Rh3DG200200

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr3D
OG0003978
Physical Location & Seq
Forward (+)
18164793 .. 18183818
19026 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh3DG200200.1

Sequence Viewer

Length: 747 bp
ATGGGCAAGGACATTGAAGTTGGAGGGTTTGCTGCCAAGGACTACCATGACCCACCGCCAACACCATTGATCGATCCGGAGGAGTTCGGAAAATGGTCCTTTTACAGAGCCATCATTGCAGAGTTCATCGCTACGCTTTTGTTCTTGTACATCACTGTGCTCACTGTGATTGGATACAAGAGCCAGAGTGACACTCTCAAAGGTGGAGACCAATGTGGTGGTGTTGGGATTCTTGGCATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTAGTTTACTGCACTGCTGGAATCTCTGGAGGGCACATAAACCCAGCTGTGACATTTGGGCTGTTTTTGGCTAGGAAGGTGTCACTGCCCAGAGCTGTATTGTATATTGCGGCTCAGTCCTTGGGAGCAATATGTGGGTGTGGGCTTGTCAAATTGTTCCAGAGTGCTTTATACACAAACTATGGTGGTGGAGCTAATGGGTTAGCTGATGGGTACAGCAAAGGCACCGGTTTGGCTGCTGAGATTATCGGTACCTTTGTTCTTGTCTACACCGTCTTCTCTGCCACTGATCCCAAGAGAAATGCAAGGGATTCCCATGTCCCAGTATTGGCACCACTTCCAATTGGGTTTGCTGTGTTCATGGTTCACCTTGCCACAATCCCAATCACTGGCACAGGGATCAACCCCGCTCGAAGTTTAGGGGCGGCAGTGATTTACAACAATGAGAAGGCTTGGGATGACCATGTATGCTCTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

248

Amino Acids

26.1

Weight (kDa)

6.5

Isoelectric Point (pI)

27.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 29 - 245 8.4e-74 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc65I GGTACC 1 cut(s) 521
AccB1I GGYRCC 3 cut(s) 494, 521, 601
AccB7I CCANNNNNTGG 1 cut(s) 659
AccBSI CCGCTC 1 cut(s) 680
AccI GTMKAC 1 cut(s) 537
AccIII TCCGGA 1 cut(s) 76
AciI CCGC 4 cut(s) 56, 380, 678, 695
AclWI GGATC 3 cut(s) 68, 554, 677
AfaI GTAC 3 cut(s) 149, 485, 523
AfiI CCNNNNNNNGG 2 cut(s) 598, 659
AgeI ACCGGT 1 cut(s) 497
AgsI TTSAA 1 cut(s) 17
AluBI AGCT 4 cut(s) 317, 365, 464, 476
AluI AGCT 4 cut(s) 317, 365, 464, 476
Alw21I GWGCWC 1 cut(s) 162
Alw26I GTCTC 1 cut(s) 201
AlwI GGATC 3 cut(s) 68, 554, 677
Aor13HI TCCGGA 1 cut(s) 76
AoxI GGCC 1 cut(s) 246
ApeKI GCWGC 2 cut(s) 32, 506
AsiGI ACCGGT 1 cut(s) 497
Asp718I GGTACC 1 cut(s) 521
AspS9I GGNCC 2 cut(s) 96, 246
AsuHPI GGTGA 1 cut(s) 629
AvaII GGWCC 1 cut(s) 96
BaeGI GKGCMC 1 cut(s) 306
BaeI ACNNNNGTAYC 2 cut(s) 513, 546
BanI GGYRCC 3 cut(s) 494, 521, 601
BbsI GAAGAC 1 cut(s) 538
Bbv12I GWGCWC 1 cut(s) 162
BbvI GCAGC 2 cut(s) 19, 493
BccI CCATC 2 cut(s) 119, 473
BciVI GTATCC 1 cut(s) 167
BcoDI GTCTC 1 cut(s) 201
BfaI CTAG 2 cut(s) 272, 342
BfuI GTATCC 1 cut(s) 167
BisI GCNGC 4 cut(s) 33, 381, 507, 696
BlsI GCNGC 4 cut(s) 34, 382, 508, 697
Bme18I GGWCC 1 cut(s) 96
BmgT120I GGNCC 2 cut(s) 96, 246
BmiI GGNNCC 3 cut(s) 496, 523, 603
BmrI ACTGGG 1 cut(s) 587
BmuI ACTGGG 1 cut(s) 587
BpiI GAAGAC 1 cut(s) 538
BplI GAGNNNNNCTC 2 cut(s) 178, 210
BpmI CTGGAG 1 cut(s) 318
Bsa29I ATCGAT 1 cut(s) 72
BsaI GGTCTC 1 cut(s) 201
BsaJI CCNNGG 2 cut(s) 36, 390
BsaWI WCCGGW 2 cut(s) 76, 497
Bsc4I CCNNNNNNNGG 2 cut(s) 598, 659
Bse118I RCCGGY 1 cut(s) 497
Bse1I ACTGG 2 cut(s) 593, 664
Bse3DI GCAATG 2 cut(s) 114, 237
BseAI TCCGGA 1 cut(s) 76
BseCI ATCGAT 1 cut(s) 72
BseDI CCNNGG 2 cut(s) 36, 390
BseGI GGATG 1 cut(s) 733
BseLI CCNNNNNNNGG 2 cut(s) 598, 659
BseMI GCAATG 2 cut(s) 114, 237
BseMII CTCAG 2 cut(s) 398, 501
BseNI ACTGG 2 cut(s) 593, 664
BseRI GAGGAG 1 cut(s) 95
BseSI GKGCMC 1 cut(s) 306
BseXI GCAGC 2 cut(s) 19, 493
BseYI CCCAGC 1 cut(s) 313
BsgI GTGCAG 1 cut(s) 265
BshFI GGCC 1 cut(s) 248
BshNI GGYRCC 3 cut(s) 494, 521, 601
BshTI ACCGGT 1 cut(s) 497
BshVI ATCGAT 1 cut(s) 72
BsiHKAI GWGCWC 1 cut(s) 162
BsiSI CCGG 2 cut(s) 77, 498
BslFI GGGAC 1 cut(s) 575
BslI CCNNNNNNNGG 2 cut(s) 598, 659
BsmAI GTCTC 1 cut(s) 201
BsmFI GGGAC 1 cut(s) 575
BsnI GGCC 1 cut(s) 248
Bso31I GGTCTC 1 cut(s) 201
Bsp1286I GDGCHC 2 cut(s) 162, 306
Bsp13I TCCGGA 1 cut(s) 76
Bsp1407I TGTACA 1 cut(s) 147
Bsp143I GATC 5 cut(s) 69, 73, 261, 559, 669
BspACI CCGC 4 cut(s) 56, 380, 678, 695
BspANI GGCC 1 cut(s) 248
BspCNI CTCAG 2 cut(s) 397, 502
BspDI ATCGAT 1 cut(s) 72
BspEI TCCGGA 1 cut(s) 76
BspLI GGNNCC 3 cut(s) 496, 523, 603
BspPI GGATC 3 cut(s) 68, 554, 677
BspT107I GGYRCC 3 cut(s) 494, 521, 601
BspTNI GGTCTC 1 cut(s) 201
BsrBI CCGCTC 1 cut(s) 680
BsrDI GCAATG 2 cut(s) 114, 237
BsrFI RCCGGY 1 cut(s) 497
BsrGI TGTACA 1 cut(s) 147
BsrI ACTGG 2 cut(s) 593, 664
BssAI RCCGGY 1 cut(s) 497
BssECI CCNNGG 2 cut(s) 36, 390
BssMI GATC 5 cut(s) 69, 73, 261, 559, 669
BssT1I CCWWGG 2 cut(s) 36, 390
Bst4CI ACNGT 3 cut(s) 157, 166, 544
BstAUI TGTACA 1 cut(s) 147
BstDEI CTNAG 3 cut(s) 384, 510, 744
BstF5I GGATG 1 cut(s) 733
BstKTI GATC 5 cut(s) 72, 76, 264, 562, 672
BstMAI GTCTC 1 cut(s) 201
BstMBI GATC 5 cut(s) 69, 73, 261, 559, 669
BstMWI GCNNNNNNNGC 1 cut(s) 116
BstSLI GKGCMC 1 cut(s) 306
BstV1I GCAGC 2 cut(s) 19, 493
BstV2I GAAGAC 1 cut(s) 538
BstXI CCANNNNNNTGG 1 cut(s) 218
Bsu15I ATCGAT 1 cut(s) 72
BsuI GTATCC 1 cut(s) 167
BsuRI GGCC 1 cut(s) 248
BsuTUI ATCGAT 1 cut(s) 72
BtgZI GCGATG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 733
BtsI GCAGTG 3 cut(s) 282, 353, 705
BtsIMutI CAGTG 7 cut(s) 153, 162, 282, 353, 555, 657, 705
Cfr10I RCCGGY 1 cut(s) 497
Cfr13I GGNCC 2 cut(s) 96, 246
ClaI ATCGAT 1 cut(s) 72
Csp6I GTAC 3 cut(s) 148, 484, 522
CspAI ACCGGT 1 cut(s) 497
CviAII CATG 5 cut(s) 47, 259, 587, 631, 734
CviQI GTAC 3 cut(s) 148, 484, 522
DdeI CTNAG 3 cut(s) 384, 510, 744
DpnI GATC 5 cut(s) 71, 75, 263, 561, 671
DpnII GATC 5 cut(s) 69, 73, 261, 559, 669
Eco130I CCWWGG 2 cut(s) 36, 390
Eco31I GGTCTC 1 cut(s) 201
Eco47I GGWCC 1 cut(s) 96
EcoT14I CCWWGG 2 cut(s) 36, 390
ErhI CCWWGG 2 cut(s) 36, 390
FaeI CATG 5 cut(s) 50, 262, 590, 634, 737
FaqI GGGAC 1 cut(s) 575
FatI CATG 5 cut(s) 46, 258, 586, 630, 733
FauI CCCGC 1 cut(s) 685
FblI GTMKAC 1 cut(s) 537
Fnu4HI GCNGC 4 cut(s) 33, 381, 507, 696
FokI GGATG 1 cut(s) 740
Fsp4HI GCNGC 4 cut(s) 33, 381, 507, 696
FspBI CTAG 2 cut(s) 272, 342
GluI GCNGC 4 cut(s) 33, 381, 507, 696
GsaI CCCAGC 1 cut(s) 317
GsuI CTGGAG 1 cut(s) 318
HaeIII GGCC 1 cut(s) 248
HapII CCGG 2 cut(s) 77, 498
Hin1II CATG 5 cut(s) 50, 262, 590, 634, 737
HinfI GANTC 3 cut(s) 229, 291, 581
HpaII CCGG 2 cut(s) 77, 498
HphI GGTGA 1 cut(s) 629
Hpy166II GTNNAC 3 cut(s) 277, 538, 637
Hpy188I TCNGA 1 cut(s) 89
Hpy188III TCNNGA 3 cut(s) 77, 297, 430
Hpy8I GTNNAC 3 cut(s) 277, 538, 637
HpyAV CCTTC 2 cut(s) 340, 712
HpyCH4III ACNGT 3 cut(s) 157, 166, 544
HpyCH4V TGCA 3 cut(s) 119, 282, 575
HpyF10VI GCNNNNNNNGC 1 cut(s) 116
HpyF3I CTNAG 3 cut(s) 384, 510, 744
Hsp92II CATG 5 cut(s) 50, 262, 590, 634, 737
Kpn2I TCCGGA 1 cut(s) 76
KpnI GGTACC 1 cut(s) 525
Kzo9I GATC 5 cut(s) 69, 73, 261, 559, 669
LmnI GCTCC 2 cut(s) 395, 461
Lsp1109I GCAGC 2 cut(s) 19, 493
MaeI CTAG 2 cut(s) 272, 342
MaeIII GTNAC 3 cut(s) 188, 319, 351
MalI GATC 5 cut(s) 71, 75, 263, 561, 671
MbiI CCGCTC 1 cut(s) 680
MboI GATC 5 cut(s) 69, 73, 261, 559, 669
MboII GAAGA 1 cut(s) 538
MfeI CAATTG 1 cut(s) 612
MhlI GDGCHC 2 cut(s) 162, 306
MluCI AATT 2 cut(s) 422, 612
MnlI CCTC 3 cut(s) 17, 73, 293
MroI TCCGGA 1 cut(s) 76
MslI CAYNNNNRTG 1 cut(s) 155
MspA1I CMGCKG 1 cut(s) 317
MspI CCGG 2 cut(s) 77, 498
MunI CAATTG 1 cut(s) 612
MwoI GCNNNNNNNGC 1 cut(s) 116
NdeII GATC 5 cut(s) 69, 73, 261, 559, 669
NlaIII CATG 5 cut(s) 50, 262, 590, 634, 737
NlaIV GGNNCC 3 cut(s) 496, 523, 603
NmuCI GTSAC 3 cut(s) 188, 319, 351
PfeI GAWTC 3 cut(s) 229, 291, 581
PflMI CCANNNNNTGG 1 cut(s) 659
PinAI ACCGGT 1 cut(s) 497
PkrI GCNGC 4 cut(s) 34, 382, 508, 697
PspFI CCCAGC 1 cut(s) 313
PspN4I GGNNCC 3 cut(s) 496, 523, 603
PspPI GGNCC 2 cut(s) 96, 246
PvuII CAGCTG 1 cut(s) 317
RsaI GTAC 3 cut(s) 149, 485, 523
RsaNI GTAC 3 cut(s) 148, 484, 522
RseI CAYNNNNRTG 1 cut(s) 155
SatI GCNGC 4 cut(s) 33, 381, 507, 696
Sau3AI GATC 5 cut(s) 69, 73, 261, 559, 669
Sau96I GGNCC 2 cut(s) 96, 246
SduI GDGCHC 2 cut(s) 162, 306
SetI ASST 8 cut(s) 205, 319, 351, 367, 466, 478, 527, 642
SinI GGWCC 1 cut(s) 96
SmiMI CAYNNNNRTG 1 cut(s) 155
Sse9I AATT 2 cut(s) 422, 612
SsiI CCGC 4 cut(s) 56, 380, 678, 695
SspMI CTAG 2 cut(s) 272, 342
StyI CCWWGG 2 cut(s) 36, 390
TaaI ACNGT 3 cut(s) 157, 166, 544
TaqI TCGA 2 cut(s) 72, 682
TasI AATT 2 cut(s) 422, 612
TatI WGTACW 1 cut(s) 147
TauI GCSGC 2 cut(s) 383, 698
TfiI GAWTC 3 cut(s) 229, 291, 581
TscAI CASTG 7 cut(s) 160, 169, 289, 360, 562, 664, 705
TseFI GTSAC 3 cut(s) 188, 319, 351
TseI GCWGC 2 cut(s) 32, 506
Tsp45I GTSAC 3 cut(s) 188, 319, 351
TspDTI ATGAA 2 cut(s) 115, 619
TspRI CASTG 7 cut(s) 160, 169, 289, 360, 562, 664, 705
Van91I CCANNNNNTGG 1 cut(s) 659
VpaK11BI GGWCC 1 cut(s) 96
XmiI GTMKAC 1 cut(s) 537
XspI CTAG 2 cut(s) 272, 342
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.