351
StyI CCWWGG 2 cut(s) 52, 342
TaiI ACGT 2 cut(s) 138, 384
TasI AATT 2 cut(s) 216, 258
TscAI CASTG 1 cut(s) 376
TseFI GTSAC 2 cut(s) 289, 371
TseI GCWGC 2 cut(s) 85, 364
Tsp45I GTSAC 2 cut(s) 289, 371
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 376
XcmI CCANNNNNNNNNTGG 1 cut(s) 349
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.