MD12G1119200.v1.1

Belongs to the MIP aquaporin (TC 1.A.8) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr12
OG0003978
Physical Location & Seq
Forward (+)
19099490 .. 19101465
1976 bp
Loading structure...
UTR
Exon/CDS
Intron
MD12G1119200.v1.1.491

Sequence Viewer

Length: 858 bp
ATGGGCAAAGACATTGAAGTTGGAGGTGGGTACTCAGCCAAGGACTACCAGGACCCTCCGCCAGAGCCGCTAATCGATGCAGAGGAGCTAGGGAAGTGGTCGTTCTACAGAGCCATCATAGCTGAGTTCATTGCCACCCTTTTGTTCTTGTACATCACTGTGCTCACTGTGATTGGATACAAGAGCCAAACTGACACTGCTCTAAACGGAGACCAATGTGGTGGTGTTGGCATTCTTGGTATTGCTTGGGCCTTTGGTGGCATGATCTTTGTCCTTGTTTACTGCACAGCTGGCATCTCTGGGGGGCACATAAACCCTGCAGTCACATTCGGGCTGCTTTTGGCAAGGAAGGTGTCACTGGTGAGAGCCATATTGTACATGACGGCTCAGTCGTTGGGAGCCATATGTGGTGTTGGGCTAGTGAAAGCTTTCCAAAGTTCTTACTATACTAAGTATGGCGGTGGAGCCAATGAGCTTGCCGATGGCTATAGCACAGGCACTGGATTGGGTGCTGAGATTGTTGGCACATTTGTTCTTGTCTACACTGTCTTCTCTGCCACTGATCCCAAGAGAAATGCAAGAGACTCCCATGTCCCAGTATTGGCACCACTGCCAATTGGGTTTGCTGTGTTTATTGTTCACCTAGCCACAATCCCAATCACTGGTACTGGTATCAACCCAGCTAGAAGTCTTGGAGCTGCAGTGATCTACAACAATGACAAGGCTTGGAATGACCAATGGATCTTCTGGGTTGGACCATTCATTGGAGCTGCCATTGCTGCCCTGTATCACCAATACATCTTGAGAGCGGGAGCTGTTAAAGCTCTGGGTTCTTTCAGGAGCTCCCCCAACATATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
MIP

Protein Analysis

286

Amino Acids

30.16

Weight (kDa)

6.89

Isoelectric Point (pI)

33.31

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
MIP PF00230 30 - 264 1.6e-83 Major intrinsic protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 388, 590
AccB1I GGYRCC 1 cut(s) 604
AccB7I CCANNNNNTGG 2 cut(s) 662, 764
AccBSI CCGCTC 1 cut(s) 809
AccI GTMKAC 1 cut(s) 540
AciI CCGC 4 cut(s) 59, 68, 459, 809
AclWI GGATC 2 cut(s) 557, 749
AfaI GTAC 4 cut(s) 32, 152, 377, 667
AfiI CCNNNNNNNGG 3 cut(s) 601, 662, 764
AgsI TTSAA 1 cut(s) 17
AjnI CCWGG 1 cut(s) 48
Alw21I GWGCWC 2 cut(s) 165, 845
Alw26I GTCTC 2 cut(s) 204, 576
AlwI GGATC 2 cut(s) 557, 749
AlwNI CAGNNNCTG 1 cut(s) 500
AoxI GGCC 1 cut(s) 249
ApeKI GCWGC 4 cut(s) 334, 698, 770, 779
Asp700I GAANNNNTTC 1 cut(s) 428
AspS9I GGNCC 3 cut(s) 52, 249, 755
AsuHPI GGTGA 3 cut(s) 373, 632, 782
AvaII GGWCC 2 cut(s) 52, 755
BaeGI GKGCMC 1 cut(s) 309
BanI GGYRCC 1 cut(s) 604
BanII GRGCYC 1 cut(s) 845
BbsI GAAGAC 1 cut(s) 541
Bbv12I GWGCWC 2 cut(s) 165, 845
BbvI GCAGC 4 cut(s) 321, 685, 757, 766
BccI CCATC 2 cut(s) 122, 476
BceAI ACGGC 1 cut(s) 399
BciT130I CCWGG 1 cut(s) 50
BciVI GTATCC 1 cut(s) 170
BcoDI GTCTC 2 cut(s) 204, 576
BfaI CTAG 4 cut(s) 89, 419, 644, 684
BfmI CTRYAG 4 cut(s) 106, 318, 487, 699
BfuI GTATCC 1 cut(s) 170
BisI GCNGC 5 cut(s) 68, 335, 699, 771, 780
BlsI GCNGC 5 cut(s) 69, 336, 700, 772, 781
Bme1390I CCNGG 1 cut(s) 50
Bme18I GGWCC 2 cut(s) 52, 755
BmgT120I GGNCC 3 cut(s) 52, 249, 755
BmiI GGNNCC 4 cut(s) 54, 400, 466, 606
BmrFI CCNGG 1 cut(s) 50
BmrI ACTGGG 1 cut(s) 590
BmsI GCATC 2 cut(s) 67, 303
BmuI ACTGGG 1 cut(s) 590
BpiI GAAGAC 1 cut(s) 541
BpuEI CTTGAG 1 cut(s) 823
Bsa29I ATCGAT 1 cut(s) 75
BsaI GGTCTC 1 cut(s) 204
BsaJI CCNNGG 1 cut(s) 39
BsaXI ACNNNNNCTCC 2 cut(s) 687, 717
Bsc4I CCNNNNNNNGG 3 cut(s) 601, 662, 764
Bse1I ACTGG 5 cut(s) 363, 505, 596, 667, 673
Bse3DI GCAATG 2 cut(s) 129, 774
BseBI CCWGG 1 cut(s) 50
BseCI ATCGAT 1 cut(s) 75
BseDI CCNNGG 1 cut(s) 39
BseLI CCNNNNNNNGG 3 cut(s) 601, 662, 764
BseMI GCAATG 2 cut(s) 129, 774
BseMII CTCAG 4 cut(s) 48, 114, 401, 504
BseNI ACTGG 5 cut(s) 363, 505, 596, 667, 673
BseRI GAGGAG 1 cut(s) 98
BseSI GKGCMC 1 cut(s) 309
BseXI GCAGC 4 cut(s) 321, 685, 757, 766
BseYI CCCAGC 1 cut(s) 679
BsgI GTGCAG 1 cut(s) 268
BshFI GGCC 1 cut(s) 251
BshNI GGYRCC 1 cut(s) 604
BshVI ATCGAT 1 cut(s) 75
BsiHKAI GWGCWC 2 cut(s) 165, 845
BslFI GGGAC 1 cut(s) 578
BslI CCNNNNNNNGG 3 cut(s) 601, 662, 764
BsmAI GTCTC 2 cut(s) 204, 576
BsmFI GGGAC 1 cut(s) 578
BsmI GAATGC 1 cut(s) 231
BsnI GGCC 1 cut(s) 251
Bso31I GGTCTC 1 cut(s) 204
Bsp1286I GDGCHC 3 cut(s) 165, 309, 845
Bsp1407I TGTACA 2 cut(s) 150, 375
Bsp143I GATC 4 cut(s) 264, 562, 705, 741
BspACI CCGC 4 cut(s) 59, 68, 459, 809
BspANI GGCC 1 cut(s) 251
BspCNI CTCAG 4 cut(s) 47, 115, 400, 505
BspDI ATCGAT 1 cut(s) 75
BspLI GGNNCC 4 cut(s) 54, 400, 466, 606
BspMAI CTGCAG 2 cut(s) 322, 703
BspPI GGATC 2 cut(s) 557, 749
BspT107I GGYRCC 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 204
BsrBI CCGCTC 1 cut(s) 809
BsrDI GCAATG 2 cut(s) 129, 774
BsrGI TGTACA 2 cut(s) 150, 375
BsrI ACTGG 5 cut(s) 363, 505, 596, 667, 673
BssECI CCNNGG 1 cut(s) 39
BssMI GATC 4 cut(s) 264, 562, 705, 741
BssT1I CCWWGG 1 cut(s) 39
Bst2UI CCWGG 1 cut(s) 50
Bst4CI ACNGT 3 cut(s) 160, 169, 547
BstAUI TGTACA 2 cut(s) 150, 375
BstC8I GCNNGC 2 cut(s) 292, 477
BstDEI CTNAG 5 cut(s) 34, 123, 387, 450, 513
BstKTI GATC 4 cut(s) 267, 565, 708, 744
BstMAI GTCTC 2 cut(s) 204, 576
BstMBI GATC 4 cut(s) 264, 562, 705, 741
BstMWI GCNNNNNNNGC 6 cut(s) 67, 119, 291, 776, 779, 821
BstNI CCWGG 1 cut(s) 50
BstSCI CCNGG 1 cut(s) 48
BstSFI CTRYAG 4 cut(s) 106, 318, 487, 699
BstSLI GKGCMC 1 cut(s) 309
BstV1I GCAGC 4 cut(s) 321, 685, 757, 766
BstV2I GAAGAC 1 cut(s) 541
BstX2I RGATCY 1 cut(s) 741
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 1 cut(s) 741
Bsu15I ATCGAT 1 cut(s) 75
BsuI GTATCC 1 cut(s) 170
BsuRI GGCC 1 cut(s) 251
BsuTUI ATCGAT 1 cut(s) 75
BtsI GCAGTG 3 cut(s) 195, 608, 708
Cac8I GCNNGC 2 cut(s) 292, 477
CaiI CAGNNNCTG 1 cut(s) 500
Cfr13I GGNCC 3 cut(s) 52, 249, 755
ClaI ATCGAT 1 cut(s) 75
Csp6I GTAC 4 cut(s) 31, 151, 376, 666
CviAII CATG 3 cut(s) 262, 379, 590
CviQI GTAC 4 cut(s) 31, 151, 376, 666
DdeI CTNAG 5 cut(s) 34, 123, 387, 450, 513
DpnI GATC 4 cut(s) 266, 564, 707, 743
DpnII GATC 4 cut(s) 264, 562, 705, 741
DrdI GACNNNNNNGTC 2 cut(s) 388, 590
DseDI GACNNNNNNGTC 2 cut(s) 388, 590
EciI GGCGGA 1 cut(s) 48
Ecl136II GAGCTC 1 cut(s) 843
Eco130I CCWWGG 1 cut(s) 39
Eco24I GRGCYC 1 cut(s) 845
Eco31I GGTCTC 1 cut(s) 204
Eco47I GGWCC 2 cut(s) 52, 755
Eco53kI GAGCTC 1 cut(s) 843
EcoICRI GAGCTC 1 cut(s) 843
EcoO109I RGGNCCY 1 cut(s) 52
EcoRII CCWGG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 39
EcoT38I GRGCYC 1 cut(s) 845
ErhI CCWWGG 1 cut(s) 39
FaeI CATG 3 cut(s) 265, 382, 593
FaqI GGGAC 1 cut(s) 578
FatI CATG 3 cut(s) 261, 378, 589
FauI CCCGC 1 cut(s) 802
FauNDI CATATG 2 cut(s) 404, 854
FblI GTMKAC 1 cut(s) 540
Fnu4HI GCNGC 5 cut(s) 68, 335, 699, 771, 780
FriOI GRGCYC 1 cut(s) 845
Fsp4HI GCNGC 5 cut(s) 68, 335, 699, 771, 780
FspBI CTAG 4 cut(s) 89, 419, 644, 684
GluI GCNGC 5 cut(s) 68, 335, 699, 771, 780
GsaI CCCAGC 1 cut(s) 683
HaeIII GGCC 1 cut(s) 251
Hin1II CATG 3 cut(s) 265, 382, 593
HindIII AAGCTT 1 cut(s) 426
HinfI GANTC 1 cut(s) 584
HphI GGTGA 3 cut(s) 373, 632, 782
Hpy166II GTNNAC 3 cut(s) 280, 541, 640
Hpy188III TCNNGA 2 cut(s) 802, 838
Hpy8I GTNNAC 3 cut(s) 280, 541, 640
HpyAV CCTTC 1 cut(s) 343
HpyCH4III ACNGT 3 cut(s) 160, 169, 547
HpyCH4V TGCA 5 cut(s) 80, 285, 320, 578, 701
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 119, 291, 776, 779, 821
HpyF3I CTNAG 5 cut(s) 34, 123, 387, 450, 513
Hsp92II CATG 3 cut(s) 265, 382, 593
Kzo9I GATC 4 cut(s) 264, 562, 705, 741
LmnI GCTCC 8 cut(s) 85, 398, 464, 695, 767, 812, 840, 848
Lsp1109I GCAGC 4 cut(s) 321, 685, 757, 766
LweI GCATC 2 cut(s) 67, 303
MaeI CTAG 4 cut(s) 89, 419, 644, 684
MaeIII GTNAC 2 cut(s) 322, 354
MalI GATC 4 cut(s) 266, 564, 707, 743
MbiI CCGCTC 1 cut(s) 809
MboI GATC 4 cut(s) 264, 562, 705, 741
MboII GAAGA 2 cut(s) 541, 736
MfeI CAATTG 1 cut(s) 615
MflI RGATCY 1 cut(s) 741
MhlI GDGCHC 3 cut(s) 165, 309, 845
MluCI AATT 1 cut(s) 615
MlyI GAGTC 1 cut(s) 578
MmeI TCCRAC 1 cut(s) 733
MnlI CCTC 3 cut(s) 17, 66, 76
MroXI GAANNNNTTC 1 cut(s) 428
MseI TTAA 1 cut(s) 819
MslI CAYNNNNRTG 1 cut(s) 158
MspA1I CMGCKG 1 cut(s) 290
MspR9I CCNGG 1 cut(s) 50
MunI CAATTG 1 cut(s) 615
Mva1269I GAATGC 1 cut(s) 231
MvaI CCWGG 1 cut(s) 50
MwoI GCNNNNNNNGC 6 cut(s) 67, 119, 291, 776, 779, 821
NdeI CATATG 2 cut(s) 404, 854
NdeII GATC 4 cut(s) 264, 562, 705, 741
NlaIII CATG 3 cut(s) 265, 382, 593
NlaIV GGNNCC 4 cut(s) 54, 400, 466, 606
NmuCI GTSAC 2 cut(s) 322, 354
PcsI WCGNNNNNNNCGW 1 cut(s) 389
PctI GAATGC 1 cut(s) 231
PdmI GAANNNNTTC 1 cut(s) 428
PflMI CCANNNNNTGG 2 cut(s) 662, 764
PkrI GCNGC 5 cut(s) 69, 336, 700, 772, 781
PleI GAGTC 1 cut(s) 578
PpsI GAGTC 1 cut(s) 578
PpuMI RGGWCCY 1 cut(s) 52
Psp124BI GAGCTC 1 cut(s) 845
Psp5II RGGWCCY 1 cut(s) 52
Psp6I CCWGG 1 cut(s) 48
PspFI CCCAGC 1 cut(s) 679
PspGI CCWGG 1 cut(s) 48
PspN4I GGNNCC 4 cut(s) 54, 400, 466, 606
PspPI GGNCC 3 cut(s) 52, 249, 755
PspPPI RGGWCCY 1 cut(s) 52
PstI CTGCAG 2 cut(s) 322, 703
PstNI CAGNNNCTG 1 cut(s) 500
PsuI RGATCY 1 cut(s) 741
PvuII CAGCTG 1 cut(s) 290
RsaI GTAC 4 cut(s) 32, 152, 377, 667
RsaNI GTAC 4 cut(s) 31, 151, 376, 666
RseI CAYNNNNRTG 1 cut(s) 158
SacI GAGCTC 1 cut(s) 845
SaqAI TTAA 1 cut(s) 819
SatI GCNGC 5 cut(s) 68, 335, 699, 771, 780
Sau3AI GATC 4 cut(s) 264, 562, 705, 741
Sau96I GGNCC 3 cut(s) 52, 249, 755
SchI GAGTC 1 cut(s) 578
ScrFI CCNGG 1 cut(s) 50
SduI GDGCHC 3 cut(s) 165, 309, 845
SfaNI GCATC 2 cut(s) 67, 303
SfcI CTRYAG 4 cut(s) 106, 318, 487, 699
SinI GGWCC 2 cut(s) 52, 755
SmiMI CAYNNNNRTG 1 cut(s) 158
SmlI CTYRAG 1 cut(s) 802
SmoI CTYRAG 1 cut(s) 802
Sse9I AATT 1 cut(s) 615
SsiI CCGC 4 cut(s) 59, 68, 459, 809
SspMI CTAG 4 cut(s) 89, 419, 644, 684
SstI GAGCTC 1 cut(s) 845
StyD4I CCNGG 1 cut(s) 48
StyI CCWWGG 1 cut(s) 39
TaaI ACNGT 3 cut(s) 160, 169, 547
TaqI TCGA 1 cut(s) 75
TasI AATT 1 cut(s) 615
TatI WGTACW 2 cut(s) 150, 375
TauI GCSGC 1 cut(s) 70
Tru1I TTAA 1 cut(s) 819
Tru9I TTAA 1 cut(s) 819
TseFI GTSAC 2 cut(s) 322, 354
TseI GCWGC 4 cut(s) 334, 698, 770, 779
Tsp45I GTSAC 2 cut(s) 322, 354
TspDTI ATGAA 2 cut(s) 118, 751
TspGWI ACGGA 1 cut(s) 222
Van91I CCANNNNNTGG 2 cut(s) 662, 764
VpaK11BI GGWCC 2 cut(s) 52, 755
XmiI GTMKAC 1 cut(s) 540
XmnI GAANNNNTTC 1 cut(s) 428
XspI CTAG 4 cut(s) 89, 419, 644, 684
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.