Rh3DG085300

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0005425
Physical Location & Seq
Forward (+)
6670198 .. 6671394
1197 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG085300.1

Sequence Viewer

Length: 606 bp
ATGAATCCCACCTCTCTCAACACAGGTCTCATCCTCACCTTTGTTCACACACTCGAGCACATTTCAAAAACCAAACATCATAAAAAGAGCACCAAAGTTTTCACAAGAATGTCTCTTATAACTGACGAGGTGAGAGCCAAGGCTGATGAGTTCTACCAAGGTGATGCTATCTGTCAGGAGAAGTCAAAGTTCTTGCTCACAGAGAAGGGTCTCCCCAATGGGCTTCTGCCCATGAAGGACATGGTGGAATGTGGCTACATCAAAGACACTGGCTTTGTTTGGCTCAAGCAGAAGAAGAGCACCACCCACAAGTTTGAGAAGGTTGGGAGGCTGGCGAGCTATGCAACTGAGATCACAGCCACTGTTGAGAAAGGCAAGATCAGTAAGCTGACTGGAGTCAAGACCAAGGAGCTCTTGGTCTGGGTGTCACTCAGTGACATCTATTTGGATGACCCCCCAACTGGAAAGATCACCTTCAAGTCTCCTACAGGGCTATTCAGGACATTTCCTGCCTCGGCTTTCGAGCTTGATGGACCTGATGGTGGCAAGGCAATGGAGGTCAATGAGGAGGAGGTCAAGGACGTCCCCGGAGCTGTGGAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

201

Amino Acids

22.33

Weight (kDa)

8.38

Isoelectric Point (pI)

27.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 65 - 175 3.2e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 119
AatII GACGTC 1 cut(s) 585
AcyI GRCGYC 1 cut(s) 582
AdeI CACNNNGTG 1 cut(s) 434
AfiI CCNNNNNNNGG 2 cut(s) 461, 542
AgsI TTSAA 2 cut(s) 66, 478
AluBI AGCT 5 cut(s) 339, 388, 412, 526, 593
AluI AGCT 5 cut(s) 339, 388, 412, 526, 593
Alw21I GWGCWC 4 cut(s) 60, 92, 302, 414
Alw26I GTCTC 4 cut(s) 32, 117, 215, 486
AlwNI CAGNNNCTG 1 cut(s) 362
Ama87I CYCGRG 1 cut(s) 53
ArsI GACNNNNNNTTYG 2 cut(s) 257, 289
AspS9I GGNCC 1 cut(s) 533
AsuC2I CCSGG 1 cut(s) 588
AsuHPI GGTGA 4 cut(s) 28, 142, 173, 463
AvaI CYCGRG 1 cut(s) 53
AvaII GGWCC 1 cut(s) 533
BanII GRGCYC 1 cut(s) 414
Bbv12I GWGCWC 4 cut(s) 60, 92, 302, 414
BccI CCATC 2 cut(s) 524, 533
BcnI CCSGG 1 cut(s) 588
BcoDI GTCTC 4 cut(s) 32, 117, 215, 486
BfaI CTAG 1 cut(s) 604
BfmI CTRYAG 1 cut(s) 486
Bme1390I CCNGG 1 cut(s) 588
Bme18I GGWCC 1 cut(s) 533
BmeT110I CYCGRG 1 cut(s) 53
BmgT120I GGNCC 1 cut(s) 533
BmrFI CCNGG 1 cut(s) 588
BmsI GCATC 1 cut(s) 154
BoxI GACNNNNGTC 1 cut(s) 395
BpmI CTGGAG 1 cut(s) 414
BpuEI CTTGAG 1 cut(s) 269
BpuMI CCSGG 1 cut(s) 588
BsaHI GRCGYC 1 cut(s) 582
BsaI GGTCTC 2 cut(s) 32, 215
BsaJI CCNNGG 5 cut(s) 138, 157, 405, 513, 586
Bsc4I CCNNNNNNNGG 2 cut(s) 461, 542
Bse1I ACTGG 3 cut(s) 274, 397, 466
Bse3DI GCAATG 1 cut(s) 558
BseDI CCNNGG 5 cut(s) 138, 157, 405, 513, 586
BseGI GGATG 2 cut(s) 30, 454
BseLI CCNNNNNNNGG 2 cut(s) 461, 542
BseMI GCAATG 1 cut(s) 558
BseMII CTCAG 2 cut(s) 339, 445
BseNI ACTGG 3 cut(s) 274, 397, 466
BseRI GAGGAG 2 cut(s) 581, 584
BsiHKAI GWGCWC 4 cut(s) 60, 92, 302, 414
BsiHKCI CYCGRG 1 cut(s) 53
BsiSI CCGG 1 cut(s) 588
BslFI GGGAC 1 cut(s) 569
BslI CCNNNNNNNGG 2 cut(s) 461, 542
BsmAI GTCTC 4 cut(s) 32, 117, 215, 486
BsmFI GGGAC 1 cut(s) 569
Bso31I GGTCTC 2 cut(s) 32, 215
BsoBI CYCGRG 1 cut(s) 53
Bsp1286I GDGCHC 4 cut(s) 60, 92, 302, 414
Bsp143I GATC 3 cut(s) 351, 378, 468
BspCNI CTCAG 2 cut(s) 340, 444
BspQI GCTCTTC 1 cut(s) 290
BspTNI GGTCTC 2 cut(s) 32, 215
BsrDI GCAATG 1 cut(s) 558
BsrI ACTGG 3 cut(s) 274, 397, 466
BssECI CCNNGG 5 cut(s) 138, 157, 405, 513, 586
BssMI GATC 3 cut(s) 351, 378, 468
BssNI GRCGYC 1 cut(s) 582
BssT1I CCWWGG 3 cut(s) 138, 157, 405
Bst4CI ACNGT 1 cut(s) 364
Bst6I CTCTTC 1 cut(s) 290
BstACI GRCGYC 1 cut(s) 582
BstC8I GCNNGC 2 cut(s) 333, 337
BstDEI CTNAG 2 cut(s) 348, 431
BstF5I GGATG 2 cut(s) 30, 454
BstKTI GATC 3 cut(s) 354, 381, 471
BstMAI GTCTC 4 cut(s) 32, 117, 215, 486
BstMBI GATC 3 cut(s) 351, 378, 468
BstMWI GCNNNNNNNGC 1 cut(s) 341
BstPAI GACNNNNGTC 1 cut(s) 395
BstSCI CCNGG 1 cut(s) 586
BstSFI CTRYAG 1 cut(s) 486
BtsCI GGATG 2 cut(s) 30, 454
BtsIMutI CAGTG 3 cut(s) 267, 360, 439
Cac8I GCNNGC 2 cut(s) 333, 337
CaiI CAGNNNCTG 1 cut(s) 362
Cfr13I GGNCC 1 cut(s) 533
CviAII CATG 2 cut(s) 232, 241
DdeI CTNAG 2 cut(s) 348, 431
DpnI GATC 3 cut(s) 353, 380, 470
DpnII GATC 3 cut(s) 351, 378, 468
DraIII CACNNNGTG 1 cut(s) 434
Eam1104I CTCTTC 1 cut(s) 290
EarI CTCTTC 1 cut(s) 290
Ecl136II GAGCTC 1 cut(s) 412
Eco130I CCWWGG 3 cut(s) 138, 157, 405
Eco24I GRGCYC 1 cut(s) 414
Eco31I GGTCTC 2 cut(s) 32, 215
Eco47I GGWCC 1 cut(s) 533
Eco53kI GAGCTC 1 cut(s) 412
Eco88I CYCGRG 1 cut(s) 53
EcoICRI GAGCTC 1 cut(s) 412
EcoT14I CCWWGG 3 cut(s) 138, 157, 405
EcoT38I GRGCYC 1 cut(s) 414
ErhI CCWWGG 3 cut(s) 138, 157, 405
FaeI CATG 2 cut(s) 235, 244
FaiI YATR 5 cut(s) 81, 119, 233, 242, 342
FalI AAGNNNNNCTT 4 cut(s) 398, 430, 458, 490
FaqI GGGAC 1 cut(s) 569
FatI CATG 2 cut(s) 231, 240
FokI GGATG 2 cut(s) 17, 461
FriOI GRGCYC 1 cut(s) 414
FspBI CTAG 1 cut(s) 604
GsuI CTGGAG 1 cut(s) 414
HapII CCGG 1 cut(s) 588
Hin1I GRCGYC 1 cut(s) 582
Hin1II CATG 2 cut(s) 235, 244
HinfI GANTC 2 cut(s) 4, 396
HpaII CCGG 1 cut(s) 588
HphI GGTGA 4 cut(s) 28, 142, 173, 463
Hpy166II GTNNAC 1 cut(s) 46
Hpy188III TCNNGA 3 cut(s) 176, 400, 499
Hpy8I GTNNAC 1 cut(s) 46
HpyAV CCTTC 4 cut(s) 199, 229, 313, 484
HpyCH4III ACNGT 1 cut(s) 364
HpyCH4IV ACGT 1 cut(s) 582
HpyCH4V TGCA 1 cut(s) 344
HpyF10VI GCNNNNNNNGC 1 cut(s) 341
HpyF3I CTNAG 2 cut(s) 348, 431
HpySE526I ACGT 1 cut(s) 582
Hsp92I GRCGYC 1 cut(s) 582
Hsp92II CATG 2 cut(s) 235, 244
Kzo9I GATC 3 cut(s) 351, 378, 468
LguI GCTCTTC 1 cut(s) 290
LmnI GCTCC 2 cut(s) 409, 590
LweI GCATC 1 cut(s) 154
MaeI CTAG 1 cut(s) 604
MaeII ACGT 1 cut(s) 582
MaeIII GTNAC 2 cut(s) 426, 434
MalI GATC 3 cut(s) 353, 380, 470
MboI GATC 3 cut(s) 351, 378, 468
MboII GAAGA 2 cut(s) 304, 307
MhlI GDGCHC 4 cut(s) 60, 92, 302, 414
MlyI GAGTC 1 cut(s) 405
MslI CAYNNNNRTG 1 cut(s) 107
MspI CCGG 1 cut(s) 588
MspR9I CCNGG 1 cut(s) 588
MwoI GCNNNNNNNGC 1 cut(s) 341
NciI CCSGG 1 cut(s) 588
NdeII GATC 3 cut(s) 351, 378, 468
NlaIII CATG 2 cut(s) 235, 244
NmeAIII GCCGAG 1 cut(s) 494
NmuCI GTSAC 2 cut(s) 426, 434
PaeR7I CTCGAG 1 cut(s) 53
PciSI GCTCTTC 1 cut(s) 290
PfeI GAWTC 1 cut(s) 4
PleI GAGTC 1 cut(s) 404
PpsI GAGTC 1 cut(s) 404
PshAI GACNNNNGTC 1 cut(s) 395
PsiI TTATAA 1 cut(s) 119
Psp124BI GAGCTC 1 cut(s) 414
PspPI GGNCC 1 cut(s) 533
PspXI VCTCGAGB 1 cut(s) 53
PstNI CAGNNNCTG 1 cut(s) 362
RseI CAYNNNNRTG 1 cut(s) 107
SacI GAGCTC 1 cut(s) 414
SapI GCTCTTC 1 cut(s) 290
Sau3AI GATC 3 cut(s) 351, 378, 468
Sau96I GGNCC 1 cut(s) 533
SchI GAGTC 1 cut(s) 405
ScrFI CCNGG 1 cut(s) 588
SduI GDGCHC 4 cut(s) 60, 92, 302, 414
SfaNI GCATC 1 cut(s) 154
SfcI CTRYAG 1 cut(s) 486
Sfr274I CTCGAG 1 cut(s) 53
SinI GGWCC 1 cut(s) 533
SlaI CTCGAG 1 cut(s) 53
SmiMI CAYNNNNRTG 1 cut(s) 107
SmlI CTYRAG 2 cut(s) 53, 284
SmoI CTYRAG 2 cut(s) 53, 284
SspMI CTAG 1 cut(s) 604
SstI GAGCTC 1 cut(s) 414
StyD4I CCNGG 1 cut(s) 586
StyI CCWWGG 3 cut(s) 138, 157, 405
TaaI ACNGT 1 cut(s) 364
TaiI ACGT 1 cut(s) 585
TaqI TCGA 2 cut(s) 54, 522
TfiI GAWTC 1 cut(s) 4
TscAI CASTG 3 cut(s) 274, 367, 439
TseFI GTSAC 2 cut(s) 426, 434
Tsp45I GTSAC 2 cut(s) 426, 434
TspDTI ATGAA 2 cut(s) 17, 248
TspRI CASTG 3 cut(s) 274, 367, 439
VpaK11BI GGWCC 1 cut(s) 533
XcmI CCANNNNNNNNNTGG 2 cut(s) 238, 412
XhoI CTCGAG 1 cut(s) 53
XspI CTAG 1 cut(s) 604
ZraI GACGTC 1 cut(s) 583
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.