RLG00000024341

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr5
OG0003978
Physical Location & Seq
Forward (+)
34913232 .. 34914584
1353 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000024341

Sequence Viewer

Length: 855 bp
ATGGGCAAGGACATTGAAGTTGGAGGGTTTGCTGCCAAGGACTACCATGACCCACCGCCAACACCATTGATCGATCCGGAGGAGTTCGGAAAATGGTCCTTTTACAGAGCCATCATTGCAGAGTTCATCGCTACGCTTTTGTTCTTGTACATCACTGTGCTCACTGTGATTGGATACAAGAGCCAGAGTGACACTCTCAAAGGTGGAGAGCAATGTGGTGGTGTTGGGATTCTTGGCATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTAGTTTACTGCACTGCTGGAATCTCTGGAGGGCACATAAACCCAGCTGTGACATTTGCGCTGTTTTTGGCTAGGAAGGTGTCACTGCCCAGAGCTATATTGTATATTTCGGCTCAGTCCTTGGGAGCAATATGTGGGTGTGGGCTTGTCAAATTGTTCCAGAGTGCTTTATACACAAACTATGGTGGTGGAGCTAATGGGTTAGCTGATGGGTACAGCAAAGGCACCGGTTTGGCAGCTGAGATTATCGGTACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGATCCCAAGAGAAATGCAAGGGATTCCCATGTCCCAGTATTGGCACCACTTCCAATTGGGTTTGCTGTGTTCATGGTTCACCTTGCCACAATCCCAATCACTGGCACAGGGATCAACCCCGCTCGAAGTTTAGGGGCGGCAGTGATTTACAACAATGAGAAGGCTTGGGATGACCATTGGATCTTCTGGGTTGGACCCTTCATTGGTGCAGCTATTGCAGCCTTGTATCACCAGTACATACTGAGAGCAGGAGCAGCTAAGGCTTTGGGTTCTTTCAGGAGCTCCTCCAACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

285

Amino Acids

30.08

Weight (kDa)

8.28

Isoelectric Point (pI)

31.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 29 - 263 1.3e-84 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 3 cut(s) 494, 521, 601
AccB7I CCANNNNNTGG 1 cut(s) 659
AccBSI CCGCTC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 3 cut(s) 56, 678, 695
AclWI GGATC 4 cut(s) 68, 554, 677, 746
AfaI GTAC 4 cut(s) 149, 485, 523, 794
AfiI CCNNNNNNNGG 3 cut(s) 598, 659, 761
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 1 cut(s) 17
AluBI AGCT 8 cut(s) 317, 365, 464, 476, 509, 770, 815, 840
AluI AGCT 8 cut(s) 317, 365, 464, 476, 509, 770, 815, 840
Alw21I GWGCWC 2 cut(s) 162, 842
AlwI GGATC 4 cut(s) 68, 554, 677, 746
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 5 cut(s) 32, 506, 767, 776, 812
AsiGI ACCGGT 1 cut(s) 497
Asp718I GGTACC 1 cut(s) 521
AspLEI GCGC 1 cut(s) 331
AspS9I GGNCC 3 cut(s) 96, 246, 752
AsuHPI GGTGA 2 cut(s) 629, 779
AvaII GGWCC 2 cut(s) 96, 752
BaeGI GKGCMC 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 3 cut(s) 494, 521, 601
BanII GRGCYC 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 162, 842
BbvI GCAGC 5 cut(s) 19, 518, 779, 788, 824
BccI CCATC 2 cut(s) 119, 473
BciVI GTATCC 1 cut(s) 167
BfaI CTAG 2 cut(s) 272, 342
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 6 cut(s) 33, 507, 696, 768, 777, 813
BlsI GCNGC 6 cut(s) 34, 508, 697, 769, 778, 814
Bme18I GGWCC 2 cut(s) 96, 752
BmgT120I GGNCC 3 cut(s) 96, 246, 752
BmiI GGNNCC 4 cut(s) 496, 523, 603, 754
BmrI ACTGGG 1 cut(s) 587
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 816
Bsa29I ATCGAT 1 cut(s) 72
BsaJI CCNNGG 2 cut(s) 36, 390
BsaWI WCCGGW 2 cut(s) 76, 497
Bsc4I CCNNNNNNNGG 3 cut(s) 598, 659, 761
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 3 cut(s) 593, 664, 790
Bse3DI GCAATG 3 cut(s) 114, 218, 237
BseAI TCCGGA 1 cut(s) 76
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 36, 390
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 3 cut(s) 598, 659, 761
BseMI GCAATG 3 cut(s) 114, 218, 237
BseMII CTCAG 3 cut(s) 398, 501, 791
BseNI ACTGG 3 cut(s) 593, 664, 790
BseRI GAGGAG 2 cut(s) 95, 832
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 5 cut(s) 19, 518, 779, 788, 824
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 2 cut(s) 265, 786
BshFI GGCC 1 cut(s) 248
BshNI GGYRCC 3 cut(s) 494, 521, 601
BshTI ACCGGT 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 162, 842
BsiSI CCGG 2 cut(s) 77, 498
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 3 cut(s) 598, 659, 761
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 1 cut(s) 248
Bsp1286I GDGCHC 3 cut(s) 162, 306, 842
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BspACI CCGC 3 cut(s) 56, 678, 695
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 3 cut(s) 397, 502, 792
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 4 cut(s) 496, 523, 603, 754
BspPI GGATC 4 cut(s) 68, 554, 677, 746
BspT107I GGYRCC 3 cut(s) 494, 521, 601
BsrBI CCGCTC 1 cut(s) 680
BsrDI GCAATG 3 cut(s) 114, 218, 237
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 3 cut(s) 593, 664, 790
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 36, 390
BssMI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BssT1I CCWWGG 2 cut(s) 36, 390
Bst4CI ACNGT 3 cut(s) 157, 166, 544
BstAPI GCANNNNNTGC 1 cut(s) 773
BstAUI TGTACA 1 cut(s) 147
BstDEI CTNAG 4 cut(s) 384, 510, 800, 816
BstF5I GGATG 1 cut(s) 733
BstHHI GCGC 1 cut(s) 331
BstKTI GATC 6 cut(s) 72, 76, 264, 562, 672, 741
BstMBI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BstMWI GCNNNNNNNGC 5 cut(s) 116, 773, 776, 812, 818
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 5 cut(s) 19, 518, 779, 788, 824
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 738
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 248
BsuTUI ATCGAT 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 3 cut(s) 282, 353, 705
BtsIMutI CAGTG 7 cut(s) 153, 162, 282, 353, 555, 657, 705
CfoI GCGC 1 cut(s) 331
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 3 cut(s) 96, 246, 752
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 4 cut(s) 148, 484, 522, 793
CspAI ACCGGT 1 cut(s) 497
CviAII CATG 4 cut(s) 47, 259, 587, 631
CviQI GTAC 4 cut(s) 148, 484, 522, 793
DdeI CTNAG 4 cut(s) 384, 510, 800, 816
DpnI GATC 6 cut(s) 71, 75, 263, 561, 671, 740
DpnII GATC 6 cut(s) 69, 73, 261, 559, 669, 738
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 2 cut(s) 36, 390
Eco24I GRGCYC 1 cut(s) 842
Eco47I GGWCC 2 cut(s) 96, 752
Eco53kI GAGCTC 1 cut(s) 840
EcoICRI GAGCTC 1 cut(s) 840
EcoT14I CCWWGG 2 cut(s) 36, 390
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 2 cut(s) 36, 390
FaeI CATG 4 cut(s) 50, 262, 590, 634
FaqI GGGAC 1 cut(s) 575
FatI CATG 4 cut(s) 46, 258, 586, 630
FauI CCCGC 1 cut(s) 685
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 6 cut(s) 33, 507, 696, 768, 777, 813
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 6 cut(s) 33, 507, 696, 768, 777, 813
FspBI CTAG 2 cut(s) 272, 342
GlaI GCGC 1 cut(s) 330
GluI GCNGC 6 cut(s) 33, 507, 696, 768, 777, 813
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 1 cut(s) 248
HapII CCGG 2 cut(s) 77, 498
HhaI GCGC 1 cut(s) 331
Hin1II CATG 4 cut(s) 50, 262, 590, 634
Hin6I GCGC 1 cut(s) 329
HinP1I GCGC 1 cut(s) 329
HinfI GANTC 3 cut(s) 229, 291, 581
HpaII CCGG 2 cut(s) 77, 498
HphI GGTGA 2 cut(s) 629, 779
Hpy166II GTNNAC 3 cut(s) 277, 538, 637
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 4 cut(s) 77, 297, 430, 835
Hpy8I GTNNAC 3 cut(s) 277, 538, 637
HpyAV CCTTC 3 cut(s) 340, 712, 766
HpyCH4III ACNGT 3 cut(s) 157, 166, 544
HpyCH4V TGCA 5 cut(s) 119, 282, 575, 767, 776
HpyF10VI GCNNNNNNNGC 5 cut(s) 116, 773, 776, 812, 818
HpyF3I CTNAG 4 cut(s) 384, 510, 800, 816
Hsp92II CATG 4 cut(s) 50, 262, 590, 634
HspAI GCGC 1 cut(s) 329
Kpn2I TCCGGA 1 cut(s) 76
KpnI GGTACC 1 cut(s) 525
Kzo9I GATC 6 cut(s) 69, 73, 261, 559, 669, 738
LmnI GCTCC 5 cut(s) 395, 461, 809, 837, 845
Lsp1109I GCAGC 5 cut(s) 19, 518, 779, 788, 824
MaeI CTAG 2 cut(s) 272, 342
MaeIII GTNAC 3 cut(s) 188, 319, 351
MalI GATC 6 cut(s) 71, 75, 263, 561, 671, 740
MbiI CCGCTC 1 cut(s) 680
MboI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
MboII GAAGA 2 cut(s) 538, 733
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 3 cut(s) 162, 306, 842
MluCI AATT 2 cut(s) 422, 612
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 4 cut(s) 17, 73, 293, 853
MroI TCCGGA 1 cut(s) 76
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 2 cut(s) 317, 509
MspI CCGG 2 cut(s) 77, 498
MunI CAATTG 1 cut(s) 612
MwoI GCNNNNNNNGC 5 cut(s) 116, 773, 776, 812, 818
NdeII GATC 6 cut(s) 69, 73, 261, 559, 669, 738
NlaIII CATG 4 cut(s) 50, 262, 590, 634
NlaIV GGNNCC 4 cut(s) 496, 523, 603, 754
NmuCI GTSAC 3 cut(s) 188, 319, 351
PfeI GAWTC 3 cut(s) 229, 291, 581
PflMI CCANNNNNTGG 1 cut(s) 659
PinAI ACCGGT 1 cut(s) 497
PkrI GCNGC 6 cut(s) 34, 508, 697, 769, 778, 814
Psp124BI GAGCTC 1 cut(s) 842
PspFI CCCAGC 1 cut(s) 313
PspN4I GGNNCC 4 cut(s) 496, 523, 603, 754
PspPI GGNCC 3 cut(s) 96, 246, 752
PsuI RGATCY 1 cut(s) 738
PvuII CAGCTG 2 cut(s) 317, 509
RsaI GTAC 4 cut(s) 149, 485, 523, 794
RsaNI GTAC 4 cut(s) 148, 484, 522, 793
RseI CAYNNNNRTG 1 cut(s) 155
SacI GAGCTC 1 cut(s) 842
SatI GCNGC 6 cut(s) 33, 507, 696, 768, 777, 813
Sau3AI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
Sau96I GGNCC 3 cut(s) 96, 246, 752
SduI GDGCHC 3 cut(s) 162, 306, 842
SinI GGWCC 2 cut(s) 96, 752
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 2 cut(s) 422, 612
SsiI CCGC 3 cut(s) 56, 678, 695
SspMI CTAG 2 cut(s) 272, 342
SstI GAGCTC 1 cut(s) 842
StyI CCWWGG 2 cut(s) 36, 390
TaaI ACNGT 3 cut(s) 157, 166, 544
TaqI TCGA 2 cut(s) 72, 682
TasI AATT 2 cut(s) 422, 612
TatI WGTACW 2 cut(s) 147, 792
TauI GCSGC 1 cut(s) 698
TfiI GAWTC 3 cut(s) 229, 291, 581
TscAI CASTG 7 cut(s) 160, 169, 289, 360, 562, 664, 705
TseFI GTSAC 3 cut(s) 188, 319, 351
TseI GCWGC 5 cut(s) 32, 506, 767, 776, 812
Tsp45I GTSAC 3 cut(s) 188, 319, 351
TspDTI ATGAA 3 cut(s) 115, 619, 748
TspRI CASTG 7 cut(s) 160, 169, 289, 360, 562, 664, 705
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 2 cut(s) 96, 752
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 2 cut(s) 272, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.