MD13G1256800.v1.1

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr13
OG0005425
Physical Location & Seq
Forward (+)
28526122 .. 28526487
366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD13G1256800.v1.1.491

Sequence Viewer

Length: 366 bp
ATGTCCTCTCTCATCACAGCAGAGATCAAATCCAAAGCAGATGAGCTCTACCATGAAGATGAAATCTGCCAACAAAAAACAAAGCAACTTTTCTCAGAAATCTCCCTCCCCAATGGCCTCTTGCCCCTCAAGCAAAAGAAGAGCTACACTCACAAGTTTGAGAAAATTGGCAAGTTGGTCATTTATGCCCCTGAGGTCACAACCATTGTTGAGAAGGGCAAGATCAAGAAGTTGACAGGGGTGAAGACCAAGGAGCTCTTGGTGTGGGTGCAGCTCAGTAACATTTACGTTGATGACCCTCAAACTGGGAAAGTCACCTTCAAGACCCCTAATGGGTTATTTAGGACTTTTCCTTTTAAATTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

122

Amino Acids

13.86

Weight (kDa)

9.47

Isoelectric Point (pI)

29.09

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 44 - 119 1.6e-16 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AcsI RAATTY 1 cut(s) 359
AfiI CCNNNNNNNGG 2 cut(s) 305, 333
AgsI TTSAA 1 cut(s) 322
AluBI AGCT 4 cut(s) 46, 144, 256, 274
AluI AGCT 4 cut(s) 46, 144, 256, 274
Alw21I GWGCWC 2 cut(s) 48, 258
AoxI GGCC 1 cut(s) 115
ApeKI GCWGC 1 cut(s) 271
ApoI RAATTY 1 cut(s) 359
AsuHPI GGTGA 2 cut(s) 253, 307
AxyI CCTNAGG 1 cut(s) 192
BanII GRGCYC 2 cut(s) 48, 258
BbsI GAAGAC 1 cut(s) 251
Bbv12I GWGCWC 2 cut(s) 48, 258
BbvI GCAGC 1 cut(s) 283
BisI GCNGC 1 cut(s) 272
BlsI GCNGC 1 cut(s) 273
BmrI ACTGGG 1 cut(s) 315
BmuI ACTGGG 1 cut(s) 315
BpiI GAAGAC 1 cut(s) 251
BplI GAGNNNNNCTC 2 cut(s) 133, 165
BpuEI CTTGAG 1 cut(s) 113
BsaJI CCNNGG 1 cut(s) 249
Bsc4I CCNNNNNNNGG 2 cut(s) 305, 333
Bse1I ACTGG 1 cut(s) 310
Bse21I CCTNAGG 1 cut(s) 192
BseDI CCNNGG 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 305, 333
BseMII CTCAG 3 cut(s) 108, 183, 289
BseNI ACTGG 1 cut(s) 310
BseXI GCAGC 1 cut(s) 283
BsgI GTGCAG 1 cut(s) 290
BshFI GGCC 1 cut(s) 117
BsiHKAI GWGCWC 2 cut(s) 48, 258
BslI CCNNNNNNNGG 2 cut(s) 305, 333
BsnI GGCC 1 cut(s) 117
Bsp1286I GDGCHC 2 cut(s) 48, 258
Bsp143I GATC 2 cut(s) 24, 222
BspANI GGCC 1 cut(s) 117
BspCNI CTCAG 3 cut(s) 107, 184, 288
BspQI GCTCTTC 1 cut(s) 134
BsrI ACTGG 1 cut(s) 310
BssECI CCNNGG 1 cut(s) 249
BssMI GATC 2 cut(s) 24, 222
BssT1I CCWWGG 1 cut(s) 249
Bst6I CTCTTC 1 cut(s) 134
BstDEI CTNAG 3 cut(s) 94, 192, 275
BstKTI GATC 2 cut(s) 27, 225
BstMBI GATC 2 cut(s) 24, 222
BstMWI GCNNNNNNNGC 1 cut(s) 130
BstV1I GCAGC 1 cut(s) 283
BstV2I GAAGAC 1 cut(s) 251
Bsu36I CCTNAGG 1 cut(s) 192
BsuRI GGCC 1 cut(s) 117
CviAII CATG 1 cut(s) 53
CviJI RGCY 5 cut(s) 46, 117, 144, 256, 274
CviKI_1 RGCY 5 cut(s) 46, 117, 144, 256, 274
DdeI CTNAG 3 cut(s) 94, 192, 275
DpnI GATC 2 cut(s) 26, 224
DpnII GATC 2 cut(s) 24, 222
DraI TTTAAA 1 cut(s) 358
Eam1104I CTCTTC 1 cut(s) 134
EarI CTCTTC 1 cut(s) 134
Ecl136II GAGCTC 2 cut(s) 46, 256
Eco130I CCWWGG 1 cut(s) 249
Eco24I GRGCYC 2 cut(s) 48, 258
Eco53kI GAGCTC 2 cut(s) 46, 256
Eco81I CCTNAGG 1 cut(s) 192
EcoICRI GAGCTC 2 cut(s) 46, 256
EcoT14I CCWWGG 1 cut(s) 249
EcoT38I GRGCYC 2 cut(s) 48, 258
ErhI CCWWGG 1 cut(s) 249
FaeI CATG 1 cut(s) 56
FaiI YATR 2 cut(s) 54, 186
FalI AAGNNNNNCTT 2 cut(s) 242, 274
FatI CATG 1 cut(s) 52
Fnu4HI GCNGC 1 cut(s) 272
FriOI GRGCYC 2 cut(s) 48, 258
Fsp4HI GCNGC 1 cut(s) 272
GluI GCNGC 1 cut(s) 272
HaeIII GGCC 1 cut(s) 117
Hin1II CATG 1 cut(s) 56
HincII GTYRAC 1 cut(s) 234
HindII GTYRAC 1 cut(s) 234
HphI GGTGA 2 cut(s) 253, 307
Hpy166II GTNNAC 1 cut(s) 234
Hpy188I TCNGA 1 cut(s) 97
Hpy188III TCNNGA 2 cut(s) 226, 322
Hpy8I GTNNAC 1 cut(s) 234
HpyAV CCTTC 2 cut(s) 208, 328
HpyCH4IV ACGT 1 cut(s) 288
HpyCH4V TGCA 1 cut(s) 271
HpyF10VI GCNNNNNNNGC 1 cut(s) 130
HpyF3I CTNAG 3 cut(s) 94, 192, 275
HpySE526I ACGT 1 cut(s) 288
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 2 cut(s) 24, 222
LguI GCTCTTC 1 cut(s) 134
LmnI GCTCC 1 cut(s) 253
LpnPI CCDG 3 cut(s) 204, 222, 291
Lsp1109I GCAGC 1 cut(s) 283
MaeII ACGT 1 cut(s) 288
MaeIII GTNAC 3 cut(s) 196, 278, 313
MalI GATC 2 cut(s) 26, 224
MboI GATC 2 cut(s) 24, 222
MboII GAAGA 3 cut(s) 68, 151, 256
MhlI GDGCHC 2 cut(s) 48, 258
MluCI AATT 2 cut(s) 165, 359
MnlI CCTC 6 cut(s) 16, 116, 128, 137, 187, 309
MseI TTAA 1 cut(s) 357
MslI CAYNNNNRTG 1 cut(s) 57
MwoI GCNNNNNNNGC 1 cut(s) 130
NdeII GATC 2 cut(s) 24, 222
NlaIII CATG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 196, 313
PciSI GCTCTTC 1 cut(s) 134
PkrI GCNGC 1 cut(s) 273
Psp124BI GAGCTC 2 cut(s) 48, 258
RseI CAYNNNNRTG 1 cut(s) 57
SacI GAGCTC 2 cut(s) 48, 258
SapI GCTCTTC 1 cut(s) 134
SaqAI TTAA 1 cut(s) 357
SatI GCNGC 1 cut(s) 272
Sau3AI GATC 2 cut(s) 24, 222
SduI GDGCHC 2 cut(s) 48, 258
SetI ASST 7 cut(s) 48, 146, 198, 258, 276, 291, 320
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 1 cut(s) 128
SmoI CTYRAG 1 cut(s) 128
Sse9I AATT 2 cut(s) 165, 359
SstI GAGCTC 2 cut(s) 48, 258
StyI CCWWGG 1 cut(s) 249
TaiI ACGT 1 cut(s) 291
TasI AATT 2 cut(s) 165, 359
Tru1I TTAA 1 cut(s) 357
Tru9I TTAA 1 cut(s) 357
TseFI GTSAC 2 cut(s) 196, 313
TseI GCWGC 1 cut(s) 271
Tsp45I GTSAC 2 cut(s) 196, 313
TspDTI ATGAA 2 cut(s) 69, 75
XapI RAATTY 1 cut(s) 359
XcmI CCANNNNNNNNNTGG 1 cut(s) 256
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.