MD04G1098300.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr04
OG0003978
Physical Location & Seq
Forward (+)
18342646 .. 18344689
2044 bp
Loading structure...
UTR
Exon/CDS
Intron
MD04G1098300.v1.1.491

Sequence Viewer

Length: 717 bp
ATGCCATGGTGCTTAACCCAACATCGATGTTCCATTGCTAAAGACATTGAGGTTGGAAGTGGGAACCCAGCCAAGGACTACCAGGATCCTCCGCCGGCGCCGCTGATTGACCCAGAGGAGCTAGGGAAGTGGTCATTCTACAGAGCCATCATAGCTGAGTTTATTGCCACCCTCTTGTTTTTGTACGTCACTGTGCTCACTGTGATTGGTTACAAGCAACAGACTGACACTACCCTAAATGGAAACCAATGTGGTGGAGTTGGCATTCATGGTATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTTGTTTATTGCACTGCTGGCATCTCTGGTGGGGGGCATATAAACCCTGCAGTCACATTTGGGCTGTTTTTGACAAGGAAGGTGTCACTGATGAGAGCCATATTGTACATGGTGGCTCAGTCGTTGGGAGCCATATGTGGTGTTGGGCTTGTTAAAGCTTTCCAAAGTGCTTACTATACCAAGTATGGTGGTGGAGCCAATAAGCTTGCCGATGGCTACAGCACAGGCACTGGGTTGGGTGCTGAGATTGTTGGCACCTTTGTTCTTGTCTACACTGTCTTGTCTGCCACTGATCCGGAGCTAAAAGCAAGAAATTCCTATGTCCCAGTATTGGCACCACTGCCAATTGGGTTTGCTGTGTTTATTGTGCACCTTGCCACAATCCCAATCACTGGCACTGGTATCAACTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

239

Amino Acids

25.2

Weight (kDa)

7.62

Isoelectric Point (pI)

28.76

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 41 - 238 3.9e-60 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 3 cut(s) 97, 560, 640
AccB7I CCANNNNNTGG 1 cut(s) 698
AccI GTMKAC 1 cut(s) 576
AccIII TCCGGA 1 cut(s) 601
AciI CCGC 2 cut(s) 92, 101
AclWI GGATC 3 cut(s) 80, 93, 593
AcsI RAATTY 1 cut(s) 619
AcyI GRCGYC 1 cut(s) 98
AfaI GTAC 2 cut(s) 185, 413
AfiI CCNNNNNNNGG 3 cut(s) 73, 637, 698
AjnI CCWGG 1 cut(s) 81
AluBI AGCT 5 cut(s) 121, 155, 464, 511, 607
AluI AGCT 5 cut(s) 121, 155, 464, 511, 607
Alw21I GWGCWC 2 cut(s) 198, 678
Alw44I GTGCAC 1 cut(s) 674
AlwI GGATC 3 cut(s) 80, 93, 593
AlwNI CAGNNNCTG 1 cut(s) 536
Aor13HI TCCGGA 1 cut(s) 601
AoxI GGCC 1 cut(s) 282
ApaLI GTGCAC 1 cut(s) 674
ApoI RAATTY 1 cut(s) 619
AspLEI GCGC 1 cut(s) 100
AspS9I GGNCC 1 cut(s) 282
BaeGI GKGCMC 1 cut(s) 678
BamHI GGATCC 1 cut(s) 85
BanI GGYRCC 3 cut(s) 97, 560, 640
Bbv12I GWGCWC 2 cut(s) 198, 678
BccI CCATC 2 cut(s) 155, 512
BciT130I CCWGG 1 cut(s) 83
BfaI CTAG 2 cut(s) 122, 715
BfmI CTRYAG 3 cut(s) 139, 354, 523
BfoI RGCGCY 1 cut(s) 101
BisI GCNGC 1 cut(s) 101
BlsI GCNGC 1 cut(s) 102
Bme1390I CCNGG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 282
BmiI GGNNCC 7 cut(s) 65, 87, 99, 436, 502, 562, 642
BmrFI CCNGG 1 cut(s) 83
BmrI ACTGGG 2 cut(s) 546, 626
BmsI GCATC 1 cut(s) 336
BmuI ACTGGG 2 cut(s) 546, 626
Bsa29I ATCGAT 1 cut(s) 25
BsaHI GRCGYC 1 cut(s) 98
BsaJI CCNNGG 2 cut(s) 5, 72
BsaWI WCCGGW 1 cut(s) 601
Bsc4I CCNNNNNNNGG 3 cut(s) 73, 637, 698
Bse118I RCCGGY 1 cut(s) 94
Bse1I ACTGG 4 cut(s) 541, 632, 703, 709
Bse3DI GCAATG 1 cut(s) 33
BseAI TCCGGA 1 cut(s) 601
BseBI CCWGG 1 cut(s) 83
BseCI ATCGAT 1 cut(s) 25
BseDI CCNNGG 2 cut(s) 5, 72
BseLI CCNNNNNNNGG 3 cut(s) 73, 637, 698
BseMI GCAATG 1 cut(s) 33
BseMII CTCAG 3 cut(s) 147, 437, 540
BseNI ACTGG 4 cut(s) 541, 632, 703, 709
BseRI GAGGAG 1 cut(s) 131
BseSI GKGCMC 1 cut(s) 678
BseYI CCCAGC 1 cut(s) 67
BshFI GGCC 1 cut(s) 284
BshNI GGYRCC 3 cut(s) 97, 560, 640
BshVI ATCGAT 1 cut(s) 25
BsiHKAI GWGCWC 2 cut(s) 198, 678
BsiSI CCGG 2 cut(s) 95, 602
BslFI GGGAC 1 cut(s) 614
BslI CCNNNNNNNGG 3 cut(s) 73, 637, 698
BsmFI GGGAC 1 cut(s) 614
BsmI GAATGC 1 cut(s) 264
BsnI GGCC 1 cut(s) 284
Bsp1286I GDGCHC 2 cut(s) 198, 678
Bsp13I TCCGGA 1 cut(s) 601
Bsp1407I TGTACA 1 cut(s) 411
Bsp143I GATC 3 cut(s) 85, 297, 598
Bsp19I CCATGG 1 cut(s) 5
BspACI CCGC 2 cut(s) 92, 101
BspANI GGCC 1 cut(s) 284
BspCNI CTCAG 3 cut(s) 148, 436, 541
BspDI ATCGAT 1 cut(s) 25
BspEI TCCGGA 1 cut(s) 601
BspLI GGNNCC 7 cut(s) 65, 87, 99, 436, 502, 562, 642
BspMAI CTGCAG 1 cut(s) 358
BspPI GGATC 3 cut(s) 80, 93, 593
BspT107I GGYRCC 3 cut(s) 97, 560, 640
BsrDI GCAATG 1 cut(s) 33
BsrFI RCCGGY 1 cut(s) 94
BsrGI TGTACA 1 cut(s) 411
BsrI ACTGG 4 cut(s) 541, 632, 703, 709
BssAI RCCGGY 1 cut(s) 94
BssECI CCNNGG 2 cut(s) 5, 72
BssMI GATC 3 cut(s) 85, 297, 598
BssNI GRCGYC 1 cut(s) 98
BssT1I CCWWGG 2 cut(s) 5, 72
Bst2UI CCWGG 1 cut(s) 83
Bst4CI ACNGT 3 cut(s) 193, 202, 583
BstACI GRCGYC 1 cut(s) 98
BstAUI TGTACA 1 cut(s) 411
BstC8I GCNNGC 3 cut(s) 96, 325, 513
BstDEI CTNAG 3 cut(s) 156, 423, 549
BstDSI CCRYGG 1 cut(s) 5
BstH2I RGCGCY 1 cut(s) 101
BstHHI GCGC 1 cut(s) 100
BstKTI GATC 3 cut(s) 88, 300, 601
BstMBI GATC 3 cut(s) 85, 297, 598
BstMWI GCNNNNNNNGC 3 cut(s) 100, 152, 324
BstNI CCWGG 1 cut(s) 83
BstSCI CCNGG 1 cut(s) 81
BstSFI CTRYAG 3 cut(s) 139, 354, 523
BstSLI GKGCMC 1 cut(s) 678
BstX2I RGATCY 1 cut(s) 85
BstXI CCANNNNNNTGG 1 cut(s) 254
BstYI RGATCY 1 cut(s) 85
Bsu15I ATCGAT 1 cut(s) 25
BsuRI GGCC 1 cut(s) 284
BsuTUI ATCGAT 1 cut(s) 25
BtgI CCRYGG 1 cut(s) 5
BtsI GCAGTG 2 cut(s) 318, 644
Cac8I GCNNGC 3 cut(s) 96, 325, 513
CaiI CAGNNNCTG 1 cut(s) 536
CfoI GCGC 1 cut(s) 100
Cfr10I RCCGGY 1 cut(s) 94
Cfr13I GGNCC 1 cut(s) 282
ClaI ATCGAT 1 cut(s) 25
Csp6I GTAC 2 cut(s) 184, 412
CspCI CAANNNNNGTGG 2 cut(s) 475, 510
CviAII CATG 4 cut(s) 6, 269, 295, 415
CviQI GTAC 2 cut(s) 184, 412
DdeI CTNAG 3 cut(s) 156, 423, 549
DinI GGCGCC 1 cut(s) 99
DpnI GATC 3 cut(s) 87, 299, 600
DpnII GATC 3 cut(s) 85, 297, 598
EciI GGCGGA 1 cut(s) 81
Eco130I CCWWGG 2 cut(s) 5, 72
EcoRII CCWGG 1 cut(s) 81
EcoT14I CCWWGG 2 cut(s) 5, 72
EgeI GGCGCC 1 cut(s) 99
EheI GGCGCC 1 cut(s) 99
ErhI CCWWGG 2 cut(s) 5, 72
FaeI CATG 4 cut(s) 9, 272, 298, 418
FaqI GGGAC 1 cut(s) 614
FatI CATG 4 cut(s) 5, 268, 294, 414
FauNDI CATATG 1 cut(s) 440
FblI GTMKAC 1 cut(s) 576
Fnu4HI GCNGC 1 cut(s) 101
Fsp4HI GCNGC 1 cut(s) 101
FspBI CTAG 2 cut(s) 122, 715
GlaI GCGC 1 cut(s) 99
GluI GCNGC 1 cut(s) 101
GsaI CCCAGC 1 cut(s) 71
HaeII RGCGCY 1 cut(s) 101
HaeIII GGCC 1 cut(s) 284
HapII CCGG 2 cut(s) 95, 602
HhaI GCGC 1 cut(s) 100
Hin1I GRCGYC 1 cut(s) 98
Hin1II CATG 4 cut(s) 9, 272, 298, 418
Hin6I GCGC 1 cut(s) 98
HinP1I GCGC 1 cut(s) 98
HindIII AAGCTT 2 cut(s) 462, 509
HpaII CCGG 2 cut(s) 95, 602
Hpy166II GTNNAC 2 cut(s) 577, 676
Hpy188III TCNNGA 1 cut(s) 602
Hpy8I GTNNAC 2 cut(s) 577, 676
HpyAV CCTTC 1 cut(s) 379
HpyCH4III ACNGT 3 cut(s) 193, 202, 583
HpyCH4IV ACGT 1 cut(s) 186
HpyCH4V TGCA 3 cut(s) 318, 356, 676
HpyF10VI GCNNNNNNNGC 3 cut(s) 100, 152, 324
HpyF3I CTNAG 3 cut(s) 156, 423, 549
HpySE526I ACGT 1 cut(s) 186
Hsp92I GRCGYC 1 cut(s) 98
Hsp92II CATG 4 cut(s) 9, 272, 298, 418
HspAI GCGC 1 cut(s) 98
KasI GGCGCC 1 cut(s) 97
Kpn2I TCCGGA 1 cut(s) 601
KroI GCCGGC 1 cut(s) 94
KroNI GCCGGC 1 cut(s) 96
Kzo9I GATC 3 cut(s) 85, 297, 598
LmnI GCTCC 4 cut(s) 118, 434, 500, 604
LweI GCATC 1 cut(s) 336
MaeI CTAG 2 cut(s) 122, 715
MaeII ACGT 1 cut(s) 186
MaeIII GTNAC 4 cut(s) 187, 209, 358, 390
MalI GATC 3 cut(s) 87, 299, 600
MboI GATC 3 cut(s) 85, 297, 598
MfeI CAATTG 1 cut(s) 651
MflI RGATCY 1 cut(s) 85
MhlI GDGCHC 2 cut(s) 198, 678
MluCI AATT 2 cut(s) 619, 651
Mly113I GGCGCC 1 cut(s) 98
MmeI TCCRAC 1 cut(s) 34
MnlI CCTC 4 cut(s) 43, 99, 109, 182
MreI CGCCGGCG 1 cut(s) 94
MroI TCCGGA 1 cut(s) 601
MroNI GCCGGC 1 cut(s) 94
MseI TTAA 2 cut(s) 14, 459
MspA1I CMGCKG 1 cut(s) 103
MspI CCGG 2 cut(s) 95, 602
MspR9I CCNGG 1 cut(s) 83
MunI CAATTG 1 cut(s) 651
Mva1269I GAATGC 1 cut(s) 264
MvaI CCWGG 1 cut(s) 83
MwoI GCNNNNNNNGC 3 cut(s) 100, 152, 324
NaeI GCCGGC 1 cut(s) 96
NarI GGCGCC 1 cut(s) 98
NcoI CCATGG 1 cut(s) 5
NdeI CATATG 1 cut(s) 440
NdeII GATC 3 cut(s) 85, 297, 598
NgoMIV GCCGGC 1 cut(s) 94
NlaIII CATG 4 cut(s) 9, 272, 298, 418
NlaIV GGNNCC 7 cut(s) 65, 87, 99, 436, 502, 562, 642
NmuCI GTSAC 3 cut(s) 187, 358, 390
PctI GAATGC 1 cut(s) 264
PdiI GCCGGC 1 cut(s) 96
PflMI CCANNNNNTGG 1 cut(s) 698
PkrI GCNGC 1 cut(s) 102
PluTI GGCGCC 1 cut(s) 101
Psp6I CCWGG 1 cut(s) 81
PspFI CCCAGC 1 cut(s) 67
PspGI CCWGG 1 cut(s) 81
PspN4I GGNNCC 7 cut(s) 65, 87, 99, 436, 502, 562, 642
PspPI GGNCC 1 cut(s) 282
PstI CTGCAG 1 cut(s) 358
PstNI CAGNNNCTG 1 cut(s) 536
PsuI RGATCY 1 cut(s) 85
RsaI GTAC 2 cut(s) 185, 413
RsaNI GTAC 2 cut(s) 184, 412
SaqAI TTAA 2 cut(s) 14, 459
SatI GCNGC 1 cut(s) 101
Sau3AI GATC 3 cut(s) 85, 297, 598
Sau96I GGNCC 1 cut(s) 282
ScrFI CCNGG 1 cut(s) 83
SduI GDGCHC 2 cut(s) 198, 678
SfaNI GCATC 1 cut(s) 336
SfcI CTRYAG 3 cut(s) 139, 354, 523
SfoI GGCGCC 1 cut(s) 99
SgrAI CRCCGGYG 1 cut(s) 94
Sse9I AATT 2 cut(s) 619, 651
SsiI CCGC 2 cut(s) 92, 101
SspDI GGCGCC 1 cut(s) 97
SspMI CTAG 2 cut(s) 122, 715
StyD4I CCNGG 1 cut(s) 81
StyI CCWWGG 2 cut(s) 5, 72
TaaI ACNGT 3 cut(s) 193, 202, 583
TaiI ACGT 1 cut(s) 189
TaqI TCGA 1 cut(s) 25
TasI AATT 2 cut(s) 619, 651
TatI WGTACW 1 cut(s) 411
TauI GCSGC 1 cut(s) 103
Tru1I TTAA 2 cut(s) 14, 459
Tru9I TTAA 2 cut(s) 14, 459
TseFI GTSAC 3 cut(s) 187, 358, 390
Tsp45I GTSAC 3 cut(s) 187, 358, 390
TspDTI ATGAA 1 cut(s) 257
Van91I CCANNNNNTGG 1 cut(s) 698
VneI GTGCAC 1 cut(s) 674
XapI RAATTY 1 cut(s) 619
XcmI CCANNNNNNNNNTGG 1 cut(s) 412
XmiI GTMKAC 1 cut(s) 576
XspI CTAG 2 cut(s) 122, 715
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.