pycom12g19090

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr12
OG0005425
Physical Location & Seq
Reverse (-)
21044418 .. 21044918
501 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom12g19090.1

Sequence Viewer

Length: 501 bp
ATGTCTTCTCTCATCACAGCAGGGATCAAGTCCAAGGTAGATGAACTGTACCATGGAGATGAAATCTGCCAACAGAAATCAAAGGAGCTTCTCTCAGAAATCTCCCTCCCCAACGGCCTTTTGCCCCTCAAGGACATTGAGGAGTGCGGGATCGTGAGAGAAACCGGCTTTGTGTGGCTCAAGCAAAAGAAGAGCTGCACCCACAAGTTTGAGAAGATTGGCAAGTTGGTCACCTATGCCCCTGAGGTCACGGCCATTGTTGAGAAGGGCAAGATCAAGAAGCTGACAGGGGTGAAGACCAAGGAGCTTTTGGTTTGGGTGCAGCCGAGTGACATTTACACTGATGACCCTCCAAGTGGGAAGATCACCTTCAAGACCCCTTCTGGTTTGTTTAGGACTTTTCCTGTAAGTGCTTTTGAGGTTGAGGGAAGTGCTTGTGGGAAGGATGGGAAGGAGAAGAAGGAGGTGAAGGAGGAGGCCAAGGGAGTTGTGGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

167

Amino Acids

18.33

Weight (kDa)

8.24

Isoelectric Point (pI)

36.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 28 - 140 6.9e-33 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 147
AclWI GGATC 2 cut(s) 32, 158
AcoI YGGCCR 1 cut(s) 252
AfaI GTAC 1 cut(s) 50
AfiI CCNNNNNNNGG 1 cut(s) 356
AgsI TTSAA 1 cut(s) 373
AluBI AGCT 4 cut(s) 88, 195, 283, 307
AluI AGCT 4 cut(s) 88, 195, 283, 307
AlwI GGATC 2 cut(s) 32, 158
AoxI GGCC 3 cut(s) 115, 252, 477
ApeKI GCWGC 2 cut(s) 195, 322
AsuHPI GGTGA 4 cut(s) 223, 304, 358, 478
AxyI CCTNAGG 1 cut(s) 243
BbsI GAAGAC 1 cut(s) 302
BbvI GCAGC 2 cut(s) 182, 334
BccI CCATC 1 cut(s) 440
BceAI ACGGC 2 cut(s) 130, 267
BisI GCNGC 2 cut(s) 196, 323
BlsI GCNGC 2 cut(s) 197, 324
BpiI GAAGAC 1 cut(s) 302
BplI GAGNNNNNCTC 2 cut(s) 77, 109
BpuEI CTTGAG 2 cut(s) 113, 164
BsaJI CCNNGG 4 cut(s) 33, 52, 300, 480
Bsc4I CCNNNNNNNGG 1 cut(s) 356
Bse118I RCCGGY 1 cut(s) 164
Bse21I CCTNAGG 1 cut(s) 243
BseDI CCNNGG 4 cut(s) 33, 52, 300, 480
BseGI GGATG 1 cut(s) 451
BseLI CCNNNNNNNGG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 108, 234
BseRI GAGGAG 2 cut(s) 155, 488
BseXI GCAGC 2 cut(s) 182, 334
BsgI GTGCAG 2 cut(s) 181, 341
BshFI GGCC 3 cut(s) 117, 254, 479
BsiSI CCGG 1 cut(s) 165
BslI CCNNNNNNNGG 1 cut(s) 356
BsnI GGCC 3 cut(s) 117, 254, 479
Bsp143I GATC 4 cut(s) 24, 150, 273, 363
Bsp19I CCATGG 1 cut(s) 52
BspACI CCGC 1 cut(s) 147
BspANI GGCC 3 cut(s) 117, 254, 479
BspCNI CTCAG 2 cut(s) 107, 235
BspPI GGATC 2 cut(s) 32, 158
BspQI GCTCTTC 1 cut(s) 185
BsrFI RCCGGY 1 cut(s) 164
BssAI RCCGGY 1 cut(s) 164
BssECI CCNNGG 4 cut(s) 33, 52, 300, 480
BssMI GATC 4 cut(s) 24, 150, 273, 363
BssT1I CCWWGG 4 cut(s) 33, 52, 300, 480
Bst4CI ACNGT 1 cut(s) 48
Bst6I CTCTTC 1 cut(s) 185
BstDEI CTNAG 2 cut(s) 94, 243
BstDSI CCRYGG 1 cut(s) 52
BstEII GGTNACC 1 cut(s) 229
BstF5I GGATG 1 cut(s) 451
BstKTI GATC 4 cut(s) 27, 153, 276, 366
BstMBI GATC 4 cut(s) 24, 150, 273, 363
BstPI GGTNACC 1 cut(s) 229
BstV1I GCAGC 2 cut(s) 182, 334
BstV2I GAAGAC 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 3 cut(s) 117, 254, 479
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 451
BtsIMutI CAGTG 1 cut(s) 339
Cfr10I RCCGGY 1 cut(s) 164
Csp6I GTAC 1 cut(s) 49
CviAII CATG 1 cut(s) 53
CviQI GTAC 1 cut(s) 49
DdeI CTNAG 2 cut(s) 94, 243
DpnI GATC 4 cut(s) 26, 152, 275, 365
DpnII GATC 4 cut(s) 24, 150, 273, 363
EaeI YGGCCR 1 cut(s) 252
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Eco130I CCWWGG 4 cut(s) 33, 52, 300, 480
Eco81I CCTNAGG 1 cut(s) 243
Eco91I GGTNACC 1 cut(s) 229
EcoO65I GGTNACC 1 cut(s) 229
EcoT14I CCWWGG 4 cut(s) 33, 52, 300, 480
ErhI CCWWGG 4 cut(s) 33, 52, 300, 480
FaeI CATG 1 cut(s) 56
FaiI YATR 2 cut(s) 54, 237
FalI AAGNNNNNCTT 2 cut(s) 353, 385
FatI CATG 1 cut(s) 52
FauI CCCGC 1 cut(s) 140
Fnu4HI GCNGC 2 cut(s) 196, 323
FokI GGATG 1 cut(s) 458
Fsp4HI GCNGC 2 cut(s) 196, 323
GluI GCNGC 2 cut(s) 196, 323
HaeIII GGCC 3 cut(s) 117, 254, 479
HapII CCGG 1 cut(s) 165
Hin1II CATG 1 cut(s) 56
HpaII CCGG 1 cut(s) 165
HphI GGTGA 4 cut(s) 223, 304, 358, 478
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 3 cut(s) 154, 277, 373
HpyAV CCTTC 7 cut(s) 259, 379, 390, 436, 445, 454, 463
HpyCH4III ACNGT 1 cut(s) 48
HpyCH4V TGCA 2 cut(s) 198, 322
HpyF3I CTNAG 2 cut(s) 94, 243
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 4 cut(s) 24, 150, 273, 363
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 2 cut(s) 85, 304
LpnPI CCDG 6 cut(s) 6, 178, 255, 273, 369, 417
Lsp1109I GCAGC 2 cut(s) 182, 334
MaeIII GTNAC 3 cut(s) 229, 247, 329
MalI GATC 4 cut(s) 26, 152, 275, 365
MboI GATC 4 cut(s) 24, 150, 273, 363
MboII GAAGA 5 cut(s) 202, 226, 307, 373, 469
MslI CAYNNNNRTG 1 cut(s) 57
MspI CCGG 1 cut(s) 165
NcoI CCATGG 1 cut(s) 52
NdeII GATC 4 cut(s) 24, 150, 273, 363
NlaIII CATG 1 cut(s) 56
NmeAIII GCCGAG 1 cut(s) 351
NmuCI GTSAC 3 cut(s) 229, 247, 329
PciSI GCTCTTC 1 cut(s) 185
PkrI GCNGC 2 cut(s) 197, 324
PspEI GGTNACC 1 cut(s) 229
RsaI GTAC 1 cut(s) 50
RsaNI GTAC 1 cut(s) 49
RseI CAYNNNNRTG 1 cut(s) 57
SapI GCTCTTC 1 cut(s) 185
SatI GCNGC 2 cut(s) 196, 323
Sau3AI GATC 4 cut(s) 24, 150, 273, 363
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 2 cut(s) 128, 179
SmoI CTYRAG 2 cut(s) 128, 179
SsiI CCGC 1 cut(s) 147
StyI CCWWGG 4 cut(s) 33, 52, 300, 480
TaaI ACNGT 1 cut(s) 48
TscAI CASTG 1 cut(s) 346
TseFI GTSAC 3 cut(s) 229, 247, 329
TseI GCWGC 2 cut(s) 195, 322
Tsp45I GTSAC 3 cut(s) 229, 247, 329
TspDTI ATGAA 2 cut(s) 57, 75
TspRI CASTG 1 cut(s) 346
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.