Rorug03G0020800

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0005425
Physical Location & Seq
Reverse (-)
1682113 .. 1683476
1364 bp
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UTR
Exon/CDS
Intron
Rorug03G0020800.1

Sequence Viewer

Length: 402 bp
ATGGCTATGAGTCACGCGAGATCGTTGAGGAGCGTTATCAATTCCATGGAATTGGAAGCAGTAGGGGTGTTAGTTTCAGGGCGACGAGCATTTTCTGCTAAGGCAAAGAAGGGTGGCAAAGGTGGTGCTGGTGATGCACCGAAGGCGTCAATTTTAGACAAGGAAGTTAAGTCTACGACTGTGGTTGGAGCTAATATACTCAAGGATGGGACGGATCCTAAAATCTTGCCAGATTCCGAGTACCCTGAATGGCTTTGGCATCTGATTGATAAGCGTCCTGCGTTGAGTGAACTGAGAAGGAAGAACATTGAAACTCTCCCCTATGAAGATCTCAAGCGATTTGTTAAGCTGGATAACCGGGCAAGGATCAAGGAGAACAACTCGCTAAAGGCCAAGAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

133

Amino Acids

14.72

Weight (kDa)

9.9

Isoelectric Point (pI)

60.13

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L37 PF08561 53 - 130 1.4e-23 Mitochondrial ribosomal protein L37
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 173
AccII CGCG 1 cut(s) 17
AclWI GGATC 3 cut(s) 209, 222, 374
AcyI GRCGYC 1 cut(s) 146
AfaI GTAC 1 cut(s) 242
AgsI TTSAA 1 cut(s) 311
AluBI AGCT 2 cut(s) 191, 349
AluI AGCT 2 cut(s) 191, 349
AlwI GGATC 3 cut(s) 209, 222, 374
AoxI GGCC 1 cut(s) 390
AsuC2I CCSGG 1 cut(s) 359
AsuHPI GGTGA 1 cut(s) 143
BamHI GGATCC 1 cut(s) 214
BccI CCATC 1 cut(s) 200
BcnI CCSGG 1 cut(s) 359
BfaI CTAG 1 cut(s) 400
BglII AGATCT 1 cut(s) 328
Bme1390I CCNGG 1 cut(s) 359
BmiI GGNNCC 1 cut(s) 216
BmrFI CCNGG 1 cut(s) 359
BmsI GCATC 2 cut(s) 124, 268
BplI GAGNNNNNCTC 2 cut(s) 365, 397
Bpu10I CCTNAGC 1 cut(s) 99
BpuEI CTTGAG 2 cut(s) 185, 317
BpuMI CCSGG 1 cut(s) 359
BsaHI GRCGYC 1 cut(s) 146
BsaJI CCNNGG 1 cut(s) 45
BseDI CCNNGG 1 cut(s) 45
BseGI GGATG 1 cut(s) 211
BseMII CTCAG 1 cut(s) 284
BseRI GAGGAG 1 cut(s) 43
Bsh1236I CGCG 1 cut(s) 17
BshFI GGCC 1 cut(s) 392
BsiSI CCGG 1 cut(s) 358
BslFI GGGAC 1 cut(s) 223
BsmFI GGGAC 1 cut(s) 223
BsnI GGCC 1 cut(s) 392
Bsp143I GATC 4 cut(s) 20, 214, 328, 366
Bsp19I CCATGG 1 cut(s) 45
BspANI GGCC 1 cut(s) 392
BspCNI CTCAG 1 cut(s) 285
BspFNI CGCG 1 cut(s) 17
BspLI GGNNCC 1 cut(s) 216
BspPI GGATC 3 cut(s) 209, 222, 374
BssECI CCNNGG 1 cut(s) 45
BssMI GATC 4 cut(s) 20, 214, 328, 366
BssNI GRCGYC 1 cut(s) 146
BssT1I CCWWGG 1 cut(s) 45
Bst4CI ACNGT 1 cut(s) 181
BstACI GRCGYC 1 cut(s) 146
BstAPI GCANNNNNTGC 1 cut(s) 95
BstDEI CTNAG 2 cut(s) 99, 293
BstDSI CCRYGG 1 cut(s) 45
BstF5I GGATG 1 cut(s) 211
BstFNI CGCG 1 cut(s) 17
BstKTI GATC 4 cut(s) 23, 217, 331, 369
BstMBI GATC 4 cut(s) 20, 214, 328, 366
BstMWI GCNNNNNNNGC 3 cut(s) 95, 134, 143
BstSCI CCNGG 1 cut(s) 357
BstUI CGCG 1 cut(s) 17
BstX2I RGATCY 2 cut(s) 214, 328
BstXI CCANNNNNNTGG 1 cut(s) 52
BstYI RGATCY 2 cut(s) 214, 328
BsuRI GGCC 1 cut(s) 392
BtgI CCRYGG 1 cut(s) 45
BtsCI GGATG 1 cut(s) 211
CseI GACGC 2 cut(s) 135, 263
Csp6I GTAC 1 cut(s) 241
CviAII CATG 1 cut(s) 46
CviJI RGCY 5 cut(s) 5, 191, 253, 349, 392
CviKI_1 RGCY 5 cut(s) 5, 191, 253, 349, 392
CviQI GTAC 1 cut(s) 241
DdeI CTNAG 2 cut(s) 99, 293
DpnI GATC 4 cut(s) 22, 216, 330, 368
DpnII GATC 4 cut(s) 20, 214, 328, 366
Eco130I CCWWGG 1 cut(s) 45
EcoT14I CCWWGG 1 cut(s) 45
ErhI CCWWGG 1 cut(s) 45
FaeI CATG 1 cut(s) 49
FaiI YATR 4 cut(s) 8, 47, 197, 324
FaqI GGGAC 1 cut(s) 223
FatI CATG 1 cut(s) 45
FblI GTMKAC 1 cut(s) 173
FokI GGATG 1 cut(s) 218
FspBI CTAG 1 cut(s) 400
HaeIII GGCC 1 cut(s) 392
HapII CCGG 1 cut(s) 358
HgaI GACGC 2 cut(s) 135, 263
Hin1I GRCGYC 1 cut(s) 146
Hin1II CATG 1 cut(s) 49
HinfI GANTC 2 cut(s) 10, 233
HpaII CCGG 1 cut(s) 358
HphI GGTGA 1 cut(s) 143
Hpy166II GTNNAC 2 cut(s) 174, 290
Hpy188I TCNGA 2 cut(s) 238, 264
Hpy8I GTNNAC 2 cut(s) 174, 290
Hpy99I CGWCG 1 cut(s) 87
HpyAV CCTTC 3 cut(s) 103, 136, 291
HpyCH4III ACNGT 1 cut(s) 181
HpyCH4V TGCA 1 cut(s) 137
HpyF10VI GCNNNNNNNGC 3 cut(s) 95, 134, 143
HpyF3I CTNAG 2 cut(s) 99, 293
Hsp92I GRCGYC 1 cut(s) 146
Hsp92II CATG 1 cut(s) 49
Kzo9I GATC 4 cut(s) 20, 214, 328, 366
LmnI GCTCC 2 cut(s) 30, 188
LpnPI CCDG 7 cut(s) 63, 114, 243, 258, 291, 335, 371
LweI GCATC 2 cut(s) 124, 268
MaeI CTAG 1 cut(s) 400
MaeIII GTNAC 1 cut(s) 11
MalI GATC 4 cut(s) 22, 216, 330, 368
MboI GATC 4 cut(s) 20, 214, 328, 366
MboII GAAGA 2 cut(s) 313, 338
MflI RGATCY 2 cut(s) 214, 328
MluCI AATT 3 cut(s) 40, 50, 150
MlyI GAGTC 1 cut(s) 19
MmeI TCCRAC 1 cut(s) 166
MnlI CCTC 1 cut(s) 21
MseI TTAA 2 cut(s) 168, 345
MspI CCGG 1 cut(s) 358
MspR9I CCNGG 1 cut(s) 359
MvnI CGCG 1 cut(s) 17
MwoI GCNNNNNNNGC 3 cut(s) 95, 134, 143
NciI CCSGG 1 cut(s) 359
NcoI CCATGG 1 cut(s) 45
NdeII GATC 4 cut(s) 20, 214, 328, 366
NlaIII CATG 1 cut(s) 49
NlaIV GGNNCC 1 cut(s) 216
NmuCI GTSAC 1 cut(s) 11
PfeI GAWTC 1 cut(s) 233
PleI GAGTC 1 cut(s) 18
PpsI GAGTC 1 cut(s) 18
PspN4I GGNNCC 1 cut(s) 216
PsuI RGATCY 2 cut(s) 214, 328
RsaI GTAC 1 cut(s) 242
RsaNI GTAC 1 cut(s) 241
SaqAI TTAA 2 cut(s) 168, 345
Sau3AI GATC 4 cut(s) 20, 214, 328, 366
SchI GAGTC 1 cut(s) 19
ScrFI CCNGG 1 cut(s) 359
SetI ASST 3 cut(s) 124, 193, 351
SfaNI GCATC 2 cut(s) 124, 268
SmlI CTYRAG 2 cut(s) 200, 332
SmoI CTYRAG 2 cut(s) 200, 332
Sse9I AATT 3 cut(s) 40, 50, 150
SspMI CTAG 1 cut(s) 400
StyD4I CCNGG 1 cut(s) 357
StyI CCWWGG 1 cut(s) 45
TaaI ACNGT 1 cut(s) 181
TasI AATT 3 cut(s) 40, 50, 150
TfiI GAWTC 1 cut(s) 233
Tru1I TTAA 2 cut(s) 168, 345
Tru9I TTAA 2 cut(s) 168, 345
TseFI GTSAC 1 cut(s) 11
Tsp45I GTSAC 1 cut(s) 11
TspDTI ATGAA 1 cut(s) 339
TspGWI ACGGA 1 cut(s) 227
XmiI GTMKAC 1 cut(s) 173
XspI CTAG 1 cut(s) 400
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.