Rroxscaffold_6G00421790

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000006
OG0005425
Physical Location & Seq
Reverse (-)
42855402 .. 42855899
498 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_6G00421790.1

Sequence Viewer

Length: 498 bp
ATGTCTCTTATAACTGACGAGTTGAGAGCCAAGGCTGATGAGTTCTACCAAGGTGATGCTATCTGCCAGGAGAAGTCAAAGTTCTTGCTCACAGAGAAGGGTCTCCCCAATGGGCTTCTGCCCATGAAGGACATGGTGGAATGTGGCTACATCAAAGACACTGGCTTTGTTTGGCTCAAGCAGAAGAAGAGCACCACCCACAAGTTTGAGAAGGTTGGGAGGCTGGCGAGCTATGCAACTGAGATCACAGCCACTGTTGAGAAAGGCAAGATCAGTAAGCTGACTGGAGTCAAGACCAAGGAGCTCTTGGTCTGGGTGTCACTCAGTGACATCTATTTGGATGACCCCCCAACTGGGAAGATCACCTTCAAGTCTCCTACAGGGCTATTCAGGACATTTCCTGCCTCGGCTTTCGAGCTTGATGGACCTGATGGTGGCAAGGCAATGGAGGTCAATGAGGAGGAGGTCAAGGACGTCCTCGGAGCTGTGGAGGTCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

165

Amino Acids

18.23

Weight (kDa)

5.35

Isoelectric Point (pI)

28.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 139 1.8e-30 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 11
AatII GACGTC 1 cut(s) 477
AcyI GRCGYC 1 cut(s) 474
AdeI CACNNNGTG 1 cut(s) 326
AfiI CCNNNNNNNGG 3 cut(s) 353, 354, 434
AgsI TTSAA 1 cut(s) 370
AjnI CCWGG 1 cut(s) 66
AluBI AGCT 5 cut(s) 231, 280, 304, 418, 485
AluI AGCT 5 cut(s) 231, 280, 304, 418, 485
Alw21I GWGCWC 2 cut(s) 194, 306
Alw26I GTCTC 3 cut(s) 9, 107, 378
AlwNI CAGNNNCTG 1 cut(s) 254
ArsI GACNNNNNNTTYG 2 cut(s) 149, 181
AspS9I GGNCC 1 cut(s) 425
AsuHPI GGTGA 2 cut(s) 65, 355
AvaII GGWCC 1 cut(s) 425
BanII GRGCYC 1 cut(s) 306
Bbv12I GWGCWC 2 cut(s) 194, 306
BccI CCATC 2 cut(s) 416, 425
BciT130I CCWGG 1 cut(s) 68
BcoDI GTCTC 3 cut(s) 9, 107, 378
BfaI CTAG 1 cut(s) 496
BfmI CTRYAG 1 cut(s) 378
Bme1390I CCNGG 1 cut(s) 68
Bme18I GGWCC 1 cut(s) 425
BmgT120I GGNCC 1 cut(s) 425
BmrFI CCNGG 1 cut(s) 68
BmrI ACTGGG 1 cut(s) 363
BmsI GCATC 1 cut(s) 46
BmuI ACTGGG 1 cut(s) 363
BoxI GACNNNNGTC 1 cut(s) 287
BpmI CTGGAG 1 cut(s) 306
BpuEI CTTGAG 1 cut(s) 161
BsaHI GRCGYC 1 cut(s) 474
BsaI GGTCTC 1 cut(s) 107
BsaJI CCNNGG 5 cut(s) 30, 49, 297, 405, 478
Bsc4I CCNNNNNNNGG 3 cut(s) 353, 354, 434
Bse1I ACTGG 3 cut(s) 166, 289, 358
Bse3DI GCAATG 1 cut(s) 450
BseBI CCWGG 1 cut(s) 68
BseDI CCNNGG 5 cut(s) 30, 49, 297, 405, 478
BseGI GGATG 1 cut(s) 346
BseLI CCNNNNNNNGG 3 cut(s) 353, 354, 434
BseMI GCAATG 1 cut(s) 450
BseMII CTCAG 2 cut(s) 231, 337
BseNI ACTGG 3 cut(s) 166, 289, 358
BseRI GAGGAG 2 cut(s) 473, 476
BsiHKAI GWGCWC 2 cut(s) 194, 306
BslI CCNNNNNNNGG 3 cut(s) 353, 354, 434
BsmAI GTCTC 3 cut(s) 9, 107, 378
Bso31I GGTCTC 1 cut(s) 107
Bsp1286I GDGCHC 2 cut(s) 194, 306
Bsp143I GATC 3 cut(s) 243, 270, 360
BspCNI CTCAG 2 cut(s) 232, 336
BspQI GCTCTTC 1 cut(s) 182
BspTNI GGTCTC 1 cut(s) 107
BsrDI GCAATG 1 cut(s) 450
BsrI ACTGG 3 cut(s) 166, 289, 358
BssECI CCNNGG 5 cut(s) 30, 49, 297, 405, 478
BssMI GATC 3 cut(s) 243, 270, 360
BssNI GRCGYC 1 cut(s) 474
BssT1I CCWWGG 3 cut(s) 30, 49, 297
Bst2UI CCWGG 1 cut(s) 68
Bst4CI ACNGT 1 cut(s) 256
Bst6I CTCTTC 1 cut(s) 182
BstACI GRCGYC 1 cut(s) 474
BstC8I GCNNGC 2 cut(s) 225, 229
BstDEI CTNAG 2 cut(s) 240, 323
BstF5I GGATG 1 cut(s) 346
BstKTI GATC 3 cut(s) 246, 273, 363
BstMAI GTCTC 3 cut(s) 9, 107, 378
BstMBI GATC 3 cut(s) 243, 270, 360
BstMWI GCNNNNNNNGC 1 cut(s) 233
BstNI CCWGG 1 cut(s) 68
BstPAI GACNNNNGTC 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 66
BstSFI CTRYAG 1 cut(s) 378
BtsCI GGATG 1 cut(s) 346
BtsIMutI CAGTG 3 cut(s) 159, 252, 331
Cac8I GCNNGC 2 cut(s) 225, 229
CaiI CAGNNNCTG 1 cut(s) 254
Cfr13I GGNCC 1 cut(s) 425
CviAII CATG 2 cut(s) 124, 133
DdeI CTNAG 2 cut(s) 240, 323
DpnI GATC 3 cut(s) 245, 272, 362
DpnII GATC 3 cut(s) 243, 270, 360
DraIII CACNNNGTG 1 cut(s) 326
Eam1104I CTCTTC 1 cut(s) 182
EarI CTCTTC 1 cut(s) 182
Ecl136II GAGCTC 1 cut(s) 304
Eco130I CCWWGG 3 cut(s) 30, 49, 297
Eco24I GRGCYC 1 cut(s) 306
Eco31I GGTCTC 1 cut(s) 107
Eco47I GGWCC 1 cut(s) 425
Eco53kI GAGCTC 1 cut(s) 304
EcoICRI GAGCTC 1 cut(s) 304
EcoRII CCWGG 1 cut(s) 66
EcoT14I CCWWGG 3 cut(s) 30, 49, 297
EcoT38I GRGCYC 1 cut(s) 306
ErhI CCWWGG 3 cut(s) 30, 49, 297
FaeI CATG 2 cut(s) 127, 136
FaiI YATR 4 cut(s) 11, 125, 134, 234
FalI AAGNNNNNCTT 4 cut(s) 290, 322, 350, 382
FatI CATG 2 cut(s) 123, 132
FokI GGATG 1 cut(s) 353
FriOI GRGCYC 1 cut(s) 306
FspBI CTAG 1 cut(s) 496
GsuI CTGGAG 1 cut(s) 306
Hin1I GRCGYC 1 cut(s) 474
Hin1II CATG 2 cut(s) 127, 136
HinfI GANTC 1 cut(s) 288
HphI GGTGA 2 cut(s) 65, 355
Hpy188I TCNGA 1 cut(s) 482
Hpy188III TCNNGA 2 cut(s) 292, 391
HpyAV CCTTC 4 cut(s) 91, 121, 205, 376
HpyCH4III ACNGT 1 cut(s) 256
HpyCH4IV ACGT 1 cut(s) 474
HpyCH4V TGCA 1 cut(s) 236
HpyF10VI GCNNNNNNNGC 1 cut(s) 233
HpyF3I CTNAG 2 cut(s) 240, 323
HpySE526I ACGT 1 cut(s) 474
Hsp92I GRCGYC 1 cut(s) 474
Hsp92II CATG 2 cut(s) 127, 136
Kzo9I GATC 3 cut(s) 243, 270, 360
LguI GCTCTTC 1 cut(s) 182
LmnI GCTCC 2 cut(s) 301, 482
LweI GCATC 1 cut(s) 46
MaeI CTAG 1 cut(s) 496
MaeII ACGT 1 cut(s) 474
MaeIII GTNAC 2 cut(s) 318, 326
MalI GATC 3 cut(s) 245, 272, 362
MboI GATC 3 cut(s) 243, 270, 360
MboII GAAGA 3 cut(s) 196, 199, 370
MhlI GDGCHC 2 cut(s) 194, 306
MlyI GAGTC 1 cut(s) 297
MnlI CCTC 8 cut(s) 213, 415, 442, 451, 454, 457, 484, 488
MspR9I CCNGG 1 cut(s) 68
MvaI CCWGG 1 cut(s) 68
MwoI GCNNNNNNNGC 1 cut(s) 233
NdeII GATC 3 cut(s) 243, 270, 360
NlaIII CATG 2 cut(s) 127, 136
NmeAIII GCCGAG 1 cut(s) 386
NmuCI GTSAC 2 cut(s) 318, 326
PciSI GCTCTTC 1 cut(s) 182
PleI GAGTC 1 cut(s) 296
PpsI GAGTC 1 cut(s) 296
PshAI GACNNNNGTC 1 cut(s) 287
PsiI TTATAA 1 cut(s) 11
Psp124BI GAGCTC 1 cut(s) 306
Psp6I CCWGG 1 cut(s) 66
PspGI CCWGG 1 cut(s) 66
PspPI GGNCC 1 cut(s) 425
PstNI CAGNNNCTG 1 cut(s) 254
SacI GAGCTC 1 cut(s) 306
SapI GCTCTTC 1 cut(s) 182
Sau3AI GATC 3 cut(s) 243, 270, 360
Sau96I GGNCC 1 cut(s) 425
SchI GAGTC 1 cut(s) 297
ScrFI CCNGG 1 cut(s) 68
SduI GDGCHC 2 cut(s) 194, 306
SfaNI GCATC 1 cut(s) 46
SfcI CTRYAG 1 cut(s) 378
SinI GGWCC 1 cut(s) 425
SmlI CTYRAG 1 cut(s) 176
SmoI CTYRAG 1 cut(s) 176
SspMI CTAG 1 cut(s) 496
SstI GAGCTC 1 cut(s) 306
StyD4I CCNGG 1 cut(s) 66
StyI CCWWGG 3 cut(s) 30, 49, 297
TaaI ACNGT 1 cut(s) 256
TaiI ACGT 1 cut(s) 477
TaqI TCGA 1 cut(s) 414
TscAI CASTG 3 cut(s) 166, 259, 331
TseFI GTSAC 2 cut(s) 318, 326
Tsp45I GTSAC 2 cut(s) 318, 326
TspDTI ATGAA 1 cut(s) 140
TspRI CASTG 3 cut(s) 166, 259, 331
VpaK11BI GGWCC 1 cut(s) 425
XcmI CCANNNNNNNNNTGG 2 cut(s) 130, 304
XspI CTAG 1 cut(s) 496
ZraI GACGTC 1 cut(s) 475
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.