pycom04g16930

Protein of unknown function, DUF538

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr4
OG0005425
Physical Location & Seq
Reverse (-)
19474581 .. 19475082
502 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom04g16930.1

Sequence Viewer

Length: 462 bp
ATGTCTTCTCTCATCACAGCAGAGATCAAGTCCAAAGCAGATGAGCTCTACCATGGAGATGAAATCTGCCAACAAAAATCAAAGCAGCTTCTCTCAGAAATCTTCCTCCCCAATGGCCTCTTGCCCCTCAAGGACATTGAGGAGTGTGGGATTATCAGAGAAACTGGCTTTGTGTGGCTCAAGCAGAAGAAGAGCTACACCCACAAGTTTGAGAAAATTGGGAAGTTGGTCAGTTATGCCCCTGAGGTCACAGCCATTGTTGAGAAGGGCAAGATCAAGAAACTGACAGGGGTGAAGACCAAGGAGCTTCTGGTGTGGGTGCAGCTCAGTGACATTTACACCCCTGCTGGGTTGTTTAGGACTTTTCCTGTAAGTGCTTTTGAGGTTGAGGAAAGTGCTTCTGGGAAGGATGGGAAGGGGAAGGAGGTGAAGGAGGAGGCCAAGGGAGTTGTGGAGGTTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF538

Protein Analysis

154

Amino Acids

17.06

Weight (kDa)

7.69

Isoelectric Point (pI)

34.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF538 PF04398 29 - 127 1.9e-26 Protein of unknown function, DUF538
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AfiI CCNNNNNNNGG 1 cut(s) 348
AluBI AGCT 5 cut(s) 46, 88, 195, 307, 325
AluI AGCT 5 cut(s) 46, 88, 195, 307, 325
Alw21I GWGCWC 1 cut(s) 48
AoxI GGCC 2 cut(s) 115, 438
ApeKI GCWGC 2 cut(s) 85, 322
AsuHPI GGTGA 2 cut(s) 304, 439
AxyI CCTNAGG 1 cut(s) 243
BanII GRGCYC 1 cut(s) 48
BarI GAAGNNNNNNTAC 2 cut(s) 179, 211
BbsI GAAGAC 1 cut(s) 302
Bbv12I GWGCWC 1 cut(s) 48
BbvI GCAGC 2 cut(s) 97, 334
BccI CCATC 1 cut(s) 404
BisI GCNGC 2 cut(s) 86, 323
BlsI GCNGC 2 cut(s) 87, 324
BpiI GAAGAC 1 cut(s) 302
BpuEI CTTGAG 2 cut(s) 113, 164
BsaJI CCNNGG 3 cut(s) 52, 300, 441
Bsc4I CCNNNNNNNGG 1 cut(s) 348
Bse1I ACTGG 1 cut(s) 169
Bse21I CCTNAGG 1 cut(s) 243
BseDI CCNNGG 3 cut(s) 52, 300, 441
BseGI GGATG 1 cut(s) 415
BseLI CCNNNNNNNGG 1 cut(s) 348
BseMII CTCAG 3 cut(s) 108, 234, 340
BseNI ACTGG 1 cut(s) 169
BseRI GAGGAG 2 cut(s) 155, 449
BseXI GCAGC 2 cut(s) 97, 334
BseYI CCCAGC 1 cut(s) 347
BsgI GTGCAG 1 cut(s) 341
BshFI GGCC 2 cut(s) 117, 440
BsiHKAI GWGCWC 1 cut(s) 48
BslI CCNNNNNNNGG 1 cut(s) 348
BsnI GGCC 2 cut(s) 117, 440
Bsp1286I GDGCHC 1 cut(s) 48
Bsp143I GATC 2 cut(s) 24, 273
Bsp19I CCATGG 1 cut(s) 52
BspANI GGCC 2 cut(s) 117, 440
BspCNI CTCAG 3 cut(s) 107, 235, 339
BspQI GCTCTTC 1 cut(s) 185
BsrI ACTGG 1 cut(s) 169
BssECI CCNNGG 3 cut(s) 52, 300, 441
BssMI GATC 2 cut(s) 24, 273
BssT1I CCWWGG 3 cut(s) 52, 300, 441
Bst6I CTCTTC 1 cut(s) 185
BstDEI CTNAG 3 cut(s) 94, 243, 326
BstDSI CCRYGG 1 cut(s) 52
BstF5I GGATG 1 cut(s) 415
BstKTI GATC 2 cut(s) 27, 276
BstMBI GATC 2 cut(s) 24, 273
BstV1I GCAGC 2 cut(s) 97, 334
BstV2I GAAGAC 1 cut(s) 302
Bsu36I CCTNAGG 1 cut(s) 243
BsuRI GGCC 2 cut(s) 117, 440
BtgI CCRYGG 1 cut(s) 52
BtsCI GGATG 1 cut(s) 415
BtsIMutI CAGTG 1 cut(s) 334
CviAII CATG 1 cut(s) 53
DdeI CTNAG 3 cut(s) 94, 243, 326
DpnI GATC 2 cut(s) 26, 275
DpnII GATC 2 cut(s) 24, 273
Eam1104I CTCTTC 1 cut(s) 185
EarI CTCTTC 1 cut(s) 185
Ecl136II GAGCTC 1 cut(s) 46
Eco130I CCWWGG 3 cut(s) 52, 300, 441
Eco24I GRGCYC 1 cut(s) 48
Eco53kI GAGCTC 1 cut(s) 46
Eco81I CCTNAGG 1 cut(s) 243
EcoICRI GAGCTC 1 cut(s) 46
EcoT14I CCWWGG 3 cut(s) 52, 300, 441
EcoT38I GRGCYC 1 cut(s) 48
ErhI CCWWGG 3 cut(s) 52, 300, 441
FaeI CATG 1 cut(s) 56
FaiI YATR 2 cut(s) 54, 237
FatI CATG 1 cut(s) 52
Fnu4HI GCNGC 2 cut(s) 86, 323
FokI GGATG 1 cut(s) 422
FriOI GRGCYC 1 cut(s) 48
Fsp4HI GCNGC 2 cut(s) 86, 323
GluI GCNGC 2 cut(s) 86, 323
GsaI CCCAGC 1 cut(s) 351
HaeIII GGCC 2 cut(s) 117, 440
Hin1II CATG 1 cut(s) 56
HphI GGTGA 2 cut(s) 304, 439
Hpy188I TCNGA 2 cut(s) 97, 158
Hpy188III TCNNGA 1 cut(s) 277
HpyAV CCTTC 5 cut(s) 259, 400, 409, 415, 424
HpyCH4V TGCA 1 cut(s) 322
HpyF3I CTNAG 3 cut(s) 94, 243, 326
Hsp92II CATG 1 cut(s) 56
Kzo9I GATC 2 cut(s) 24, 273
LguI GCTCTTC 1 cut(s) 185
LmnI GCTCC 1 cut(s) 304
LpnPI CCDG 8 cut(s) 150, 255, 273, 296, 333, 357, 381, 387
Lsp1109I GCAGC 2 cut(s) 97, 334
MaeIII GTNAC 2 cut(s) 247, 329
MalI GATC 2 cut(s) 26, 275
MboI GATC 2 cut(s) 24, 273
MboII GAAGA 4 cut(s) 94, 199, 202, 307
MhlI GDGCHC 1 cut(s) 48
MluCI AATT 1 cut(s) 216
MslI CAYNNNNRTG 1 cut(s) 57
NcoI CCATGG 1 cut(s) 52
NdeII GATC 2 cut(s) 24, 273
NlaIII CATG 1 cut(s) 56
NmuCI GTSAC 2 cut(s) 247, 329
PciSI GCTCTTC 1 cut(s) 185
PkrI GCNGC 2 cut(s) 87, 324
Psp124BI GAGCTC 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 347
RseI CAYNNNNRTG 1 cut(s) 57
SacI GAGCTC 1 cut(s) 48
SapI GCTCTTC 1 cut(s) 185
SatI GCNGC 2 cut(s) 86, 323
Sau3AI GATC 2 cut(s) 24, 273
SduI GDGCHC 1 cut(s) 48
SetI ASST 9 cut(s) 48, 90, 197, 249, 309, 327, 387, 429, 459
SmiMI CAYNNNNRTG 1 cut(s) 57
SmlI CTYRAG 2 cut(s) 128, 179
SmoI CTYRAG 2 cut(s) 128, 179
Sse9I AATT 1 cut(s) 216
SstI GAGCTC 1 cut(s) 48
StyI CCWWGG 3 cut(s) 52, 300, 441
TasI AATT 1 cut(s) 216
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 2 cut(s) 247, 329
TseI GCWGC 2 cut(s) 85, 322
Tsp45I GTSAC 2 cut(s) 247, 329
TspDTI ATGAA 1 cut(s) 75
TspRI CASTG 1 cut(s) 334
XcmI CCANNNNNNNNNTGG 2 cut(s) 307, 448
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.