Rw3G014300

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr3
OG0003978
Physical Location & Seq
Reverse (-)
14264481 .. 14265929
1449 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw3G014300.1

Sequence Viewer

Length: 855 bp
ATGGGCCGCGATATTGAAGTTGGAGGGTTTGCTGCCAAGGACTACCATGACCCACCGCCAACACCATTGATCGATCCGGAGGAGTTCGGAAAATGGTCCTTTTACAGAGCCATCATTGCAGAATTCATCGCTACACTTTTGTTCCTGTATATCACTGTGCTCACTGTGATTGGATACAAGAGCCAGAGTGACACTCTCAAAGGTGGAGACCAATGTGGTGGTGTTGGCATTCTTGGCATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTTGTTTACTGCACTGCTGGAATCTCTGGAGGGCACATAAACCCTGCTGTGACATTTGGGCTGTTTTTGGCTAGGAAGGTGTCATTACCCAGAGCTGTATTGTATATTGTGGCTCAATCCTTGGGAGCAATATGTGGGTGTGGGCTTGTGAAATCATTCCAGAGTGCTTTGTACACCAACTATGGTGGTGGAGCTAATGGGCTAGCTGATGGGTACAGCAAAGGCACTGGATTGGCTGCTGAGATTATTGGCACCTTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGATCCCAAGAGAAATGCAAGGGATTCCCATGTCCCAGTATTGGCACCACTACCAATTGGGTTTGCTGTGTTCATGGTTCACCTTGCCACAATCCCAATCACCGGCACAGGGATCAACCCGGCTCGAAGTTTCGGAGCTGCAGTGATATACAACAATGAGAAGGCTTGGGATGACCATTGGATCTTCTGGGTTGGACCCTTCATTGGTGCAGCCATTGCCGCTTTGTATCACCAAAATATATTGAGAGCAGGAGCTGCTAAGGCTTCGGGTTCTTTTAGGAGCTCTTCCAACATATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

284

Amino Acids

30.01

Weight (kDa)

8.29

Isoelectric Point (pI)

32.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 29 - 263 1.6e-85 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 521, 601
AccI GTMKAC 1 cut(s) 537
AccII CGCG 1 cut(s) 9
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 3 cut(s) 7, 56, 777
AclWI GGATC 4 cut(s) 68, 554, 677, 746
AcsI RAATTY 1 cut(s) 122
AfaI GTAC 2 cut(s) 443, 485
AfiI CCNNNNNNNGG 4 cut(s) 598, 659, 666, 761
AgsI TTSAA 1 cut(s) 17
AluBI AGCT 6 cut(s) 365, 464, 476, 695, 812, 840
AluI AGCT 6 cut(s) 365, 464, 476, 695, 812, 840
Alw21I GWGCWC 2 cut(s) 162, 842
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 4 cut(s) 68, 554, 677, 746
AlwNI CAGNNNCTG 1 cut(s) 812
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 2 cut(s) 4, 246
ApeKI GCWGC 5 cut(s) 32, 506, 695, 767, 812
ApoI RAATTY 1 cut(s) 122
Asp700I GAANNNNTTC 1 cut(s) 425
AspS9I GGNCC 4 cut(s) 4, 96, 246, 752
AsuC2I CCSGG 1 cut(s) 677
AsuHPI GGTGA 3 cut(s) 629, 649, 779
AsuNHI GCTAGC 1 cut(s) 472
AvaII GGWCC 2 cut(s) 96, 752
BaeGI GKGCMC 1 cut(s) 306
BanI GGYRCC 2 cut(s) 521, 601
BanII GRGCYC 1 cut(s) 842
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 2 cut(s) 162, 842
BbvI GCAGC 5 cut(s) 19, 493, 682, 779, 799
BccI CCATC 2 cut(s) 119, 473
BciVI GTATCC 1 cut(s) 167
BcnI CCSGG 1 cut(s) 677
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 342, 473
BfmI CTRYAG 1 cut(s) 696
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 7 cut(s) 7, 33, 507, 696, 768, 777, 813
BlsI GCNGC 7 cut(s) 8, 34, 508, 697, 769, 778, 814
Bme1390I CCNGG 1 cut(s) 677
Bme18I GGWCC 2 cut(s) 96, 752
BmgT120I GGNCC 4 cut(s) 4, 96, 246, 752
BmiI GGNNCC 3 cut(s) 523, 603, 754
BmrFI CCNGG 1 cut(s) 677
BmrI ACTGGG 1 cut(s) 587
BmtI GCTAGC 1 cut(s) 476
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 318
Bpu10I CCTNAGC 1 cut(s) 816
BpuMI CCSGG 1 cut(s) 677
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 36, 390
BsaWI WCCGGW 1 cut(s) 76
BsaXI ACNNNNNCTCC 2 cut(s) 684, 714
Bsc4I CCNNNNNNNGG 4 cut(s) 598, 659, 666, 761
Bse118I RCCGGY 1 cut(s) 659
Bse1I ACTGG 2 cut(s) 502, 593
Bse3DI GCAATG 3 cut(s) 114, 237, 771
BseAI TCCGGA 1 cut(s) 76
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 36, 390
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 4 cut(s) 598, 659, 666, 761
BseMI GCAATG 3 cut(s) 114, 237, 771
BseMII CTCAG 1 cut(s) 501
BseNI ACTGG 2 cut(s) 502, 593
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 5 cut(s) 19, 493, 682, 779, 799
BsgI GTGCAG 2 cut(s) 265, 786
Bsh1236I CGCG 1 cut(s) 9
BshFI GGCC 2 cut(s) 6, 248
BshNI GGYRCC 2 cut(s) 521, 601
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 2 cut(s) 162, 842
BsiSI CCGG 3 cut(s) 77, 660, 677
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 4 cut(s) 598, 659, 666, 761
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 575
BsmI GAATGC 1 cut(s) 228
BsnI GGCC 2 cut(s) 6, 248
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 3 cut(s) 162, 306, 842
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 441
Bsp143I GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BspACI CCGC 3 cut(s) 7, 56, 777
BspANI GGCC 2 cut(s) 6, 248
BspCNI CTCAG 1 cut(s) 502
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 76
BspFNI CGCG 1 cut(s) 9
BspLI GGNNCC 3 cut(s) 523, 603, 754
BspMAI CTGCAG 1 cut(s) 700
BspOI GCTAGC 1 cut(s) 476
BspPI GGATC 4 cut(s) 68, 554, 677, 746
BspQI GCTCTTC 1 cut(s) 847
BspT107I GGYRCC 2 cut(s) 521, 601
BspTNI GGTCTC 1 cut(s) 201
BsrDI GCAATG 3 cut(s) 114, 237, 771
BsrFI RCCGGY 1 cut(s) 659
BsrGI TGTACA 1 cut(s) 441
BsrI ACTGG 2 cut(s) 502, 593
BssAI RCCGGY 1 cut(s) 659
BssECI CCNNGG 2 cut(s) 36, 390
BssMI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BssT1I CCWWGG 2 cut(s) 36, 390
Bst4CI ACNGT 3 cut(s) 157, 166, 544
Bst6I CTCTTC 1 cut(s) 847
BstAPI GCANNNNNTGC 2 cut(s) 773, 812
BstAUI TGTACA 1 cut(s) 441
BstC8I GCNNGC 1 cut(s) 474
BstDEI CTNAG 2 cut(s) 510, 816
BstF5I GGATG 1 cut(s) 733
BstFNI CGCG 1 cut(s) 9
BstKTI GATC 6 cut(s) 72, 76, 264, 562, 672, 741
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
BstMWI GCNNNNNNNGC 6 cut(s) 116, 234, 773, 776, 812, 818
BstSCI CCNGG 1 cut(s) 675
BstSFI CTRYAG 1 cut(s) 696
BstSLI GKGCMC 1 cut(s) 306
BstUI CGCG 1 cut(s) 9
BstV1I GCAGC 5 cut(s) 19, 493, 682, 779, 799
BstV2I GAAGAC 1 cut(s) 538
BstX2I RGATCY 1 cut(s) 738
BstXI CCANNNNNNTGG 1 cut(s) 218
BstYI RGATCY 1 cut(s) 738
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 2 cut(s) 6, 248
BsuTUI ATCGAT 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 2 cut(s) 282, 705
BtsIMutI CAGTG 7 cut(s) 153, 162, 282, 495, 540, 555, 705
Cac8I GCNNGC 1 cut(s) 474
CaiI CAGNNNCTG 1 cut(s) 812
Cfr10I RCCGGY 1 cut(s) 659
Cfr13I GGNCC 4 cut(s) 4, 96, 246, 752
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 2 cut(s) 442, 484
CspCI CAANNNNNGTGG 2 cut(s) 436, 471
CviAII CATG 4 cut(s) 47, 259, 587, 631
CviQI GTAC 2 cut(s) 442, 484
DdeI CTNAG 2 cut(s) 510, 816
DpnI GATC 6 cut(s) 71, 75, 263, 561, 671, 740
DpnII GATC 6 cut(s) 69, 73, 261, 559, 669, 738
Eam1104I CTCTTC 1 cut(s) 847
EarI CTCTTC 1 cut(s) 847
Ecl136II GAGCTC 1 cut(s) 840
Eco130I CCWWGG 2 cut(s) 36, 390
Eco24I GRGCYC 1 cut(s) 842
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 2 cut(s) 96, 752
Eco53kI GAGCTC 1 cut(s) 840
EcoICRI GAGCTC 1 cut(s) 840
EcoRI GAATTC 1 cut(s) 122
EcoT14I CCWWGG 2 cut(s) 36, 390
EcoT38I GRGCYC 1 cut(s) 842
ErhI CCWWGG 2 cut(s) 36, 390
FaeI CATG 4 cut(s) 50, 262, 590, 634
FaqI GGGAC 1 cut(s) 575
FatI CATG 4 cut(s) 46, 258, 586, 630
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 7 cut(s) 7, 33, 507, 696, 768, 777, 813
FokI GGATG 1 cut(s) 740
FriOI GRGCYC 1 cut(s) 842
Fsp4HI GCNGC 7 cut(s) 7, 33, 507, 696, 768, 777, 813
FspBI CTAG 2 cut(s) 342, 473
GluI GCNGC 7 cut(s) 7, 33, 507, 696, 768, 777, 813
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 2 cut(s) 6, 248
HapII CCGG 3 cut(s) 77, 660, 677
Hin1II CATG 4 cut(s) 50, 262, 590, 634
HinfI GANTC 2 cut(s) 291, 581
HpaII CCGG 3 cut(s) 77, 660, 677
HphI GGTGA 3 cut(s) 629, 649, 779
Hpy166II GTNNAC 4 cut(s) 277, 444, 538, 637
Hpy188I TCNGA 2 cut(s) 89, 692
Hpy188III TCNNGA 3 cut(s) 77, 297, 430
Hpy8I GTNNAC 4 cut(s) 277, 444, 538, 637
HpyAV CCTTC 3 cut(s) 340, 712, 766
HpyCH4III ACNGT 3 cut(s) 157, 166, 544
HpyCH4V TGCA 5 cut(s) 119, 282, 575, 698, 767
HpyF10VI GCNNNNNNNGC 6 cut(s) 116, 234, 773, 776, 812, 818
HpyF3I CTNAG 2 cut(s) 510, 816
Hsp92II CATG 4 cut(s) 50, 262, 590, 634
Kpn2I TCCGGA 1 cut(s) 76
Kzo9I GATC 6 cut(s) 69, 73, 261, 559, 669, 738
LguI GCTCTTC 1 cut(s) 847
LmnI GCTCC 5 cut(s) 395, 461, 692, 809, 837
Lsp1109I GCAGC 5 cut(s) 19, 493, 682, 779, 799
MaeI CTAG 2 cut(s) 342, 473
MaeIII GTNAC 2 cut(s) 188, 319
MalI GATC 6 cut(s) 71, 75, 263, 561, 671, 740
MboI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
MboII GAAGA 3 cut(s) 538, 733, 834
MfeI CAATTG 1 cut(s) 612
MflI RGATCY 1 cut(s) 738
MhlI GDGCHC 3 cut(s) 162, 306, 842
MluCI AATT 2 cut(s) 122, 612
MmeI TCCRAC 1 cut(s) 730
MnlI CCTC 3 cut(s) 17, 73, 293
MroI TCCGGA 1 cut(s) 76
MroXI GAANNNNTTC 1 cut(s) 425
MspI CCGG 3 cut(s) 77, 660, 677
MspR9I CCNGG 1 cut(s) 677
MunI CAATTG 1 cut(s) 612
Mva1269I GAATGC 1 cut(s) 228
MvnI CGCG 1 cut(s) 9
MwoI GCNNNNNNNGC 6 cut(s) 116, 234, 773, 776, 812, 818
NciI CCSGG 1 cut(s) 677
NdeII GATC 6 cut(s) 69, 73, 261, 559, 669, 738
NheI GCTAGC 1 cut(s) 472
NlaIII CATG 4 cut(s) 50, 262, 590, 634
NlaIV GGNNCC 3 cut(s) 523, 603, 754
NmuCI GTSAC 2 cut(s) 188, 319
PciSI GCTCTTC 1 cut(s) 847
PctI GAATGC 1 cut(s) 228
PdmI GAANNNNTTC 1 cut(s) 425
PfeI GAWTC 2 cut(s) 291, 581
PkrI GCNGC 7 cut(s) 8, 34, 508, 697, 769, 778, 814
Psp124BI GAGCTC 1 cut(s) 842
PspN4I GGNNCC 3 cut(s) 523, 603, 754
PspPI GGNCC 4 cut(s) 4, 96, 246, 752
PstI CTGCAG 1 cut(s) 700
PstNI CAGNNNCTG 1 cut(s) 812
PsuI RGATCY 1 cut(s) 738
RsaI GTAC 2 cut(s) 443, 485
RsaNI GTAC 2 cut(s) 442, 484
SacI GAGCTC 1 cut(s) 842
SapI GCTCTTC 1 cut(s) 847
SatI GCNGC 7 cut(s) 7, 33, 507, 696, 768, 777, 813
Sau3AI GATC 6 cut(s) 69, 73, 261, 559, 669, 738
Sau96I GGNCC 4 cut(s) 4, 96, 246, 752
ScrFI CCNGG 1 cut(s) 677
SduI GDGCHC 3 cut(s) 162, 306, 842
SfcI CTRYAG 1 cut(s) 696
SinI GGWCC 2 cut(s) 96, 752
Sse9I AATT 2 cut(s) 122, 612
SsiI CCGC 3 cut(s) 7, 56, 777
SspMI CTAG 2 cut(s) 342, 473
SstI GAGCTC 1 cut(s) 842
StyD4I CCNGG 1 cut(s) 675
StyI CCWWGG 2 cut(s) 36, 390
TaaI ACNGT 3 cut(s) 157, 166, 544
TaqI TCGA 2 cut(s) 72, 682
TasI AATT 2 cut(s) 122, 612
TatI WGTACW 1 cut(s) 441
TauI GCSGC 2 cut(s) 9, 779
TfiI GAWTC 2 cut(s) 291, 581
TscAI CASTG 7 cut(s) 160, 169, 289, 502, 547, 562, 705
TseFI GTSAC 2 cut(s) 188, 319
TseI GCWGC 5 cut(s) 32, 506, 695, 767, 812
Tsp45I GTSAC 2 cut(s) 188, 319
TspDTI ATGAA 3 cut(s) 115, 619, 748
TspRI CASTG 7 cut(s) 160, 169, 289, 502, 547, 562, 705
VpaK11BI GGWCC 2 cut(s) 96, 752
XapI RAATTY 1 cut(s) 122
XmiI GTMKAC 1 cut(s) 537
XmnI GAANNNNTTC 1 cut(s) 425
XspI CTAG 2 cut(s) 342, 473
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.