FvH4_6g15840

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0003978
Physical Location & Seq
Reverse (-)
9889470 .. 9900672
11203 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g15840.t3

Sequence Viewer

Length: 855 bp
ATGGCCAAGGACATTGAAGTAGGTGGTTTTGCTGCCAAGGACTACCACGACCCACCCCCAACACCATTGATCGATCCGGAGGAGTTTGGAAAATGGTCTTTTTACAGAGCCATCATTGCCGAGTTCATCGCTACGCTTTTGTTCTTGTACATCACTGTGCTCACTGTGATTGGATACAAGAGCCAGAGTGACACTCTCAAAGGTGGAGACCAATGTGGTGGTGTTGGCATTCTTGGCATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTTGTTTACTGCACTGCCGGAATCTCTGGAGGGCACATAAACCCTGCTGTGACATTTGGGCTGTTTTTGGCTAGGAAGGTGTCACTGCCCAGAGCTGTAATGTATATTGTGGCTCAGTCTTTGGGAGCAATATGTGGGTGTGCGCTTGTCAAATCGTTCCAGAGTGCTTTGTACACCAACTATGGTGGTGGAGCTAATGGGCTAGCTGATGGGTACAACAAAGGCACCGGTTTGGCTGCTGAGATTATTGGTACATTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGACCCCAAGAGGAATGCCAGGGACTCCCATGTCCCAGTATTGGCACCACTACCAATTGGGTTTGCTGTCTTTATGGTTCACCTTGCCACAATCCCAATCACTGGTACCGGGATCAACCCCGCAAGAAGTTTAGGAGCGGCAGTGATTTACAACAATGAGAAGGCTTGGGATGACCATTGGATCTTCTGGGTTGGACCCTTCATCGGTGCTGCCATTGCTGCCTTGTATCACCAATACATACTGAGAGCAGGAGCAGTCAAGGCTTTGGGTTCTTTCAGGAGCTCTTCCAACATATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

285

Amino Acids

30.12

Weight (kDa)

8.28

Isoelectric Point (pI)

32.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 32 - 266 1.7e-85 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 587
Acc65I GGTACC 1 cut(s) 662
AccB1I GGYRCC 3 cut(s) 494, 601, 662
AccB7I CCANNNNNTGG 1 cut(s) 659
AccBSI CCGCTC 1 cut(s) 695
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 2 cut(s) 678, 695
AclWI GGATC 3 cut(s) 68, 677, 746
AcoI YGGCCR 1 cut(s) 3
AfaI GTAC 5 cut(s) 149, 443, 485, 523, 664
AfiI CCNNNNNNNGG 3 cut(s) 598, 659, 761
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 1 cut(s) 575
AluBI AGCT 4 cut(s) 365, 464, 476, 840
AluI AGCT 4 cut(s) 365, 464, 476, 840
Alw21I GWGCWC 2 cut(s) 162, 842
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 3 cut(s) 68, 677, 746
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 3, 246
ApeKI GCWGC 4 cut(s) 32, 506, 767, 776
AsiGI ACCGGT 1 cut(s) 497
Asp718I GGTACC 1 cut(s) 662
AspLEI GCGC 1 cut(s) 415
AspS9I GGNCC 2 cut(s) 246, 752
AsuC2I CCSGG 1 cut(s) 667
AsuHPI GGTGA 2 cut(s) 629, 779
AsuNHI GCTAGC 1 cut(s) 472
AvaII GGWCC 1 cut(s) 752
BaeGI GKGCMC 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BalI TGGCCA 1 cut(s) 5
BanI GGYRCC 3 cut(s) 494, 601, 662
BanII GRGCYC 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 162, 842
BbvI GCAGC 4 cut(s) 19, 493, 754, 763
BccI CCATC 2 cut(s) 119, 473
BciT130I CCWGG 1 cut(s) 577
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 667
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 342, 473
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 5 cut(s) 33, 507, 696, 768, 777
BlsI GCNGC 5 cut(s) 34, 508, 697, 769, 778
Bme1390I CCNGG 2 cut(s) 577, 667
Bme18I GGWCC 1 cut(s) 752
BmgT120I GGNCC 2 cut(s) 246, 752
BmiI GGNNCC 4 cut(s) 496, 603, 664, 754
BmrFI CCNGG 2 cut(s) 577, 667
BmrI ACTGGG 1 cut(s) 587
BmtI GCTAGC 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 318
BpuMI CCSGG 1 cut(s) 667
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 3 cut(s) 6, 36, 576
BsaWI WCCGGW 2 cut(s) 76, 497
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 3 cut(s) 598, 659, 761
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 2 cut(s) 593, 664
Bse3DI GCAATG 3 cut(s) 114, 237, 771
BseAI TCCGGA 1 cut(s) 76
BseBI CCWGG 1 cut(s) 577
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 3 cut(s) 6, 36, 576
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 3 cut(s) 598, 659, 761
BseMI GCAATG 3 cut(s) 114, 237, 771
BseMII CTCAG 3 cut(s) 398, 501, 791
BseNI ACTGG 2 cut(s) 593, 664
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 4 cut(s) 19, 493, 754, 763
BsgI GTGCAG 1 cut(s) 265
BshFI GGCC 2 cut(s) 5, 248
BshNI GGYRCC 3 cut(s) 494, 601, 662
BshTI ACCGGT 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 162, 842
BsiSI CCGG 4 cut(s) 77, 288, 498, 666
BslFI GGGAC 2 cut(s) 575, 593
BslI CCNNNNNNNGG 3 cut(s) 598, 659, 761
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 2 cut(s) 575, 593
BsmI GAATGC 2 cut(s) 228, 577
BsnI GGCC 2 cut(s) 5, 248
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 3 cut(s) 162, 306, 842
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 2 cut(s) 147, 441
Bsp143I GATC 5 cut(s) 69, 73, 261, 669, 738
BspACI CCGC 2 cut(s) 678, 695
BspANI GGCC 2 cut(s) 5, 248
BspCNI CTCAG 3 cut(s) 397, 502, 792
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 496, 603, 664, 754
BspOI GCTAGC 1 cut(s) 476
BspPI GGATC 3 cut(s) 68, 677, 746
BspQI GCTCTTC 1 cut(s) 847
BspT107I GGYRCC 3 cut(s) 494, 601, 662
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 695
BsrDI GCAATG 3 cut(s) 114, 237, 771
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 2 cut(s) 147, 441
BsrI ACTGG 2 cut(s) 593, 664
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 3 cut(s) 6, 36, 576
BssMI GATC 5 cut(s) 69, 73, 261, 669, 738
BssT1I CCWWGG 2 cut(s) 6, 36
Bst2UI CCWGG 1 cut(s) 577
Bst4CI ACNGT 3 cut(s) 157, 166, 544
Bst6I CTCTTC 1 cut(s) 847
BstAUI TGTACA 2 cut(s) 147, 441
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 3 cut(s) 384, 510, 800
BstF5I GGATG 1 cut(s) 733
BstHHI GCGC 1 cut(s) 415
BstKTI GATC 5 cut(s) 72, 76, 264, 672, 741
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 69, 73, 261, 669, 738
BstMWI GCNNNNNNNGC 5 cut(s) 116, 234, 773, 776, 818
BstNI CCWGG 1 cut(s) 577
BstSCI CCNGG 2 cut(s) 575, 665
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 4 cut(s) 19, 493, 754, 763
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 738
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 2 cut(s) 5, 248
BsuTUI ATCGAT 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 3 cut(s) 282, 353, 705
BtsIMutI CAGTG 8 cut(s) 153, 162, 282, 353, 540, 555, 657, 705
Cac8I GCNNGC 1 cut(s) 474
CfoI GCGC 1 cut(s) 415
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 246, 752
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 5 cut(s) 148, 442, 484, 522, 663
CspAI ACCGGT 1 cut(s) 497
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 2 cut(s) 259, 587
CviQI GTAC 5 cut(s) 148, 442, 484, 522, 663
DdeI CTNAG 3 cut(s) 384, 510, 800
DpnI GATC 5 cut(s) 71, 75, 263, 671, 740
DpnII GATC 5 cut(s) 69, 73, 261, 669, 738
DrdI GACNNNNNNGTC 1 cut(s) 587
DseDI GACNNNNNNGTC 1 cut(s) 587
EaeI YGGCCR 1 cut(s) 3
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 2 cut(s) 6, 36
Eco24I GRGCYC 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 752
Eco53kI GAGCTC 1 cut(s) 840
EcoICRI GAGCTC 1 cut(s) 840
EcoRII CCWGG 1 cut(s) 575
EcoT14I CCWWGG 2 cut(s) 6, 36
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 2 cut(s) 6, 36
FaeI CATG 2 cut(s) 262, 590
FaqI GGGAC 2 cut(s) 575, 593
FatI CATG 2 cut(s) 258, 586
FauI CCCGC 1 cut(s) 685
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 5 cut(s) 33, 507, 696, 768, 777
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 5 cut(s) 33, 507, 696, 768, 777
FspBI CTAG 2 cut(s) 342, 473
GlaI GCGC 1 cut(s) 414
GluI GCNGC 5 cut(s) 33, 507, 696, 768, 777
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 2 cut(s) 5, 248
HapII CCGG 4 cut(s) 77, 288, 498, 666
HhaI GCGC 1 cut(s) 415
Hin1II CATG 2 cut(s) 262, 590
Hin6I GCGC 1 cut(s) 413
HinP1I GCGC 1 cut(s) 413
HinfI GANTC 2 cut(s) 291, 581
HpaII CCGG 4 cut(s) 77, 288, 498, 666
HphI GGTGA 2 cut(s) 629, 779
Hpy166II GTNNAC 4 cut(s) 277, 444, 538, 637
Hpy188III TCNNGA 4 cut(s) 77, 297, 430, 835
Hpy8I GTNNAC 4 cut(s) 277, 444, 538, 637
HpyAV CCTTC 3 cut(s) 340, 712, 766
HpyCH4III ACNGT 3 cut(s) 157, 166, 544
HpyCH4V TGCA 1 cut(s) 282
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 234, 773, 776, 818
HpyF3I CTNAG 3 cut(s) 384, 510, 800
Hsp92II CATG 2 cut(s) 262, 590
HspAI GCGC 1 cut(s) 413
Kpn2I TCCGGA 1 cut(s) 76
KpnI GGTACC 1 cut(s) 666
Kzo9I GATC 5 cut(s) 69, 73, 261, 669, 738
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 5 cut(s) 395, 461, 692, 809, 837
Lsp1109I GCAGC 4 cut(s) 19, 493, 754, 763
MaeI CTAG 2 cut(s) 342, 473
MaeIII GTNAC 3 cut(s) 188, 319, 351
MalI GATC 5 cut(s) 71, 75, 263, 671, 740
MbiI CCGCTC 1 cut(s) 695
MboI GATC 5 cut(s) 69, 73, 261, 669, 738
MboII GAAGA 3 cut(s) 538, 733, 834
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 3 cut(s) 162, 306, 842
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 1 cut(s) 612
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 1 cut(s) 575
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 3 cut(s) 73, 293, 561
Mox20I TGGCCA 1 cut(s) 5
MroI TCCGGA 1 cut(s) 76
MscI TGGCCA 1 cut(s) 5
MslI CAYNNNNRTG 1 cut(s) 155
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 4 cut(s) 77, 288, 498, 666
MspR9I CCNGG 2 cut(s) 577, 667
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 2 cut(s) 228, 577
MvaI CCWGG 1 cut(s) 577
MwoI GCNNNNNNNGC 5 cut(s) 116, 234, 773, 776, 818
NciI CCSGG 1 cut(s) 667
NdeII GATC 5 cut(s) 69, 73, 261, 669, 738
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 2 cut(s) 262, 590
NlaIV GGNNCC 4 cut(s) 496, 603, 664, 754
NmeAIII GCCGAG 1 cut(s) 145
NmuCI GTSAC 3 cut(s) 188, 319, 351
PciSI GCTCTTC 1 cut(s) 847
PctI GAATGC 2 cut(s) 228, 577
PfeI GAWTC 1 cut(s) 291
PflMI CCANNNNNTGG 1 cut(s) 659
PinAI ACCGGT 1 cut(s) 497
PkrI GCNGC 5 cut(s) 34, 508, 697, 769, 778
PleI GAGTC 1 cut(s) 575
PpsI GAGTC 1 cut(s) 575
Psp124BI GAGCTC 1 cut(s) 842
Psp6I CCWGG 1 cut(s) 575
PspGI CCWGG 1 cut(s) 575
PspN4I GGNNCC 4 cut(s) 496, 603, 664, 754
PspPI GGNCC 2 cut(s) 246, 752
PsuI RGATCY 1 cut(s) 738
RsaI GTAC 5 cut(s) 149, 443, 485, 523, 664
RsaNI GTAC 5 cut(s) 148, 442, 484, 522, 663
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 842
SapI GCTCTTC 1 cut(s) 847
SatI GCNGC 5 cut(s) 33, 507, 696, 768, 777
Sau3AI GATC 5 cut(s) 69, 73, 261, 669, 738
Sau96I GGNCC 2 cut(s) 246, 752
SchI GAGTC 1 cut(s) 575
ScrFI CCNGG 2 cut(s) 577, 667
SduI GDGCHC 3 cut(s) 162, 306, 842
SetI ASST 8 cut(s) 25, 205, 351, 367, 466, 478, 642, 842
SinI GGWCC 1 cut(s) 752
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 1 cut(s) 612
SsiI CCGC 2 cut(s) 678, 695
SspMI CTAG 2 cut(s) 342, 473
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 2 cut(s) 575, 665
StyI CCWWGG 2 cut(s) 6, 36
TaaI ACNGT 3 cut(s) 157, 166, 544
TaqI TCGA 1 cut(s) 72
TasI AATT 1 cut(s) 612
TatI WGTACW 2 cut(s) 147, 441
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 1 cut(s) 291
TscAI CASTG 8 cut(s) 160, 169, 289, 360, 547, 562, 664, 705
TseFI GTSAC 3 cut(s) 188, 319, 351
TseI GCWGC 4 cut(s) 32, 506, 767, 776
Tsp45I GTSAC 3 cut(s) 188, 319, 351
TspDTI ATGAA 2 cut(s) 115, 748
TspRI CASTG 8 cut(s) 160, 169, 289, 360, 547, 562, 664, 705
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 752
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 2 cut(s) 342, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.